FastQCFastQC Report
Thu 26 May 2016
SRR1364362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504711
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.049.507641
GTATCAA20450.040.4678341
CAACGCA21300.038.000475
ATCAACG21750.037.487893
TCAACGC21800.037.4019054
TAGATCG650.00193662136.6248865
GTACATG54850.035.6029241
AACGCAG22800.035.2358936
ACATGGG56400.033.873153
TACATGG58100.032.8820232
TATCAAC25350.032.1641652
CATGGGG30950.029.8057944
ACGCAGA29450.027.2721487
CGCAGAG30300.026.5070888
CATGGGA30700.026.1712864
TAAGGTG7850.025.019235
GTAAGGT7950.023.2072774
ACACGGC3950.022.592548
GTGTAGC10200.021.5968321
GGTAAGG8750.021.085473
Produced by FastQC (version 0.11.5)