FastQCFastQC Report
Thu 26 May 2016
SRR1364363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718294
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22880.13315532731884067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.12401835774320344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22600.069.554821
GTATCAA39150.053.687691
ATCAACG49000.041.5538443
TCAACGC48900.041.5170674
CAACGCA49350.041.1384935
TATCAAC50900.040.1196822
AACGCAG50750.039.9896666
GTACATG55500.033.4729041
ACGCAGA61200.033.1517147
CGCAGAG63350.031.9326788
ACATGGG58700.030.2245143
TACATGG60000.029.867332
GCAGAGT73950.026.7922449
CTAACGG1401.056448E-425.4991849
GAGTACT42500.024.84920312-13
CAGAGTA70750.024.64019810-11
GTACTTT43050.024.32442514-15
CATGGGG34800.023.7802684
AGAGTAC68050.023.38830410-11
CATGGGA31850.022.0573714
Produced by FastQC (version 0.11.5)