FastQCFastQC Report
Thu 26 May 2016
SRR1364359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000836
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.050.0609661
GTATCAA28600.046.210121
GTACATG96450.041.0458831
CAACGCA32000.039.612095
ATCAACG32150.039.2431533
TCAACGC32450.039.0637474
TACATGG100800.038.553312
AACGCAG34000.037.2801066
ACATGGG100800.037.07733
TATCAAC38650.032.9513552
CATGGGA75850.031.8551374
ACGCAGA44700.028.3498547
CATGGGG35800.028.260184
ATGGGAT31250.026.8514215
CGCAGAG47050.026.8074178
GAGTACA69250.025.1022831
AGTACAT70900.023.4189262
ATGGGAG34500.022.4244825
TAGACCG1653.2123658E-421.640415
ATGGGGA22700.021.4974125
Produced by FastQC (version 0.11.5)