FastQCFastQC Report
Thu 26 May 2016
SRR1364358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2223349
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22790.10250302584074746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.066.885551
GTATCAA44600.051.6745531
ATCAACG56000.040.1889463
CAACGCA57600.038.969225
TCAACGC58650.038.4745944
AACGCAG60300.037.3990826
TATCAAC61100.036.834392
TAAGGTG16400.034.8522075
ACGCAGA70400.032.1997347
GTACATG70200.032.1516081
CGCAGAG71000.031.7600258
TACATGG70900.031.4911042
GTAAGGT17650.030.359924
ACATGGG71950.030.286783
AGGTAAG16150.029.8617552
GGTAAGG17300.029.2533573
GTGTAAG11250.028.0558831
CATGGGG45800.025.6096484
GCAGAGT92350.024.2242819
GAGTACT51600.024.0412112-13
Produced by FastQC (version 0.11.5)