FastQCFastQC Report
Thu 26 May 2016
SRR1364359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000836
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.1376924445581747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24880.12434802252658389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29600.068.62141
GTATCAA52650.053.5130461
ATCAACG65950.042.251613
TCAACGC67050.041.4696464
CAACGCA67000.041.4117245
AACGCAG69850.039.7864046
TATCAAC70550.039.496722
GTACATG103350.037.1745871
TACATGG105600.035.2957652
ACATGGG104900.034.5663873
ACGCAGA84900.032.7971047
ACCGTAT750.003912037231.7319038
CGCAGAG88200.031.5700048
CATGGGA77400.030.847194
ATGGGAT33000.028.14645
GCAGAGT101800.027.1186189
CATGGGG40100.026.2809524
TGGTATC16750.024.8825822
GAGTACT47950.024.25802812-13
AGAGTAC98150.023.3685310-11
Produced by FastQC (version 0.11.5)