FastQCFastQC Report
Thu 26 May 2016
SRR1364362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504711
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20020.1330488047206407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.13078923461049996No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19850.071.706011
GTATCAA36600.049.4664461
TATTACG705.4305914E-542.529382
ATCAACG42400.041.8473053
CAACGCA43350.040.7928855
TCAACGC43450.040.6990054
AACGCAG45300.039.013556
TATCAAC46900.037.7051542
ACGCAGA54200.032.6029367
CGCAGAG56000.031.554988
GTACATG55700.031.5416971
TACATGG57750.030.3118522
ACATGGG56750.029.586963
TGGTATC10250.026.140012
GCAGAGT66600.025.8180319
GTGGTAT11650.025.5600851
GAGTACA44250.024.2257631
CAGAGTA63400.024.16503510-11
GAGTACT40400.024.07885712-13
GTACCGA1250.001632879523.8022086
Produced by FastQC (version 0.11.5)