FastQCFastQC Report
Thu 26 May 2016
SRR1364360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1714260
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.13591870544724838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21920.12786858469543708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22500.068.565491
GTATCAA40700.054.4423561
ATCAACG48850.044.985663
TCAACGC50250.043.8508454
CAACGCA51000.043.0892075
TATCAAC52550.041.931592
AACGCAG53350.041.265466
GTACATG63100.037.9476661
TACATGG64850.036.4579962
ACGCAGA60750.036.1388367
ACATGGG63350.036.0051353
CGCAGAG63750.034.5315138
TAACGTG953.2694868E-431.3443075
CATGGGA33150.030.360964
TAAGGTG11150.029.9106225
GTGGTAT11800.029.2775251
CATGGGG37700.028.9082664
TGGTATC11950.028.4065992
GCAGAGT77800.028.1424759
GAGTACT45450.026.77031512-13
Produced by FastQC (version 0.11.5)