##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364361_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2144655 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.278574875679304 33.0 31.0 34.0 30.0 34.0 2 32.290955421734495 33.0 31.0 34.0 30.0 34.0 3 32.41388055421501 34.0 31.0 34.0 30.0 34.0 4 36.02484595424439 37.0 35.0 37.0 35.0 37.0 5 35.95643541735151 37.0 35.0 37.0 35.0 37.0 6 36.00869370598068 37.0 35.0 37.0 35.0 37.0 7 35.95394690521319 37.0 35.0 37.0 35.0 37.0 8 35.955059904739926 37.0 35.0 37.0 35.0 37.0 9 37.7032981994773 39.0 37.0 39.0 35.0 39.0 10-11 37.661292841972255 39.0 37.0 39.0 35.0 39.0 12-13 37.645716910179026 39.0 37.0 39.0 35.0 39.0 14-15 39.05264483098681 41.0 38.0 41.0 35.0 41.0 16-17 39.0530341709972 41.0 38.0 41.0 35.0 41.0 18-19 39.09154875725933 41.0 38.5 41.0 35.0 41.0 20-21 39.09483203592186 41.0 39.0 41.0 35.0 41.0 22-23 39.06410541555635 41.0 39.0 41.0 35.0 41.0 24-25 39.03797627124176 41.0 39.0 41.0 35.0 41.0 26-27 38.953351704586524 41.0 38.0 41.0 35.0 41.0 28-29 38.86503540196442 40.5 38.0 41.0 35.0 41.0 30-31 38.773411574355784 40.0 38.0 41.0 35.0 41.0 32-33 38.674696862665556 40.0 38.0 41.0 34.0 41.0 34-35 38.5984797088576 40.0 38.0 41.0 34.0 41.0 36-37 38.5000636466005 40.0 38.0 41.0 34.0 41.0 38-39 38.420965842991066 40.0 38.0 41.0 34.0 41.0 40-41 38.32815604374596 40.0 38.0 41.0 34.0 41.0 42-43 38.20132422231082 40.0 38.0 41.0 33.0 41.0 44-45 38.070983444889734 40.0 38.0 41.0 33.0 41.0 46-47 37.90571630402093 40.0 37.5 41.0 33.0 41.0 48-49 37.74316871478163 40.0 37.0 41.0 33.0 41.0 50-51 37.59515329971487 40.0 37.0 41.0 32.5 41.0 52-53 37.44328015461694 40.0 37.0 41.0 32.0 41.0 54-55 37.264118238131545 40.0 36.0 41.0 32.0 41.0 56-57 37.07064679400649 39.5 36.0 41.0 31.0 41.0 58-59 36.85299267248112 39.0 35.5 41.0 31.0 41.0 60-61 36.59775371796396 39.0 35.0 41.0 31.0 41.0 62-63 36.35391636417046 38.5 35.0 41.0 31.0 41.0 64-65 36.05945501724054 38.0 35.0 40.0 30.5 41.0 66-67 35.73460230200195 37.0 35.0 40.0 30.0 41.0 68-69 35.39724198064491 37.0 35.0 39.5 30.0 41.0 70-71 35.132087911575525 36.0 34.5 39.0 29.5 41.0 72-73 34.92949425432062 36.0 34.5 39.0 30.0 41.0 74-75 34.5868202578037 35.5 34.0 38.5 30.0 40.0 76-77 34.25324143043986 35.0 34.0 37.0 29.0 39.0 78-79 33.92959893316174 35.0 34.0 37.0 29.0 39.0 80-81 33.63261550226027 35.0 34.0 36.0 29.0 39.0 82-83 33.36276510674211 35.0 34.0 36.0 29.0 37.0 84-85 33.12878854640956 35.0 34.0 35.5 29.0 37.0 86-87 32.9507722687332 35.0 34.0 35.0 29.0 36.5 88-89 32.79634533293233 35.0 34.0 35.0 29.0 36.0 90-91 32.65618059781177 35.0 34.0 35.0 29.0 36.0 92-93 32.53536209786655 35.0 33.0 35.0 29.0 36.0 94-95 32.455975203470956 35.0 33.0 35.0 29.0 35.5 96-97 32.37652699385216 35.0 33.0 35.0 27.5 35.0 98-99 32.304473446778154 35.0 33.0 35.0 27.0 35.0 100-101 32.24361377470969 35.0 33.0 35.0 27.0 35.0 102-103 31.248605719801084 34.0 31.5 35.0 25.0 35.0 104-105 31.979451473547027 35.0 33.0 35.0 26.5 35.0 106-107 32.27997113754893 35.0 33.0 35.0 28.0 35.0 108-109 32.29632131974607 35.0 34.0 35.0 29.0 35.0 110-111 32.26533987984081 35.0 34.0 35.0 29.0 35.0 112-113 32.198302291044484 35.0 34.0 35.0 28.0 35.0 114-115 32.11830037931509 35.0 33.0 35.0 27.0 35.0 116-117 32.05914168013037 35.0 33.0 35.0 27.0 35.0 118-119 31.948010752312143 35.0 33.0 35.0 27.0 35.0 120-121 31.80664395905169 35.0 33.0 35.0 27.0 35.0 122-123 31.6460325786665 35.0 33.0 35.0 25.0 35.0 124-125 30.932608041852887 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11842361842198557 1101 2 0.18646548440572985 1101 3 0.18037683217136902 1101 4 0.17862659833751593 1101 5 0.27920169119661864 1101 6 0.28068055158644256 1101 7 0.2580147040695664 1101 8 0.27148162973873013 1101 9 0.2354433449598261 1101 10-11 0.28071894192695623 1101 12-13 0.29201149257582415 1101 14-15 0.39174160262636093 1101 16-17 0.33453707084092343 1101 18-19 0.3585726552070909 1101 20-21 0.32372362837790547 1101 22-23 0.35511578643939856 1101 24-25 0.41987667991777045 1101 26-27 0.36474254862393707 1101 28-29 0.28790255725239433 1101 30-31 0.32448788261832107 1101 32-33 0.2400733492591982 1101 34-35 0.31124412604404483 1101 36-37 0.3366342307982677 1101 38-39 0.3175400221672433 1101 40-41 0.304544474636927 1101 42-43 0.32722068083615596 1101 44-45 0.23313548735143286 1101 46-47 0.3014159374444674 1101 48-49 0.20177337072962587 1101 50-51 0.27420575556013915 1101 52-53 0.25135822970861454 1101 54-55 0.30035627716857505 1101 56-57 0.32081708465990033 1101 58-59 0.2278367335580569 1101 60-61 0.29488494943765176 1101 62-63 0.17111511320140238 1101 64-65 0.11086610037144595 1101 66-67 0.13195585914752428 1101 68-69 0.241136394997568 1101 70-71 0.25635383612890905 1101 72-73 0.16842660490921446 1101 74-75 0.20728137999173413 1101 76-77 0.013703877952913501 1101 78-79 -0.08513456546134535 1101 80-81 0.004216929455168383 1101 82-83 -0.02075788179805471 1101 84-85 -0.16953142919720676 1101 86-87 -0.046161332468251715 1101 88-89 -0.17784508911667274 1101 90-91 -0.6106707696935914 1101 92-93 -0.427886697712907 1101 94-95 -0.2802486843741292 1101 96-97 -0.17935787495912336 1101 98-99 -0.09579621508697755 1101 100-101 -0.11951639615557497 1101 102-103 -0.031050326725981847 1101 104-105 0.002797659375978867 1101 106-107 0.054117263634609 1101 108-109 0.14263176299779445 1101 110-111 0.1169554645127846 1101 112-113 0.1198501485846748 1101 114-115 -0.06187735202146882 1101 116-117 0.09780648953072557 1101 118-119 0.025788937628611563 1101 120-121 0.09396846677553583 1101 122-123 0.1561657556348095 1101 124-125 0.13532787012634628 1102 1 0.15214355257167966 1102 2 0.14996464272169874 1102 3 0.11254905732004516 1102 4 0.04007922229906313 1102 5 0.004183883368597208 1102 6 -0.04564245921989851 1102 7 0.11488522698574144 1102 8 0.1936145694271474 1102 9 0.2490108997672209 1102 10-11 0.2173719361676376 1102 12-13 0.23016415849272676 1102 14-15 0.3210662246416831 1102 16-17 0.330310610934049 1102 18-19 0.36082144113070314 1102 20-21 0.31246134973874007 1102 22-23 0.35086072061486107 1102 24-25 0.3781845599976421 1102 26-27 0.3461081899789207 1102 28-29 0.3720366844729668 1102 30-31 0.33308769768493107 1102 32-33 0.3357121580572482 1102 34-35 0.38104076116345453 1102 36-37 0.42083866157142324 1102 38-39 0.3809374361922835 1102 40-41 0.3486282554424065 1102 42-43 0.2942138844824598 1102 44-45 0.35000565978068465 1102 46-47 0.425749161851968 1102 48-49 0.4758306382305193 1102 50-51 0.3598892926741186 1102 52-53 0.4372423690415985 1102 54-55 0.36041246846846064 1102 56-57 0.37958781364176986 1102 58-59 0.3969887304572808 1102 60-61 0.36864894185476516 1102 62-63 0.3374122395179242 1102 64-65 0.2847699267608448 1102 66-67 0.22473701408439695 1102 68-69 0.17461423546211563 1102 70-71 0.16552391402977662 1102 72-73 0.24105166946581136 1102 74-75 0.2292345920497212 1102 76-77 0.33881281237989924 1102 78-79 0.30662723950505466 1102 80-81 0.25853662919097076 1102 82-83 0.22354562338039585 1102 84-85 0.2978415373343779 1102 86-87 0.2870733834672592 1102 88-89 0.2208700155294423 1102 90-91 0.25227374307289807 1102 92-93 0.2341847933718384 1102 94-95 0.22040074727009795 1102 96-97 0.25273064566651726 1102 98-99 0.22927777373249114 1102 100-101 0.24300577987339267 1102 102-103 0.24637336763292694 1102 104-105 0.1490603348900308 1102 106-107 0.13998300240434247 1102 108-109 0.1632299307758842 1102 110-111 0.17902994504478187 1102 112-113 0.1001420114123377 1102 114-115 0.02120701533418412 1102 116-117 -4.901955846179362E-4 1102 118-119 -0.06550939992946425 1102 120-121 0.034150348869676606 1102 122-123 -0.03632413436384141 1102 124-125 -0.10810472660030968 1103 1 0.14912564704062703 1103 2 0.1399917401129116 1103 3 0.1898191279857926 1103 4 0.09961378475589555 1103 5 0.03275834806229483 1103 6 0.06933017772819028 1103 7 0.05443679172725524 1103 8 0.14263959466491372 1103 9 0.23677988122410198 1103 10-11 0.2604302221248176 1103 12-13 0.24801655740882467 1103 14-15 0.4218897728059616 1103 16-17 0.3894094898459102 1103 18-19 0.33036078548010295 1103 20-21 0.29064529320613985 1103 22-23 0.3393868350614966 1103 24-25 0.30145378054914573 1103 26-27 0.27687169882543117 1103 28-29 0.34723725830007623 1103 30-31 0.34401697880708326 1103 32-33 0.3056712463097284 1103 34-35 0.2758470945144964 1103 36-37 0.31597441418818306 1103 38-39 0.32107876768370147 1103 40-41 0.2606068963222796 1103 42-43 0.22452577014210107 1103 44-45 0.26700263124872947 1103 46-47 0.3031069005481086 1103 48-49 0.23033358706426554 1103 50-51 0.11412658080971738 1103 52-53 0.25978102267318093 1103 54-55 0.23394469311114108 1103 56-57 0.20936040808414447 1103 58-59 0.15766032070222025 1103 60-61 0.2970693233435284 1103 62-63 0.2513780224200062 1103 64-65 0.17352450940788344 1103 66-67 0.1317371734808077 1103 68-69 0.16895831919836724 1103 70-71 0.17301554571778865 1103 72-73 0.2243070756610166 1103 74-75 0.23832815774760974 1103 76-77 0.2067130302216711 1103 78-79 0.2590633481071478 1103 80-81 0.19746123468482324 1103 82-83 0.20447649846672533 1103 84-85 0.24645213188302506 1103 86-87 0.21397418576256655 1103 88-89 0.22544203614471314 1103 90-91 0.23840891123611385 1103 92-93 0.13946347144420201 1103 94-95 0.24448554616494533 1103 96-97 0.18470025408264945 1103 98-99 0.20223617126715965 1103 100-101 0.1389916467242429 1103 102-103 0.2966044924403235 1103 104-105 0.2804481463772035 1103 106-107 0.23741937666713397 1103 108-109 0.17909253106279266 1103 110-111 0.19971694361021264 1103 112-113 0.23372949348024719 1103 114-115 0.10689853013147399 1103 116-117 0.0935882274534805 1103 118-119 0.06214056553464431 1103 120-121 0.07580063312662233 1103 122-123 0.08425394331534974 1103 124-125 -0.0451670506000994 1104 1 0.07091754106194514 1104 2 0.04599585987008936 1104 3 0.07981816350972792 1104 4 0.08056738600235036 1104 5 0.12050311880819464 1104 6 0.11566323507538101 1104 7 0.17148057474716438 1104 8 0.13044600801139694 1104 9 0.15978039887285433 1104 10-11 0.13595084117866207 1104 12-13 0.17246248454755175 1104 14-15 0.2214877680003582 1104 16-17 0.20067252017378223 1104 18-19 0.19150926680304536 1104 20-21 0.14539538441203348 1104 22-23 0.18502388547936732 1104 24-25 0.17092452547412051 1104 26-27 0.09770577958887827 1104 28-29 0.20735584692214104 1104 30-31 0.22956880122421097 1104 32-33 0.18237360480315346 1104 34-35 0.18550867523951098 1104 36-37 0.24754078388630774 1104 38-39 0.3309897143938372 1104 40-41 0.1151695902949541 1104 42-43 0.20492044120216946 1104 44-45 0.17077238145874674 1104 46-47 0.11417880619904253 1104 48-49 0.13868278144718715 1104 50-51 0.11803592378718264 1104 52-53 0.1619153103563562 1104 54-55 0.16135598810430452 1104 56-57 0.19089502462104235 1104 58-59 0.2271664763425676 1104 60-61 0.23744364256088346 1104 62-63 0.20518208840879737 1104 64-65 0.2068965926249362 1104 66-67 0.13330954902262704 1104 68-69 0.16410290441075404 1104 70-71 0.2704518099245945 1104 72-73 0.3562847221810017 1104 74-75 0.3726356895601839 1104 76-77 0.3968568303412283 1104 78-79 0.40886851928301127 1104 80-81 0.363706436183449 1104 82-83 0.3278869417390027 1104 84-85 0.3790012887119545 1104 86-87 0.32421934439557987 1104 88-89 0.36464402771789395 1104 90-91 0.3363653868076213 1104 92-93 0.3259000492222057 1104 94-95 0.27981826478068683 1104 96-97 0.2241534753628116 1104 98-99 0.20709754996472896 1104 100-101 0.21562295082168959 1104 102-103 0.23788723628619834 1104 104-105 0.27311044044176214 1104 106-107 0.25996090621434575 1104 108-109 0.2356739153701568 1104 110-111 0.23694637196478396 1104 112-113 0.04639371649692947 1104 114-115 0.10362760507779001 1104 116-117 0.06695233394096078 1104 118-119 -8.766696992061895E-4 1104 120-121 0.03168535820202223 1104 122-123 -0.16944794412237485 1104 124-125 -0.328465213948796 1105 1 0.10219007352042553 1105 2 0.14628734065044569 1105 3 0.1260840291450478 1105 4 0.1239204689650677 1105 5 0.11346183053034764 1105 6 0.15149709885494644 1105 7 0.16840817493476123 1105 8 0.1946699985411442 1105 9 0.18964730634599647 1105 10-11 0.20100992207297708 1105 12-13 0.20462630475458354 1105 14-15 0.2469490491074069 1105 16-17 0.21843130774629316 1105 18-19 0.2639333347480388 1105 20-21 0.22650399632743756 1105 22-23 0.16117143283865687 1105 24-25 0.2160194511863338 1105 26-27 0.20903123240125865 1105 28-29 0.12137918839742667 1105 30-31 0.20282188414089575 1105 32-33 0.14448004476561493 1105 34-35 0.1710306458392168 1105 36-37 0.049428528115946335 1105 38-39 0.16106511830390957 1105 40-41 0.15486993534965166 1105 42-43 0.20388260761362886 1105 44-45 0.14294735829631122 1105 46-47 0.15397640356527376 1105 48-49 0.2243259837499494 1105 50-51 0.10405699711579786 1105 52-53 0.09408810557557956 1105 54-55 0.11029843250180704 1105 56-57 0.0791762821721349 1105 58-59 -0.004154014898972491 1105 60-61 0.03824237261967056 1105 62-63 0.05832161670977598 1105 64-65 0.012614503849633252 1105 66-67 0.12244269632006421 1105 68-69 0.03062044779804296 1105 70-71 -0.056036995013414526 1105 72-73 0.05323133098416122 1105 74-75 0.17365589530427883 1105 76-77 0.08743292946954995 1105 78-79 0.15470938357208297 1105 80-81 0.1588935283669457 1105 82-83 0.1443092349328552 1105 84-85 0.22070264725265076 1105 86-87 0.22857245543396942 1105 88-89 0.2714411496386049 1105 90-91 0.2482517088430214 1105 92-93 0.2770921379560818 1105 94-95 0.1457071891093662 1105 96-97 0.14560697628493813 1105 98-99 0.14229147236262918 1105 100-101 0.22274865023978663 1105 102-103 0.31749554834414795 1105 104-105 0.24717063009527607 1105 106-107 0.2110620021323797 1105 108-109 0.23938901810643642 1105 110-111 0.19887101039837063 1105 112-113 0.16749599527553727 1105 114-115 0.17506920835555917 1105 116-117 0.21768899833855926 1105 118-119 0.07596724655426712 1105 120-121 0.18151296892950697 1105 122-123 0.2652738005831736 1105 124-125 0.1315222946752712 1106 1 0.10953251697926447 1106 2 0.07961482165475786 1106 3 0.08652470023069014 1106 4 0.08096195872650469 1106 5 0.035531845751044955 1106 6 0.024635233792004385 1106 7 0.1357662989196058 1106 8 0.018360040617537265 1106 9 0.1524758205650656 1106 10-11 0.0801566339233446 1106 12-13 0.10403438347677252 1106 14-15 0.10060682441419999 1106 16-17 0.11445353544926462 1106 18-19 0.1506929977096192 1106 20-21 0.12095858945355786 1106 22-23 -0.007775226898104393 1106 24-25 0.17117565495700404 1106 26-27 0.13116381180598324 1106 28-29 0.064736493056202 1106 30-31 0.05034572193105902 1106 32-33 0.14481971208393674 1106 34-35 0.05773368983990679 1106 36-37 5.998067430112997E-4 1106 38-39 0.019548138885994604 1106 40-41 0.040260013979875 1106 42-43 0.02225227880099112 1106 44-45 0.011824951261253602 1106 46-47 -0.11438316538747273 1106 48-49 0.007175826457000767 1106 50-51 -0.05760453482886163 1106 52-53 -0.1972043255515672 1106 54-55 -0.2799528878718718 1106 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-0.8178523880105502 2216 46-47 -0.9913098650714218 2216 48-49 -1.0244940011581463 2216 50-51 -0.8595552254572922 2216 52-53 -0.9121027265619119 2216 54-55 -0.8251696184624535 2216 56-57 -0.9778381865978929 2216 58-59 -0.9428133941058192 2216 60-61 -1.0190735788251004 2216 62-63 -0.9306589872238007 2216 64-65 -0.8667167002734075 2216 66-67 -0.860208325936263 2216 68-69 -1.0631328915844875 2216 70-71 -1.0805855815579974 2216 72-73 -1.0901082691737756 2216 74-75 -1.1353777479059914 2216 76-77 -1.10776022507941 2216 78-79 -0.9864874600091298 2216 80-81 -0.9368579553959364 2216 82-83 -0.9072334654156649 2216 84-85 -0.8775400214286435 2216 86-87 -0.903345135156286 2216 88-89 -0.8500869251582372 2216 90-91 -0.9359585135870603 2216 92-93 -0.7577129886706402 2216 94-95 -0.8207655213344651 2216 96-97 -0.8908154885269113 2216 98-99 -0.9337948845692274 2216 100-101 -0.9731049414590487 2216 102-103 -1.0527671475274296 2216 104-105 -1.0405952491544284 2216 106-107 -0.9381589973706355 2216 108-109 -0.7682851993954607 2216 110-111 -0.6566352455447912 2216 112-113 -0.5431163014690448 2216 114-115 -0.5859800561697277 2216 116-117 -0.45633059448217494 2216 118-119 -0.5336096695018178 2216 120-121 -0.4981982419355937 2216 122-123 -0.6001760095244926 2216 124-125 -0.6086609311094051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 290.0 3 15.0 4 43.0 5 129.0 6 271.0 7 606.0 8 1104.0 9 1912.0 10 3047.0 11 3848.0 12 5112.0 13 6848.0 14 9725.0 15 8140.0 16 4603.0 17 4359.0 18 4393.0 19 4439.0 20 4626.0 21 4901.0 22 5251.0 23 5945.0 24 7584.0 25 10410.0 26 14163.0 27 18389.0 28 24607.0 29 31154.0 30 39622.0 31 49837.0 32 62925.0 33 81772.0 34 111298.0 35 168595.0 36 337060.0 37 740056.0 38 366248.0 39 1328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.022379392787215 18.978314628647226 12.980999297583146 25.018306680982416 2 15.761835819747233 20.75028384518722 38.34994439665121 25.137935938414337 3 18.268439445971495 24.975905215524175 30.185041416917873 26.570613921586457 4 12.65756030690251 16.737890243419105 37.164812056018334 33.43973739366005 5 13.058950864948587 38.41435422770448 34.97135499281542 13.555339914531508 6 32.293840502493296 37.225271842663524 17.0151099612143 13.465777693628878 7 28.12419713193964 32.61326413805484 21.63196411544048 17.63057461456505 8 26.539466720754618 35.13637391561813 20.025738405477806 18.298420958149446 9 26.697067826760012 14.980031753358933 19.663675509580795 38.65922491030026 10-11 24.82597434086135 26.317099953139316 28.1071780775929 20.749747628406435 12-13 25.496152061753524 23.68283010554145 28.243190629728325 22.577827202976703 14-15 22.41376818182878 25.115578030032804 25.735654452580953 26.734999335557468 16-17 22.613357393147147 28.51936558560701 26.35078835523662 22.51648866600922 18-19 22.546283668002545 27.105408562216297 28.056284110964235 22.29202365881692 20-21 23.03759263170803 26.494825027312164 28.185591208631344 22.281991132348463 22-23 23.027246806595933 26.31185435419683 27.87686131335809 22.784037525849147 24-25 22.793764683309128 26.593164540022645 27.9671387629103 22.64593201375793 26-27 22.538844943588657 26.945744255993464 27.817402245636057 22.698008554781822 28-29 22.545017856068757 26.866494237518584 27.950307952490128 22.63817995392253 30-31 22.63044724536702 26.47932611863798 27.970618056415255 22.91960857957975 32-33 22.536492542724325 26.56908433332144 27.985632157780486 22.908790966173743 34-35 23.097824819900683 26.48164036089805 27.593500104912223 22.827034714289045 36-37 23.122273745660724 26.22808796752858 27.56685807274363 23.082780214067064 38-39 22.700434335592437 26.53608622365835 27.703453469206003 23.060025971543208 40-41 22.88688861570283 26.16378465219174 27.87072864752152 23.078598084583906 42-43 22.9901312798562 26.172368049872823 27.706880593848425 23.13062007642255 44-45 22.750536742466895 26.29649912654766 27.696470823567754 23.25649330741769 46-47 23.11567594787973 26.626263897922975 27.146813823202333 23.111246330994963 48-49 23.1000612456674 26.701175930802425 26.956463352965987 23.242299470564195 50-51 22.90132911820316 26.646406997862126 27.192765269938523 23.25949861399619 52-53 23.28479405778552 26.51114048646519 27.003061098405105 23.20100435734419 54-55 22.787627846903117 26.46344050674817 27.20759282961595 23.54133881673276 56-57 22.877586405063948 26.687599360548788 27.196353346919068 23.238460887468197 58-59 22.982326523922616 26.3263849724979 27.106720453107947 23.584568050471532 60-61 22.77221744289874 26.424016916473747 27.555667461666328 23.248098178961186 62-63 22.735661446712875 26.579379900263678 27.39291401181076 23.29204464121269 64-65 22.535466077294483 26.454604586751717 27.293457455861198 23.7164718800926 66-67 22.599135991569742 26.676924726820868 27.075427050038353 23.648512231571043 68-69 22.56385472058773 26.40762249006658 27.43382555736859 23.5946972319771 70-71 22.462872224873518 26.370657837894168 27.287378922805516 23.8790910144268 72-73 22.642723071790453 26.472249687186938 26.928151263736787 23.956875977285822 74-75 22.604358276739152 26.661281185085713 26.97897797081582 23.75538256735932 76-77 22.821280262457254 26.462660177352614 26.909608729426754 23.806450830763374 78-79 22.735428308982097 26.561288412355367 26.994248492181725 23.70903478648081 80-81 22.765900551162066 26.876383821046833 26.73207126289342 23.62564436489768 82-83 22.762775364802266 26.61588926890339 26.856813799888563 23.764521566405787 84-85 22.7785174721616 26.50783611066491 26.737826535696072 23.975819881477413 86-87 22.819171381877272 26.53312537447748 26.76931720952787 23.878386034117373 88-89 22.657070717667878 26.632511989107805 26.73987191413071 23.970545379093608 90-91 22.74632114403509 26.859920793768882 26.623192686747444 23.770565375448584 92-93 22.71957494328925 26.249699835171626 26.799788310940453 24.230936910598675 94-95 22.50571770284731 26.202839151285406 26.930485322814157 24.360957823053127 96-97 22.682575985414903 26.148191667191227 26.9531463102457 24.216086037148166 98-99 22.623523040234296 26.04505955632398 26.714725779328614 24.616691624113113 100-101 22.376466144904423 26.05680167672656 26.98786051835843 24.578871660010584 102-103 22.42288386710217 25.796270262583025 27.00588206494751 24.774963805367296 104-105 22.461453096167048 26.03722557880098 26.84648033283169 24.654840992200278 106-107 22.549086577814748 26.092011557106286 26.60941424364624 24.74948762143273 108-109 22.602661959080464 25.94807433565845 26.561631913480383 24.887631791780702 110-111 22.33002714305898 26.286544158413626 26.709287626430182 24.674141072097207 112-113 22.24290153894216 26.244943825463768 26.908127414432627 24.604027221161445 114-115 22.288992868316814 26.328686898358946 26.520162916646267 24.862157316677973 116-117 22.047537260663663 26.253529414644643 26.76079060753142 24.938142717160268 118-119 21.96483816744418 26.455210744851737 26.375524268472084 25.204426819231994 120-121 22.05040927519473 26.68614152925076 26.425422389253235 24.838026806301276 122-123 21.95847413014498 26.81411650165832 26.288969845274657 24.93843952292204 124-125 22.000995032762876 26.6608397722149 26.324818047488336 25.01334714753389 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 39.0 1 43.0 2 95.0 3 133.5 4 88.0 5 71.0 6 165.0 7 409.0 8 714.0 9 848.5 10 708.5 11 526.0 12 466.0 13 529.5 14 641.0 15 701.0 16 773.5 17 908.0 18 1072.0 19 1164.5 20 1295.5 21 1588.5 22 1924.0 23 2452.0 24 3430.5 25 4804.0 26 6671.0 27 9128.5 28 12093.5 29 15821.5 30 20332.0 31 25540.5 32 32060.5 33 36359.0 34 39677.0 35 43776.5 36 48890.0 37 54788.0 38 59126.5 39 62095.0 40 62977.0 41 65636.0 42 68776.0 43 73148.0 44 78864.0 45 82725.5 46 83222.0 47 83012.5 48 83584.0 49 82442.5 50 81525.0 51 79746.5 52 79001.0 53 78976.5 54 78706.5 55 74231.5 56 66085.5 57 60090.0 58 56276.0 59 51393.0 60 42661.0 61 34098.5 62 26970.0 63 20067.5 64 15431.5 65 12032.5 66 8765.0 67 7358.5 68 5418.0 69 2992.5 70 1923.5 71 1362.0 72 1012.0 73 734.5 74 557.0 75 455.5 76 262.0 77 89.0 78 34.5 79 24.0 80 12.0 81 6.5 82 6.5 83 4.0 84 4.0 85 6.0 86 5.0 87 4.0 88 4.5 89 2.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029328726531773174 2 0.0 3 0.0 4 0.0 5 0.05478736673264464 6 0.014361284215876213 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.7976527693265353E-4 22-23 0.0 24-25 2.3313773077721127E-5 26-27 3.0307905001037463E-4 28-29 2.7976527693265353E-4 30-31 2.0982395769949012E-4 32-33 2.0982395769949012E-4 34-35 2.3313773077721127E-4 36-37 0.0 38-39 0.0 40-41 1.6319641154404786E-4 42-43 0.0 44-45 2.0982395769949012E-4 46-47 0.0 48-49 6.294718730984704E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.6319641154404786E-4 58-59 2.3313773077721127E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.994131923316338E-5 70-71 2.3313773077721127E-5 72-73 1.6319641154404786E-4 74-75 0.0 76-77 4.6627546155442255E-5 78-79 0.0 80-81 2.0982395769949012E-4 82-83 0.0 84-85 2.3313773077721127E-5 86-87 0.0 88-89 0.0 90-91 2.3313773077721127E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.0982395769949012E-4 100-101 0.0 102-103 0.0 104-105 0.0012822575192746619 106-107 2.3313773077721127E-5 108-109 0.0014687677038964309 110-111 0.004289734246300687 112-113 0.0 114-115 0.0 116-117 2.3313773077721127E-5 118-119 0.0 120-121 4.6627546155442255E-4 122-123 7.460407384870761E-4 124-125 9.325509231088451E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2144655.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.17068867437136 #Duplication Level Percentage of deduplicated Percentage of total 1 79.91723155500856 48.08674859614067 2 11.24557358919981 13.533078148009492 3 3.7777412507082184 6.819278780660835 4 1.673243733188156 4.027209109840297 5 0.9177020520919246 2.760938223612746 6 0.5523138766507092 1.993986379349098 7 0.35906252485724854 1.5123527578473461 8 0.24357959101082816 1.1725081390514596 9 0.17933198611292334 0.9711476195181658 >10 1.0115981001545258 11.215420017359978 >50 0.0880193836651487 3.6470553989701324 >100 0.03347401964686421 3.4056189177145995 >500 5.641688524924617E-4 0.20978892740756575 >1k 5.641688524924617E-4 0.6448689845176467 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2333 0.10878206518064677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2167 0.10104189251884335 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.662754615544225E-5 0.0 0.0 0.0 0.0 12-13 4.662754615544225E-5 0.0 0.0 0.0 0.0 14-15 4.662754615544225E-5 0.0 0.0 0.0 0.0 16-17 4.662754615544225E-5 0.0 0.0 9.32550923108845E-5 0.0 18-19 4.662754615544225E-5 0.0 0.0 9.32550923108845E-5 0.0 20-21 4.662754615544225E-5 0.0 0.0 1.6319641154404786E-4 0.0 22-23 4.662754615544225E-5 0.0 0.0 3.2639282308809573E-4 0.0 24-25 4.662754615544225E-5 0.0 0.0 7.46040738487076E-4 0.0 26-27 4.662754615544225E-5 0.0 0.0 0.001305571292352383 0.0 28-29 4.662754615544225E-5 0.0 0.0 0.0036602623732022166 0.0 30-31 4.662754615544225E-5 0.0 0.0 0.013452047065845088 0.0 32-33 4.662754615544225E-5 0.0 0.0 0.03392153982808424 0.0 34-35 4.662754615544225E-5 0.0 0.0 0.06383311068680043 0.0 36-37 4.662754615544225E-5 0.0 0.0 0.11794437800019118 0.0 38-39 9.32550923108845E-5 0.0 0.0 0.2025966880453966 0.0 40-41 9.32550923108845E-5 0.0 0.0 0.2957818390370479 0.0 42-43 9.32550923108845E-5 0.0 0.0 0.3865889851747717 0.0 44-45 9.32550923108845E-5 0.0 0.0 0.4979588791670455 2.3313773077721124E-5 46-47 9.32550923108845E-5 0.0 0.0 0.6371654182141184 4.662754615544225E-5 48-49 9.32550923108845E-5 0.0 0.0 0.7877957060692745 4.662754615544225E-5 50-51 9.32550923108845E-5 0.0 0.0 0.9429255521284309 4.662754615544225E-5 52-53 9.32550923108845E-5 0.0 0.0 1.0950012939144058 4.662754615544225E-5 54-55 9.32550923108845E-5 0.0 0.0 1.263629814585563 4.662754615544225E-5 56-57 9.32550923108845E-5 0.0 0.0 1.4574372101806583 4.662754615544225E-5 58-59 9.32550923108845E-5 0.0 0.0 1.6665151271416616 4.662754615544225E-5 60-61 9.32550923108845E-5 0.0 0.0 1.901284822034313 4.662754615544225E-5 62-63 9.32550923108845E-5 0.0 0.0 2.1321378030499076 4.662754615544225E-5 64-65 9.32550923108845E-5 0.0 0.0 2.371989900473503 4.662754615544225E-5 66-67 9.32550923108845E-5 0.0 0.0 2.6435487292827986 4.662754615544225E-5 68-69 9.32550923108845E-5 0.0 0.0 2.95238628124337 4.662754615544225E-5 70-71 9.32550923108845E-5 0.0 0.0 3.288477633931798 4.662754615544225E-5 72-73 9.32550923108845E-5 0.0 0.0 3.6311667844012208 4.662754615544225E-5 74-75 9.32550923108845E-5 0.0 0.0 3.9628751477510367 4.662754615544225E-5 76-77 9.32550923108845E-5 0.0 0.0 4.331815606705974 4.662754615544225E-5 78-79 9.32550923108845E-5 0.0 0.0 4.74024959725457 4.662754615544225E-5 80-81 9.32550923108845E-5 0.0 0.0 5.181043104835043 4.662754615544225E-5 82-83 9.32550923108845E-5 0.0 0.0 5.644311089662439 4.662754615544225E-5 84-85 9.32550923108845E-5 0.0 0.0 6.101004590481919 4.662754615544225E-5 86-87 9.32550923108845E-5 0.0 0.0 6.566464069978621 4.662754615544225E-5 88-89 9.32550923108845E-5 0.0 0.0 7.062394650887905 4.662754615544225E-5 90-91 9.32550923108845E-5 0.0 0.0 7.615513917156839 4.662754615544225E-5 92-93 1.3988263846632676E-4 0.0 0.0 8.179334205268447 4.662754615544225E-5 94-95 1.3988263846632676E-4 0.0 0.0 8.756956247042066 4.662754615544225E-5 96-97 1.3988263846632676E-4 0.0 0.0 9.336023742746502 4.662754615544225E-5 98-99 1.86510184621769E-4 0.0 0.0 9.940573192424889 4.662754615544225E-5 100-101 1.86510184621769E-4 0.0 0.0 10.60923551806701 4.662754615544225E-5 102-103 1.86510184621769E-4 0.0 0.0 11.308928475675575 4.662754615544225E-5 104-105 1.86510184621769E-4 0.0 0.0 12.008854571014918 4.662754615544225E-5 106-107 1.86510184621769E-4 0.0 0.0 12.696680818126925 4.662754615544225E-5 108-109 1.86510184621769E-4 0.0 0.0 13.411108080320611 4.662754615544225E-5 110-111 1.86510184621769E-4 0.0 0.0 14.162207907565552 4.662754615544225E-5 112-113 1.86510184621769E-4 0.0 0.0 14.94363895358461 4.662754615544225E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3015 0.0 60.644573 1 GTATCAA 4835 0.0 53.95337 1 TCAACGC 6145 0.0 41.862164 4 ATCAACG 6180 0.0 41.625084 3 CAACGCA 6375 0.0 40.35185 5 AACGCAG 6560 0.0 39.276237 6 TATCAAC 6660 0.0 38.982716 2 GTACATG 8525 0.0 34.58213 1 TACATGG 8700 0.0 33.127293 2 ACATGGG 8670 0.0 32.76115 3 ACGCAGA 7935 0.0 32.31659 7 CGCAGAG 8085 0.0 31.790613 8 ACCGTAT 130 6.382982E-5 27.46016 8 GCAGAGT 9555 0.0 26.83748 9 CATGGGG 4955 0.0 26.198273 4 GAGTACT 4710 0.0 24.56936 12-13 CATGGGA 4920 0.0 24.569187 4 AGAGTAC 8920 0.0 24.545855 10-11 GTGGTAT 1910 0.0 24.32216 1 CAGAGTA 9030 0.0 23.818573 10-11 >>END_MODULE