FastQCFastQC Report
Thu 26 May 2016
SRR1364365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637947
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.13919864317954123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.13498605266226563No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.062.940451
GTATCAA40400.051.5992771
ATCAACG50750.040.363283
CAACGCA51350.040.0076185
TCAACGC51600.039.813784
TATCAAC53500.038.733752
AACGCAG53450.038.518926
GTACATG68000.036.787251
ACATGGG70550.034.099533
TACATGG72850.033.758612
GTGGTAT13150.033.0638961
ACGCAGA63500.032.5123567
CGCAGAG64450.031.848498
CATGGGG37800.029.6162514
TGGTATC13900.029.5595872
GCAGAGT74300.027.3860869
CATGGGA37850.027.0599274
GAGTACT38350.026.52915612-13
CAGAGTA74300.023.98284510-11
GTCTATG10750.023.8241231
Produced by FastQC (version 0.11.5)