FastQCFastQC Report
Thu 26 May 2016
SRR1364358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223349
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.049.5478131
GTATCAA25200.043.9397851
CAACGCA27300.039.0201875
ATCAACG27750.038.1738323
TCAACGC28550.037.1041644
GTACATG75650.035.2545051
AACGCAG30700.034.8886576
TACATGG75550.034.1872372
TAAGGTG16550.033.441365
TATCAAC32350.033.1136442
ACATGGG76150.032.980053
AGGTAAG14400.031.8226952
GTAAGGT16900.029.5802174
GGTAAGG16750.029.1345163
CGCAGAG37850.027.978618
ACGCAGA38700.027.2103617
GAGTACA54200.027.0197531
CATGGGA33950.026.2942164
CATGGGG46850.025.7866674
TAAGGTA9000.024.4662824
Produced by FastQC (version 0.11.5)