##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364363_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1718294 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.39299735668052 31.0 31.0 34.0 26.0 34.0 2 30.397714826449956 31.0 31.0 34.0 26.0 34.0 3 30.512472836429623 31.0 31.0 34.0 26.0 34.0 4 33.95724131027635 37.0 35.0 37.0 30.0 37.0 5 34.009536784741144 37.0 35.0 37.0 30.0 37.0 6 34.1537105990011 37.0 35.0 37.0 30.0 37.0 7 34.012982062441004 37.0 35.0 37.0 30.0 37.0 8 33.95031350863123 37.0 35.0 37.0 30.0 37.0 9 35.47614494376399 39.0 35.0 39.0 30.0 39.0 10-11 35.3563511250112 39.0 35.0 39.0 29.0 39.0 12-13 35.36170090799362 39.0 35.0 39.0 29.0 39.0 14-15 36.57260078892203 40.0 37.0 41.0 29.0 41.0 16-17 36.56052805864421 40.0 37.0 41.0 29.0 41.0 18-19 36.538904867269515 40.0 37.0 41.0 29.0 41.0 20-21 36.56366285397027 40.0 37.0 41.0 29.0 41.0 22-23 36.50328698115689 40.0 37.0 41.0 28.5 41.0 24-25 36.48760543888299 40.0 37.0 41.0 28.5 41.0 26-27 36.42715827442801 40.0 36.0 41.0 28.5 41.0 28-29 36.42802133977072 40.0 36.0 41.0 29.0 41.0 30-31 36.317066229644055 40.0 36.0 41.0 28.5 41.0 32-33 36.276856579840235 40.0 36.0 41.0 29.0 41.0 34-35 36.259879566593376 40.0 36.0 41.0 29.0 41.0 36-37 36.18543421556497 40.0 36.0 41.0 28.0 41.0 38-39 36.07718382302447 40.0 36.0 41.0 28.0 41.0 40-41 36.0131057316152 40.0 36.0 41.0 28.0 41.0 42-43 35.85658420503127 40.0 35.0 41.0 27.0 41.0 44-45 35.703012697477845 40.0 35.0 41.0 27.0 41.0 46-47 35.57529328508393 39.5 35.0 41.0 27.0 41.0 48-49 35.41186083405983 39.0 35.0 41.0 26.0 41.0 50-51 35.27606393318024 39.0 35.0 41.0 26.0 41.0 52-53 35.106760251738066 39.0 34.5 41.0 26.0 41.0 54-55 34.91895595282297 39.0 34.0 41.0 26.0 41.0 56-57 34.71548378798972 38.5 34.0 40.0 25.0 41.0 58-59 34.4883003141488 38.0 34.0 40.0 25.0 41.0 60-61 34.236594261517524 38.0 34.0 40.0 24.0 41.0 62-63 33.9617341386282 37.0 33.0 40.0 23.0 41.0 64-65 33.63053615970259 37.0 33.0 40.0 23.0 41.0 66-67 33.316937031730305 36.0 33.0 39.5 22.0 41.0 68-69 32.999700284119015 36.0 32.5 39.0 22.0 41.0 70-71 32.644793906048676 35.0 32.0 39.0 21.5 40.5 72-73 32.29797578295682 35.0 32.0 38.0 20.0 40.0 74-75 31.95235070366305 35.0 32.0 37.0 20.0 39.5 76-77 31.73750126579037 35.0 32.0 37.0 20.0 39.0 78-79 31.593957727839356 35.0 32.0 36.5 20.0 39.0 80-81 31.34236545084834 35.0 32.0 36.0 19.5 38.0 82-83 31.05954714385315 35.0 32.0 36.0 18.0 37.0 84-85 30.822047914966824 35.0 31.0 35.0 18.0 37.0 86-87 30.631450438632736 35.0 31.0 35.0 17.5 36.0 88-89 30.382133965433155 35.0 31.0 35.0 12.5 36.0 90-91 30.262772552310608 35.0 31.0 35.0 11.5 36.0 92-93 30.12989482591454 35.0 31.0 35.0 10.0 35.5 94-95 30.031080827844363 34.0 31.0 35.0 7.0 35.0 96-97 29.941890037444118 34.0 31.0 35.0 7.0 35.0 98-99 29.81570237689243 34.0 31.0 35.0 6.5 35.0 100-101 29.724520949267124 34.0 31.0 35.0 2.0 35.0 102-103 28.690830847340443 33.5 29.0 34.5 2.0 35.0 104-105 29.42538063916885 34.0 30.0 35.0 2.0 35.0 106-107 29.798665420469373 34.5 31.0 35.0 2.0 35.0 108-109 29.861188772119323 35.0 31.0 35.0 2.0 35.0 110-111 29.80441909242539 35.0 31.0 35.0 2.0 35.0 112-113 29.716612232830936 35.0 31.0 35.0 2.0 35.0 114-115 29.62553061350386 35.0 31.0 35.0 2.0 35.0 116-117 29.502070076482838 35.0 31.0 35.0 2.0 35.0 118-119 29.342022378009815 35.0 31.0 35.0 2.0 35.0 120-121 29.173581179937777 35.0 31.0 35.0 2.0 35.0 122-123 28.997131457131317 35.0 31.0 35.0 2.0 35.0 124-125 28.16543996545411 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0732669631472298 1101 2 0.02882545702325956 1101 3 -0.03735652278407642 1101 4 0.17175642754247633 1101 5 -0.013647475115725172 1101 6 0.038733065422185575 1101 7 0.13579269609828515 1101 8 -0.056053669568221665 1101 9 0.12970850510656362 1101 10-11 0.2734149095297269 1101 12-13 0.3585352961788786 1101 14-15 0.42932362136748736 1101 16-17 0.3493451682722437 1101 18-19 0.0883240698915273 1101 20-21 0.30299845988060525 1101 22-23 0.33081276020612194 1101 24-25 0.4014720826868725 1101 26-27 0.418864177413127 1101 28-29 0.5112544032335009 1101 30-31 0.5378593709129262 1101 32-33 0.37617908767635555 1101 34-35 0.4703260063258554 1101 36-37 0.2805314996162025 1101 38-39 0.48600955385956723 1101 40-41 0.4368770856733093 1101 42-43 0.5820111089593283 1101 44-45 0.46914137853747206 1101 46-47 0.5171006807641234 1101 48-49 0.3994308494873948 1101 50-51 0.33938322964380774 1101 52-53 0.27469260705458254 1101 54-55 0.4493156477367748 1101 56-57 0.3500881205373929 1101 58-59 0.35589069599743794 1101 60-61 0.3755656679197017 1101 62-63 0.37187086859363916 1101 64-65 0.45741886072628546 1101 66-67 0.3383061876247737 1101 68-69 0.32342146174332953 1101 70-71 0.3775148269310833 1101 72-73 0.3737126016079415 1101 74-75 0.29892192030159137 1101 76-77 0.28808094582874944 1101 78-79 0.37343650020969577 1101 80-81 0.39209232895952795 1101 82-83 0.30432843889905214 1101 84-85 0.3170173285961191 1101 86-87 0.3400811917162301 1101 88-89 0.45219258078273583 1101 90-91 0.4355886545298482 1101 92-93 0.4722919847038334 1101 94-95 0.4845707299513258 1101 96-97 0.40472211474034836 1101 98-99 0.34528763239033466 1101 100-101 0.3464858078072801 1101 102-103 0.27601407011339063 1101 104-105 0.34053059155192145 1101 106-107 0.21885291105531834 1101 108-109 0.36582722939635204 1101 110-111 0.4200943318749282 1101 112-113 0.39452997833393155 1101 114-115 0.3104366505662952 1101 116-117 0.3646456892924945 1101 118-119 0.4957473785770894 1101 120-121 0.4000861863778411 1101 122-123 0.3018980746014819 1101 124-125 0.13181315901356783 1102 1 0.1203606319845072 1102 2 0.08123587219998285 1102 3 0.11479746524618761 1102 4 0.2162011333805438 1102 5 0.28197522723431234 1102 6 0.17735475774725273 1102 7 0.15902342720580265 1102 8 0.15908023715816455 1102 9 0.28174878690980165 1102 10-11 0.293551811827065 1102 12-13 0.26980828836216375 1102 14-15 0.25852659560871416 1102 16-17 0.3948328346754195 1102 18-19 0.270071240566935 1102 20-21 0.40495561851098216 1102 22-23 0.33074445805181796 1102 24-25 0.33447362909155487 1102 26-27 0.39286630724839 1102 28-29 0.3455653748649823 1102 30-31 0.2665191430613305 1102 32-33 0.40156161110768807 1102 34-35 0.33553660594191825 1102 36-37 0.20790419569279095 1102 38-39 0.25455927406382983 1102 40-41 0.16853462447458156 1102 42-43 0.10840099114400203 1102 44-45 0.1704595081719873 1102 46-47 0.16355288964481218 1102 48-49 0.22879379662732902 1102 50-51 0.2656502306852033 1102 52-53 0.23350954473416152 1102 54-55 0.23201677444785673 1102 56-57 0.12291272436921474 1102 58-59 0.17740509042159402 1102 60-61 0.1854588154335346 1102 62-63 0.2729726427244472 1102 64-65 0.1312548226339203 1102 66-67 0.10168203924520469 1102 68-69 0.06887572204119152 1102 70-71 -0.024836147335228986 1102 72-73 0.05610052647714525 1102 74-75 -0.03305258440976644 1102 76-77 0.061535246904014684 1102 78-79 0.17812111834497202 1102 80-81 0.1420228505339125 1102 82-83 0.11330723151144184 1102 84-85 0.08394191235290904 1102 86-87 0.19701052760912674 1102 88-89 0.23368222645844838 1102 90-91 0.03084749695340605 1102 92-93 -0.035160086161024395 1102 94-95 -0.030327059913293652 1102 96-97 -0.07739173891268436 1102 98-99 0.025520236121781892 1102 100-101 -0.06013636443648096 1102 102-103 -0.2637537809800712 1102 104-105 -0.0690740558176266 1102 106-107 0.014413349443795909 1102 108-109 -0.02791783026412986 1102 110-111 0.04449270830684071 1102 112-113 0.02443934270828052 1102 114-115 -0.02121061546549896 1102 116-117 -0.02917912156307878 1102 118-119 -0.14346989912331765 1102 120-121 -0.1290010427660846 1102 122-123 -0.01560288209918781 1102 124-125 0.0591603692116891 1103 1 0.45063739368110944 1103 2 0.4746707283133276 1103 3 0.3210015391739205 1103 4 0.531837072703766 1103 5 0.476661675328117 1103 6 0.4052729978874581 1103 7 0.3064557003703712 1103 8 0.4811163311992175 1103 9 0.5155553112767848 1103 10-11 0.6435159238297032 1103 12-13 0.6149800340980889 1103 14-15 0.7033059638504113 1103 16-17 0.5220009586076131 1103 18-19 0.5690515711066411 1103 20-21 0.6120264330713638 1103 22-23 0.6051660320589818 1103 24-25 0.6884842785887386 1103 26-27 0.7207752630850237 1103 28-29 0.6976779086439606 1103 30-31 0.8415043915058931 1103 32-33 0.6788098083025886 1103 34-35 0.8073063854412581 1103 36-37 0.6079014505000373 1103 38-39 0.5673292629997704 1103 40-41 0.5954676180209049 1103 42-43 0.6783964662026918 1103 44-45 0.6451779022897455 1103 46-47 0.6503030163400823 1103 48-49 0.6498655723136224 1103 50-51 0.6318890174210452 1103 52-53 0.7353224515200623 1103 54-55 0.6604559997359587 1103 56-57 0.625284617173449 1103 58-59 0.6351770087036854 1103 60-61 0.549585423106322 1103 62-63 0.5113937127265658 1103 64-65 0.5653183772390449 1103 66-67 0.5693601706387668 1103 68-69 0.47093495589799517 1103 70-71 0.5298766237641175 1103 72-73 0.5250581798740441 1103 74-75 0.5322825403931724 1103 76-77 0.5672185670203298 1103 78-79 0.7011393282263718 1103 80-81 0.5504046642796752 1103 82-83 0.547139603873088 1103 84-85 0.5438238505576933 1103 86-87 0.615716887912285 1103 88-89 0.695719063860686 1103 90-91 0.6371369648826146 1103 92-93 0.6018150846501094 1103 94-95 0.596804204408361 1103 96-97 0.5632779524011902 1103 98-99 0.5270502184075774 1103 100-101 0.558586898851253 1103 102-103 0.3347446602748114 1103 104-105 0.5325466566308386 1103 106-107 0.616120504619051 1103 108-109 0.62804551493522 1103 110-111 0.6730214762304065 1103 112-113 0.620876036250074 1103 114-115 0.5554500487909522 1103 116-117 0.5650775342184389 1103 118-119 0.517019698292625 1103 120-121 0.2840244650097503 1103 122-123 0.2304569180692866 1103 124-125 0.22661758970094326 1104 1 0.3124520715512702 1104 2 0.42373306648631015 1104 3 0.4612953263103101 1104 4 0.4687650472361966 1104 5 0.4434372969726468 1104 6 0.424030967941988 1104 7 0.4819223380889639 1104 8 0.40233897898189497 1104 9 0.5485920846862982 1104 10-11 0.5584141232955702 1104 12-13 0.48322377094878277 1104 14-15 0.6010682107927963 1104 16-17 0.5376464852946938 1104 18-19 0.539658341357331 1104 20-21 0.6226236668687832 1104 22-23 0.5962925074559067 1104 24-25 0.6203849454777739 1104 26-27 0.6364787910993783 1104 28-29 0.6924766443538672 1104 30-31 0.6917653501434984 1104 32-33 0.682303851806104 1104 34-35 0.6418912862252242 1104 36-37 0.5571330906974197 1104 38-39 0.462942967848754 1104 40-41 0.4674216410819554 1104 42-43 0.495999016998816 1104 44-45 0.41191339951227945 1104 46-47 0.345203096588925 1104 48-49 0.34339537909472284 1104 50-51 0.26733489710205305 1104 52-53 0.22248673885952286 1104 54-55 0.35119603103395036 1104 56-57 0.2123614765057127 1104 58-59 0.34065452088223225 1104 60-61 0.024865197462858646 1104 62-63 0.1858849309532431 1104 64-65 0.2527502343575989 1104 66-67 0.3484079327437044 1104 68-69 0.2606427497553412 1104 70-71 0.039012779513143414 1104 72-73 0.12404314086831647 1104 74-75 0.19036897625579385 1104 76-77 0.1438405924405579 1104 78-79 0.14664813840077784 1104 80-81 0.22188883872166798 1104 82-83 0.18934257506839103 1104 84-85 0.1541092088831384 1104 86-87 0.2711733832877954 1104 88-89 0.31095307460197574 1104 90-91 0.2652701173746692 1104 92-93 0.1497330010960134 1104 94-95 0.11233723124139061 1104 96-97 0.10644902191978645 1104 98-99 0.05748300676616225 1104 100-101 0.03705508947807701 1104 102-103 0.008044768546056957 1104 104-105 0.09440784916715828 1104 106-107 0.1927741656924944 1104 108-109 0.2358306310625231 1104 110-111 0.22863861129934193 1104 112-113 0.2524201590715158 1104 114-115 0.03186129082405387 1104 116-117 -0.003898445721560506 1104 118-119 0.0755932285443528 1104 120-121 -0.02891967668263362 1104 122-123 -0.038742609967791 1104 124-125 -0.039380504901810554 1105 1 0.2938899310352916 1105 2 0.21200581114350214 1105 3 0.1406860130480716 1105 4 0.34138784658070165 1105 5 0.293346947612271 1105 6 0.40054899717976156 1105 7 0.4413765134852454 1105 8 0.4498202755601852 1105 9 0.4792011621043315 1105 10-11 0.5948319232537145 1105 12-13 0.624025088431587 1105 14-15 0.6841071519755104 1105 16-17 0.6301430043394092 1105 18-19 0.6205753607012099 1105 20-21 0.6775179072679336 1105 22-23 0.5989579976055026 1105 24-25 0.6330533093213901 1105 26-27 0.5962126747158223 1105 28-29 0.666470689963738 1105 30-31 0.6380047392198946 1105 32-33 0.7024402148211095 1105 34-35 0.6561337974582386 1105 36-37 0.541955089163082 1105 38-39 0.5642459394706876 1105 40-41 0.5980263020544214 1105 42-43 0.6080063110441145 1105 44-45 0.47750873262118887 1105 46-47 0.543950283170382 1105 48-49 0.4600358099715649 1105 50-51 0.47345657137957886 1105 52-53 0.510983310180535 1105 54-55 0.4450132025048532 1105 56-57 0.4114652835569075 1105 58-59 0.4395328037922255 1105 60-61 0.5127364962298415 1105 62-63 0.5289665163730817 1105 64-65 0.48643894134101373 1105 66-67 0.5039110567780725 1105 68-69 0.35032205722062315 1105 70-71 0.30992724062340926 1105 72-73 0.3363643630760649 1105 74-75 0.28527343456746834 1105 76-77 0.36731101134342836 1105 78-79 0.4979564803604468 1105 80-81 0.3667149802338976 1105 82-83 0.38627301019193183 1105 84-85 0.43794815763689954 1105 86-87 0.41448388780361256 1105 88-89 0.4423089819810002 1105 90-91 0.3995284857503947 1105 92-93 0.3864067145471104 1105 94-95 0.3755307239843759 1105 96-97 0.35493560065712515 1105 98-99 0.42377624343041376 1105 100-101 0.39407611881934557 1105 102-103 0.29544669228365805 1105 104-105 0.3074067103551208 1105 106-107 0.31835226565047847 1105 108-109 0.3235395127899636 1105 110-111 0.28768678916252455 1105 112-113 0.2756959539303203 1105 114-115 0.3837448222439832 1105 116-117 0.34162855866498987 1105 118-119 0.35256347777459496 1105 120-121 0.3057280749785285 1105 122-123 0.3303813580072692 1105 124-125 0.15636289780726997 1106 1 0.12607213388864125 1106 2 0.14585451912687475 1106 3 0.08566372244685283 1106 4 0.12512928550253122 1106 5 0.08997813733981985 1106 6 0.008160341767101897 1106 7 0.17323238321744583 1106 8 0.2342928496945902 1106 9 0.12574371041907284 1106 10-11 0.23830643961155573 1106 12-13 0.0725081429468517 1106 14-15 0.10707920497232948 1106 16-17 0.20201727760643706 1106 18-19 0.045891004753620734 1106 20-21 0.08078249002461035 1106 22-23 0.1057031710798455 1106 24-25 0.048379803979180735 1106 26-27 0.24205370286889405 1106 28-29 0.20001646580967503 1106 30-31 0.27475562032222456 1106 32-33 0.2533108682120826 1106 34-35 0.15021508036769404 1106 36-37 0.24211390442133052 1106 38-39 0.19631233073921095 1106 40-41 0.21911307691861737 1106 42-43 0.12979134623144972 1106 44-45 0.09488860538851895 1106 46-47 0.21920385931512243 1106 48-49 0.19719663032157797 1106 50-51 0.026837373200898185 1106 52-53 0.11583485218602618 1106 54-55 0.08041066850429957 1106 56-57 0.08804746219432502 1106 58-59 0.13694265834229924 1106 60-61 0.06326137389130793 1106 62-63 0.09929986105763788 1106 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2215 98-99 -0.664223237174884 2215 100-101 -0.5366050056569556 2215 102-103 -0.3933232370394819 2215 104-105 -0.5361524379010945 2215 106-107 -0.5798672852981177 2215 108-109 -0.47060334563871464 2215 110-111 -0.5409505489104873 2215 112-113 -0.4902438645833236 2215 114-115 -0.39334139992458717 2215 116-117 -0.37537008910862824 2215 118-119 -0.6035951880428279 2215 120-121 -0.5364934068900453 2215 122-123 -0.41425725499178867 2215 124-125 -0.25423172177184483 2216 1 -0.8170978155474451 2216 2 -0.9622051131192748 2216 3 -1.2786191821989306 2216 4 -0.8987588575287901 2216 5 -0.8751487879439281 2216 6 -0.8488419533325029 2216 7 -0.7558005147044184 2216 8 -1.054150068668001 2216 9 -1.080573752957953 2216 10-11 -1.0906979936960752 2216 12-13 -1.2352580740386756 2216 14-15 -1.4532509285199922 2216 16-17 -1.753211330227387 2216 18-19 -1.7200112170552018 2216 20-21 -1.7528635524974945 2216 22-23 -1.4179763902064835 2216 24-25 -1.4687454717017303 2216 26-27 -1.469085935115011 2216 28-29 -1.4845621290245603 2216 30-31 -1.5816759816752253 2216 32-33 -1.6025823995704584 2216 34-35 -1.2207631722187742 2216 36-37 -1.235703027765645 2216 38-39 -1.7185947222094526 2216 40-41 -1.4175009619635972 2216 42-43 -1.3818263347765978 2216 44-45 -1.3074000224377684 2216 46-47 -1.3018649981556152 2216 48-49 -1.4515068849462125 2216 50-51 -1.385749225344945 2216 52-53 -1.3033397916700977 2216 54-55 -1.2235205300576695 2216 56-57 -1.3523499889899853 2216 58-59 -1.52193891765598 2216 60-61 -1.581428658786976 2216 62-63 -1.5396269277304455 2216 64-65 -1.486653485485263 2216 66-67 -1.6327682216025359 2216 68-69 -1.5677321860436209 2216 70-71 -1.4977641490629132 2216 72-73 -1.3639444689096827 2216 74-75 -1.441892970921213 2216 76-77 -1.568128028289781 2216 78-79 -1.6163726708330692 2216 80-81 -1.6006425735160938 2216 82-83 -1.4899806852569846 2216 84-85 -1.457703695409883 2216 86-87 -1.583059749769145 2216 88-89 -1.6301088539509472 2216 90-91 -1.7402928158377442 2216 92-93 -1.651740976661511 2216 94-95 -1.6091241781507009 2216 96-97 -1.549183549175634 2216 98-99 -1.5246837667094404 2216 100-101 -1.504047528651835 2216 102-103 -1.6250514306367982 2216 104-105 -1.757753354434577 2216 106-107 -1.6384438350061963 2216 108-109 -1.6331471235668928 2216 110-111 -1.6330447341415741 2216 112-113 -1.7422210062872274 2216 114-115 -1.6797499585859939 2216 116-117 -1.7495047233106575 2216 118-119 -1.7280511491798514 2216 120-121 -1.5307734816641698 2216 122-123 -1.5531293541307036 2216 124-125 -1.470300259966173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46606.0 3 8003.0 4 5038.0 5 4180.0 6 4275.0 7 4782.0 8 5251.0 9 5826.0 10 6364.0 11 7009.0 12 7573.0 13 7410.0 14 7437.0 15 6819.0 16 6375.0 17 5885.0 18 5487.0 19 5378.0 20 5485.0 21 5732.0 22 6848.0 23 8660.0 24 12204.0 25 16760.0 26 19736.0 27 21336.0 28 25433.0 29 30477.0 30 36082.0 31 44046.0 32 55858.0 33 72795.0 34 101350.0 35 152948.0 36 290924.0 37 484214.0 38 177381.0 39 327.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11578397021853 18.510134619796016 12.58755060905531 24.786530800930148 2 15.64351191688735 19.837732444780723 39.302738410499664 25.21601722783226 3 18.58042025194165 24.45696360551839 29.21842217831463 27.74419396422533 4 12.440853869335502 16.1245883778508 37.38413760430962 34.050420148504074 5 13.311884677871424 38.383003444319556 34.82371801401827 13.481393863790753 6 32.94273933511894 36.61578602069727 17.20096099046985 13.240513653713942 7 28.98351920160257 32.01888907383244 21.311026037077433 17.68656568748756 8 26.603014385198342 35.22802267830767 19.843286422463212 18.32567651403078 9 26.567921438356883 15.751786364847925 19.61468759129695 38.065604605498244 10-11 24.947818776859908 26.445324582775733 28.02968997384319 20.577166666521173 12-13 25.457180228455535 23.762576184580197 27.84020625306448 22.94003733389979 14-15 22.531725572596716 25.52120843361469 24.936237498839688 27.010828494948903 16-17 22.898136258100713 28.66700944921711 25.872487801102316 22.56236649157986 18-19 23.004015321014716 27.731350901344854 26.76178429354473 22.5028494840957 20-21 23.292696127670816 27.12824464265137 26.88716249954897 22.69189673012884 22-23 23.367224516649802 26.91097300897664 26.7221519490122 22.999650525361353 24-25 23.122818330274097 27.080377397581557 26.743327975305743 23.05347629683861 26-27 22.795429652899912 27.377212514272877 26.61785468610145 23.209503146725762 28-29 22.98746688929129 27.420337155256526 26.634642552749437 22.95755340270275 30-31 22.956060766103114 27.08725255609708 26.641897906265722 23.314788771534083 32-33 22.696498236765997 27.227306891758467 26.610764238780206 23.465430632695323 34-35 23.513754876602 27.23343061075848 26.2634207491613 22.98939376347822 36-37 23.4647631501836 26.841892843102766 26.287447400575047 23.40589660613859 38-39 22.951854820992352 27.11831658463271 26.473344904898322 23.456483689476617 40-41 23.253706665844142 26.885632485096373 26.598952039222727 23.261708809836758 42-43 23.038452271624166 26.745373005425172 26.598366046164607 23.617808676786055 44-45 22.980106531104553 26.96947960966724 26.721324116739005 23.32908974248921 46-47 23.365944176591487 27.350682523096314 26.13153689983696 23.15183640047524 48-49 23.128725352006118 27.16869173726964 26.273647001037077 23.428935909687166 50-51 23.10175092271753 27.157255975985485 26.458947071921337 23.282046029375646 52-53 23.329302168041394 26.93369353870937 26.39836138368682 23.338642909562417 54-55 22.84913139999424 27.0053865742162 26.659628512323707 23.485853513465855 56-57 22.86920798722919 27.331980052296085 26.617230006756703 23.181581953718023 58-59 22.922896241711197 26.816174527813246 26.545613583800566 23.71531564667499 60-61 22.704879774844088 27.044723897674178 26.800729335682977 23.449666991798757 62-63 22.842975043080845 27.034024120618223 26.857306808374183 23.26569402792675 64-65 22.738805220956575 26.955552613315447 26.64270842578353 23.66293373994445 66-67 22.572064600611085 27.15166593918231 26.608671613560308 23.667597846646295 68-69 22.810600350939474 26.70399157279666 26.994084079976954 23.49132399628691 70-71 22.81441380831523 26.645362280294876 26.69497616496602 23.845247746423873 72-73 22.766165476983783 26.82705318508129 26.589717997142433 23.817063340792494 74-75 22.703681221886544 27.12041418620338 26.571050614545754 23.604853977364325 76-77 22.864257122537797 26.866519853015635 26.718173659908352 23.55104936453822 78-79 22.955261362164865 26.791211445315966 26.783063656058538 23.470463536460628 80-81 22.886241850463183 27.0102966787864 26.700246904958323 23.403214565792098 82-83 22.925934019695795 27.013293192545706 26.601865638742805 23.45890714901569 84-85 22.815916642684485 26.96914576967385 26.67466687074559 23.540270716896075 86-87 22.868371798512918 26.855157298296422 26.783893511125186 23.49257739206548 88-89 22.844404506205155 26.949843397934536 26.662276435019383 23.54347566084093 90-91 22.787008366745905 27.057764679488134 26.726097828044793 23.429129125721175 92-93 22.832642428848864 26.469420856566366 27.076685450965766 23.621251263619005 94-95 22.54893117016367 26.446780241415695 27.167704234639157 23.836584353781483 96-97 22.87422626654841 26.273095608895346 27.104851794689143 23.7478263298671 98-99 22.678067967247546 26.42552873259855 26.88910182749238 24.007301472661524 100-101 22.45312354049852 26.349201360790076 27.216674479773655 23.98100061893775 102-103 22.401071309785042 26.148764120666684 27.291162829135455 24.159001740412815 104-105 22.256345522568083 26.44774988455015 27.022452995832747 24.27345159704902 106-107 22.40834978245832 26.366703304346206 26.9568347605732 24.26811215262227 108-109 22.48391399691782 26.208952756968664 26.925402615663252 24.381730630450267 110-111 22.396105379401053 26.47835683326374 27.081115134866952 24.044422652468256 112-113 22.377095333776616 26.515631433680444 27.322876003381342 23.784397229161605 114-115 22.32300669928526 26.71260700611031 26.985007359261758 23.979378935342673 116-117 22.180917589449344 26.550417485565365 27.228806067522843 24.03985885746245 118-119 21.970256262161303 26.7704702894492 26.947264048888584 24.312009399500916 120-121 22.05718952060938 26.89320676547093 27.07116735745837 23.978436356461323 122-123 22.043193018346045 26.962069703049817 27.073551514869333 23.9211857637348 124-125 21.887284507781626 26.862531074557694 27.193537132186798 24.056647285473883 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 355.0 1 310.0 2 240.0 3 201.0 4 178.0 5 154.5 6 139.0 7 140.0 8 127.0 9 115.0 10 117.5 11 120.5 12 120.5 13 134.0 14 151.5 15 162.0 16 198.0 17 240.5 18 315.0 19 422.0 20 579.0 21 782.5 22 1151.5 23 1753.0 24 2552.0 25 3807.5 26 5632.0 27 7763.5 28 9881.0 29 12594.0 30 16006.5 31 19911.5 32 25377.5 33 29059.0 34 30818.0 35 34040.0 36 39621.5 37 45015.5 38 48131.5 39 50973.5 40 52703.0 41 54997.0 42 57543.5 43 59137.5 44 61271.5 45 64975.5 46 66431.0 47 65733.0 48 65021.0 49 63102.5 50 62162.5 51 60809.0 52 59777.5 53 61372.5 54 63758.5 55 61106.5 56 55266.0 57 50797.0 58 47251.5 59 41618.5 60 33748.0 61 27885.0 62 23565.5 63 18280.0 64 13246.0 65 10102.5 66 7494.0 67 5899.5 68 4514.0 69 2946.0 70 1994.5 71 1329.5 72 952.0 73 690.5 74 466.0 75 379.0 76 272.0 77 144.0 78 78.5 79 49.5 80 35.0 81 26.0 82 23.5 83 19.0 84 14.0 85 11.5 86 11.5 87 8.5 88 8.5 89 10.5 90 7.5 91 3.5 92 3.5 93 4.5 94 6.0 95 5.5 96 3.5 97 2.0 98 1.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04166923704558126 2 0.00838040521587109 3 0.0022696930792984204 4 0.005354147776806531 5 0.022871522568314852 6 0.028109275828234283 7 0.0030262574390645604 8 0.0 9 0.0 10-11 2.0369040455242232E-4 12-13 9.602547643185624E-4 14-15 2.0369040455242232E-4 16-17 2.0369040455242232E-4 18-19 2.909862922177462E-5 20-21 0.0 22-23 2.909862922177462E-5 24-25 0.0 26-27 0.0 28-29 3.200849214395208E-4 30-31 2.909862922177462E-5 32-33 1.454931461088731E-4 34-35 7.274657305443655E-4 36-37 2.909862922177462E-5 38-39 8.729588766532386E-5 40-41 2.618876629959716E-4 42-43 4.6557806754839393E-4 44-45 9.602547643185624E-4 46-47 2.909862922177462E-5 48-49 0.0 50-51 0.0 52-53 8.729588766532387E-4 54-55 0.0023569889669637443 56-57 5.819725844354924E-5 58-59 6.983671013225909E-4 60-61 0.0010475506519838863 62-63 7.565643597661401E-4 64-65 0.001542227348754055 66-67 0.0025606793715161665 68-69 0.0028516656637339126 70-71 0.001454931461088731 72-73 0.0028516656637339126 74-75 0.0019205095286371249 76-77 9.311561350967879E-4 78-79 0.002124199933189547 80-81 0.0012512410565363088 82-83 4.946766967701686E-4 84-85 2.618876629959716E-4 86-87 0.0017459177533064774 88-89 0.00497586559692346 90-91 2.0369040455242232E-4 92-93 8.729588766532387E-4 94-95 7.565643597661401E-4 96-97 1.1639451688709848E-4 98-99 6.11071213657267E-4 100-101 0.0016004246071976043 102-103 0.0013676355734234071 104-105 9.020575058750132E-4 106-107 0.006838177867117035 108-109 0.0017459177533064774 110-111 0.001542227348754055 112-113 0.0024151862254072932 114-115 0.0029680601806210114 116-117 0.0018041150117500263 118-119 0.04399712738332323 120-121 0.012628805082250184 122-123 0.004452090270931517 124-125 0.0027643697760685888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1718294.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.82531627625848 #Duplication Level Percentage of deduplicated Percentage of total 1 86.20639095765821 62.780076865262494 2 8.837773494044951 12.87227299763515 3 2.3804769875756526 5.200769685256558 4 0.9672097409501528 2.817494211606917 5 0.4603706461031604 1.676331895338406 6 0.25582492882559676 1.117831881184524 7 0.1555403309733187 0.7929091657792101 8 0.1104323807391493 0.6433818443574995 9 0.08865813820852059 0.5810901259949773 >10 0.46935884157209495 6.560408826952004 >50 0.04504272023414699 2.23860734729579 >100 0.022759418808996346 2.653819738521797 >500 1.614143060130086E-4 0.06500541481461299 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.454931461088731E-4 14-15 0.0 0.0 0.0 0.0 3.200849214395208E-4 16-17 0.0 0.0 0.0 0.0 4.364794383266193E-4 18-19 0.0 0.0 0.0 0.0 5.819725844354925E-4 20-21 0.0 0.0 0.0 0.0 6.11071213657267E-4 22-23 0.0 0.0 0.0 2.909862922177462E-5 6.401698428790416E-4 24-25 0.0 0.0 0.0 2.0369040455242235E-4 6.401698428790416E-4 26-27 0.0 0.0 0.0 4.946766967701686E-4 6.983671013225909E-4 28-29 0.0 0.0 0.0 0.0027352711468468143 6.983671013225909E-4 30-31 0.0 0.0 0.0 0.010359112002951764 6.983671013225909E-4 32-33 0.0 0.0 0.0 0.027789190906794763 6.983671013225909E-4 34-35 0.0 0.0 0.0 0.055432888667480654 6.983671013225909E-4 36-37 0.0 0.0 0.0 0.10204889268076359 6.983671013225909E-4 38-39 0.0 0.0 0.0 0.17133272885780898 7.565643597661401E-4 40-41 0.0 0.0 0.0 0.24760023604808024 7.565643597661401E-4 42-43 0.0 0.0 0.0 0.32325667202469427 7.565643597661401E-4 44-45 0.0 0.0 0.0 0.41398619793818753 7.856629889879147E-4 46-47 0.0 0.0 0.0 0.5287511915888666 8.43860247431464E-4 48-49 0.0 0.0 0.0 0.6485793467241345 8.729588766532386E-4 50-51 0.0 0.0 0.0 0.7799596576604468 8.729588766532386E-4 52-53 0.0 0.0 0.0 0.9083428097869166 8.729588766532386E-4 54-55 0.0 0.0 0.0 1.0455719451968055 8.729588766532386E-4 56-57 0.0 0.0 0.0 1.2159444192902962 9.020575058750133E-4 58-59 0.0 0.0 0.0 1.3978108519263874 9.311561350967879E-4 60-61 0.0 0.0 0.0 1.5962926018481123 9.893533935403371E-4 62-63 0.0 0.0 0.0 1.7906132477911232 0.0010475506519838863 64-65 0.0 0.0 0.0 1.9891240963420698 0.0010475506519838863 66-67 0.0 0.0 0.0 2.218974168564867 0.0011057479104274355 68-69 0.0 0.0 0.0 2.4760314591100245 0.001163945168870985 70-71 0.0 0.0 0.0 2.759102924179448 0.001163945168870985 72-73 0.0 0.0 0.0 3.047906819205561 0.0012512410565363088 74-75 0.0 0.0 0.0 3.333626259534166 0.0013385369442016327 76-77 0.0 0.0 0.0 3.6436430552629524 0.0013967342026451818 78-79 0.0 0.0 0.0 3.996434835947748 0.0014840300903105055 80-81 0.0 0.0 0.0 4.37817975270821 0.0015131287195322802 82-83 0.0 0.0 0.0 4.774735871742553 0.001687720494862928 84-85 0.0 0.0 0.0 5.1651812786403255 0.001687720494862928 86-87 0.0 0.0 0.0 5.5721547069360655 0.0017750163825282518 88-89 0.0 0.0 0.0 6.01116572600498 0.0018623122701935757 90-91 0.0 0.0 0.0 6.502321488639313 0.0018623122701935757 92-93 0.0 0.0 0.0 7.003312587950607 0.0019205095286371249 94-95 0.0 0.0 0.0 7.5120148240056706 0.0019496081578588996 96-97 0.0 0.0 0.0 8.010445243945448 0.0020078054163024488 98-99 0.0 0.0 0.0 8.53107791798144 0.0020951013039677726 100-101 0.0 0.0 0.0 9.115727576305336 0.0020951013039677726 102-103 0.0 0.0 0.0 9.726536902299607 0.002153298562411322 104-105 0.0 0.0 0.0 10.336531466675668 0.002211495820854871 106-107 0.0 0.0 0.0 10.947573581703713 0.002211495820854871 108-109 0.0 0.0 0.0 11.574008871590078 0.002211495820854871 110-111 0.0 0.0 0.0 12.259456181538201 0.002211495820854871 112-113 0.0 0.0 0.0 12.96911355099884 0.002211495820854871 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1580 0.0 43.71423 1 GTATCAA 2340 0.0 42.4935 1 ATCAACG 2375 0.0 41.10919 3 TCAACGC 2465 0.0 39.84976 4 CAACGCA 2495 0.0 39.13085 5 AACGCAG 2575 0.0 38.142994 6 GTACATG 5675 0.0 34.83326 1 TATCAAC 2860 0.0 34.13788 2 TACATGG 6015 0.0 31.770796 2 ACATGGG 6115 0.0 31.251242 3 ACGCAGA 3240 0.0 30.113882 7 CGCAGAG 3355 0.0 28.726173 8 CATGGGG 3615 0.0 26.020012 4 GTATAGG 550 0.0 25.981873 1 CATGGGA 3030 0.0 24.756348 4 TAGCCCT 1110 0.0 23.062382 4 GTGTAAG 1165 0.0 22.998976 1 TAAGGTA 675 0.0 22.049305 4 GTATAGA 655 0.0 21.816841 1 TAAGGTG 930 0.0 21.764162 5 >>END_MODULE