##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364359_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2000836 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.639663620606587 31.0 31.0 34.0 27.0 34.0 2 30.716806374935278 31.0 31.0 34.0 27.0 34.0 3 30.814487044415433 31.0 31.0 34.0 27.0 34.0 4 34.26401364229752 37.0 35.0 37.0 30.0 37.0 5 34.335252864302724 37.0 35.0 37.0 30.0 37.0 6 34.478348550305974 37.0 35.0 37.0 31.0 37.0 7 34.45280122908624 37.0 35.0 37.0 31.0 37.0 8 34.41471514906769 37.0 35.0 37.0 30.0 37.0 9 35.96545893816385 39.0 37.0 39.0 30.0 39.0 10-11 35.870173517469695 39.0 37.0 39.0 30.0 39.0 12-13 35.86852620604587 39.0 37.0 39.0 30.0 39.0 14-15 37.12698916852756 40.0 38.0 41.0 31.0 41.0 16-17 37.12622298879069 40.0 38.0 41.0 31.0 41.0 18-19 37.117925457158904 40.0 37.5 41.0 31.0 41.0 20-21 37.12986546623512 40.0 38.0 41.0 30.5 41.0 22-23 37.08662429104634 40.0 37.0 41.0 30.0 41.0 24-25 37.081454202143505 40.0 37.0 41.0 30.0 41.0 26-27 37.017942749930526 40.0 37.0 41.0 30.0 41.0 28-29 37.00970669260249 40.0 37.0 41.0 30.0 41.0 30-31 36.917943549596274 40.0 37.0 41.0 30.0 41.0 32-33 36.87534535564134 40.0 37.0 41.0 30.0 41.0 34-35 36.85958969150895 40.0 37.0 41.0 30.0 41.0 36-37 36.806573352338724 40.0 37.0 41.0 30.0 41.0 38-39 36.7194397741744 40.0 37.0 41.0 30.0 41.0 40-41 36.66230765540004 40.0 37.0 41.0 30.0 41.0 42-43 36.540744218916494 40.0 36.5 41.0 30.0 41.0 44-45 36.419436675469655 40.0 36.0 41.0 29.0 41.0 46-47 36.30906780965556 40.0 36.0 41.0 29.0 41.0 48-49 36.16981976533809 40.0 36.0 41.0 29.0 41.0 50-51 36.057213084930495 40.0 35.0 41.0 28.0 41.0 52-53 35.90318421899646 39.5 35.0 41.0 28.0 41.0 54-55 35.72253872881136 39.0 35.0 41.0 28.0 41.0 56-57 35.53654372472306 39.0 35.0 41.0 28.0 41.0 58-59 35.31813901789052 39.0 35.0 41.0 27.5 41.0 60-61 35.071265710932835 38.0 34.0 40.0 27.0 41.0 62-63 34.806862231587196 38.0 34.0 40.0 26.0 41.0 64-65 34.47896629208991 37.0 34.0 40.0 26.0 41.0 66-67 34.16528216205626 37.0 34.0 40.0 26.0 41.0 68-69 33.83911100160133 36.0 33.0 39.0 26.0 41.0 70-71 33.49429238578274 36.0 33.0 39.0 26.0 41.0 72-73 33.128311865640164 35.5 33.0 38.5 25.0 40.0 74-75 32.769897432873066 35.0 33.0 37.5 24.5 40.0 76-77 32.5309035823026 35.0 32.5 37.0 24.5 39.0 78-79 32.371553190766264 35.0 33.0 37.0 25.0 39.0 80-81 32.097548224842015 35.0 33.0 36.0 24.5 38.5 82-83 31.807282305996097 35.0 32.5 36.0 24.0 37.0 84-85 31.554437745022582 35.0 32.0 35.5 23.5 37.0 86-87 31.34243211337661 35.0 32.0 35.0 23.0 36.5 88-89 31.087377476214943 35.0 32.0 35.0 21.0 36.0 90-91 30.965005127856557 35.0 32.0 35.0 20.5 36.0 92-93 30.819601156716494 35.0 32.0 35.0 20.0 36.0 94-95 30.717472846350226 35.0 32.0 35.0 20.0 35.0 96-97 30.616706466696918 35.0 32.0 35.0 19.0 35.0 98-99 30.48742575603398 35.0 32.0 35.0 18.5 35.0 100-101 30.401955482608273 35.0 31.0 35.0 18.0 35.0 102-103 29.393879858219265 33.5 29.5 34.5 16.0 35.0 104-105 30.107309394673024 34.0 31.0 35.0 17.0 35.0 106-107 30.46361495894716 35.0 32.0 35.0 18.0 35.0 108-109 30.51194300782273 35.0 32.0 35.0 18.0 35.0 110-111 30.456167072163836 35.0 32.0 35.0 16.5 35.0 112-113 30.371302045744876 35.0 32.0 35.0 12.5 35.0 114-115 30.28086959650866 35.0 32.0 35.0 7.5 35.0 116-117 30.15362553452657 35.0 32.0 35.0 2.0 35.0 118-119 29.98850805363358 35.0 32.0 35.0 2.0 35.0 120-121 29.805192929355528 35.0 31.5 35.0 2.0 35.0 122-123 29.62413361215012 35.0 31.0 35.0 2.0 35.0 124-125 28.781829945082954 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22814967197773584 1101 2 0.27089211494256205 1101 3 0.2642435144103956 1101 4 0.41098678491332663 1101 5 0.2921782675947995 1101 6 0.38257064027186516 1101 7 0.4058190043022094 1101 8 0.29547425836414476 1101 9 0.4639747066779947 1101 10-11 0.5835588534806391 1101 12-13 0.6270282609690625 1101 14-15 0.6437151605615341 1101 16-17 0.646513414189748 1101 18-19 0.5343949587511077 1101 20-21 0.6062591250006975 1101 22-23 0.665793675555534 1101 24-25 0.6805130685948981 1101 26-27 0.8947373679917519 1101 28-29 0.7625214877986508 1101 30-31 0.8225814975395025 1101 32-33 0.6819650106889981 1101 34-35 0.7081165040392818 1101 36-37 0.6769460852611431 1101 38-39 0.6877851035986211 1101 40-41 0.6602978015778618 1101 42-43 0.8113278548101022 1101 44-45 0.7865761175913875 1101 46-47 0.7507628711869643 1101 48-49 0.6820590118202645 1101 50-51 0.6833772877804876 1101 52-53 0.5583964700981596 1101 54-55 0.7198486484355442 1101 56-57 0.5613068092858811 1101 58-59 0.6583466760764338 1101 60-61 0.5527996850306707 1101 62-63 0.6421037306245339 1101 64-65 0.6385470736408365 1101 66-67 0.5781325571529763 1101 68-69 0.5662618292188597 1101 70-71 0.617223190936123 1101 72-73 0.6180985387903277 1101 74-75 0.5272980112354446 1101 76-77 0.5945162476775465 1101 78-79 0.6144470802183477 1101 80-81 0.5298574541751435 1101 82-83 0.5222579815665327 1101 84-85 0.5649937851003024 1101 86-87 0.46545753602476125 1101 88-89 0.5299090001990159 1101 90-91 0.5261301249523811 1101 92-93 0.3850142580753939 1101 94-95 0.3803417846948207 1101 96-97 0.4348927834626686 1101 98-99 0.5365760998179887 1101 100-101 0.4710750562363941 1101 102-103 0.24252766992689345 1101 104-105 0.40704710039243963 1101 106-107 0.44640749894956144 1101 108-109 0.5396990134356123 1101 110-111 0.4560350371861084 1101 112-113 0.4372531643537556 1101 114-115 0.4675551254361707 1101 116-117 0.3751618920999391 1101 118-119 0.47698152584258224 1101 120-121 0.41994909554264837 1101 122-123 0.34077034044324606 1101 124-125 0.3060734069078386 1102 1 0.24248237633041114 1102 2 0.26886025979054295 1102 3 0.19091437690597957 1102 4 0.30845333175990675 1102 5 0.33955495528115875 1102 6 0.33381013214745536 1102 7 0.36627565914157856 1102 8 0.36176571761620124 1102 9 0.4106419339433103 1102 10-11 0.4733726382785477 1102 12-13 0.4429504986930439 1102 14-15 0.45887244096049784 1102 16-17 0.5809393755445882 1102 18-19 0.5794898839672982 1102 20-21 0.5727504628658266 1102 22-23 0.5827712992841683 1102 24-25 0.6130725789560714 1102 26-27 0.5979598496351954 1102 28-29 0.5989938782956017 1102 30-31 0.6518960983745643 1102 32-33 0.7005464347095582 1102 34-35 0.5068799942807942 1102 36-37 0.39751297332097124 1102 38-39 0.535461337566197 1102 40-41 0.48760080203646083 1102 42-43 0.3751983073915497 1102 44-45 0.46054261324002965 1102 46-47 0.45369918769245743 1102 48-49 0.4315863314969519 1102 50-51 0.4064025623153711 1102 52-53 0.5601403295056926 1102 54-55 0.6429954161926332 1102 56-57 0.5569610674715548 1102 58-59 0.4501897059357489 1102 60-61 0.39157958459296793 1102 62-63 0.44091971793736917 1102 64-65 0.43115582849888057 1102 66-67 0.6027993897158908 1102 68-69 0.5080708629432422 1102 70-71 0.45195467461673644 1102 72-73 0.4742287499513864 1102 74-75 0.44969369884610444 1102 76-77 0.5542701858141541 1102 78-79 0.5948382725679693 1102 80-81 0.5957997990451176 1102 82-83 0.6277610328333125 1102 84-85 0.5954243709215667 1102 86-87 0.5745683584643118 1102 88-89 0.5956692292445105 1102 90-91 0.4554486940700002 1102 92-93 0.5775097987769229 1102 94-95 0.4512577103693616 1102 96-97 0.47493576610414934 1102 98-99 0.4748111400127968 1102 100-101 0.389681034005271 1102 102-103 0.15793203496684427 1102 104-105 0.39092701543812325 1102 106-107 0.42498538694691135 1102 108-109 0.41657041001951356 1102 110-111 0.45106570811284996 1102 112-113 0.42008095305546433 1102 114-115 0.43827014499590433 1102 116-117 0.2947340167573991 1102 118-119 0.24375823755121928 1102 120-121 0.09895430615747358 1102 122-123 0.07644250786603735 1102 124-125 6.050544926985424E-4 1103 1 0.2118571892888994 1103 2 0.2478248156101195 1103 3 0.1381398401713838 1103 4 0.30083398894984015 1103 5 0.1556831131228833 1103 6 0.2152708915131356 1103 7 0.2616359502439849 1103 8 0.2092977940305829 1103 9 0.3281232672418355 1103 10-11 0.4175662254299013 1103 12-13 0.28488837486195706 1103 14-15 0.4216367925034845 1103 16-17 0.2493145703789139 1103 18-19 0.41672579677447175 1103 20-21 0.35474556203403296 1103 22-23 0.44156975948850175 1103 24-25 0.4571192535025759 1103 26-27 0.5305508326290038 1103 28-29 0.3872045107456614 1103 30-31 0.5240505202864654 1103 32-33 0.5328839389377862 1103 34-35 0.5575370311114867 1103 36-37 0.4763282043404402 1103 38-39 0.3415636298456164 1103 40-41 0.4234220889877989 1103 42-43 0.44718030690756905 1103 44-45 0.3580212854135567 1103 46-47 0.3522332779043751 1103 48-49 0.3396911644017635 1103 50-51 0.34522042361898997 1103 52-53 0.35534291370479565 1103 54-55 0.3796118904729653 1103 56-57 0.25403797145087026 1103 58-59 0.17533024384648144 1103 60-61 0.16727114390317865 1103 62-63 0.18647871775529978 1103 64-65 0.22334058018650893 1103 66-67 0.1646838186870454 1103 68-69 0.17425349344107133 1103 70-71 0.3008058583602633 1103 72-73 0.3660102905334881 1103 74-75 0.25211315272730417 1103 76-77 0.23888000551963273 1103 78-79 0.27306095167539723 1103 80-81 0.26481141690447885 1103 82-83 0.3195879425126833 1103 84-85 0.21940950034354856 1103 86-87 0.29051028161397596 1103 88-89 0.346143434891534 1103 90-91 0.22339435292809284 1103 92-93 0.1659839442842852 1103 94-95 0.18148109617289876 1103 96-97 0.17013220416189512 1103 98-99 0.16922730449675427 1103 100-101 0.16725151719441556 1103 102-103 -0.001286235330539398 1103 104-105 0.14988069324836317 1103 106-107 0.14599314044743394 1103 108-109 0.19579996586607606 1103 110-111 0.12073258394658737 1103 112-113 0.17262771907813956 1103 114-115 0.117735159738384 1103 116-117 0.06651071532562014 1103 118-119 -0.06193409167884312 1103 120-121 -0.15696815641412343 1103 122-123 -0.09008818368391402 1103 124-125 -0.17633745386214628 1104 1 0.4003469262505668 1104 2 0.32911524008438064 1104 3 0.37357783090119767 1104 4 0.4577041067997669 1104 5 0.40835550310811897 1104 6 0.46910595497818974 1104 7 0.5445726007309091 1104 8 0.5251329291473965 1104 9 0.6034931264031016 1104 10-11 0.5196340456468249 1104 12-13 0.5096564937461991 1104 14-15 0.6247408520488875 1104 16-17 0.6169356900893135 1104 18-19 0.6499036371883733 1104 20-21 0.7040164623727918 1104 22-23 0.7199162865353799 1104 24-25 0.7827928418795906 1104 26-27 0.6987377860490085 1104 28-29 0.7175874269872367 1104 30-31 0.8120710027911144 1104 32-33 0.6748434445062372 1104 34-35 0.6495065259676807 1104 36-37 0.6973000173353725 1104 38-39 0.5707148244127396 1104 40-41 0.647950346622558 1104 42-43 0.5547968348807473 1104 44-45 0.5233310334844745 1104 46-47 0.5398336616393422 1104 48-49 0.6057340304419156 1104 50-51 0.45061294991039347 1104 52-53 0.4032073632122035 1104 54-55 0.5043359209337837 1104 56-57 0.4569632243595194 1104 58-59 0.523336157160557 1104 60-61 0.39385250409667094 1104 62-63 0.5192849910925474 1104 64-65 0.54802002733188 1104 66-67 0.44239349502013425 1104 68-69 0.44760855038216363 1104 70-71 0.3594360381448496 1104 72-73 0.3409482057922659 1104 74-75 0.38207819721324654 1104 76-77 0.4196413043615763 1104 78-79 0.5536357838128652 1104 80-81 0.41443448670459304 1104 82-83 0.34159541387591474 1104 84-85 0.3780637039079089 1104 86-87 0.38475950729624486 1104 88-89 0.47580046289069955 1104 90-91 0.31057436893032175 1104 92-93 0.35920608614034677 1104 94-95 0.34316312439529995 1104 96-97 0.2709303573970274 1104 98-99 0.19937219919610527 1104 100-101 0.19921094422565488 1104 102-103 0.004866617232416104 1104 104-105 0.22509056242796177 1104 106-107 0.28708136710625354 1104 108-109 0.36300816274100356 1104 110-111 0.3526123548804385 1104 112-113 0.4980867825251245 1104 114-115 0.4401299809301342 1104 116-117 0.42609306734726005 1104 118-119 0.44363026972702 1104 120-121 0.44972685035137516 1104 122-123 0.3844851252619996 1104 124-125 0.26627589012642616 1105 1 -0.09192096892425639 1105 2 -0.15818915238372355 1105 3 -0.17717615896532024 1105 4 -0.10817895601424965 1105 5 -0.0072942245529432626 1105 6 0.10396519339592203 1105 7 0.13083232927955635 1105 8 0.08954386987623053 1105 9 0.119916491671475 1105 10-11 0.18883864967713038 1105 12-13 0.21002516495470047 1105 14-15 0.30859083623485617 1105 16-17 0.31902447620896623 1105 18-19 0.27898085593501776 1105 20-21 0.27688317193251066 1105 22-23 0.2888703245732387 1105 24-25 0.24043117718463947 1105 26-27 0.2867583105416003 1105 28-29 0.2183142683847592 1105 30-31 0.16316514026362938 1105 32-33 0.24305965049623524 1105 34-35 0.17403708455940148 1105 36-37 0.12515332559404868 1105 38-39 0.14087726101595877 1105 40-41 0.16979824893973472 1105 42-43 0.07184859448908298 1105 44-45 0.09048218815907205 1105 46-47 0.2039352181654479 1105 48-49 0.10163743481408716 1105 50-51 0.13249826419298927 1105 52-53 0.07943942032441242 1105 54-55 0.0891375391742173 1105 56-57 0.10119795631474915 1105 58-59 0.13968495268944991 1105 60-61 0.15422929275990072 1105 62-63 0.13379741042325577 1105 64-65 0.1109120185126855 1105 66-67 0.0073831377646200735 1105 68-69 0.10191900010499211 1105 70-71 0.09222316914548401 1105 72-73 0.011327663464619775 1105 74-75 -0.0824527343313548 1105 76-77 0.00850128535139305 1105 78-79 0.10209543635413354 1105 80-81 0.04782988509118269 1105 82-83 0.16662446878000026 1105 84-85 0.11787542312898225 1105 86-87 0.0930480619767593 1105 88-89 0.1362748964502849 1105 90-91 0.19978236287306572 1105 92-93 0.13668625400664425 1105 94-95 0.15006896150364923 1105 96-97 0.08541349263474274 1105 98-99 0.03743830103016066 1105 100-101 -0.01074620994805997 1105 102-103 -0.0797286268058599 1105 104-105 0.004588113648559045 1105 106-107 0.03702578879385854 1105 108-109 0.08359114017173042 1105 110-111 0.07329445651780375 1105 112-113 0.03944043269199682 1105 114-115 -0.06848872038545295 1105 116-117 -0.06294451421794633 1105 118-119 -0.043751942796109944 1105 120-121 -0.11471523923481897 1105 122-123 -0.1838504152300544 1105 124-125 -0.1810161282236855 1106 1 0.4169468441984847 1106 2 0.46694103352585614 1106 3 0.32218869780967907 1106 4 0.5164173651574657 1106 5 0.38369527036672935 1106 6 0.3949665793120616 1106 7 0.4621085202243549 1106 8 0.4462192292713425 1106 9 0.6137946786635453 1106 10-11 0.6387642284406283 1106 12-13 0.4587831708755985 1106 14-15 0.5835229755481279 1106 16-17 0.5535715112505031 1106 18-19 0.5845476874355029 1106 20-21 0.5057116627600351 1106 22-23 0.6926092228929122 1106 24-25 0.5913396083111664 1106 26-27 0.7442186807206213 1106 28-29 0.6657568840759467 1106 30-31 0.7023494589095378 1106 32-33 0.722028168058003 1106 34-35 0.7527827484460374 1106 36-37 0.7213266799211411 1106 38-39 0.6978966392398505 1106 40-41 0.7216632902301967 1106 42-43 0.6406267330970934 1106 44-45 0.7605246677188617 1106 46-47 0.7426523026571346 1106 48-49 0.6535176473759066 1106 50-51 0.5398501802835582 1106 52-53 0.6788518410852689 1106 54-55 0.6969802663947604 1106 56-57 0.6096868976880359 1106 58-59 0.5763873333489471 1106 60-61 0.4952999744830322 1106 62-63 0.5491642681659386 1106 64-65 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-1.5889879141925825 2216 82-83 -1.593801518295134 2216 84-85 -1.6109447796235408 2216 86-87 -1.6981154355914754 2216 88-89 -1.6801017286779363 2216 90-91 -1.708411853059605 2216 92-93 -1.773459051826137 2216 94-95 -1.5733742829792021 2216 96-97 -1.6342687210582625 2216 98-99 -1.7295039944463078 2216 100-101 -1.5201772400954496 2216 102-103 -1.5998940551478995 2216 104-105 -1.6873787951519468 2216 106-107 -1.606230192328976 2216 108-109 -1.6140462998213287 2216 110-111 -1.4701402424401842 2216 112-113 -1.5274218109794866 2216 114-115 -1.5900745736594075 2216 116-117 -1.7166006884181648 2216 118-119 -1.6966403599828084 2216 120-121 -1.5638737273427807 2216 122-123 -1.539353982141069 2216 124-125 -1.4329730151253557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39436.0 3 6997.0 4 4714.0 5 3799.0 6 4353.0 7 4740.0 8 5256.0 9 5727.0 10 6273.0 11 6907.0 12 7321.0 13 7357.0 14 7429.0 15 7050.0 16 6635.0 17 6102.0 18 5677.0 19 5693.0 20 5658.0 21 6143.0 22 7227.0 23 9363.0 24 13327.0 25 18347.0 26 21666.0 27 22753.0 28 27272.0 29 32414.0 30 38227.0 31 47753.0 32 61125.0 33 80447.0 34 111330.0 35 168315.0 36 328039.0 37 609027.0 38 250433.0 39 504.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34231513472702 18.960934414016208 12.331034965524482 24.365715485732288 2 15.443499592885773 20.430286499452432 39.69391208032121 24.432301827340584 3 18.412317928259373 25.742019031473053 29.298162576700914 26.54750046356666 4 12.043666105537584 16.35654621717695 37.86876576925999 33.731021908025475 5 13.092007782459072 39.12495325925467 34.770765388453086 13.012273569833175 6 31.956562711061142 36.950041218631725 17.963074704958085 13.130321365349054 7 28.592227550686356 32.4201251008979 21.739767141397 17.247880207018746 8 26.311315025976654 35.61198199751603 20.318017227807392 17.758685748699918 9 27.051142622383846 15.479629514862786 19.607004272214215 37.86222359053916 10-11 24.583317610726922 26.920692341120667 28.33687643830612 20.15911360984629 12-13 25.089250862520935 24.30312356777543 28.083071898985466 22.52455367071817 14-15 22.342349996813827 25.767324351238795 25.2737298099666 26.61659584198078 16-17 22.656133056548683 28.985670221935006 26.03272586149722 22.325470860019088 18-19 22.69094354927584 28.092717360040204 27.149635389451447 22.066703701232512 20-21 23.51315238059301 27.44066166359004 26.87324870035543 22.17293725546153 22-23 23.377084223065815 27.33548060297811 26.592016185238617 22.695418988717464 24-25 22.931689553766528 27.348818193994912 26.942538019108014 22.77695423313055 26-27 22.765895238737826 27.669348047190294 26.795687800343355 22.769068913728518 28-29 22.781311583725376 27.53270762727324 26.898746672011107 22.787234116990273 30-31 22.86466543451381 27.370811371658014 26.918774870878842 22.84574832294933 32-33 22.799200633935175 27.41642178979731 26.736555803812955 23.047821772454558 34-35 23.172762580960516 27.29799417339182 26.628518992953392 22.900724252694268 36-37 23.153281658189364 27.054681725061513 26.703652928555254 23.08838368819387 38-39 22.814955459834387 27.32879705942695 26.796394204518165 23.059853276220498 40-41 23.0781437266409 26.99625054852346 26.893592946113255 23.03201277872239 42-43 22.794830036487422 27.027156027771525 26.91912567131633 23.258888264424726 44-45 22.72155460572087 27.191180998152753 26.89805059535709 23.189213800769288 46-47 23.03526353628948 27.307206068268012 26.602924929573135 23.054605465869365 48-49 22.866691722859844 27.21282503913364 26.723479585533248 23.197003652473267 50-51 22.77570475541224 27.149976309902463 26.91175088812876 23.16256804655654 52-53 23.010075384205553 27.08798455830793 26.73757769950975 23.164362357976767 54-55 22.701179514104865 27.158812693939844 26.850883017460923 23.289124774494365 56-57 22.68666801777161 27.329562734293216 26.87385219594953 23.109917051985637 58-59 22.81621246504868 26.912036249165027 26.78478832537784 23.48696296040845 60-61 22.7808079439946 26.936905958445767 27.085945820861284 23.19634027669835 62-63 22.631530019986833 27.095706403933995 27.069492112472055 23.203271463607116 64-65 22.63151436788862 26.93896961638066 26.962910058159277 23.46660595757144 66-67 22.511649247565817 27.183193695636636 26.703129240548527 23.60202781624902 68-69 22.512832559464606 26.880800891656715 27.154295596195468 23.45207095268322 70-71 22.447585249174516 26.760067802997455 27.179075933009678 23.61327101481835 72-73 22.551444401711034 26.844327689774445 26.81378954620868 23.790438362305835 74-75 22.524575317324974 27.019904157761843 26.871512624026135 23.58400790088705 76-77 22.5614971992488 26.768684065518713 27.031853468795646 23.63796526643684 78-79 22.614427105395013 26.70992474194471 27.12996040299233 23.54568774966794 80-81 22.55950044094763 26.996415691787977 26.969776407440104 23.47430745982429 82-83 22.560497756417625 26.953550997641962 26.976816482058837 23.509134763881573 84-85 22.622360243169876 26.71512504726179 27.0339437736512 23.628570935917136 86-87 22.55351976181563 26.63810480092243 27.16919156958481 23.639183867677126 88-89 22.589487068758697 26.645494458039444 27.12049465796587 23.644523815235992 90-91 22.57584795498465 26.756990916512834 27.18741261432452 23.479748514178 92-93 22.548668771469313 26.421878818744904 27.19281235053011 23.836640059255675 94-95 22.432983559199627 26.3404912498157 27.296626624650454 23.929898566334217 96-97 22.545204286113762 26.361513839107552 27.261163909940535 23.832117964838154 98-99 22.5213349493334 26.207014606474825 27.118713530668597 24.15293691352317 100-101 22.24552123012764 26.24646985044236 27.408429775982164 24.099579143447837 102-103 22.374167113779823 26.0370047258632 27.398661084561986 24.19016707579499 104-105 22.29561453707614 26.191077136067054 27.321616441300172 24.191691885556637 106-107 22.248946302342816 26.23619071896356 27.34598145392158 24.168881524772047 108-109 22.326975263027286 26.239678766967355 27.128227568053724 24.305118401951628 110-111 22.133660269797552 26.381518560233385 27.314771892093976 24.170049277875087 112-113 22.088513090536054 26.37657110798177 27.54504186497499 23.989873936507184 114-115 22.090661663255894 26.43651676843173 27.337570538520573 24.135251029791803 116-117 21.95227641047235 26.390267856361916 27.426807127675655 24.230648605490074 118-119 21.869083456190356 26.550592301271003 27.16903167998656 24.411292562552077 120-121 21.872082096192106 26.624453908366576 27.375261174259464 24.12820282118186 122-123 21.971005017929745 26.68172120563266 27.32266370374147 24.024610072696127 124-125 21.84808533523127 26.704195588893864 27.246437368050668 24.201281707824197 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 709.0 1 591.0 2 396.0 3 278.5 4 200.5 5 155.5 6 151.5 7 147.5 8 152.5 9 155.0 10 137.0 11 126.0 12 126.5 13 122.0 14 139.0 15 188.0 16 211.5 17 238.0 18 345.0 19 489.5 20 601.5 21 818.5 22 1224.5 23 1790.0 24 2635.5 25 3852.0 26 5855.5 27 8617.0 28 11375.5 29 14827.5 30 19261.0 31 23667.0 32 28848.0 33 33671.5 34 38085.5 35 42543.5 36 48292.5 37 54664.5 38 58470.0 39 61255.0 40 62886.0 41 65151.0 42 67874.5 43 71170.5 44 76519.5 45 80089.0 46 79645.0 47 78896.0 48 79314.0 49 78691.5 50 77644.5 51 75243.0 52 73460.5 53 72209.0 54 70417.5 55 65865.0 56 57735.5 57 51992.5 58 49158.0 59 44455.0 60 36808.0 61 29181.0 62 22895.0 63 17520.0 64 13359.0 65 10289.0 66 7483.5 67 5917.0 68 4452.0 69 2694.5 70 1815.5 71 1320.5 72 1005.0 73 774.0 74 572.5 75 441.0 76 295.0 77 168.5 78 106.0 79 73.5 80 45.5 81 23.5 82 13.0 83 12.5 84 11.0 85 12.0 86 11.5 87 8.5 88 9.0 89 6.0 90 4.0 91 5.5 92 4.5 93 3.5 94 2.5 95 2.0 96 2.5 97 2.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043281908162388125 2 0.008446469375800915 3 0.0022490598929647406 4 0.005397743743115378 5 0.021990807842321908 6 0.026338990302053743 7 0.0025489345453600393 8 4.9979108732549796E-5 9 0.0 10-11 7.496866309882469E-5 12-13 7.49686630988247E-4 14-15 1.749268805639243E-4 16-17 1.2494777183137448E-4 18-19 1.2494777183137448E-4 20-21 2.4989554366274898E-5 22-23 2.4989554366274898E-5 24-25 0.0 26-27 4.9979108732549796E-5 28-29 1.4993732619764937E-4 30-31 9.995821746509959E-5 32-33 2.4989554366274898E-5 34-35 7.49686630988247E-4 36-37 1.4993732619764937E-4 38-39 7.496866309882469E-5 40-41 4.997910873254979E-4 42-43 4.7480153295922304E-4 44-45 9.995821746509959E-4 46-47 7.496866309882469E-5 48-49 0.0 50-51 0.0 52-53 9.496030659184461E-4 54-55 0.002723861425923964 56-57 2.2490598929647408E-4 58-59 8.746344028196214E-4 60-61 0.001449394153243944 62-63 9.496030659184461E-4 64-65 0.0018742165774706173 66-67 0.003148683850150637 68-69 0.0032986211763482865 70-71 0.001974174794935717 72-73 0.0029737569695867127 74-75 0.00239899721916239 76-77 0.001674300142540418 78-79 0.002673882317191414 80-81 0.0015743419250753186 82-83 8.996239571858963E-4 84-85 6.247388591568725E-4 86-87 0.0016493105881741433 88-89 0.00499791087325498 90-91 3.748433154941235E-4 92-93 7.49686630988247E-4 94-95 0.0010495612833835456 96-97 1.2494777183137448E-4 98-99 5.497701960580477E-4 100-101 0.0017742583600055177 102-103 0.0018742165774706173 104-105 0.0010495612833835456 106-107 0.007172002103120896 108-109 0.0022740494473310158 110-111 0.001974174794935717 112-113 0.002923777860854163 114-115 0.003373589839447111 116-117 0.002174091229865916 118-119 0.0441065634564752 120-121 0.012794651835532748 122-123 0.0055226915149467525 124-125 0.0033985793938133856 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2000836.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.449751332437856 #Duplication Level Percentage of deduplicated Percentage of total 1 82.13790102696103 52.11629395129069 2 10.124199000154038 12.847558179997792 3 3.4216394434690733 6.513065155121213 4 1.4841132269471649 3.7666646079591803 5 0.7817498137904315 2.4800915644591237 6 0.46326367029132676 1.7636378808802298 7 0.3078076342937623 1.367122249791563 8 0.2241111662514515 1.137583821558179 9 0.15193108465374366 0.8675990586852841 >10 0.792191143105399 9.358938972050407 >50 0.0702053348352396 3.0473822231188605 >100 0.04032735312743574 4.470130928349258 >500 3.7340141323138963E-4 0.1429825888388602 >1k 1.8670070661569481E-4 0.12094881789931752 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.9979108732549796E-5 0.0 0.0 0.0 0.0 7 4.9979108732549796E-5 0.0 0.0 0.0 0.0 8 4.9979108732549796E-5 0.0 0.0 0.0 0.0 9 4.9979108732549796E-5 0.0 0.0 4.9979108732549796E-5 0.0 10-11 4.9979108732549796E-5 0.0 0.0 4.9979108732549796E-5 0.0 12-13 4.9979108732549796E-5 0.0 0.0 4.9979108732549796E-5 0.0 14-15 1.499373261976494E-4 0.0 0.0 4.9979108732549796E-5 0.0 16-17 2.2490598929647408E-4 0.0 0.0 4.9979108732549796E-5 0.0 18-19 3.9983286986039837E-4 0.0 0.0 4.9979108732549796E-5 0.0 20-21 4.997910873254979E-4 0.0 0.0 1.499373261976494E-4 0.0 22-23 6.497284135231473E-4 0.0 0.0 2.2490598929647408E-4 0.0 24-25 9.995821746509959E-4 0.0 0.0 2.9987465239529875E-4 0.0 26-27 9.995821746509959E-4 0.0 0.0 9.496030659184461E-4 0.0 28-29 0.0013494359357788446 0.0 0.0 0.003123694295784362 0.0 30-31 0.001499373261976494 0.0 0.0 0.014244045988776693 0.0 32-33 0.001499373261976494 0.0 0.0 0.03750932110377862 0.0 34-35 0.001699289696906693 0.0 0.0 0.07311943607572036 0.0 36-37 0.0018992061318368922 0.0 0.0 0.13284447101111735 0.0 38-39 0.0020991225667670913 0.0 0.0 0.22460611464407876 0.0 40-41 0.0021241121211333664 0.0 0.0 0.3243644156742481 0.0 42-43 0.00239899721916239 0.0 0.0 0.42487240333540577 0.0 44-45 0.002648892762825139 0.0 0.0 0.5475961048281819 0.0 46-47 0.003123694295784362 0.0 0.0 0.6933601754466633 0.0 48-49 0.0032486420676157367 0.0 0.0 0.8493449738009512 0.0 50-51 0.0032486420676157367 0.0 0.0 1.0257462380724858 0.0 52-53 0.0032486420676157367 0.0 0.0 1.1892778818453886 0.0 54-55 0.0032986211763482865 0.0 0.0 1.3649044699315687 0.0 56-57 0.0036984540462086848 0.0 0.0 1.5749666639344753 0.0 58-59 0.0036984540462086848 0.0 0.0 1.810843067597744 0.0 60-61 0.0036984540462086848 0.0 0.0 2.066536187873469 0.0 62-63 0.0037734227093075097 0.0 0.0 2.3221543394860946 0.0 64-65 0.003898370481138884 0.0 0.0 2.5767229298153373 0.0 66-67 0.004148266024801633 0.0 0.0 2.8665018022466606 0.0 68-69 0.004148266024801633 0.0 0.0 3.192865382270211 0.0 70-71 0.0041982451335341835 0.0 0.0 3.5504159261428723 0.0 72-73 0.004348182459731832 0.0 0.0 3.9139889526178058 0.0 74-75 0.004448140677196932 0.0 0.0 4.27768692686457 0.0 76-77 0.004448140677196932 0.0 0.0 4.673946290450592 0.0 78-79 0.004448140677196932 0.0 0.0 5.110563784338147 0.0 80-81 0.005097869090720079 0.0 0.0 5.578743085390307 0.0 82-83 0.005197827308185178 0.0 0.0 6.072311773678603 0.0 84-85 0.0053227750800165525 0.0 0.0 6.548737627671633 0.0 86-87 0.005497701960580477 0.0 0.0 7.04008224562133 0.0 88-89 0.005572670623679303 0.0 0.0 7.567511780075929 0.0 90-91 0.0062223990372024494 0.0 0.0 8.157615116881143 0.0 92-93 0.006347346809033824 0.0 0.0 8.770084104844175 0.0 94-95 0.006347346809033824 0.0 0.0 9.38467720492834 0.0 96-97 0.006347346809033824 0.0 0.0 9.976604779202294 0.0 98-99 0.006572252798330298 0.0 0.0 10.608140797146792 0.0 100-101 0.0068471378963593215 0.0 0.0 11.305774186390089 0.0 102-103 0.00709703344002207 0.0 0.0 12.042866081977733 0.0 104-105 0.008171584277771891 0.0 0.0 12.763664788118568 0.0 106-107 0.008496448484533466 0.0 0.0 13.468320242138786 0.0 108-109 0.008621396256364839 0.0 0.0 14.198465041612607 0.0 110-111 0.00872135447382994 0.0 0.0 14.975790119729954 0.0 112-113 0.008746344028196214 0.0 0.0 15.809591590715081 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1760 0.0 50.060966 1 GTATCAA 2860 0.0 46.21012 1 GTACATG 9645 0.0 41.045883 1 CAACGCA 3200 0.0 39.61209 5 ATCAACG 3215 0.0 39.243153 3 TCAACGC 3245 0.0 39.063747 4 TACATGG 10080 0.0 38.55331 2 AACGCAG 3400 0.0 37.280106 6 ACATGGG 10080 0.0 37.0773 3 TATCAAC 3865 0.0 32.951355 2 CATGGGA 7585 0.0 31.855137 4 ACGCAGA 4470 0.0 28.349854 7 CATGGGG 3580 0.0 28.26018 4 ATGGGAT 3125 0.0 26.851421 5 CGCAGAG 4705 0.0 26.807417 8 GAGTACA 6925 0.0 25.102283 1 AGTACAT 7090 0.0 23.418926 2 ATGGGAG 3450 0.0 22.424482 5 TAGACCG 165 3.2123658E-4 21.64041 5 ATGGGGA 2270 0.0 21.497412 5 >>END_MODULE