##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364361_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2144655 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.669828480571468 31.0 31.0 34.0 27.0 34.0 2 30.649673257470315 31.0 31.0 34.0 27.0 34.0 3 30.819615742392134 31.0 31.0 34.0 27.0 34.0 4 34.23894519165087 37.0 35.0 37.0 30.0 37.0 5 34.33521055834155 37.0 35.0 37.0 30.0 37.0 6 34.381910377193535 37.0 35.0 37.0 30.0 37.0 7 34.313943268264595 37.0 35.0 37.0 30.0 37.0 8 34.26432176737051 37.0 35.0 37.0 30.0 37.0 9 35.78733409336233 39.0 37.0 39.0 30.0 39.0 10-11 35.687764465613355 39.0 37.0 39.0 30.0 39.0 12-13 35.68620081085302 39.0 37.0 39.0 30.0 39.0 14-15 36.92096887378156 40.0 37.0 41.0 30.0 41.0 16-17 36.910513112831666 40.0 37.0 41.0 30.0 41.0 18-19 36.89107502139039 40.0 37.0 41.0 30.0 41.0 20-21 36.90398968598679 40.0 37.0 41.0 30.0 41.0 22-23 36.843037691376935 40.0 37.0 41.0 30.0 41.0 24-25 36.8252611725429 40.0 37.0 41.0 30.0 41.0 26-27 36.76311388078735 40.0 37.0 41.0 30.0 41.0 28-29 36.754496643982364 40.0 37.0 41.0 30.0 41.0 30-31 36.64893094693552 40.0 37.0 41.0 30.0 41.0 32-33 36.60332267893904 40.0 37.0 41.0 30.0 41.0 34-35 36.587999235308246 40.0 37.0 41.0 30.0 41.0 36-37 36.5172808680184 40.0 37.0 41.0 30.0 41.0 38-39 36.41902497138234 40.0 36.0 41.0 29.0 41.0 40-41 36.352693556772536 40.0 36.0 41.0 29.0 41.0 42-43 36.20562188323996 40.0 36.0 41.0 28.5 41.0 44-45 36.06040248897841 40.0 35.5 41.0 28.0 41.0 46-47 35.93506484725982 40.0 35.0 41.0 28.0 41.0 48-49 35.77706274435749 39.5 35.0 41.0 28.0 41.0 50-51 35.64310296994155 39.0 35.0 41.0 27.0 41.0 52-53 35.47368341294987 39.0 35.0 41.0 27.0 41.0 54-55 35.28176746376457 39.0 35.0 41.0 27.0 41.0 56-57 35.08500901077329 39.0 34.0 41.0 26.0 41.0 58-59 34.86233986352117 38.0 34.0 40.0 26.0 41.0 60-61 34.60929100484694 38.0 34.0 40.0 26.0 41.0 62-63 34.341163497159215 37.5 34.0 40.0 26.0 41.0 64-65 34.00301377144576 37.0 33.5 40.0 25.0 41.0 66-67 33.69103398914977 36.5 33.0 39.5 24.5 41.0 68-69 33.3746562967004 36.0 33.0 39.0 23.5 41.0 70-71 33.022409198682304 35.5 33.0 39.0 23.0 40.5 72-73 32.66788900778913 35.0 32.0 38.5 22.5 40.0 74-75 32.31052593540686 35.0 32.0 37.0 22.0 39.5 76-77 32.08890311029047 35.0 32.0 37.0 22.5 39.0 78-79 31.9367508993288 35.0 32.0 36.5 22.0 39.0 80-81 31.67991308625397 35.0 32.0 36.0 22.0 38.0 82-83 31.398440075443368 35.0 32.0 36.0 20.5 37.0 84-85 31.157054398026723 35.0 32.0 35.0 20.0 37.0 86-87 30.957837507664404 35.0 32.0 35.0 20.0 36.0 88-89 30.70547477333184 35.0 31.5 35.0 19.0 36.0 90-91 30.58377944238118 35.0 31.0 35.0 18.5 36.0 92-93 30.44268238947523 35.0 31.0 35.0 18.0 35.5 94-95 30.345204473446778 35.0 31.0 35.0 17.0 35.0 96-97 30.253758296788995 35.0 31.0 35.0 16.0 35.0 98-99 30.123669308117158 34.5 31.0 35.0 12.0 35.0 100-101 30.03983857543521 34.0 31.0 35.0 10.0 35.0 102-103 29.006666107136112 33.5 29.5 34.5 7.5 35.0 104-105 29.732304496527412 34.0 30.5 35.0 7.0 35.0 106-107 30.10124495548235 34.5 31.5 35.0 7.5 35.0 108-109 30.151999738885742 35.0 32.0 35.0 7.0 35.0 110-111 30.099170729091625 35.0 32.0 35.0 3.5 35.0 112-113 30.0163658024251 35.0 32.0 35.0 2.0 35.0 114-115 29.923599599935656 35.0 31.5 35.0 2.0 35.0 116-117 29.799746579286644 35.0 31.0 35.0 2.0 35.0 118-119 29.625555392359146 35.0 31.0 35.0 2.0 35.0 120-121 29.447830769983984 35.0 31.0 35.0 2.0 35.0 122-123 29.265505407629668 35.0 31.0 35.0 2.0 35.0 124-125 28.4133555280453 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36155104131529114 1101 2 0.2861474763622738 1101 3 0.46182948690344006 1101 4 0.4520635004429536 1101 5 0.49426548964403594 1101 6 0.3323891607440075 1101 7 0.382860238478834 1101 8 0.21436272777048515 1101 9 0.4641652548104034 1101 10-11 0.5734801219981946 1101 12-13 0.5973569940253682 1101 14-15 0.6981317229940132 1101 16-17 0.5999501284872011 1101 18-19 0.4343226988496127 1101 20-21 0.5996170158558982 1101 22-23 0.7177566799744071 1101 24-25 0.7541160229422914 1101 26-27 0.8392321230538471 1101 28-29 0.7329541874687209 1101 30-31 0.7648542951410704 1101 32-33 0.7066707980629232 1101 34-35 0.6736342137442008 1101 36-37 0.6264029235634894 1101 38-39 0.7275700362263535 1101 40-41 0.6089128145643627 1101 42-43 0.6976472336297022 1101 44-45 0.7005726597709696 1101 46-47 0.6825177667594815 1101 48-49 0.49389140035337675 1101 50-51 0.5433890701769073 1101 52-53 0.4695668527054764 1101 54-55 0.4433343576822111 1101 56-57 0.3390200806663515 1101 58-59 0.41062001726844954 1101 60-61 0.432357494661062 1101 62-63 0.4299255118493832 1101 64-65 0.46331620120701444 1101 66-67 0.500069087529269 1101 68-69 0.4052004971311618 1101 70-71 0.42863449009769994 1101 72-73 0.4408807622008055 1101 74-75 0.3730094097697574 1101 76-77 0.46774175353927916 1101 78-79 0.4278548782327718 1101 80-81 0.4365595174153256 1101 82-83 0.5179360132279349 1101 84-85 0.4677482879408359 1101 86-87 0.42538955976865367 1101 88-89 0.5189101272497574 1101 90-91 0.5070088150219689 1101 92-93 0.41935989184395694 1101 94-95 0.5360256821225917 1101 96-97 0.4392847354124285 1101 98-99 0.3631344919095554 1101 100-101 0.34792908448706683 1101 102-103 0.16019880961238897 1101 104-105 0.3826878053822931 1101 106-107 0.43236211554416926 1101 108-109 0.40408831385297717 1101 110-111 0.3997504344364309 1101 112-113 0.5400031236646967 1101 114-115 0.46182913629610667 1101 116-117 0.45842624980891244 1101 118-119 0.49827539885346894 1101 120-121 0.4280491325456488 1101 122-123 0.32046356270441123 1101 124-125 0.17241540533702704 1102 1 0.31642327337061715 1102 2 0.30954670467254886 1102 3 0.3315666938731816 1102 4 0.4263244626944811 1102 5 0.38414816930208673 1102 6 0.41405426224605435 1102 7 0.3563957958921975 1102 8 0.2959089729731943 1102 9 0.43003597255607673 1102 10-11 0.42533502247537314 1102 12-13 0.4162417446487723 1102 14-15 0.4115761950421586 1102 16-17 0.5614961984464486 1102 18-19 0.5082070938318779 1102 20-21 0.554742680879059 1102 22-23 0.5619378239843229 1102 24-25 0.5673691703663621 1102 26-27 0.5246735664826758 1102 28-29 0.44175048137880424 1102 30-31 0.6378004160240067 1102 32-33 0.6320318253606985 1102 34-35 0.47065672428200145 1102 36-37 0.30010078734571977 1102 38-39 0.4286345371587572 1102 40-41 0.33772222080859393 1102 42-43 0.259733620408511 1102 44-45 0.2401900002143833 1102 46-47 0.3227019167101517 1102 48-49 0.38476858664439817 1102 50-51 0.41259003216041634 1102 52-53 0.3989323476806348 1102 54-55 0.2043489245370793 1102 56-57 0.3317077380633222 1102 58-59 0.352759162637021 1102 60-61 0.209725935788029 1102 62-63 0.29711962696784155 1102 64-65 0.23344662357467882 1102 66-67 0.1954208063068137 1102 68-69 0.2758216011855694 1102 70-71 0.20820733931926583 1102 72-73 0.23800542936708524 1102 74-75 0.15853713483132736 1102 76-77 0.14569385847093486 1102 78-79 0.3488586315353359 1102 80-81 0.2576070815827798 1102 82-83 0.17225731077174444 1102 84-85 0.18463650013798727 1102 86-87 0.14889243943103025 1102 88-89 0.180626698786714 1102 90-91 0.15628944062050465 1102 92-93 0.19160395650260753 1102 94-95 0.11822780174377812 1102 96-97 0.08949085264617906 1102 98-99 0.008873550274902442 1102 100-101 -0.005496203556482726 1102 102-103 -0.1691211736054754 1102 104-105 -0.023149863508589164 1102 106-107 -0.018235825448261522 1102 108-109 0.17480281236289485 1102 110-111 0.11841365671008575 1102 112-113 -0.007336783703671301 1102 114-115 -0.10089791995395103 1102 116-117 -0.11695353884747561 1102 118-119 -0.12124785646903646 1102 120-121 -0.14220772282386207 1102 122-123 -0.18764459137376122 1102 124-125 -0.13376893635061293 1103 1 0.4298019461297713 1103 2 0.3673385329494714 1103 3 0.3921904651924528 1103 4 0.4672734027082939 1103 5 0.4031641620761164 1103 6 0.34216647732495176 1103 7 0.2966646445187351 1103 8 0.3827747080563455 1103 9 0.42481308323031897 1103 10-11 0.61703312565799 1103 12-13 0.5237971349043349 1103 14-15 0.6212568708829806 1103 16-17 0.5202895675554231 1103 18-19 0.5787028166946371 1103 20-21 0.5820765101123655 1103 22-23 0.6565077193140141 1103 24-25 0.7157247306447729 1103 26-27 0.7431449548254889 1103 28-29 0.6705427878449797 1103 30-31 0.7902737171238456 1103 32-33 0.7646018269546602 1103 34-35 0.6655719785192886 1103 36-37 0.6409779989710316 1103 38-39 0.5192965636185534 1103 40-41 0.5760517463385924 1103 42-43 0.5952312028961586 1103 44-45 0.4658023481236313 1103 46-47 0.46553704768087556 1103 48-49 0.5255554736323305 1103 50-51 0.450202273805381 1103 52-53 0.5728364973007345 1103 54-55 0.5234738381973543 1103 56-57 0.3869253141751088 1103 58-59 0.3825344136863791 1103 60-61 0.34044693892282396 1103 62-63 0.36773224053779074 1103 64-65 0.42369421934536433 1103 66-67 0.3643385046225305 1103 68-69 0.30290039614864384 1103 70-71 0.32047289375314136 1103 72-73 0.3928271710386255 1103 74-75 0.2911788648137943 1103 76-77 0.2568941135052043 1103 78-79 0.3143811861286139 1103 80-81 0.32149847948937804 1103 82-83 0.3642263878664842 1103 84-85 0.3080677737153721 1103 86-87 0.3061130972069215 1103 88-89 0.36557702023615946 1103 90-91 0.3564382106114721 1103 92-93 0.2713367018402657 1103 94-95 0.21914964436637874 1103 96-97 0.22552506974410136 1103 98-99 0.2898894367846552 1103 100-101 0.21546708213503152 1103 102-103 0.028689782241720252 1103 104-105 0.19507977359889495 1103 106-107 0.17692914984529295 1103 108-109 0.14491864042728864 1103 110-111 0.24503285866534696 1103 112-113 0.2220479461193996 1103 114-115 0.12927050892867697 1103 116-117 0.15763070215990993 1103 118-119 0.026836729897258493 1103 120-121 -0.12181560763338695 1103 122-123 -0.1536214257608819 1103 124-125 -0.2668861989827782 1104 1 0.3813814271111866 1104 2 0.33321294698367154 1104 3 0.440810029705883 1104 4 0.5369706364325282 1104 5 0.4192731488031569 1104 6 0.45296744441022696 1104 7 0.5141007875836152 1104 8 0.49629316798841927 1104 9 0.6100565994584457 1104 10-11 0.6303879974036377 1104 12-13 0.5385828791094411 1104 14-15 0.6966597027151664 1104 16-17 0.7226627843812921 1104 18-19 0.6953844785567824 1104 20-21 0.7541444912310027 1104 22-23 0.7354149073630794 1104 24-25 0.7097416243515866 1104 26-27 0.6781795711294194 1104 28-29 0.6284037569896697 1104 30-31 0.6144355198647276 1104 32-33 0.6542364582615576 1104 34-35 0.5646199427991974 1104 36-37 0.5833684924985576 1104 38-39 0.5010934990075171 1104 40-41 0.4916842259785952 1104 42-43 0.5602404772420755 1104 44-45 0.49185512965861733 1104 46-47 0.4839961410826987 1104 48-49 0.4781619585457406 1104 50-51 0.40757341025172167 1104 52-53 0.2843836986195001 1104 54-55 0.27718648862882134 1104 56-57 0.3657879592716782 1104 58-59 0.34562619779470793 1104 60-61 0.16415422649464517 1104 62-63 0.35006317883321714 1104 64-65 0.37172275106334496 1104 66-67 0.3237444060046215 1104 68-69 0.20716487132690276 1104 70-71 0.16987433618067627 1104 72-73 0.11411339072576965 1104 74-75 0.15984797637292303 1104 76-77 0.22654904074317272 1104 78-79 0.3292315082962318 1104 80-81 0.2808763318574421 1104 82-83 0.21375572820678812 1104 84-85 0.1899174990260697 1104 86-87 0.20345263424908921 1104 88-89 0.15911400556354494 1104 90-91 0.1177320156465953 1104 92-93 0.1664409840673926 1104 94-95 0.14906592132103924 1104 96-97 0.07837630793101624 1104 98-99 0.12172450858220785 1104 100-101 0.14193225872500648 1104 102-103 -0.08831243978067249 1104 104-105 0.11628524753637848 1104 106-107 0.11990536456096024 1104 108-109 0.01939706621598347 1104 110-111 0.009580489106074452 1104 112-113 -0.04876486491830789 1104 114-115 -0.06374589228963501 1104 116-117 -0.06580294444953694 1104 118-119 -0.04583738016390626 1104 120-121 -0.08853000111073328 1104 122-123 -0.1632725696434747 1104 124-125 -0.30991851172648666 1105 1 0.18669757996991265 1105 2 0.2111145799296068 1105 3 0.20340171866049772 1105 4 0.25224688960887676 1105 5 0.2577175904860596 1105 6 0.3205371353798441 1105 7 0.348377970018916 1105 8 0.36151591628443214 1105 9 0.4126562232443476 1105 10-11 0.3914799388092334 1105 12-13 0.4505112226121568 1105 14-15 0.5934043967569949 1105 16-17 0.5378323039511912 1105 18-19 0.4903964889641159 1105 20-21 0.5489108624586763 1105 22-23 0.5416446704365612 1105 24-25 0.5155457877446779 1105 26-27 0.5510566700740966 1105 28-29 0.5715191171937306 1105 30-31 0.5690095312671346 1105 32-33 0.5935401538033744 1105 34-35 0.5181868197572967 1105 36-37 0.4193420903139966 1105 38-39 0.3888605573989423 1105 40-41 0.5003403720899513 1105 42-43 0.49949018463366457 1105 44-45 0.48205050416746786 1105 46-47 0.5368571678517071 1105 48-49 0.44461957646704064 1105 50-51 0.5130738500273253 1105 52-53 0.5629993283040591 1105 54-55 0.5013503511795037 1105 56-57 0.46721181043560733 1105 58-59 0.4602669280569316 1105 60-61 0.3194423727127784 1105 62-63 0.43192302228138857 1105 64-65 0.4862130693264817 1105 66-67 0.28339979315212815 1105 68-69 0.24279387470012637 1105 70-71 0.24720644707132777 1105 72-73 0.31585334642142726 1105 74-75 0.21831369113598953 1105 76-77 0.3157243475850393 1105 78-79 0.3836667982282478 1105 80-81 0.3567000423668105 1105 82-83 0.29851588136191864 1105 84-85 0.2700704332337054 1105 86-87 0.24550070441435778 1105 88-89 0.4099088924760892 1105 90-91 0.38146371525155587 1105 92-93 0.35765964804247474 1105 94-95 0.2757575155087295 1105 96-97 0.35432244681173586 1105 98-99 0.3643193641052207 1105 100-101 0.2548190414777025 1105 102-103 0.15562033691780286 1105 104-105 0.24380594874717332 1105 106-107 0.35613518746786 1105 108-109 0.3464505605104087 1105 110-111 0.3795512489766324 1105 112-113 0.2794686823885222 1105 114-115 0.22589146658980397 1105 116-117 0.1574892833279904 1105 118-119 0.176002874744853 1105 120-121 0.05348276733993629 1105 122-123 0.09541057054299529 1105 124-125 0.009780397326260015 1106 1 0.2739648163561661 1106 2 0.3255452621114472 1106 3 0.3145777627554729 1106 4 0.34261985482142165 1106 5 0.3224093283444489 1106 6 0.2060535964486192 1106 7 0.26245732667145916 1106 8 0.2245604557595584 1106 9 0.43062686777620485 1106 10-11 0.4469831485330218 1106 12-13 0.3142166107957465 1106 14-15 0.41866416352775104 1106 16-17 0.4561767543873074 1106 18-19 0.33913338450793873 1106 20-21 0.22801507525307585 1106 22-23 0.35011159711384465 1106 24-25 0.3399327678918169 1106 26-27 0.4580646947319096 1106 28-29 0.3413898411036058 1106 30-31 0.4710847392686617 1106 32-33 0.40645410738139276 1106 34-35 0.4596738408242942 1106 36-37 0.4633889944087244 1106 38-39 0.40361581160600224 1106 40-41 0.4147854993061131 1106 42-43 0.3658423118391454 1106 44-45 0.3065103597449905 1106 46-47 0.27567719898052445 1106 48-49 0.2694677294302039 1106 50-51 0.28682921988755083 1106 52-53 0.26125514846849285 1106 54-55 0.3071660799175575 1106 56-57 0.1750830536235597 1106 58-59 0.25076177034362956 1106 60-61 0.1619300027006716 1106 62-63 0.14704056352289285 1106 64-65 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-1.5009648750059803 2216 94-95 -1.4426234230404162 2216 96-97 -1.4417707587689783 2216 98-99 -1.603490920326891 2216 100-101 -1.52231117922911 2216 102-103 -1.5270516043632512 2216 104-105 -1.6760544667286403 2216 106-107 -1.4238885207601548 2216 108-109 -1.2879680749207374 2216 110-111 -1.3314078327892176 2216 112-113 -1.3353482050826706 2216 114-115 -1.3627358234549156 2216 116-117 -1.5246829108911655 2216 118-119 -1.5168752410683766 2216 120-121 -1.3749113222139187 2216 122-123 -1.3738449475715022 2216 124-125 -1.3060628991922556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49041.0 3 8845.0 4 5730.0 5 4734.0 6 4972.0 7 5505.0 8 6122.0 9 6753.0 10 7269.0 11 8046.0 12 8559.0 13 8516.0 14 8483.0 15 8285.0 16 7577.0 17 6883.0 18 6612.0 19 6683.0 20 6734.0 21 7096.0 22 8315.0 23 10691.0 24 14628.0 25 19888.0 26 23532.0 27 26081.0 28 30441.0 29 36440.0 30 43759.0 31 53910.0 32 67634.0 33 89236.0 34 123741.0 35 188007.0 36 358826.0 37 628086.0 38 238647.0 39 348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.91362535691278 18.604433112176856 12.692357389989695 24.789584140920663 2 15.305442967378754 20.21704907269414 39.3819608559976 25.095547103929512 3 18.086382709469607 25.017823678704705 29.73959477184526 27.156198839980433 4 12.214227759799304 16.215365532934804 37.52930698424837 34.041099723017524 5 13.14801739021749 38.49768257909499 34.910553802501084 13.443746228186443 6 32.29086455383832 36.8619690430894 17.4773573896833 13.369809013388977 7 28.41648104515674 32.36165116336542 21.72244948584746 17.499418305630382 8 26.481101380455772 35.20731082962566 20.239441886663304 18.072145903255258 9 26.800814116955873 15.511445896892507 19.483320161051545 38.204419825100075 10-11 24.693464325805696 26.56418697201528 28.157708533200598 20.584640168978424 12-13 25.210898456333343 24.04662054224637 27.97227871981886 22.770202281601428 14-15 22.26959855416833 25.520108661188225 25.210104408742133 27.000188375901313 16-17 22.893353843255763 28.751061360509134 25.84765460892741 22.50793018730769 18-19 22.84356291421299 27.787995688816075 26.929465795073288 22.43897560189765 20-21 23.341253027586976 27.219944853562595 26.841049148498815 22.59775297035162 22-23 23.396746796104733 26.97018867836552 26.56823591673253 23.06482860879722 24-25 23.109824190837223 27.202860133681174 26.634190580769403 23.053125094712204 26-27 22.831170629661155 27.55330872796002 26.522116509729894 23.093404132648935 28-29 22.92424268563976 27.463069748877555 26.66655320609274 22.94613435938994 30-31 22.982713737573153 27.101029491511675 26.629507551578325 23.28674921933685 32-33 22.771078298232464 27.245952432853343 26.648139966731204 23.33482930218299 34-35 23.40183364321476 27.181120352507126 26.268891595899074 23.14815440837904 36-37 23.375895804050213 26.939458462132425 26.267554425509225 23.417091308308134 38-39 22.917167913488996 27.168417067197847 26.44228527518684 23.47212974412632 40-41 23.164976021672583 26.738830902084025 26.72349036786974 23.372702708373648 42-43 23.13611169784435 26.833137263404677 26.56132046219348 23.469430576557496 44-45 22.846945950533147 26.951130918742084 26.70575036789597 23.4961727628288 46-47 23.163270159195843 27.166316804482214 26.310561050873478 23.35985198544847 48-49 23.098120676752202 27.17518668503792 26.268210970995337 23.45848166721454 50-51 22.953948304039578 27.011034408797684 26.53056085943893 23.50445642772381 52-53 23.28598113061505 26.90064710221492 26.386667586790868 23.42670418037916 54-55 22.824033770415824 26.86287739572759 26.591894335491155 23.721194498365428 56-57 22.889361847962853 27.06880155698811 26.661509656454996 23.38032693859404 58-59 23.025958717470004 26.728754902929914 26.465866468715436 23.779419910884645 60-61 22.787124119311954 26.80970610315066 26.942712729002206 23.46045704853518 62-63 22.77126087742101 26.928396990167336 26.83581724184157 23.464524890570086 64-65 22.706443567984223 26.83693526776295 26.642797541652648 23.81382362260018 66-67 22.70314542566518 27.072697561609083 26.42485826548239 23.799298747243355 68-69 22.690879858770106 26.692743153290248 26.96911318884718 23.64726379909246 70-71 22.601969398688997 26.640621575724115 26.81188782690032 23.94552119868657 72-73 22.715284840125545 26.701130146615505 26.561639549582694 24.021945463676257 74-75 22.61570090128569 26.89546736622116 26.691280390822918 23.797551341670232 76-77 22.867571579480156 26.64690098718821 26.640746069303912 23.844781364027725 78-79 22.80650667246107 26.66841835429802 26.855492997873494 23.669581975367418 80-81 22.80749977793305 26.98730103442399 26.66213906543503 23.543060122207933 82-83 22.760214236272123 26.726961126136935 26.8051326993346 23.707691938256346 84-85 22.756949165503304 26.628403941722784 26.751081530471694 23.863565362302218 86-87 22.80577044086375 26.616050073218116 26.80440516371319 23.77377432220494 88-89 22.773806084862034 26.651353441500476 26.81532712084555 23.75951335279195 90-91 22.805923020503588 26.82830617038033 26.76046290092225 23.605307908193833 92-93 22.827144447488212 26.191489237691684 26.968100669693595 24.01326564512651 94-95 22.560873397833806 26.173118928601152 27.15538374702834 24.1106239265367 96-97 22.772371175456712 26.121120377832945 27.135387730827134 23.97112071588321 98-99 22.67936684974315 25.98577104513063 27.016782762243192 24.318079342883028 100-101 22.4389583145363 26.068341694952558 27.274312634799493 24.218387355711652 102-103 22.50877894042817 25.801738395735192 27.35052020846574 24.3389624553709 104-105 22.466483185347606 26.08134493905133 27.195778297680373 24.256393577920694 106-107 22.529941655506356 26.017134964541917 27.079734987670808 24.37318839228092 108-109 22.549145322061037 25.895955859552956 27.04875340912959 24.50614540925642 110-111 22.335120057763323 26.23922678841665 27.212618963743353 24.213034190076673 112-113 22.268734663425953 26.208233518100798 27.512703491930267 24.010328326542986 114-115 22.25166594313761 26.27949235771463 27.20825186831367 24.26058983083409 116-117 22.056784217177018 26.154429010402826 27.430162127379802 24.358624645040358 118-119 21.94685047450935 26.323606793525922 27.124632875679673 24.604909856285058 120-121 22.024719015716187 26.581557432760917 27.148923827220113 24.244799724302787 122-123 22.06723474339091 26.607403151995808 27.132525951879792 24.192836152733495 124-125 22.086052486108628 26.479486838138726 27.180297926896397 24.25416274885625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 410.0 1 365.5 2 285.0 3 224.0 4 177.5 5 149.0 6 138.5 7 129.5 8 126.5 9 138.0 10 140.0 11 134.5 12 131.0 13 138.5 14 153.0 15 168.5 16 218.0 17 255.5 18 366.5 19 487.5 20 603.5 21 904.5 22 1287.0 23 1860.0 24 2819.5 25 4205.5 26 6219.5 27 8687.0 28 11357.0 29 14929.5 30 19559.0 31 24821.5 32 31534.0 33 36181.0 34 39610.5 35 44185.0 36 49469.5 37 55225.5 38 59596.5 39 62454.0 40 63796.5 41 66732.5 42 69940.5 43 74125.0 44 79753.5 45 83580.0 46 83825.0 47 82955.0 48 83422.0 49 82882.0 50 81650.0 51 79699.0 52 79014.0 53 78694.0 54 78008.0 55 73705.5 56 65994.0 57 60348.5 58 56710.5 59 51454.5 60 42708.0 61 34457.0 62 27522.5 63 20765.5 64 16027.5 65 12698.5 66 9329.0 67 7806.0 68 5938.5 69 3425.5 70 2264.0 71 1595.0 72 1229.0 73 916.5 74 630.5 75 468.5 76 307.5 77 188.5 78 111.5 79 65.5 80 47.5 81 34.5 82 30.5 83 25.0 84 19.0 85 16.5 86 14.5 87 12.5 88 9.0 89 10.0 90 9.0 91 8.5 92 7.0 93 5.0 94 5.0 95 3.5 96 1.5 97 0.5 98 2.0 99 2.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04308385264762864 2 0.007600290023337087 3 0.001678591661595921 4 0.005362167807875859 5 0.021495298777658878 6 0.026950721677845624 7 0.0027043976770156504 8 4.6627546155442255E-5 9 0.0 10-11 1.1656886538860564E-4 12-13 9.791784692642872E-4 14-15 3.2639282308809573E-4 16-17 9.325509231088451E-5 18-19 2.3313773077721127E-5 20-21 6.994131923316338E-5 22-23 0.0 24-25 0.0 26-27 6.994131923316338E-5 28-29 1.8651018462176902E-4 30-31 1.8651018462176902E-4 32-33 1.1656886538860564E-4 34-35 8.159820577202393E-4 36-37 1.6319641154404786E-4 38-39 1.3988263846632676E-4 40-41 4.6627546155442255E-4 42-43 5.595305538653071E-4 44-45 0.0012589437461969408 46-47 4.6627546155442255E-5 48-49 0.0 50-51 0.0 52-53 7.926682846425183E-4 54-55 0.002611142584704766 56-57 6.994131923316338E-5 58-59 8.859233769534027E-4 60-61 0.0011656886538860562 62-63 8.159820577202393E-4 64-65 0.002028298257761738 66-67 0.003007476727026025 68-69 0.0031007318193369098 70-71 0.001911729392373132 72-73 0.0030774180462591885 74-75 0.002424632400082997 76-77 0.0013288850654301042 78-79 0.002634456357782487 80-81 0.0014687677038964309 82-83 6.294718730984704E-4 84-85 4.1964791539898024E-4 86-87 0.0016552778885182 88-89 0.004825951027088273 90-91 2.7976527693265353E-4 92-93 8.159820577202393E-4 94-95 9.092371500311238E-4 96-97 1.6319641154404786E-4 98-99 6.527856461761915E-4 100-101 0.0016086503423627578 102-103 0.0015853365692850366 104-105 0.0010491197884974505 106-107 0.007040759469471779 108-109 0.0021448671231503436 110-111 0.0019350431654508533 112-113 0.0031473593654923523 114-115 0.003450438415502727 116-117 0.002098239576994901 118-119 0.04508883713231266 120-121 0.012752633873513455 122-123 0.005082402530943206 124-125 0.0031939869116477944 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2144655.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.65497758026653 #Duplication Level Percentage of deduplicated Percentage of total 1 82.85327186512346 53.56876434891284 2 9.70561750173754 12.550329639549663 3 3.1127222811368878 6.0375896790150465 4 1.4515884607711504 3.754096775477293 5 0.7707287654110294 2.49157255240583 6 0.46535796169824084 1.8052625148238977 7 0.28903704764805155 1.3081378684885843 8 0.21888744942526683 1.1321730508153878 9 0.1637762022939597 0.9530052018747444 >10 0.8656239644422035 10.176203341159903 >50 0.07902396333703525 3.4785998336085653 >100 0.02419050457689311 2.6757401435720944 >500 1.7403239820169679E-4 0.0685250502961817 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 9.32550923108845E-5 0.0 18-19 0.0 0.0 0.0 9.32550923108845E-5 0.0 20-21 0.0 0.0 0.0 1.6319641154404786E-4 0.0 22-23 0.0 0.0 0.0 3.2639282308809573E-4 0.0 24-25 4.662754615544225E-5 0.0 0.0 6.994131923316338E-4 0.0 26-27 4.662754615544225E-5 0.0 0.0 0.0012356299731192196 0.0 28-29 4.662754615544225E-5 0.0 0.0 0.003543693507813611 0.0 30-31 4.662754615544225E-5 0.0 0.0 0.0132422231081456 0.0 32-33 4.662754615544225E-5 0.0 0.0 0.033292067954985766 0.0 34-35 4.662754615544225E-5 0.0 0.0 0.06283061844445843 0.0 36-37 4.662754615544225E-5 0.0 0.0 0.11652223784245018 0.0 38-39 9.32550923108845E-5 0.0 0.0 0.20035856582993536 0.0 40-41 9.32550923108845E-5 0.0 0.0 0.2928909311754105 0.0 42-43 1.1656886538860564E-4 0.0 0.0 0.3831618605323467 0.0 44-45 1.3988263846632676E-4 0.0 0.0 0.49350594850920076 0.0 46-47 1.3988263846632676E-4 0.0 0.0 0.6319198192716311 0.0 48-49 1.3988263846632676E-4 0.0 0.0 0.7819439490267666 0.0 50-51 1.3988263846632676E-4 0.0 0.0 0.9362811268012804 0.0 52-53 1.3988263846632676E-4 0.0 0.0 1.088450123679566 0.0 54-55 1.3988263846632676E-4 0.0 0.0 1.2559362694699148 0.0 56-57 1.3988263846632676E-4 0.0 0.0 1.4483681524534249 0.0 58-59 1.3988263846632676E-4 0.0 0.0 1.656373635852853 0.0 60-61 1.3988263846632676E-4 0.0 0.0 1.8890450911685095 0.0 62-63 1.3988263846632676E-4 0.0 0.0 2.1177532050609535 0.0 64-65 1.3988263846632676E-4 0.0 0.0 2.355320552722932 0.0 66-67 1.3988263846632676E-4 0.0 0.0 2.624151670082134 0.0 68-69 1.3988263846632676E-4 0.0 0.0 2.9290025668464157 0.0 70-71 1.3988263846632676E-4 0.0 0.0 3.2614802847077966 0.0 72-73 1.3988263846632676E-4 0.0 0.0 3.6008355656271056 0.0 74-75 1.3988263846632676E-4 0.0 0.0 3.929326628292196 0.0 76-77 1.3988263846632676E-4 0.0 0.0 4.295049786562408 0.0 78-79 1.3988263846632676E-4 0.0 0.0 4.698564570991605 0.0 80-81 1.3988263846632676E-4 0.0 0.0 5.133879341898814 0.0 82-83 1.3988263846632676E-4 0.0 0.0 5.591085745726002 0.0 84-85 1.3988263846632676E-4 0.0 0.0 6.041624410452963 0.0 86-87 1.3988263846632676E-4 0.0 0.0 6.50141864309178 0.0 88-89 1.3988263846632676E-4 0.0 0.0 6.990984563950845 0.0 90-91 1.3988263846632676E-4 0.0 0.0 7.537506032438784 0.0 92-93 1.86510184621769E-4 0.0 0.0 8.095987466515593 0.0 94-95 1.86510184621769E-4 0.0 0.0 8.668876812354435 0.0 96-97 1.86510184621769E-4 0.0 0.0 9.241463079143266 0.0 98-99 1.86510184621769E-4 0.0 0.0 9.840533792148388 0.0 100-101 1.86510184621769E-4 0.0 0.0 10.501246121171004 0.0 102-103 1.86510184621769E-4 0.0 0.0 11.194271339679343 0.0 104-105 1.86510184621769E-4 0.0 0.0 11.887995971380011 0.0 106-107 1.86510184621769E-4 0.0 0.0 12.570296854272598 0.0 108-109 1.86510184621769E-4 0.0 0.0 13.278662535466077 0.0 110-111 1.86510184621769E-4 0.0 0.0 14.021019697806873 0.0 112-113 1.86510184621769E-4 0.0 0.0 14.79345162741793 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3085 0.0 49.41719 1 GGTATCA 2145 0.0 48.30757 1 TCAACGC 3530 0.0 43.000782 4 ATCAACG 3560 0.0 42.303993 3 CAACGCA 3725 0.0 40.749733 5 AACGCAG 3810 0.0 39.833183 6 TATCAAC 3980 0.0 37.839752 2 GTACATG 9200 0.0 35.925137 1 TACATGG 9465 0.0 34.27573 2 ACATGGG 9565 0.0 33.10835 3 ACGCAGA 4835 0.0 30.885536 7 CGCAGAG 4880 0.0 30.478817 8 CATGGGG 5130 0.0 30.285448 4 GAGTACA 7365 0.0 24.742405 1 ATGGGGA 3120 0.0 24.611946 5 CATGGGA 5330 0.0 24.123337 4 GCAGAGT 5955 0.0 23.977694 9 AGTACAT 7470 0.0 23.348438 2 CCTACGC 535 0.0 22.796482 96-97 TCGCGGA 525 0.0 22.665686 82-83 >>END_MODULE