FastQCFastQC Report
Thu 26 May 2016
SRR1364363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718294
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15800.043.714231
GTATCAA23400.042.49351
ATCAACG23750.041.109193
TCAACGC24650.039.849764
CAACGCA24950.039.130855
AACGCAG25750.038.1429946
GTACATG56750.034.833261
TATCAAC28600.034.137882
TACATGG60150.031.7707962
ACATGGG61150.031.2512423
ACGCAGA32400.030.1138827
CGCAGAG33550.028.7261738
CATGGGG36150.026.0200124
GTATAGG5500.025.9818731
CATGGGA30300.024.7563484
TAGCCCT11100.023.0623824
GTGTAAG11650.022.9989761
TAAGGTA6750.022.0493054
GTATAGA6550.021.8168411
TAAGGTG9300.021.7641625
Produced by FastQC (version 0.11.5)