FastQCFastQC Report
Thu 26 May 2016
SRR1364364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922736
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24900.047.1098481
ATCAACG24750.044.9778023
GGTATCA16300.043.836761
CAACGCA25600.043.251865
TCAACGC26250.041.9540824
AACGCAG27050.040.7069446
TATCAAC28650.039.0629542
GTACATG65600.038.0325131
ACATGGG68950.034.4485133
TACATGG70000.034.356992
ACGCAGA36650.029.8709957
CATGGGG43700.029.5603874
CGCAGAG36800.029.1010048
GTAAGGT10500.024.9456714
TAAGGTG11750.023.8117775
TATTCCG2552.467641E-823.3448815
CATGGGA34000.023.2865184
GGTAAGG11950.022.4169453
ATGGGGA24900.021.5166665
GCAGAGT49250.021.3820979
Produced by FastQC (version 0.11.5)