##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364360_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1714260 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.490413356200342 31.0 31.0 34.0 26.0 34.0 2 30.477383827424077 31.0 31.0 34.0 26.0 34.0 3 30.649421907995286 31.0 31.0 34.0 26.0 34.0 4 34.087943485818954 37.0 35.0 37.0 30.0 37.0 5 34.140971031232134 37.0 35.0 37.0 30.0 37.0 6 34.18470535391364 37.0 35.0 37.0 30.0 37.0 7 34.14231388470827 37.0 35.0 37.0 30.0 37.0 8 34.0250230420123 37.0 35.0 37.0 30.0 37.0 9 35.51720334138346 39.0 37.0 39.0 30.0 39.0 10-11 35.429490859029556 39.0 36.0 39.0 29.5 39.0 12-13 35.41307823784024 39.0 35.0 39.0 29.0 39.0 14-15 36.643643904658575 40.0 37.0 41.0 29.0 41.0 16-17 36.62903235215195 40.0 37.0 41.0 29.0 41.0 18-19 36.607282442569975 40.0 37.0 41.0 29.0 41.0 20-21 36.62544421499656 40.0 37.0 41.0 29.0 41.0 22-23 36.58121988496494 40.0 37.0 41.0 29.0 41.0 24-25 36.56853569470209 40.0 37.0 41.0 29.0 41.0 26-27 36.500476590482194 40.0 37.0 41.0 29.0 41.0 28-29 36.49630715294063 40.0 36.0 41.0 29.0 41.0 30-31 36.38497369127204 40.0 36.0 41.0 29.0 41.0 32-33 36.340486857302864 40.0 36.0 41.0 29.0 41.0 34-35 36.320448181722725 40.0 36.0 41.0 29.0 41.0 36-37 36.24651978113005 40.0 36.0 41.0 29.0 41.0 38-39 36.130050867429674 40.0 36.0 41.0 28.0 41.0 40-41 36.05542858142872 40.0 36.0 41.0 28.0 41.0 42-43 35.902740541108116 40.0 35.0 41.0 27.0 41.0 44-45 35.74421207984787 40.0 35.0 41.0 27.0 41.0 46-47 35.61441379954033 39.5 35.0 41.0 27.0 41.0 48-49 35.43754360482074 39.0 35.0 41.0 27.0 41.0 50-51 35.286935762369765 39.0 35.0 41.0 26.0 41.0 52-53 35.102284659269884 39.0 34.5 41.0 26.0 41.0 54-55 34.901158517377766 39.0 34.0 41.0 26.0 41.0 56-57 34.690827237408556 38.0 34.0 40.0 26.0 41.0 58-59 34.443206106424924 38.0 34.0 40.0 25.0 41.0 60-61 34.180140702110535 37.5 33.5 40.0 24.5 41.0 62-63 33.89715999906665 37.0 33.0 40.0 23.5 41.0 64-65 33.54392653389801 36.5 33.0 40.0 23.0 41.0 66-67 33.22992544888173 36.0 33.0 39.0 22.0 41.0 68-69 32.90824233196831 35.5 32.0 39.0 22.0 41.0 70-71 32.5479717195758 35.0 32.0 38.5 21.5 40.0 72-73 32.20506486763968 35.0 32.0 37.5 20.0 40.0 74-75 31.8657024022027 35.0 32.0 37.0 20.0 39.0 76-77 31.656329261605592 35.0 31.5 37.0 20.0 39.0 78-79 31.518867324676535 35.0 32.0 36.0 20.0 39.0 80-81 31.283429001435024 35.0 32.0 36.0 19.5 37.5 82-83 31.006608682463572 35.0 32.0 35.5 18.0 37.0 84-85 30.77801383687422 35.0 31.0 35.0 18.0 37.0 86-87 30.596852286117624 35.0 31.0 35.0 17.0 36.0 88-89 30.358870883063247 35.0 31.0 35.0 13.0 36.0 90-91 30.241321619824298 35.0 31.0 35.0 11.5 36.0 92-93 30.108831799143655 35.0 31.0 35.0 9.0 35.5 94-95 30.013098363142113 34.0 31.0 35.0 7.0 35.0 96-97 29.919305414581217 34.0 31.0 35.0 7.0 35.0 98-99 29.791847794383585 34.0 31.0 35.0 6.0 35.0 100-101 29.7027711665675 34.0 31.0 35.0 2.0 35.0 102-103 28.66736405212745 33.5 29.0 34.5 2.0 35.0 104-105 29.402368368858866 34.0 30.0 35.0 2.0 35.0 106-107 29.774784163429118 34.5 31.0 35.0 2.0 35.0 108-109 29.829327523246185 35.0 31.0 35.0 2.0 35.0 110-111 29.765189936182377 35.0 31.0 35.0 2.0 35.0 112-113 29.675530841295952 35.0 31.0 35.0 2.0 35.0 114-115 29.580047075706133 35.0 31.0 35.0 2.0 35.0 116-117 29.45991535706369 35.0 31.0 35.0 2.0 35.0 118-119 29.285792995228263 35.0 31.0 35.0 2.0 35.0 120-121 29.113968417859603 35.0 31.0 35.0 2.0 35.0 122-123 28.938672371752244 35.0 31.0 35.0 2.0 35.0 124-125 28.099332364985475 34.5 28.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.7955089841237282E-4 1101 2 -0.15260448723682174 1101 3 -0.009813844517410075 1101 4 0.025430404754537506 1101 5 0.018237160848762812 1101 6 -0.057938730429413 1101 7 -0.003632946473594245 1101 8 -0.2223948061933001 1101 9 0.0511776784567175 1101 10-11 0.049725115890140614 1101 12-13 0.1734763635780041 1101 14-15 0.2045553168326819 1101 16-17 0.11151525983623145 1101 18-19 0.010335149030943569 1101 20-21 0.09186505017459723 1101 22-23 0.3339087734081403 1101 24-25 0.26716583473123734 1101 26-27 0.4392485768467509 1101 28-29 0.28103453332374073 1101 30-31 0.34119207522545736 1101 32-33 0.2629396145205902 1101 34-35 0.31668473293142796 1101 36-37 0.22324654344961914 1101 38-39 0.2983482262782573 1101 40-41 0.14575539210822797 1101 42-43 0.25678638537087295 1101 44-45 0.28404139668528927 1101 46-47 0.2389671173392216 1101 48-49 0.13948482401121254 1101 50-51 0.16838956600155797 1101 52-53 0.09036859071049719 1101 54-55 0.30252945389407415 1101 56-57 0.08253243832596979 1101 58-59 0.15183375191141124 1101 60-61 0.2070034915671144 1101 62-63 0.19741800309594737 1101 64-65 0.20872433476712615 1101 66-67 0.14465013683973638 1101 68-69 0.13411156918960643 1101 70-71 0.14061915738800224 1101 72-73 0.20241261558643941 1101 74-75 0.13589876296023817 1101 76-77 0.17539577394021322 1101 78-79 0.12122441846557663 1101 80-81 0.2879156416595663 1101 82-83 0.14465601729468602 1101 84-85 0.16564688836344743 1101 86-87 0.11039039333594403 1101 88-89 0.16192818764192296 1101 90-91 0.07159755467028006 1101 92-93 0.05626750100701017 1101 94-95 0.04612415608887588 1101 96-97 -0.05073027076204184 1101 98-99 -0.06337304087652029 1101 100-101 0.030121009546224542 1101 102-103 -0.19921367119508915 1101 104-105 -0.088669986300836 1101 106-107 -0.04984800351927987 1101 108-109 -0.10604610078853227 1101 110-111 -0.05925902837139674 1101 112-113 -0.03618349112510799 1101 114-115 -0.10794523945693868 1101 116-117 -0.11049478606932794 1101 118-119 -0.14794683014596188 1101 120-121 -0.27032653651181704 1101 122-123 -0.3069746389311412 1101 124-125 -0.37248130170554816 1102 1 0.32569993366568895 1102 2 0.2964783154103152 1102 3 0.3803427926045764 1102 4 0.46292047278973314 1102 5 0.46014723043484196 1102 6 0.4539858216628758 1102 7 0.4891731373719921 1102 8 0.4213333998831743 1102 9 0.48772712537915197 1102 10-11 0.5368977204761194 1102 12-13 0.4498729068030656 1102 14-15 0.40578262090703276 1102 16-17 0.5413689374277126 1102 18-19 0.5502445557467937 1102 20-21 0.6320699495717292 1102 22-23 0.5639103655814353 1102 24-25 0.6283159344600193 1102 26-27 0.5972441698456521 1102 28-29 0.5303732307975153 1102 30-31 0.6960874269206272 1102 32-33 0.7782854273724809 1102 34-35 0.71465999627857 1102 36-37 0.4835849253947089 1102 38-39 0.6096903733078918 1102 40-41 0.5499824210317925 1102 42-43 0.5071026405425698 1102 44-45 0.46444862597797254 1102 46-47 0.46389080849553466 1102 48-49 0.45873965937728656 1102 50-51 0.39227635620924417 1102 52-53 0.42953859275845474 1102 54-55 0.5155953541151348 1102 56-57 0.3089746590299427 1102 58-59 0.23865124526167847 1102 60-61 0.27636137465265165 1102 62-63 0.34792648145699445 1102 64-65 0.3348397546366755 1102 66-67 0.38379173671848577 1102 68-69 0.4227266277230086 1102 70-71 0.3729069177048956 1102 72-73 0.30629725842496214 1102 74-75 0.2798268701414841 1102 76-77 0.40534972327177954 1102 78-79 0.4618192703162194 1102 80-81 0.4375728196769302 1102 82-83 0.47648175675670856 1102 84-85 0.3926329869010807 1102 86-87 0.4261253174354671 1102 88-89 0.4445521779463313 1102 90-91 0.44917196965365136 1102 92-93 0.3535076727781892 1102 94-95 0.26076555843718197 1102 96-97 0.21852481696578963 1102 98-99 0.26539980952511044 1102 100-101 0.14229940698174204 1102 102-103 -0.10723117378332603 1102 104-105 0.15027400667096558 1102 106-107 0.21700261192987824 1102 108-109 0.28989344434545217 1102 110-111 0.2001279497310975 1102 112-113 0.2572694953316308 1102 114-115 0.24334921578807922 1102 116-117 0.13446168764459543 1102 118-119 0.3043480788913868 1102 120-121 0.11602058510345614 1102 122-123 0.12372865659228438 1102 124-125 0.05262613940277561 1103 1 0.4139434138460487 1103 2 0.4542136524518625 1103 3 0.49312620988563083 1103 4 0.44778304026432636 1103 5 0.4418102470702294 1103 6 0.40070826152733474 1103 7 0.28188435181568394 1103 8 0.47437282322022156 1103 9 0.5577795334700468 1103 10-11 0.6659514446177397 1103 12-13 0.5321737641555302 1103 14-15 0.6846759776101692 1103 16-17 0.5739129252533459 1103 18-19 0.647522913070766 1103 20-21 0.6418364075537824 1103 22-23 0.7070473020385037 1103 24-25 0.757387578845595 1103 26-27 0.850451668575495 1103 28-29 0.765474309971097 1103 30-31 0.8982872380907452 1103 32-33 0.8573801356369017 1103 34-35 0.8470937483607699 1103 36-37 0.899077403690967 1103 38-39 0.8504547744358248 1103 40-41 0.7732404683460388 1103 42-43 0.7545884828000311 1103 44-45 0.6561670439098535 1103 46-47 0.7675257237890136 1103 48-49 0.8798926853641831 1103 50-51 0.7716279013091736 1103 52-53 0.8141697116594955 1103 54-55 0.785955975406246 1103 56-57 0.6443884652057932 1103 58-59 0.6684446780846045 1103 60-61 0.5232073367343162 1103 62-63 0.5307758032469891 1103 64-65 0.5076999275467884 1103 66-67 0.5629860035600629 1103 68-69 0.39133560889939645 1103 70-71 0.4898695758154332 1103 72-73 0.5486945761768283 1103 74-75 0.44842884817651907 1103 76-77 0.48094735965907986 1103 78-79 0.5643249403114012 1103 80-81 0.5189017849695432 1103 82-83 0.48832433873545256 1103 84-85 0.44329471627810335 1103 86-87 0.3845034138886696 1103 88-89 0.5659293644410397 1103 90-91 0.48522592593365843 1103 92-93 0.47486139050150555 1103 94-95 0.45364451836121944 1103 96-97 0.4756709453833068 1103 98-99 0.43534865510149245 1103 100-101 0.4541034042008789 1103 102-103 0.2125686263497144 1103 104-105 0.39234433620550746 1103 106-107 0.5258482692924176 1103 108-109 0.49686753842088294 1103 110-111 0.42219853719718614 1103 112-113 0.4681972900948992 1103 114-115 0.34819029096672693 1103 116-117 0.24868554763215656 1103 118-119 0.23348243663284407 1103 120-121 0.29700432701834956 1103 122-123 0.23899773917776557 1103 124-125 0.07718542136934303 1104 1 0.3708999451184418 1104 2 0.45321311121329444 1104 3 0.4303001251869887 1104 4 0.5628819230509805 1104 5 0.45157994948133506 1104 6 0.3768891483046062 1104 7 0.5098629238459935 1104 8 0.40200495640414147 1104 9 0.6320226938416837 1104 10-11 0.5078588605093728 1104 12-13 0.501681202435293 1104 14-15 0.5358383999585925 1104 16-17 0.6034977006393376 1104 18-19 0.614769468589266 1104 20-21 0.591073365031356 1104 22-23 0.6537539114962101 1104 24-25 0.5988346822960224 1104 26-27 0.5684528133700795 1104 28-29 0.6610393561148626 1104 30-31 0.6690015864521968 1104 32-33 0.579864963069511 1104 34-35 0.5542568504309813 1104 36-37 0.5196443539789541 1104 38-39 0.49935827586855197 1104 40-41 0.6122814259113127 1104 42-43 0.4865180098452271 1104 44-45 0.4500451625230397 1104 46-47 0.5079051793422522 1104 48-49 0.4209535360435055 1104 50-51 0.3721755158780695 1104 52-53 0.24041124254529223 1104 54-55 0.29614646484055385 1104 56-57 0.2769583040228696 1104 58-59 0.23887262584412383 1104 60-61 0.19374177231711798 1104 62-63 0.267238203322691 1104 64-65 0.4255923630164915 1104 66-67 0.3069687635536482 1104 68-69 0.2490755512672962 1104 70-71 0.09378651589027953 1104 72-73 0.15478512573592695 1104 74-75 0.05944781345300498 1104 76-77 0.005876358160005424 1104 78-79 0.15228154544151806 1104 80-81 0.14146433610735443 1104 82-83 0.18011883310437682 1104 84-85 0.02900962434256016 1104 86-87 0.09503021558416691 1104 88-89 0.0643570029213727 1104 90-91 0.0895131643590581 1104 92-93 0.05862541625477036 1104 94-95 0.15329288639421534 1104 96-97 0.10326058153019346 1104 98-99 0.10690252911177112 1104 100-101 0.08683235446347481 1104 102-103 0.04794569306746865 1104 104-105 0.12653662130552235 1104 106-107 0.1645182937924048 1104 108-109 0.17491988161460625 1104 110-111 0.17267904510746135 1104 112-113 0.26909867158038736 1104 114-115 0.26232175308406624 1104 116-117 0.21112962060819385 1104 118-119 0.19886797967746617 1104 120-121 0.15561022224887466 1104 122-123 0.05884333616160475 1104 124-125 -3.70084434834439E-4 1105 1 0.24427067832338878 1105 2 0.328858952612304 1105 3 0.27870822648443294 1105 4 0.494104045514419 1105 5 0.32999900213766864 1105 6 0.3122207246674691 1105 7 0.39184593500819886 1105 8 0.4231531274885185 1105 9 0.34652134792714406 1105 10-11 0.3620291368688413 1105 12-13 0.49276944087480956 1105 14-15 0.5257081857314461 1105 16-17 0.637820317779223 1105 18-19 0.4966054668085249 1105 20-21 0.540858634942424 1105 22-23 0.5541959589664742 1105 24-25 0.54156581800693 1105 26-27 0.5508081513906902 1105 28-29 0.6078478211864393 1105 30-31 0.6365285137241798 1105 32-33 0.7139606635212346 1105 34-35 0.5512318242408369 1105 36-37 0.46596355887106 1105 38-39 0.524733483572696 1105 40-41 0.5190774308069521 1105 42-43 0.49223154904642286 1105 44-45 0.46422002889186587 1105 46-47 0.5979141990837107 1105 48-49 0.4737092135203227 1105 50-51 0.42843843305147544 1105 52-53 0.5045339497167873 1105 54-55 0.40328567034612917 1105 56-57 0.44145431906018473 1105 58-59 0.3788652862505799 1105 60-61 0.3499383954961388 1105 62-63 0.4124807042118164 1105 64-65 0.3776158497158306 1105 66-67 0.3313602695216247 1105 68-69 0.17355580386427505 1105 70-71 0.3398267001649202 1105 72-73 0.2551214592939317 1105 74-75 0.16892755067566156 1105 76-77 0.15527183651718346 1105 78-79 0.3868818705832986 1105 80-81 0.2910595392850439 1105 82-83 0.3528021149969973 1105 84-85 0.35925458051363535 1105 86-87 0.27701904370586306 1105 88-89 0.34259917026797027 1105 90-91 0.230825556085545 1105 92-93 0.2480171198059331 1105 94-95 0.16660467348852492 1105 96-97 0.16898151559295727 1105 98-99 0.19624024962082132 1105 100-101 0.09291985092977484 1105 102-103 -0.10570424866621764 1105 104-105 0.10132880662804666 1105 106-107 0.11563801013480202 1105 108-109 0.14274435810897756 1105 110-111 0.10847646182476822 1105 112-113 0.16326696934460472 1105 114-115 0.11483219252288634 1105 116-117 0.041271640085199124 1105 118-119 0.10284718066212051 1105 120-121 -0.18892469595030548 1105 122-123 -0.23510973410146718 1105 124-125 -0.39277298335321476 1106 1 0.16272276269475583 1106 2 0.15043804624663082 1106 3 0.2047303503709621 1106 4 0.2178399467577563 1106 5 0.0943962191388863 1106 6 0.06741212572087818 1106 7 0.0601561890530391 1106 8 0.13203425476417863 1106 9 0.21267175220523882 1106 10-11 0.17306962058836461 1106 12-13 0.116224723622814 1106 14-15 0.2005226963304807 1106 16-17 0.17651944180223467 1106 18-19 0.1612038773222224 1106 20-21 0.10695485592475507 1106 22-23 0.13532331684426424 1106 24-25 0.14060737388150102 1106 26-27 0.2530230477682238 1106 28-29 0.22063211904821145 1106 30-31 0.24748875025605344 1106 32-33 0.16774370998732024 1106 34-35 0.14803061099545545 1106 36-37 0.17003992689650005 1106 38-39 0.05292606366777619 1106 40-41 0.10074723829593069 1106 42-43 0.08724224593075292 1106 44-45 0.062200940585427134 1106 46-47 0.09462990204414012 1106 48-49 -0.01354784551892152 1106 50-51 -0.13112660416973654 1106 52-53 0.05276037176108872 1106 54-55 0.03367748381746338 1106 56-57 0.025436783076393965 1106 58-59 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2216 28-29 -1.8893915267685557 2216 30-31 -1.8572690990648653 2216 32-33 -1.7638016802699354 2216 34-35 -1.4157981257317047 2216 36-37 -1.5176122532072895 2216 38-39 -1.8756983026642544 2216 40-41 -1.5319267794852038 2216 42-43 -1.5025308340604937 2216 44-45 -1.6173066437700854 2216 46-47 -1.5550758508180493 2216 48-49 -1.5962169035765754 2216 50-51 -1.555887301235778 2216 52-53 -1.5415139923964105 2216 54-55 -1.3405665679400087 2216 56-57 -1.5574853251367244 2216 58-59 -1.5637131219821683 2216 60-61 -1.6106691306477288 2216 62-63 -1.6442069693383132 2216 64-65 -1.5237099677337227 2216 66-67 -1.7223545346677085 2216 68-69 -1.6440181283661985 2216 70-71 -1.6077071723933116 2216 72-73 -1.5489171151494077 2216 74-75 -1.405685543688115 2216 76-77 -1.537150733758974 2216 78-79 -1.7080895436565697 2216 80-81 -1.4786303764316528 2216 82-83 -1.506207978917935 2216 84-85 -1.5289641868925337 2216 86-87 -1.527428180610034 2216 88-89 -1.5747183619369558 2216 90-91 -1.767019970462595 2216 92-93 -1.7554893621030558 2216 94-95 -1.6849971068569083 2216 96-97 -1.5567471885382496 2216 98-99 -1.6018062549100627 2216 100-101 -1.6170314750119026 2216 102-103 -1.6928750688437582 2216 104-105 -1.8463797944432976 2216 106-107 -1.6833663223430868 2216 108-109 -1.5901358649472073 2216 110-111 -1.5307029470155449 2216 112-113 -1.5761573517244116 2216 114-115 -1.5794255752227997 2216 116-117 -1.733778882105323 2216 118-119 -1.694834885936995 2216 120-121 -1.5222247789810623 2216 122-123 -1.648921295940525 2216 124-125 -1.5902192724221393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43830.0 3 7839.0 4 5020.0 5 4081.0 6 4262.0 7 4792.0 8 5431.0 9 5990.0 10 6530.0 11 7196.0 12 7663.0 13 7767.0 14 7719.0 15 6866.0 16 6504.0 17 5731.0 18 5597.0 19 5503.0 20 5720.0 21 5878.0 22 6950.0 23 8947.0 24 12825.0 25 17222.0 26 20188.0 27 21645.0 28 25340.0 29 30249.0 30 35758.0 31 44160.0 32 55881.0 33 73943.0 34 101187.0 35 154824.0 36 296373.0 37 479604.0 38 168985.0 39 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.586672704915784 17.989975028027086 12.251731960098535 25.171620306958598 2 15.896941654993855 19.769592969359376 38.55464422375163 25.778821151895137 3 18.69897072240773 23.936945955342747 29.308393594257886 28.05568972799164 4 12.485510285873966 15.702737279824893 36.79491898886151 35.01683344543963 5 13.302704383763544 38.538737705291084 34.49578637999775 13.662771530947628 6 33.47967855400652 36.23024514575003 16.875447846717968 13.414628453525474 7 29.441210682963426 32.154711860768195 20.838977345779913 17.565100110488473 8 27.225100043167316 34.709845647684716 19.713345700185503 18.351708608962465 9 26.9000035000525 15.463115280062533 19.03159380724044 38.60528741264452 10-11 25.164816158123177 26.309275993089727 27.69468548646543 20.831222362321668 12-13 25.61437629594715 23.67541039359459 27.508861806568923 23.201351503889338 14-15 22.55555192585454 25.33513159092129 24.48242732107065 27.626889162153525 16-17 23.1788330578011 28.497985717435764 25.594863783631165 22.728317441131964 18-19 23.372008913467035 27.28891766709834 26.57487778983351 22.76419562960111 20-21 23.430298455484262 26.796505078870815 26.65577371914046 23.117422746504467 22-23 23.51104849906082 26.61883261582257 26.507997619964303 23.36212126515231 24-25 23.278820015633567 26.938037403894395 26.4915473732106 23.29159520726144 26-27 22.879944045794716 27.204523937164687 26.313905891700145 23.601626125340452 28-29 23.19227489041881 27.27810464493593 26.28326957382045 23.246350890824814 30-31 23.000738511939275 26.818250228378695 26.301904380787487 23.879106878894543 32-33 22.686462597812 27.110009111813977 26.22291977053629 23.980608519837737 34-35 23.746198768322184 27.01457257909741 25.901197435375416 23.33803121720499 36-37 23.815253067142443 26.44755735497301 26.019850565127722 23.717339012756828 38-39 23.18964519020345 26.753901764409232 26.160816657250535 23.895636388136783 40-41 23.50442744253104 26.52058347331182 26.397672686884043 23.577316397273094 42-43 23.178569199349216 26.578874756962694 26.2083905257306 24.03416551795749 44-45 23.153173885790803 26.76318805248883 26.535068338202862 23.548569723517506 46-47 23.825032375485634 27.159824064027628 25.68318108104955 23.33196247943719 48-49 23.34514601052349 27.053130796961955 25.847508545961524 23.75421464655303 50-51 23.403596887286643 27.04298064470967 26.138712914027046 23.414709553976643 52-53 23.725883957152963 26.61795696337293 26.153873077680313 23.502286001793802 54-55 22.960134755755192 26.863588965778746 26.38115169502031 23.79512458344575 56-57 23.089983220150287 27.261699446145375 26.176448884459376 23.471868449244965 58-59 23.10306376949146 26.5871058650035 26.250485272083417 24.059345093421623 60-61 22.632731676484923 26.819196978716786 26.702993778286977 23.84507756651131 62-63 22.96596374323281 26.81269981458315 26.80672050765191 23.414615934532133 64-65 22.783550379529444 26.88256754644073 26.3813506721393 23.952531401890532 66-67 22.596638623598004 27.12164243731145 26.402243367711687 23.879475571378862 68-69 23.14740679098716 26.45764186586326 26.78712529499938 23.607826048150198 70-71 22.966148931633718 26.47238890708625 26.431933413816527 24.12952874746351 72-73 22.771366668766756 26.711981948557124 26.343066085174993 24.173585297501127 74-75 22.849650936063036 27.038009832417227 26.372783075758615 23.739556155761125 76-77 23.11420818692436 26.623881752398376 26.558604764170106 23.703305296507157 78-79 23.070746208753643 26.758942301185616 26.56940915126017 23.60090233880057 80-81 23.28198196805515 26.78864718412377 26.3949148177132 23.53445603010788 82-83 23.066606815297565 26.881781587420477 26.481693268733526 23.569918328548432 84-85 22.830132325935743 26.91229177507158 26.65836178111503 23.59921411787765 86-87 23.14590466656808 26.896869752550057 26.395185464222763 23.562040116659098 88-89 22.928580241674023 26.9674188972671 26.283501301068128 23.82049955999075 90-91 22.819353758580277 27.055693711130985 26.46549425959209 23.65945827069665 92-93 23.048671074445732 26.473362570745557 26.808816958165764 23.669149396642947 94-95 22.657063954946747 26.308803095019478 27.109596217094712 23.924536732939067 96-97 23.092123665346268 25.9997707459594 26.994692448121064 23.91341314057327 98-99 22.751016262830344 26.34431512620851 26.63403425353355 24.270634357427593 100-101 22.61609056469932 26.11875824718152 27.092523605460674 24.17262758265849 102-103 22.358898224132375 25.81324295274999 27.326514927234204 24.501343895883434 104-105 22.16232218848531 26.319750094064737 26.949706722201316 24.568220995248637 106-107 22.574572336814764 26.234481294129246 26.61790949519322 24.57303687386277 108-109 22.577102694018603 26.067604370141932 26.656559077623193 24.698733858216272 110-111 22.57246410629158 26.291543481963892 26.917364551273227 24.218627860471305 112-113 22.569644163447506 26.387837224523892 27.15915282455983 23.883365787468776 114-115 22.30713026281824 26.72199835784174 26.888139271354255 24.08273210798576 116-117 22.322176250887786 26.554449294363515 27.002054855452805 24.121319599295894 118-119 22.019783824699637 26.838483650579338 26.687766144792104 24.45396637992892 120-121 21.9503031158713 27.04554351700757 26.947587295679014 24.056566071442116 122-123 22.019378350360903 26.998840845861665 26.954213119851406 24.027567683926026 124-125 22.037119625344438 27.062307546692672 26.877208190690254 24.02336463727264 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 414.0 1 356.0 2 271.0 3 220.0 4 195.5 5 178.0 6 154.5 7 139.5 8 123.5 9 118.5 10 123.0 11 114.0 12 114.0 13 142.5 14 160.5 15 165.5 16 185.0 17 238.5 18 307.5 19 413.5 20 526.5 21 726.0 22 1057.0 23 1541.5 24 2394.0 25 3541.5 26 5113.0 27 7371.5 28 9849.5 29 12491.0 30 15661.5 31 20096.5 32 26874.5 33 29965.0 34 29397.0 35 31675.5 36 36489.0 37 41158.0 38 43926.5 39 46265.5 40 48604.0 41 51557.0 42 53478.0 43 54849.0 44 57579.0 45 62559.5 46 64366.5 47 63339.0 48 62406.5 49 60744.0 50 61314.5 51 61333.0 52 62225.5 53 65455.0 54 68657.0 55 66231.5 56 60415.5 57 56172.5 58 52800.0 59 46102.0 60 36094.5 61 29859.5 62 25749.5 63 19547.0 64 13680.5 65 10252.0 66 7612.5 67 6022.0 68 4638.0 69 3209.0 70 2285.5 71 1506.5 72 1081.5 73 738.0 74 501.5 75 431.0 76 307.5 77 164.0 78 107.0 79 68.0 80 39.0 81 28.0 82 19.0 83 12.5 84 10.0 85 7.0 86 5.0 87 5.5 88 7.0 89 6.0 90 4.0 91 5.0 92 4.0 93 2.0 94 2.5 95 2.0 96 1.5 97 1.0 98 0.5 99 2.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043108979968032854 2 0.00950847596047274 3 0.0028000420006300095 4 0.006591765543149814 5 0.024092028047087374 6 0.0294004410066151 7 0.0031500472507087606 8 0.0 9 0.0 10-11 8.75013125196878E-5 12-13 0.0012541854794488583 14-15 4.6667366677166823E-4 16-17 1.1666841669291706E-4 18-19 0.0 20-21 5.833420834645853E-5 22-23 0.0 24-25 0.0 26-27 5.833420834645853E-5 28-29 3.500052500787512E-4 30-31 1.1666841669291706E-4 32-33 1.1666841669291706E-4 34-35 7.583447085039609E-4 36-37 8.75013125196878E-5 38-39 2.0416972921260485E-4 40-41 4.958407709448975E-4 42-43 9.91681541889795E-4 44-45 0.0016625249378740682 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.001312519687795317 54-55 0.00277087489645678 56-57 8.75013125196878E-5 58-59 9.041802293701072E-4 60-61 0.0012541854794488583 62-63 8.458460210236487E-4 64-65 0.001866694667086673 66-67 0.0030042117298426144 68-69 0.0035875538133071996 70-71 0.0017791933545669852 72-73 0.0028000420006300095 74-75 0.001895861771259902 76-77 0.0015458565211811512 78-79 0.0028000420006300095 80-81 0.0014291881044882341 82-83 7.875118126771902E-4 84-85 4.6667366677166823E-4 86-87 0.0020125301879528193 88-89 0.005337580063700956 90-91 5.250078751181268E-4 92-93 0.0010500157502362536 94-95 0.0012833525836220877 96-97 2.625039375590634E-4 98-99 7.875118126771902E-4 100-101 0.0022166999171654243 102-103 0.0016041907295276095 104-105 8.750131251968779E-4 106-107 0.006941770793228564 108-109 0.0021291986046457364 110-111 0.001866694667086673 112-113 0.0026833735839370924 114-115 0.0030625459381890727 116-117 0.0016041907295276095 118-119 0.046054857489529005 120-121 0.013358533711339002 122-123 0.0046084024593702234 124-125 0.002654206479763863 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1714260.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.01557534132697 #Duplication Level Percentage of deduplicated Percentage of total 1 85.32720523397066 62.302149824258535 2 9.208544523209394 13.447343528367186 3 2.624172912368593 5.748164849751552 4 1.0630771018140857 3.1048474488458355 5 0.5438944716153726 1.9856383884981732 6 0.2865644079546001 1.2554199071491114 7 0.19213966394381884 0.9820431676123004 8 0.13799444191448781 0.8060594856233323 9 0.09691802563667042 0.6368872862526269 >10 0.4696216732127715 6.2373603534689055 >50 0.03562185967052102 1.7905400427730656 >100 0.01408739932430103 1.644141210940513 >500 1.5828536476489522E-4 0.05940450645880202 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 5.833420834645853E-5 14-15 0.0 0.0 0.0 0.0 5.833420834645853E-5 16-17 0.0 0.0 0.0 0.0 5.833420834645853E-5 18-19 0.0 0.0 0.0 0.0 5.833420834645853E-5 20-21 0.0 0.0 0.0 0.0 5.833420834645853E-5 22-23 0.0 0.0 0.0 2.625039375590634E-4 8.75013125196878E-5 24-25 0.0 0.0 0.0 3.500052500787512E-4 1.1666841669291706E-4 26-27 0.0 0.0 0.0 7.291776043307316E-4 1.1666841669291706E-4 28-29 0.0 0.0 0.0 0.0032958827715749072 1.1666841669291706E-4 30-31 0.0 0.0 0.0 0.014175212628189424 1.4583552086614633E-4 32-33 0.0 0.0 0.0 0.035583867091339705 2.3333683338583411E-4 34-35 0.0 0.0 0.0 0.06381762393102564 2.3333683338583411E-4 36-37 0.0 0.0 0.0 0.11360587075472799 2.3333683338583411E-4 38-39 5.833420834645853E-5 0.0 0.0 0.19282372568921868 2.9167104173229266E-4 40-41 8.75013125196878E-5 0.0 0.0 0.27691248702063864 2.9167104173229266E-4 42-43 1.1666841669291706E-4 0.0 0.0 0.3578220339971766 2.9167104173229266E-4 44-45 1.750026250393756E-4 0.0 0.0 0.46235693535403033 2.9167104173229266E-4 46-47 1.750026250393756E-4 0.0 0.0 0.5855004491734043 3.208381459055219E-4 48-49 1.750026250393756E-4 0.0 0.0 0.7129023602020697 3.500052500787512E-4 50-51 1.750026250393756E-4 0.0 0.0 0.8636379545693185 3.500052500787512E-4 52-53 1.750026250393756E-4 0.0 0.0 1.0057692532054647 4.3750656259843897E-4 54-55 1.750026250393756E-4 0.0 0.0 1.1578173672605088 4.6667366677166823E-4 56-57 1.750026250393756E-4 0.0 0.0 1.3359992066547663 4.6667366677166823E-4 58-59 1.750026250393756E-4 0.0 0.0 1.525877054822489 4.6667366677166823E-4 60-61 1.750026250393756E-4 0.0 0.0 1.7393219231621808 4.6667366677166823E-4 62-63 1.750026250393756E-4 0.0 0.0 1.9481583890425025 4.6667366677166823E-4 64-65 1.750026250393756E-4 0.0 0.0 2.1511030998798315 4.6667366677166823E-4 66-67 1.750026250393756E-4 0.0 0.0 2.394619252622123 4.6667366677166823E-4 68-69 1.750026250393756E-4 0.0 0.0 2.677598497310793 4.6667366677166823E-4 70-71 2.3333683338583411E-4 0.0 0.0 2.976852986128125 4.6667366677166823E-4 72-73 2.3333683338583411E-4 0.0 0.0 3.292091048032387 5.833420834645853E-4 74-75 2.3333683338583411E-4 0.0 0.0 3.5964206129758614 6.416762918110438E-4 76-77 2.3333683338583411E-4 0.0 0.0 3.920817145590517 6.416762918110438E-4 78-79 2.3333683338583411E-4 0.0 0.0 4.297510295987774 6.416762918110438E-4 80-81 2.3333683338583411E-4 0.0 0.0 4.697537129723612 6.416762918110438E-4 82-83 2.3333683338583411E-4 0.0 0.0 5.125418547944886 6.708433959842731E-4 84-85 2.3333683338583411E-4 0.0 0.0 5.539883098246474 7.000105001575024E-4 86-87 2.3333683338583411E-4 0.0 0.0 5.973947942552472 7.000105001575024E-4 88-89 2.3333683338583411E-4 0.0 0.0 6.428779765029809 7.000105001575024E-4 90-91 2.3333683338583411E-4 0.0 0.0 6.934333181664392 7.000105001575024E-4 92-93 2.3333683338583411E-4 0.0 0.0 7.451932612322518 7.000105001575024E-4 94-95 2.3333683338583411E-4 0.0 0.0 7.968569528542929 7.000105001575024E-4 96-97 2.3333683338583411E-4 0.0 0.0 8.489056502514204 7.875118126771902E-4 98-99 2.9167104173229266E-4 0.0 0.0 9.031447971719576 8.750131251968779E-4 100-101 3.208381459055219E-4 0.0 0.0 9.64792388552495 8.750131251968779E-4 102-103 3.500052500787512E-4 0.0 0.0 10.293887741649458 9.91681541889795E-4 104-105 3.500052500787512E-4 0.0 0.0 10.938859916232076 9.91681541889795E-4 106-107 3.500052500787512E-4 0.0 0.0 11.567206841435954 0.0010208486460630242 108-109 3.500052500787512E-4 0.0 0.0 12.220870813062195 0.0010500157502362536 110-111 3.500052500787512E-4 0.0 0.0 12.918956284344265 0.0010500157502362536 112-113 3.500052500787512E-4 0.0 0.0 13.650729760946415 0.0010500157502362536 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1305 0.0 46.99553 1 GTATCAA 2000 0.0 44.954876 1 GTACATG 6270 0.0 39.22043 1 ATCAACG 2155 0.0 39.218723 3 TCAACGC 2230 0.0 38.16661 4 CAACGCA 2235 0.0 38.081226 5 TACATGG 6510 0.0 37.577328 2 ACATGGG 6490 0.0 37.050076 3 AACGCAG 2415 0.0 35.481045 6 TATCAAC 2485 0.0 34.251118 2 TAAGGTG 1150 0.0 33.12337 5 CATGGGG 3920 0.0 30.973944 4 ACGCAGA 2950 0.0 28.84043 7 AGGTAAG 1135 0.0 28.318026 2 GTATAGG 655 0.0 28.1806 1 GGTAAGG 1285 0.0 27.790768 3 GTAAGGT 1230 0.0 27.58177 4 CATGGGA 3220 0.0 27.171516 4 CGCAGAG 3160 0.0 26.92303 8 CCGTTGC 225 6.561095E-9 26.44189 9 >>END_MODULE