FastQCFastQC Report
Thu 26 May 2016
SRR1364364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922736
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.10849123332584401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.058.148941
GTATCAA38600.047.97951
ATCAACG46400.038.7508473
TCAACGC47200.038.094054
CAACGCA47700.037.6947445
AACGCAG49450.036.3418356
TATCAAC50550.035.805082
GTACATG63400.034.095731
TACATGG65750.032.4174962
ACATGGG67500.030.7832053
ACGCAGA60500.029.6995627
CGCAGAG61100.029.4079118
TAAGGTG12400.028.8085775
TATTCCG2603.6379788E-1127.4789475
GTGGTAT11600.026.6948811
CATGGGG41450.026.5729264
GTAAGGT11350.026.228074
AGGTAAG11600.023.6097872
CATGGGA34500.023.2973694
GCAGAGT76000.023.2509849
Produced by FastQC (version 0.11.5)