FastQCFastQC Report
Thu 26 May 2016
SRR1364361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144655
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30850.049.417191
GGTATCA21450.048.307571
TCAACGC35300.043.0007824
ATCAACG35600.042.3039933
CAACGCA37250.040.7497335
AACGCAG38100.039.8331836
TATCAAC39800.037.8397522
GTACATG92000.035.9251371
TACATGG94650.034.275732
ACATGGG95650.033.108353
ACGCAGA48350.030.8855367
CGCAGAG48800.030.4788178
CATGGGG51300.030.2854484
GAGTACA73650.024.7424051
ATGGGGA31200.024.6119465
CATGGGA53300.024.1233374
GCAGAGT59550.023.9776949
AGTACAT74700.023.3484382
CCTACGC5350.022.79648296-97
TCGCGGA5250.022.66568682-83
Produced by FastQC (version 0.11.5)