FastQCFastQC Report
Thu 26 May 2016
SRR1364360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714260
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.046.995531
GTATCAA20000.044.9548761
GTACATG62700.039.220431
ATCAACG21550.039.2187233
TCAACGC22300.038.166614
CAACGCA22350.038.0812265
TACATGG65100.037.5773282
ACATGGG64900.037.0500763
AACGCAG24150.035.4810456
TATCAAC24850.034.2511182
TAAGGTG11500.033.123375
CATGGGG39200.030.9739444
ACGCAGA29500.028.840437
AGGTAAG11350.028.3180262
GTATAGG6550.028.18061
GGTAAGG12850.027.7907683
GTAAGGT12300.027.581774
CATGGGA32200.027.1715164
CGCAGAG31600.026.923038
CCGTTGC2256.561095E-926.441899
Produced by FastQC (version 0.11.5)