FastQCFastQC Report
Thu 26 May 2016
SRR1364357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626674
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17400.063.642791
GTATCAA30550.053.982631
ATCAACG36000.044.3082163
TCAACGC36550.043.3157844
CAACGCA37350.042.3880045
AACGCAG39250.040.3311426
TATCAAC40900.039.7287252
GTACATG94650.037.175121
TACATGG96850.035.5216832
ACATGGG97750.034.3411063
ACGCAGA48450.032.786567
CGCAGAG50750.031.0662068
CATGGGA68000.030.1968824
CATGGGG35850.028.2235554
GCAGAGT59200.026.1294359
ATGGGAG30250.024.3976655
ATGGGAT27950.024.0629355
GAGTACA69150.024.0213581
GGACCGA3455.2750693E-1122.42456
CAGAGTA56150.022.30389410-11
Produced by FastQC (version 0.11.5)