##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364358_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2223349 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.866371856150337 31.0 31.0 34.0 27.0 34.0 2 30.84605925565442 31.0 31.0 34.0 27.0 34.0 3 30.99556614818456 33.0 31.0 34.0 27.0 34.0 4 34.475976106315294 37.0 35.0 37.0 31.0 37.0 5 34.5035862565886 37.0 35.0 37.0 31.0 37.0 6 34.574385757701556 37.0 35.0 37.0 31.0 37.0 7 34.468044378098085 37.0 35.0 37.0 31.0 37.0 8 34.351562440264665 37.0 35.0 37.0 30.0 37.0 9 35.88622074177288 39.0 37.0 39.0 30.0 39.0 10-11 35.80408833700872 39.0 37.0 39.0 30.0 39.0 12-13 35.846334740969596 39.0 37.0 39.0 30.0 39.0 14-15 37.118131926206814 40.0 37.0 41.0 31.0 41.0 16-17 37.1109888730919 40.0 37.0 41.0 31.0 41.0 18-19 37.094184493752444 40.0 37.0 41.0 31.0 41.0 20-21 37.11404687253328 40.0 37.0 41.0 30.5 41.0 22-23 37.07427736266326 40.0 37.0 41.0 30.0 41.0 24-25 37.05594780666463 40.0 37.0 41.0 30.0 41.0 26-27 36.997298219937576 40.0 37.0 41.0 30.0 41.0 28-29 36.99251556998024 40.0 37.0 41.0 30.0 41.0 30-31 36.885208979786796 40.0 37.0 41.0 30.0 41.0 32-33 36.83867332569021 40.0 37.0 41.0 30.0 41.0 34-35 36.828957127288604 40.0 37.0 41.0 30.0 41.0 36-37 36.75757607105317 40.0 37.0 41.0 30.0 41.0 38-39 36.64865030186444 40.0 37.0 41.0 30.0 41.0 40-41 36.58069043591447 40.0 36.0 41.0 30.0 41.0 42-43 36.430271630769624 40.0 36.0 41.0 29.5 41.0 44-45 36.285096042051876 40.0 36.0 41.0 29.0 41.0 46-47 36.15881897084084 40.0 35.0 41.0 29.0 41.0 48-49 35.990635973029875 39.5 35.0 41.0 28.0 41.0 50-51 35.84894499244159 39.0 35.0 41.0 28.0 41.0 52-53 35.66287029161863 39.0 35.0 41.0 28.0 41.0 54-55 35.468180434110884 39.0 35.0 41.0 28.0 41.0 56-57 35.26267086273904 39.0 34.5 40.5 27.0 41.0 58-59 35.031637858024084 38.0 34.0 40.0 27.0 41.0 60-61 34.77540098293161 38.0 34.0 40.0 26.0 41.0 62-63 34.496863740240514 37.5 34.0 40.0 26.0 41.0 64-65 34.14353077272169 37.0 34.0 40.0 26.0 41.0 66-67 33.830879902345515 36.0 33.0 39.5 26.0 41.0 68-69 33.50223041906601 36.0 33.0 39.0 25.0 41.0 70-71 33.12835794110596 35.0 33.0 39.0 25.0 40.5 72-73 32.78160131405371 35.0 32.5 38.0 24.0 40.0 74-75 32.434282696958505 35.0 32.0 37.0 23.5 39.5 76-77 32.21600972227032 35.0 32.0 37.0 23.5 39.0 78-79 32.06363485894477 35.0 32.0 36.0 24.0 39.0 80-81 31.82819588827485 35.0 32.0 36.0 23.5 38.0 82-83 31.545713021212592 35.0 32.0 35.5 23.0 37.0 84-85 31.312505144266602 35.0 32.0 35.0 22.0 37.0 86-87 31.129159659594603 35.0 32.0 35.0 21.5 36.0 88-89 30.87503986103846 35.0 32.0 35.0 20.0 36.0 90-91 30.75855792320504 35.0 32.0 35.0 20.0 36.0 92-93 30.622609855672682 35.0 32.0 35.0 19.0 35.5 94-95 30.515014062119803 35.0 31.0 35.0 19.0 35.0 96-97 30.420038419519383 35.0 31.0 35.0 18.0 35.0 98-99 30.289198187059252 35.0 31.0 35.0 17.5 35.0 100-101 30.195358668387193 34.0 31.0 35.0 16.0 35.0 102-103 29.161046466389216 33.5 29.5 34.5 10.5 35.0 104-105 29.887836997250545 34.0 31.0 35.0 10.5 35.0 106-107 30.260663755442806 35.0 31.5 35.0 15.0 35.0 108-109 30.321360928940983 35.0 32.0 35.0 12.5 35.0 110-111 30.24840904419414 35.0 32.0 35.0 8.5 35.0 112-113 30.15593728200116 35.0 32.0 35.0 6.0 35.0 114-115 30.054536872079012 35.0 32.0 35.0 2.0 35.0 116-117 29.922610215490238 35.0 31.5 35.0 2.0 35.0 118-119 29.73889366896515 35.0 31.0 35.0 2.0 35.0 120-121 29.560519963352583 35.0 31.0 35.0 2.0 35.0 122-123 29.378690435014924 35.0 31.0 35.0 2.0 35.0 124-125 28.520426842569474 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3802401039834251 1101 2 0.2578284450767967 1101 3 0.3845457415660576 1101 4 0.5374561911857043 1101 5 0.3692464153715349 1101 6 0.3517451729058152 1101 7 0.3176063746813824 1101 8 0.14841463581075942 1101 9 0.500670895701866 1101 10-11 0.47335595690795884 1101 12-13 0.535798932311998 1101 14-15 0.6761173097783484 1101 16-17 0.6254097887182155 1101 18-19 0.4241050410986844 1101 20-21 0.560007307273608 1101 22-23 0.730190412839228 1101 24-25 0.7897552053853829 1101 26-27 0.8613630419038785 1101 28-29 0.741471285926032 1101 30-31 0.7473891748696389 1101 32-33 0.7145492416594195 1101 34-35 0.732202661976487 1101 36-37 0.6668848594362728 1101 38-39 0.7524832213110457 1101 40-41 0.617792154781533 1101 42-43 0.7386845541323837 1101 44-45 0.7695549801480368 1101 46-47 0.7949056004986303 1101 48-49 0.6100426983115739 1101 50-51 0.6562508066110695 1101 52-53 0.6153334494081975 1101 54-55 0.6302331316793826 1101 56-57 0.5545633122688187 1101 58-59 0.6615011847469461 1101 60-61 0.6342095332278177 1101 62-63 0.741303717444282 1101 64-65 0.6591143528008416 1101 66-67 0.6065639319453098 1101 68-69 0.47739397380286874 1101 70-71 0.4852314806566298 1101 72-73 0.4834987111920057 1101 74-75 0.4302885365902682 1101 76-77 0.4297217840933314 1101 78-79 0.5375272059705267 1101 80-81 0.5765881114508318 1101 82-83 0.4365825307797806 1101 84-85 0.4513368833728819 1101 86-87 0.5266451640991932 1101 88-89 0.5836187378618476 1101 90-91 0.5084627410277989 1101 92-93 0.48721276275572123 1101 94-95 0.44493727549953377 1101 96-97 0.3619417903014224 1101 98-99 0.36639333768204807 1101 100-101 0.33246458448964944 1101 102-103 0.08058870635016646 1101 104-105 0.2340184372328622 1101 106-107 0.31428603046271775 1101 108-109 0.43761146477215007 1101 110-111 0.4362203487871419 1101 112-113 0.44931603984255375 1101 114-115 0.4601556586414546 1101 116-117 0.5478937568916891 1101 118-119 0.5524103368861333 1101 120-121 0.4598116057654025 1101 122-123 0.3991855146730039 1101 124-125 0.2775317501457266 1102 1 0.3838580393087412 1102 2 0.3432846511688794 1102 3 0.4357940974249175 1102 4 0.4677091396421531 1102 5 0.44998181019269623 1102 6 0.42526953332207995 1102 7 0.41327916749980886 1102 8 0.4998618099408887 1102 9 0.5014127754409188 1102 10-11 0.5906107599262853 1102 12-13 0.5194672678033072 1102 14-15 0.632232946605356 1102 16-17 0.6827494400503156 1102 18-19 0.7113820006143214 1102 20-21 0.7831314192174474 1102 22-23 0.7364190866606961 1102 24-25 0.7648823009779875 1102 26-27 0.6567069240125392 1102 28-29 0.6059546938475293 1102 30-31 0.6831504543743634 1102 32-33 0.7343215510233634 1102 34-35 0.6383704090962183 1102 36-37 0.5370106316510501 1102 38-39 0.6126239520942036 1102 40-41 0.5919941689552317 1102 42-43 0.46593959359909576 1102 44-45 0.5042984226459879 1102 46-47 0.5175628185165166 1102 48-49 0.49929856589754706 1102 50-51 0.4576703126135584 1102 52-53 0.44060716133267874 1102 54-55 0.4865868390435182 1102 56-57 0.4023511267818378 1102 58-59 0.49093350148661585 1102 60-61 0.3740373951331577 1102 62-63 0.40700088460926054 1102 64-65 0.2913690110174443 1102 66-67 0.3780017819439223 1102 68-69 0.2595973282173816 1102 70-71 0.2404566693387764 1102 72-73 0.2904353921457741 1102 74-75 0.32872593348776746 1102 76-77 0.33551671258693716 1102 78-79 0.4075520752090682 1102 80-81 0.3612114876552468 1102 82-83 0.33577165943263054 1102 84-85 0.32913618487867424 1102 86-87 0.24426300704467252 1102 88-89 0.304432941481668 1102 90-91 0.25230188573714685 1102 92-93 0.41606058457114514 1102 94-95 0.27691278714452494 1102 96-97 0.21561295400875835 1102 98-99 0.29202266615395445 1102 100-101 0.30992234637548677 1102 102-103 0.10379877369946655 1102 104-105 0.2804760554167345 1102 106-107 0.23184499959172555 1102 108-109 0.2511658138095676 1102 110-111 0.2140895033796646 1102 112-113 0.3294357432152992 1102 114-115 0.12547822007037723 1102 116-117 0.12640108385912185 1102 118-119 0.10263389975647641 1102 120-121 0.0014575908350593636 1102 122-123 0.011421288178173228 1102 124-125 -0.018596489930402527 1103 1 0.39408476080456367 1103 2 0.4544220070218543 1103 3 0.4696835314204648 1103 4 0.5811491730100329 1103 5 0.46694669307438375 1103 6 0.46035330212826153 1103 7 0.34689298576982 1103 8 0.4193902865576504 1103 9 0.6389848030171876 1103 10-11 0.7099955029421849 1103 12-13 0.6344540106869161 1103 14-15 0.757359821711006 1103 16-17 0.575486781938757 1103 18-19 0.5736385859927182 1103 20-21 0.6503051728831295 1103 22-23 0.7163612441070981 1103 24-25 0.8170310011742217 1103 26-27 0.82214687102082 1103 28-29 0.7825907028583288 1103 30-31 0.8665961470518084 1103 32-33 0.8295823386228562 1103 34-35 0.8171553479830251 1103 36-37 0.7000603931515812 1103 38-39 0.7043351307005494 1103 40-41 0.7900367460725235 1103 42-43 0.6761462870188666 1103 44-45 0.6369731722031631 1103 46-47 0.5516482152929143 1103 48-49 0.6322616095036295 1103 50-51 0.5642249598621802 1103 52-53 0.5564393554543443 1103 54-55 0.619733902082352 1103 56-57 0.4726797158498144 1103 58-59 0.4945330887980006 1103 60-61 0.4972696877415146 1103 62-63 0.44590165559831973 1103 64-65 0.4298959552107675 1103 66-67 0.3523358484150876 1103 68-69 0.3294164875526633 1103 70-71 0.302540903016812 1103 72-73 0.4039395521057898 1103 74-75 0.45049677371149954 1103 76-77 0.4802866285792575 1103 78-79 0.4865916830262691 1103 80-81 0.3570901138394831 1103 82-83 0.3781079804404328 1103 84-85 0.21843067293164964 1103 86-87 0.20270208494470054 1103 88-89 0.29714125184702667 1103 90-91 0.2528211457640488 1103 92-93 0.20628679158427943 1103 94-95 0.22757743446664236 1103 96-97 0.25997743926882677 1103 98-99 0.048914299562991204 1103 100-101 0.12081697664618218 1103 102-103 -0.06771652481073787 1103 104-105 0.09369743364235816 1103 106-107 0.13253890811670388 1103 108-109 0.1292929957673934 1103 110-111 0.19561024989404174 1103 112-113 0.12447121495917202 1103 114-115 0.020990047659878996 1103 116-117 -0.08937171507369257 1103 118-119 -0.06856434879746232 1103 120-121 -0.2776620374144514 1103 122-123 -0.3038431834848545 1103 124-125 -0.3593667623857364 1104 1 0.37756016674985915 1104 2 0.3863121990671061 1104 3 0.43586101999861526 1104 4 0.49822491650139256 1104 5 0.37104800963589923 1104 6 0.38360903942248825 1104 7 0.37394941266011017 1104 8 0.38278104227846654 1104 9 0.6022654607046007 1104 10-11 0.6310146679112378 1104 12-13 0.4879418768287991 1104 14-15 0.6627223211341473 1104 16-17 0.5424085859642602 1104 18-19 0.6247576962423835 1104 20-21 0.6961745188216995 1104 22-23 0.6329527540324023 1104 24-25 0.5794081297911404 1104 26-27 0.6358438574992036 1104 28-29 0.602210238336923 1104 30-31 0.6641970647551787 1104 32-33 0.663983359172299 1104 34-35 0.48866039469228184 1104 36-37 0.4769510137221076 1104 38-39 0.443442677360359 1104 40-41 0.5787691682951746 1104 42-43 0.490579018439 1104 44-45 0.4993390932358466 1104 46-47 0.46687437775014473 1104 48-49 0.5095224099888469 1104 50-51 0.39996462745841654 1104 52-53 0.3234228011931535 1104 54-55 0.3091427349772644 1104 56-57 0.37961193633596224 1104 58-59 0.2831105826503162 1104 60-61 0.18123822739329398 1104 62-63 0.3028444243990407 1104 64-65 0.36092283363485933 1104 66-67 0.3580770982585122 1104 68-69 0.3149105772001519 1104 70-71 0.16368190101922409 1104 72-73 0.20276737064840944 1104 74-75 0.13747652754020834 1104 76-77 0.24153541717125648 1104 78-79 0.28387549202265916 1104 80-81 0.2863916835605451 1104 82-83 0.2106346973434441 1104 84-85 0.21796358037678232 1104 86-87 0.3318668562110929 1104 88-89 0.2994186988911913 1104 90-91 0.30697157791530927 1104 92-93 0.20412669453978438 1104 94-95 0.2249607931818609 1104 96-97 0.2507410713172433 1104 98-99 0.22296544570329502 1104 100-101 0.10929195406619385 1104 102-103 -0.03461030987564584 1104 104-105 0.1681368896130344 1104 106-107 0.19918843377217854 1104 108-109 0.26693103035267995 1104 110-111 0.23148836411910523 1104 112-113 0.19474177922807456 1104 114-115 0.0971887647211176 1104 116-117 0.03945188414309797 1104 118-119 -0.006517184645908003 1104 120-121 0.020818016381955573 1104 122-123 0.0298623075721558 1104 124-125 -0.11278111996041673 1105 1 0.21971411348936698 1105 2 0.18219569267391478 1105 3 0.11037985462334632 1105 4 0.2568447826761897 1105 5 0.28211701561957625 1105 6 0.28515362264364086 1105 7 0.24329277197472265 1105 8 0.3592763688687626 1105 9 0.3467474620893398 1105 10-11 0.4006810566156602 1105 12-13 0.5148157073265551 1105 14-15 0.6367272204397594 1105 16-17 0.5255062823212313 1105 18-19 0.4821318446218612 1105 20-21 0.5121073805037 1105 22-23 0.5502759104333919 1105 24-25 0.5576111198251894 1105 26-27 0.5649541106264593 1105 28-29 0.5832820357360617 1105 30-31 0.5588867594790443 1105 32-33 0.5867418154098658 1105 34-35 0.5138760041177122 1105 36-37 0.3894854243743495 1105 38-39 0.44155466866557447 1105 40-41 0.43789467337686716 1105 42-43 0.3212039594000302 1105 44-45 0.32485714138192634 1105 46-47 0.28168669309305727 1105 48-49 0.2834782560937583 1105 50-51 0.3029962850815764 1105 52-53 0.3407069343843574 1105 54-55 0.3335122518713476 1105 56-57 0.21551305937653353 1105 58-59 0.18817724936415203 1105 60-61 0.22675342397934628 1105 62-63 0.30048012380782296 1105 64-65 0.26738016654882557 1105 66-67 0.2338006462360056 1105 68-69 0.24405019502742675 1105 70-71 0.2010797039028418 1105 72-73 0.16724466306740027 1105 74-75 0.1608173954546004 1105 76-77 0.17680128976454768 1105 78-79 0.24947580732170138 1105 80-81 0.24638378339485456 1105 82-83 0.2843875024683271 1105 84-85 0.2775151920211947 1105 86-87 0.2454762625382294 1105 88-89 0.2728244521788845 1105 90-91 0.23498534252999903 1105 92-93 0.25441736969084516 1105 94-95 0.2502883776380962 1105 96-97 0.28651169833850076 1105 98-99 0.19023698391472976 1105 100-101 0.10303338125429917 1105 102-103 -5.781261644202118E-4 1105 104-105 0.17915360520317591 1105 106-107 0.12819750251140505 1105 108-109 0.09577480767202218 1105 110-111 0.1031718777505155 1105 112-113 0.007888266979989567 1105 114-115 0.0392369100926615 1105 116-117 -0.08523089749356672 1105 118-119 -0.0050103863640593715 1105 120-121 -0.1413398715956049 1105 122-123 -0.21069411103827562 1105 124-125 -0.30867188398391576 1106 1 0.4098257622674062 1106 2 0.4481448362814362 1106 3 0.44149944686408915 1106 4 0.46486675984765924 1106 5 0.3902104555766428 1106 6 0.3229899427659575 1106 7 0.2661097816656266 1106 8 0.320010449049839 1106 9 0.5078436719818242 1106 10-11 0.525066893740707 1106 12-13 0.3903723387892697 1106 14-15 0.6325672865535807 1106 16-17 0.5976878665935743 1106 18-19 0.6441752589090868 1106 20-21 0.493293490752464 1106 22-23 0.5805362723223126 1106 24-25 0.4954180292704038 1106 26-27 0.6094135088427208 1106 28-29 0.5987879552797963 1106 30-31 0.7310806841963142 1106 32-33 0.6386993453601733 1106 34-35 0.5737849514132662 1106 36-37 0.63958436292409 1106 38-39 0.5931101666992689 1106 40-41 0.6943806306385056 1106 42-43 0.6084203764055971 1106 44-45 0.4002162397296729 1106 46-47 0.5637527157633571 1106 48-49 0.4805466049594287 1106 50-51 0.4259996913883697 1106 52-53 0.5096861087011035 1106 54-55 0.4828448788897859 1106 56-57 0.4705417691851608 1106 58-59 0.4278300227824019 1106 60-61 0.39337518633247726 1106 62-63 0.49641181880018337 1106 64-65 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2216 30-31 -2.0074644102735206 2216 32-33 -1.9103036844065144 2216 34-35 -1.6083810425759069 2216 36-37 -1.66692617820258 2216 38-39 -2.039375046499849 2216 40-41 -1.8420709807216227 2216 42-43 -1.6899637625244495 2216 44-45 -1.6337561988302411 2216 46-47 -1.571535259928929 2216 48-49 -1.6989337815739134 2216 50-51 -1.6112120796052807 2216 52-53 -1.7386625662597766 2216 54-55 -1.6094554172158766 2216 56-57 -1.7034210238709449 2216 58-59 -1.8344534045634262 2216 60-61 -1.8813569856845191 2216 62-63 -1.955314000143538 2216 64-65 -1.7424261709250004 2216 66-67 -2.0359792709250115 2216 68-69 -1.863452914507505 2216 70-71 -1.8898092198217462 2216 72-73 -1.7572997166092144 2216 74-75 -1.7259545747335991 2216 76-77 -1.6646218577027554 2216 78-79 -1.9097881989716186 2216 80-81 -1.6948438594825248 2216 82-83 -1.6927257394948256 2216 84-85 -1.7457015884049731 2216 86-87 -1.7464328008026655 2216 88-89 -1.7623321089272004 2216 90-91 -1.8540045633311166 2216 92-93 -1.902927960517605 2216 94-95 -1.7123785130845874 2216 96-97 -1.7091623143010217 2216 98-99 -1.7433724877951917 2216 100-101 -1.7073032146422094 2216 102-103 -1.7483324164133869 2216 104-105 -2.0016046246038712 2216 106-107 -1.8203707812256802 2216 108-109 -1.7203778435106436 2216 110-111 -1.7369455765443291 2216 112-113 -1.7225301660501344 2216 114-115 -1.7462425593997182 2216 116-117 -1.8155051982965134 2216 118-119 -1.7185735151851027 2216 120-121 -1.6844771233610984 2216 122-123 -1.72509302156028 2216 124-125 -1.5995048722810878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42215.0 3 8121.0 4 4957.0 5 4190.0 6 4565.0 7 5482.0 8 5967.0 9 6705.0 10 7463.0 11 8157.0 12 8715.0 13 9049.0 14 8813.0 15 8439.0 16 7885.0 17 7131.0 18 6764.0 19 6743.0 20 6952.0 21 7328.0 22 8746.0 23 11337.0 24 15368.0 25 20598.0 26 23951.0 27 26774.0 28 31533.0 29 37781.0 30 45533.0 31 56472.0 32 72203.0 33 94766.0 34 131080.0 35 200628.0 36 388343.0 37 646256.0 38 235933.0 39 406.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.781190787842576 18.469266035850254 12.564732625963423 25.18481055034375 2 15.668430988795572 20.098653300580846 38.72434427177068 25.5085714388529 3 18.17395109888306 24.6431997797871 29.938159576090168 27.244689545239677 4 12.308692918230813 16.192092865515384 37.710198276583284 33.78901593967052 5 12.949003673635747 38.77260257703343 35.0579837021215 13.220410047209324 6 32.607018281075874 37.122241113322154 17.14060632182486 13.130134283777114 7 28.636158984422615 32.44740782454087 21.737274584882655 17.17915860615386 8 26.898794384137414 34.64082272431854 20.396069885690256 18.064313005853794 9 26.864698254749932 15.449126520397833 19.52540964104151 38.16076558381073 10-11 24.739885173016333 26.2964137369594 28.422039764409035 20.54166132561523 12-13 25.47301371123006 23.82991049210787 27.828069104653462 22.869006692008607 14-15 22.297769256060366 25.510482207166792 24.852080586901053 27.339667949871792 16-17 22.823145835151166 28.636793373590976 26.1614476559204 22.37861313533746 18-19 23.08269400082533 27.515671751716724 26.959146062412646 22.4424881850453 20-21 23.04104509079215 27.034752777062515 27.114092601358987 22.81010953078635 22-23 23.130421719667044 26.8890309168736 27.011863634544103 22.968683728915256 24-25 22.9442386238058 27.212079615031197 26.887546669461248 22.95613509170175 26-27 22.433542762330845 27.481225377775036 26.713374025736876 23.371857834157243 28-29 22.932696394573394 27.606086870087072 26.538212952297506 22.923003783042024 30-31 22.652040056761322 27.131326897085245 26.527664397563132 23.6889686485903 32-33 22.289775606074542 27.409922303988427 26.478105394822983 23.82219669511405 34-35 23.510404773379395 27.326683999522338 26.19527326234224 22.967637964756026 36-37 23.643734584059715 26.619831893162925 26.231161293688615 23.505272229088746 38-39 22.824987946084814 27.07909797134407 26.401267640095277 23.69464644247584 40-41 23.182217152757335 26.7145257335438 26.817546715326333 23.285710398372533 42-43 22.98979975752582 26.709536305797226 26.392939058290732 23.907724878386222 44-45 22.775650571916774 27.04682156763353 26.91703777200961 23.26049008844009 46-47 23.59638949664391 27.449590066145785 25.91381955084374 23.040200886366566 48-49 22.861952846809025 27.43606604271304 26.012110559340883 23.689870551137044 50-51 23.182302913307808 27.254695506643355 26.363292492541657 23.199709087507177 52-53 23.56677240938218 26.79705269729382 26.450184206419138 23.18599068690486 54-55 22.612765054044196 27.333170510324194 26.63737201864099 23.416692416990625 56-57 22.826036601574113 27.513271657550533 26.45644157209828 23.204250168777072 58-59 22.62954502214912 26.719946890690082 26.671866053714705 23.97864203344609 60-61 22.18609276527302 27.22227269701511 26.95967134013274 23.63196319757913 62-63 22.537904215492542 27.059423582011615 27.265397986672717 23.137274215823126 64-65 22.30359691326901 27.18887449102087 26.73965950966189 23.767869086048226 66-67 22.165102399632257 27.35140437700912 26.68833566695258 23.795157556406043 68-69 22.97294987585909 26.566739232125506 27.2124293836134 23.247881508402003 70-71 22.668596824460142 26.611462114768674 26.69907934441802 24.020861716353163 72-73 22.47964004833359 26.92647883313914 26.612688309734388 23.98119280879288 74-75 22.469081551210728 27.332126873081265 26.620709631614325 23.57808194409368 76-77 22.853370520820473 26.85014832491015 26.861122861511465 23.435358292757908 78-79 22.822915750704865 26.8162131029971 26.97060069028006 23.390270456017976 80-81 22.985675514971714 26.934848960521308 26.70211206093933 23.377363463567647 82-83 22.81634007888122 27.003615726408388 26.819928369127034 23.360115825583367 84-85 22.53225669342974 27.214250471251095 26.903276471358144 23.350216363961017 86-87 22.865668808831572 27.172405293762054 26.573789544891905 23.388136352514465 88-89 22.701315127792927 27.22741392385591 26.472153277743942 23.59911767060722 90-91 22.47010798187948 27.13391048406539 26.832786407452584 23.563195126602547 92-93 22.71155406099824 26.64014934378734 27.177474833318456 23.47082176189597 94-95 22.326303025287338 26.353984975662588 27.531387659749402 23.788324339300672 96-97 22.751871285427587 25.96526034278189 27.404335283254277 23.878533088536248 98-99 22.429421666398675 26.401643482572858 27.09285968891223 24.076075162116243 100-101 22.374162232230283 26.097154749273493 27.543820747270185 23.98486227122604 102-103 22.01425070884728 25.898472919875072 27.709204399890613 24.378071971387037 104-105 21.841837528684398 26.402151003852776 27.23652856475905 24.519482902703775 106-107 22.181315055701468 26.31359608778553 26.810112131616222 24.69497672489678 108-109 22.233839004442014 26.047174580671395 27.0116573622463 24.70732905264029 110-111 22.219033802323413 26.234396389009518 27.340195882425565 24.206373926241504 112-113 22.239173916739492 26.55514680582249 27.55019428231138 23.65548499512664 114-115 22.009022212796754 26.85739897687684 27.212210909910333 23.921367900416072 116-117 21.979468932294076 26.640642446510583 27.412328490786386 23.96756013040896 118-119 21.783459524231105 26.91165542224135 26.997847558935646 24.3070374945919 120-121 21.69956855282456 27.15287822249971 27.243899789460617 23.903653435215112 122-123 21.73906981116545 27.179077295639885 27.240068565702124 23.841784327492537 124-125 21.672105266708797 27.05760118382318 27.348654368159114 23.921639181308908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 347.0 1 322.5 2 281.5 3 246.5 4 198.0 5 168.0 6 156.5 7 126.0 8 124.5 9 136.0 10 123.0 11 124.5 12 132.5 13 147.5 14 147.5 15 159.5 16 199.5 17 248.0 18 310.0 19 382.5 20 501.0 21 710.5 22 1103.0 23 1727.0 24 2688.0 25 4321.0 26 6826.5 27 10074.5 28 13216.0 29 16845.5 30 21572.0 31 27871.0 32 39252.0 33 44290.5 34 42127.5 35 44751.5 36 51458.5 37 58167.5 38 62251.5 39 66449.0 40 69889.0 41 72123.5 42 73086.5 43 74865.5 44 77120.5 45 81438.5 46 83258.5 47 82053.5 48 80894.0 49 78809.0 50 79257.5 51 78759.0 52 78818.5 53 81924.0 54 85006.5 55 80695.0 56 73037.5 57 67010.5 58 62684.0 59 54982.5 60 42046.0 61 34751.0 62 30718.0 63 22319.0 64 14512.5 65 10814.0 66 7887.0 67 6258.0 68 5225.0 69 4071.5 70 2930.0 71 1853.5 72 1296.0 73 833.5 74 604.0 75 506.5 76 337.0 77 170.0 78 104.0 79 73.5 80 42.0 81 30.0 82 24.0 83 19.0 84 16.5 85 13.5 86 12.0 87 8.5 88 3.5 89 4.0 90 6.0 91 4.0 92 3.0 93 3.0 94 2.0 95 2.5 96 3.0 97 3.0 98 4.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04218860826617863 2 0.009445210805860887 3 0.002068950938426671 4 0.004092924682539719 5 0.021723984853480042 6 0.03004476580150035 7 0.0027885860474446433 8 1.799087772544931E-4 9 0.0 10-11 1.799087772544931E-4 12-13 0.0011469184549973935 14-15 4.4977194313623275E-4 16-17 1.3493158294086984E-4 18-19 6.746579147043492E-5 20-21 6.746579147043492E-5 22-23 0.0 24-25 0.0 26-27 8.995438862724655E-5 28-29 2.698631658817397E-4 30-31 1.799087772544931E-4 32-33 2.0239737441130477E-4 34-35 8.320780948020307E-4 36-37 4.497719431362328E-5 38-39 2.2488597156811638E-4 40-41 6.971465118611608E-4 42-43 8.770552891156539E-4 44-45 0.0016191789952904382 46-47 1.1244298578405819E-4 48-49 0.0 50-51 0.0 52-53 0.0012593614407814518 54-55 0.003418266767835369 56-57 8.995438862724655E-5 58-59 4.4977194313623275E-4 60-61 7.646123033315956E-4 62-63 6.521693175475375E-4 64-65 0.0011694070521542051 66-67 0.002248859715681164 68-69 0.0031034264076400067 70-71 0.0018890421611721777 72-73 0.0022713483128379754 74-75 0.0018215763697017426 76-77 9.220324834292771E-4 78-79 0.002181393924210729 80-81 0.0010344754692133355 82-83 2.698631658817397E-4 84-85 5.397263317634793E-4 86-87 0.0023837912986220335 88-89 0.0064317387868481285 90-91 5.847035260771026E-4 92-93 0.0010119868720565239 94-95 0.0010344754692133355 96-97 4.94749137449856E-4 98-99 9.670096777429004E-4 100-101 0.0013493158294086982 102-103 9.445210805860888E-4 104-105 6.746579147043491E-4 106-107 0.007106396701552478 108-109 0.0014842474123495683 110-111 0.0011469184549973935 112-113 0.0017091333839176846 114-115 0.0026311658673469616 116-117 0.0015966903981336265 118-119 0.04445995657901661 120-121 0.011221809981249008 122-123 0.003598175545089862 124-125 0.001978996549799424 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2223349.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.17225575727234 #Duplication Level Percentage of deduplicated Percentage of total 1 81.84131245519951 49.245763845651034 2 10.042716270725977 12.085857838796876 3 3.3167558610857544 5.987300458730522 4 1.5488218136690863 3.7278440917815474 5 0.8548170047733752 2.571813371844452 6 0.5184641391891035 1.871829407055647 7 0.3451852832097084 1.4539404001458744 8 0.2343329988434422 1.1280276111020966 9 0.16860373313819502 0.9130740256820129 >10 0.9734930408254048 11.150206079596826 >50 0.10289931517527087 4.304319336231142 >100 0.05163826512218912 5.192589551112019 >500 9.598190430065425E-4 0.3674339822699498 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.497719431362328E-5 0.0 0.0 0.0 0.0 6 1.3493158294086984E-4 0.0 0.0 0.0 0.0 7 1.3493158294086984E-4 0.0 0.0 0.0 0.0 8 1.3493158294086984E-4 0.0 0.0 0.0 0.0 9 1.3493158294086984E-4 0.0 0.0 0.0 0.0 10-11 1.3493158294086984E-4 0.0 0.0 0.0 0.0 12-13 1.3493158294086984E-4 0.0 0.0 0.0 0.0 14-15 1.3493158294086984E-4 0.0 0.0 0.0 0.0 16-17 1.3493158294086984E-4 0.0 0.0 0.0 0.0 18-19 2.698631658817397E-4 0.0 0.0 0.0 4.497719431362328E-5 20-21 4.047947488226095E-4 0.0 0.0 4.497719431362328E-5 1.799087772544931E-4 22-23 4.047947488226095E-4 0.0 0.0 1.3493158294086984E-4 1.799087772544931E-4 24-25 4.047947488226095E-4 0.0 0.0 2.2488597156811638E-4 1.799087772544931E-4 26-27 4.272833459794211E-4 0.0 0.0 6.521693175475375E-4 1.799087772544931E-4 28-29 6.296807203907259E-4 0.0 0.0 0.003058449213326383 1.799087772544931E-4 30-31 6.746579147043492E-4 0.0 0.0 0.01288596617085307 1.799087772544931E-4 32-33 6.971465118611608E-4 0.0 0.0 0.036746367754230215 1.799087772544931E-4 34-35 7.421237061747841E-4 0.0 0.0 0.07232332845630624 2.2488597156811638E-4 36-37 8.09589497645219E-4 0.0 0.0 0.13189562232470026 2.2488597156811638E-4 38-39 8.545666919588423E-4 0.0 0.0 0.22787695498997235 2.698631658817397E-4 40-41 8.545666919588423E-4 0.0 0.0 0.3295479027359177 2.698631658817397E-4 42-43 9.220324834292772E-4 0.0 0.0 0.4352892865672461 2.698631658817397E-4 44-45 9.89498274899712E-4 0.0 0.0 0.5651159579535197 2.698631658817397E-4 46-47 0.0012143842464678286 0.0 0.0 0.7225361380512012 2.698631658817397E-4 48-49 0.0012143842464678286 0.0 0.0 0.8878723043480803 2.698631658817397E-4 50-51 0.0012143842464678286 0.0 0.0 1.0699174983324706 2.698631658817397E-4 52-53 0.0012143842464678286 0.0 0.0 1.2407633709327686 2.698631658817397E-4 54-55 0.0012368728436246402 0.0 0.0 1.4209420113531435 2.698631658817397E-4 56-57 0.0014392702180359449 0.0 0.0 1.6382493256794142 2.698631658817397E-4 58-59 0.0014392702180359449 4.497719431362328E-5 0.0 1.8699493421860445 2.698631658817397E-4 60-61 0.0014842474123495683 8.995438862724655E-5 0.0 2.12883807265526 2.698631658817397E-4 62-63 0.0015742018009768147 8.995438862724655E-5 0.0 2.382104653835273 3.598175545089862E-4 64-65 0.0015742018009768147 8.995438862724655E-5 0.0 2.6370803683992032 3.598175545089862E-4 66-67 0.001619178995290438 8.995438862724655E-5 0.0 2.933817407883333 3.598175545089862E-4 68-69 0.0016641561896040612 8.995438862724655E-5 0.0 3.2647596036429727 3.598175545089862E-4 70-71 0.0017091333839176846 8.995438862724655E-5 0.0 3.6168860579243294 3.598175545089862E-4 72-73 0.0017091333839176846 8.995438862724655E-5 0.0 3.9879479110117213 3.823061516657979E-4 74-75 0.0017091333839176846 8.995438862724655E-5 0.0 4.347023341814533 4.047947488226095E-4 76-77 0.0017091333839176846 8.995438862724655E-5 0.0 4.739044567452074 4.047947488226095E-4 78-79 0.0017316219810744962 8.995438862724655E-5 0.0 5.185488198209098 4.047947488226095E-4 80-81 0.0019340193554858009 8.995438862724655E-5 0.0 5.658333442028219 4.047947488226095E-4 82-83 0.001978996549799424 8.995438862724655E-5 0.0 6.1616282463976635 4.047947488226095E-4 84-85 0.0020239737441130473 8.995438862724655E-5 0.0 6.658896106729083 4.047947488226095E-4 86-87 0.002068950938426671 8.995438862724655E-5 0.0 7.171478701724291 4.047947488226095E-4 88-89 0.002068950938426671 8.995438862724655E-5 0.0 7.710372955392968 4.047947488226095E-4 90-91 0.002248859715681164 8.995438862724655E-5 0.0 8.302385275546035 4.047947488226095E-4 92-93 0.002383791298622034 8.995438862724655E-5 0.0 8.904022715282217 4.047947488226095E-4 94-95 0.002383791298622034 1.3493158294086984E-4 0.0 9.49992556274341 4.047947488226095E-4 96-97 0.002383791298622034 1.3493158294086984E-4 0.0 10.126300459352086 4.722605402930444E-4 98-99 0.0025187228815629036 1.3493158294086984E-4 0.0 10.769002077496605 4.94749137449856E-4 100-101 0.00260867727019015 1.3493158294086984E-4 0.0 11.469769253500012 4.94749137449856E-4 102-103 0.0027211202559742085 1.3493158294086984E-4 0.0 12.200716126887862 4.94749137449856E-4 104-105 0.0031034264076400062 1.3493158294086984E-4 0.0 12.92961653793444 4.94749137449856E-4 106-107 0.0031933807962672527 1.3493158294086984E-4 0.0 13.656268089265339 4.94749137449856E-4 108-109 0.003238357990580876 1.3493158294086984E-4 0.0 14.426952313829272 4.94749137449856E-4 110-111 0.0032608465877376875 1.3493158294086984E-4 0.0 15.233325942081066 4.94749137449856E-4 112-113 0.003283335184894499 1.3493158294086984E-4 0.0 16.059579490219484 4.94749137449856E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1610 0.0 49.547813 1 GTATCAA 2520 0.0 43.939785 1 CAACGCA 2730 0.0 39.020187 5 ATCAACG 2775 0.0 38.173832 3 TCAACGC 2855 0.0 37.104164 4 GTACATG 7565 0.0 35.254505 1 AACGCAG 3070 0.0 34.888657 6 TACATGG 7555 0.0 34.187237 2 TAAGGTG 1655 0.0 33.44136 5 TATCAAC 3235 0.0 33.113644 2 ACATGGG 7615 0.0 32.98005 3 AGGTAAG 1440 0.0 31.822695 2 GTAAGGT 1690 0.0 29.580217 4 GGTAAGG 1675 0.0 29.134516 3 CGCAGAG 3785 0.0 27.97861 8 ACGCAGA 3870 0.0 27.210361 7 GAGTACA 5420 0.0 27.019753 1 CATGGGA 3395 0.0 26.294216 4 CATGGGG 4685 0.0 25.786667 4 TAAGGTA 900 0.0 24.466282 4 >>END_MODULE