FastQCFastQC Report
Thu 26 May 2016
SRR1364361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144655
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23330.10878206518064677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.10104189251884335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30150.060.6445731
GTATCAA48350.053.953371
TCAACGC61450.041.8621644
ATCAACG61800.041.6250843
CAACGCA63750.040.351855
AACGCAG65600.039.2762376
TATCAAC66600.038.9827162
GTACATG85250.034.582131
TACATGG87000.033.1272932
ACATGGG86700.032.761153
ACGCAGA79350.032.316597
CGCAGAG80850.031.7906138
ACCGTAT1306.382982E-527.460168
GCAGAGT95550.026.837489
CATGGGG49550.026.1982734
GAGTACT47100.024.5693612-13
CATGGGA49200.024.5691874
AGAGTAC89200.024.54585510-11
GTGGTAT19100.024.322161
CAGAGTA90300.023.81857310-11
Produced by FastQC (version 0.11.5)