##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364357_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1626674 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.972655860977675 31.0 31.0 34.0 28.0 34.0 2 31.03666684289538 33.0 31.0 34.0 27.0 34.0 3 31.201292944990822 33.0 31.0 34.0 27.0 34.0 4 34.59672989179147 37.0 35.0 37.0 32.0 37.0 5 34.68259958664121 37.0 35.0 37.0 32.0 37.0 6 34.74513332112027 37.0 35.0 37.0 32.0 37.0 7 34.66429229212491 37.0 35.0 37.0 32.0 37.0 8 34.614440877520636 37.0 35.0 37.0 32.0 37.0 9 36.14346759092479 39.0 37.0 39.0 32.0 39.0 10-11 36.057915415135426 39.0 37.0 39.0 31.0 39.0 12-13 36.070093024170795 39.0 37.0 39.0 31.0 39.0 14-15 37.356710994335685 40.0 38.0 41.0 31.0 41.0 16-17 37.35954161682058 40.0 38.0 41.0 31.0 41.0 18-19 37.36359713132441 40.0 38.0 41.0 31.0 41.0 20-21 37.389383183108606 40.0 38.0 41.0 31.0 41.0 22-23 37.35402975642323 40.0 38.0 41.0 31.0 41.0 24-25 37.36209344957871 40.0 38.0 41.0 31.0 41.0 26-27 37.318863214141246 40.0 38.0 41.0 31.0 41.0 28-29 37.323599258364 40.0 38.0 41.0 31.0 41.0 30-31 37.23013215924026 40.0 37.5 41.0 30.0 41.0 32-33 37.191777823952435 40.0 37.0 41.0 30.0 41.0 34-35 37.173507414515754 40.0 37.0 41.0 30.0 41.0 36-37 37.11463237255897 40.0 37.0 41.0 30.0 41.0 38-39 37.02953787913251 40.0 37.0 41.0 30.0 41.0 40-41 36.963731515964476 40.0 37.0 41.0 30.0 41.0 42-43 36.83669899438978 40.0 37.0 41.0 30.0 41.0 44-45 36.71862985453754 40.0 36.5 41.0 30.0 41.0 46-47 36.60515659560551 40.0 36.0 41.0 30.0 41.0 48-49 36.46537413151006 40.0 36.0 41.0 30.0 41.0 50-51 36.350589915373334 40.0 36.0 41.0 29.0 41.0 52-53 36.195807211524865 39.5 35.0 41.0 29.0 41.0 54-55 36.01092474583106 39.0 35.0 41.0 29.0 41.0 56-57 35.82175377488052 39.0 35.0 41.0 28.0 41.0 58-59 35.599298015459766 39.0 35.0 41.0 28.0 41.0 60-61 35.3451595095268 38.0 34.5 40.0 28.0 41.0 62-63 35.08010209790038 38.0 34.0 40.0 28.0 41.0 64-65 34.74735042178089 37.0 34.0 40.0 27.0 41.0 66-67 34.42922951986692 37.0 34.0 40.0 26.5 41.0 68-69 34.104013772888734 36.0 34.0 39.0 26.0 41.0 70-71 33.75839043348575 36.0 33.5 39.0 26.0 41.0 72-73 33.391438604170226 35.0 33.0 38.5 26.0 40.0 74-75 33.035371561849516 35.0 33.0 37.5 26.0 39.5 76-77 32.8013431086991 35.0 33.0 37.0 26.0 39.0 78-79 32.64420498514146 35.0 33.0 37.0 26.0 39.0 80-81 32.37490394510516 35.0 33.0 36.0 26.0 38.5 82-83 32.08630678304319 35.0 33.0 36.0 25.0 37.0 84-85 31.841172847171592 35.0 33.0 35.5 25.0 37.0 86-87 31.627263668073628 35.0 33.0 35.0 25.0 36.5 88-89 31.377307008042177 35.0 32.0 35.0 24.0 36.0 90-91 31.25872178445097 35.0 32.0 35.0 24.0 36.0 92-93 31.112880023901532 35.0 32.0 35.0 23.0 36.0 94-95 31.01253816068862 35.0 32.0 35.0 23.0 35.0 96-97 30.908772747335973 35.0 32.0 35.0 22.0 35.0 98-99 30.785421664082662 35.0 32.0 35.0 20.0 35.0 100-101 30.706671404350224 35.0 32.0 35.0 20.0 35.0 102-103 29.691274649991332 33.5 30.0 34.5 18.5 35.0 104-105 30.402213965428842 34.0 31.0 35.0 19.0 35.0 106-107 30.749838320401015 35.0 32.0 35.0 20.0 35.0 108-109 30.792427984955808 35.0 32.0 35.0 20.0 35.0 110-111 30.74024113006048 35.0 32.0 35.0 19.0 35.0 112-113 30.657472548279497 35.0 32.0 35.0 18.5 35.0 114-115 30.561283022904405 35.0 32.0 35.0 17.5 35.0 116-117 30.427963439509085 35.0 32.0 35.0 12.5 35.0 118-119 30.264658130639575 35.0 32.0 35.0 4.5 35.0 120-121 30.092217309676062 35.0 32.0 35.0 2.0 35.0 122-123 29.913779282142578 35.0 32.0 35.0 2.0 35.0 124-125 29.078923619606634 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13900842697579563 1101 2 0.07669296042410068 1101 3 0.20820434070071414 1101 4 0.20482308178926445 1101 5 0.2710175930304288 1101 6 0.2205296994088073 1101 7 0.27962783453642714 1101 8 0.06141436749387452 1101 9 0.31700945254544166 1101 10-11 0.4089454866322697 1101 12-13 0.38640590840366684 1101 14-15 0.43729025449115255 1101 16-17 0.30855782797862474 1101 18-19 0.21530862987065547 1101 20-21 0.4339319940412665 1101 22-23 0.4228637479650885 1101 24-25 0.4298343295682088 1101 26-27 0.5698135871423986 1101 28-29 0.6070739035365662 1101 30-31 0.5886173777154937 1101 32-33 0.5290319402684176 1101 34-35 0.46319278047692336 1101 36-37 0.4263424332800696 1101 38-39 0.5075481855418502 1101 40-41 0.4530260272674198 1101 42-43 0.6165468640565663 1101 44-45 0.561647669455283 1101 46-47 0.6036179240453734 1101 48-49 0.43256879551411487 1101 50-51 0.4388834754056177 1101 52-53 0.3786734644889478 1101 54-55 0.3997109813729196 1101 56-57 0.3694691953072109 1101 58-59 0.403840659531447 1101 60-61 0.3361529408764028 1101 62-63 0.419460952488663 1101 64-65 0.4779514976475099 1101 66-67 0.4229233707230122 1101 68-69 0.46802328278322136 1101 70-71 0.37099810427242375 1101 72-73 0.4381482251730091 1101 74-75 0.4249871791917528 1101 76-77 0.4191568802153043 1101 78-79 0.41179835572045675 1101 80-81 0.4601789991594032 1101 82-83 0.3931045615055737 1101 84-85 0.43073714904327787 1101 86-87 0.41734577170160136 1101 88-89 0.43300803643645835 1101 90-91 0.3610412327848245 1101 92-93 0.3402284375996416 1101 94-95 0.4471784160045047 1101 96-97 0.3282291228984455 1101 98-99 0.24832682839446463 1101 100-101 0.21056086826589393 1101 102-103 -0.004239049597209998 1101 104-105 0.20343035318433778 1101 106-107 0.2398824117072671 1101 108-109 0.26676768665947037 1101 110-111 0.24852781906682253 1101 112-113 0.2557843254393184 1101 114-115 0.17409907928581347 1101 116-117 0.13909269261755597 1101 118-119 0.1508783583051141 1101 120-121 0.04240342674817654 1101 122-123 0.04086686704522435 1101 124-125 -0.0851427572306207 1102 1 -0.10474177367706616 1102 2 -0.15802974740972076 1102 3 -0.0010902331371731577 1102 4 -0.010574321398692632 1102 5 -8.487443579525689E-4 1102 6 0.020559979350906588 1102 7 0.05586643065585406 1102 8 -0.007024968150865618 1102 9 0.024855904818657848 1102 10-11 -0.01866843463251655 1102 12-13 -0.08969863844718162 1102 14-15 -0.03389976653112825 1102 16-17 0.16339348736471493 1102 18-19 0.11793010796339587 1102 20-21 0.0854388071159562 1102 22-23 0.10584529772620499 1102 24-25 0.10081512776923773 1102 26-27 0.021985576511795557 1102 28-29 -0.06947315852836056 1102 30-31 0.11948362089570708 1102 32-33 0.11394972215564536 1102 34-35 0.020413274769786938 1102 36-37 -0.105341508684198 1102 38-39 0.11669114914819545 1102 40-41 -0.07277718503794972 1102 42-43 -0.11052822862092881 1102 44-45 -0.1435439371412599 1102 46-47 -0.1223937742576311 1102 48-49 0.01949450948303877 1102 50-51 -0.07892713039848331 1102 52-53 -0.017183358752156153 1102 54-55 -0.020116731721302017 1102 56-57 -0.026866300135615973 1102 58-59 -0.10791194035947171 1102 60-61 -0.13378184847888264 1102 62-63 -0.09334442800792431 1102 64-65 -0.07258881335872758 1102 66-67 -0.04053500216342343 1102 68-69 -0.177270440549691 1102 70-71 -0.1742047967776088 1102 72-73 0.012625128647179906 1102 74-75 0.0028194370842342664 1102 76-77 0.007708693739985506 1102 78-79 0.060820494287852966 1102 80-81 0.06729569781064981 1102 82-83 0.07891928580283292 1102 84-85 0.07152931864323264 1102 86-87 0.018257275149853314 1102 88-89 0.06859182793081331 1102 90-91 -0.01036830904596897 1102 92-93 0.07134623587242572 1102 94-95 -0.018845857421620593 1102 96-97 -0.11518720893744216 1102 98-99 -0.10072766023854385 1102 100-101 -0.09876786260980452 1102 102-103 -0.27475770149769474 1102 104-105 -0.13326988398515027 1102 106-107 -0.14884204660895506 1102 108-109 -0.06576204110350403 1102 110-111 6.508798391600124E-4 1102 112-113 -0.1654400192832597 1102 114-115 -0.28533369283938015 1102 116-117 -0.265356020335787 1102 118-119 -0.44619850450341403 1102 120-121 -0.36770128465994034 1102 122-123 -0.5107642937870551 1102 124-125 -0.3965255593813737 1103 1 0.4309173631239567 1103 2 0.3507454790043383 1103 3 0.4662022630903486 1103 4 0.5572760576777895 1103 5 0.5070794265926111 1103 6 0.4316570328552416 1103 7 0.406968133909551 1103 8 0.453074174257722 1103 9 0.5289228681753215 1103 10-11 0.6107011760336931 1103 12-13 0.5398867414869741 1103 14-15 0.6396263543501632 1103 16-17 0.5366708379479803 1103 18-19 0.54492677912598 1103 20-21 0.588091602320624 1103 22-23 0.6078635849988672 1103 24-25 0.6421846618103686 1103 26-27 0.7179687677549396 1103 28-29 0.6444735643996893 1103 30-31 0.689188762351705 1103 32-33 0.7140637019666798 1103 34-35 0.7058707918210487 1103 36-37 0.6008507558395166 1103 38-39 0.5289824219866119 1103 40-41 0.5616480267041482 1103 42-43 0.5252226841318333 1103 44-45 0.42094347152904277 1103 46-47 0.49379849897348294 1103 48-49 0.5005568814299011 1103 50-51 0.4030085933471952 1103 52-53 0.4677726123009833 1103 54-55 0.46560975678624317 1103 56-57 0.275639514227926 1103 58-59 0.2839346283606048 1103 60-61 0.2212653499600563 1103 62-63 0.1867585630696027 1103 64-65 0.29776050123861353 1103 66-67 0.3300081390644394 1103 68-69 0.3100539421612538 1103 70-71 0.13130872624343937 1103 72-73 0.19322687935618177 1103 74-75 0.2438703675130114 1103 76-77 0.2229259127893286 1103 78-79 0.29225419312863465 1103 80-81 0.302567181350625 1103 82-83 0.3774132546251323 1103 84-85 0.2426344351960772 1103 86-87 0.20874784112186973 1103 88-89 0.3216428558824269 1103 90-91 0.2589924549061102 1103 92-93 0.2070453396201728 1103 94-95 0.23266639954407964 1103 96-97 0.2331283888818767 1103 98-99 0.1516889468550282 1103 100-101 0.13210107453934228 1103 102-103 -0.00417929531683825 1103 104-105 0.0934455382930679 1103 106-107 0.22429189316547138 1103 108-109 0.2282173171440114 1103 110-111 0.09054168038841937 1103 112-113 0.2058919249301958 1103 114-115 0.23585575484180055 1103 116-117 0.11477788199684014 1103 118-119 0.05596223576454662 1103 120-121 -0.1116710212350931 1103 122-123 -0.14065034009821176 1103 124-125 -0.35860450237318275 1104 1 0.26891420828201973 1104 2 0.29541980342590435 1104 3 0.2853416914187612 1104 4 0.3290414351066815 1104 5 0.2934164222484341 1104 6 0.24968684942648878 1104 7 0.24978520886494238 1104 8 0.2929223666917622 1104 9 0.46672251549924937 1104 10-11 0.45615200229989483 1104 12-13 0.3117119493370524 1104 14-15 0.40006825860694306 1104 16-17 0.44715846396580616 1104 18-19 0.4032233585237819 1104 20-21 0.36683902819760306 1104 22-23 0.3902078702638505 1104 24-25 0.48486598639121326 1104 26-27 0.4550219901486585 1104 28-29 0.42970911434232306 1104 30-31 0.48469662964782856 1104 32-33 0.5134116312277186 1104 34-35 0.4479422503654362 1104 36-37 0.4027499674524435 1104 38-39 0.293374265968275 1104 40-41 0.3284989818483126 1104 42-43 0.25841377198634063 1104 44-45 0.1558393221276333 1104 46-47 0.17876351839007754 1104 48-49 0.24595774120922442 1104 50-51 0.14793970346831742 1104 52-53 0.13511450030651417 1104 54-55 0.09734413558881272 1104 56-57 -0.048175661515848844 1104 58-59 0.013137048420283293 1104 60-61 -0.1321550501114146 1104 62-63 -0.04016407538728828 1104 64-65 -0.0039471721746053845 1104 66-67 0.0262984912473172 1104 68-69 -0.037530242879682874 1104 70-71 -0.08815386721126828 1104 72-73 -0.1428829628289563 1104 74-75 -0.14562341052744898 1104 76-77 -0.02699141612193756 1104 78-79 0.051637018078572794 1104 80-81 -0.045507451440172986 1104 82-83 -0.01285055710631866 1104 84-85 -0.01869069544040869 1104 86-87 0.10728337028556112 1104 88-89 0.12261315978053844 1104 90-91 -0.01550676851119448 1104 92-93 -0.00517073875444396 1104 94-95 0.013156669419704059 1104 96-97 -0.0694976814964825 1104 98-99 -0.03269676855106596 1104 100-101 -0.016595875483659484 1104 102-103 -0.13614701276201302 1104 104-105 -0.01855021859814343 1104 106-107 -0.046331855145734124 1104 108-109 0.020360936027461918 1104 110-111 -0.045773040563304335 1104 112-113 -0.0016996661466635032 1104 114-115 0.0010197346643927574 1104 116-117 -0.013719590167866613 1104 118-119 -0.022108378685793184 1104 120-121 -0.09843127669482143 1104 122-123 -0.10860225584404049 1104 124-125 -0.13151441958889976 1105 1 -0.05675130938162809 1105 2 -0.10152691992012208 1105 3 -0.06852522323216448 1105 4 0.07407862550384436 1105 5 0.0687015555997803 1105 6 0.21669076116464936 1105 7 0.0826412347262746 1105 8 0.12351734385196522 1105 9 0.1076103411897904 1105 10-11 0.06055352438426809 1105 12-13 0.11420988436605484 1105 14-15 0.3253696526494281 1105 16-17 0.1811543956551276 1105 18-19 0.041016342626924995 1105 20-21 0.1495527468623834 1105 22-23 0.1811224967273688 1105 24-25 0.07212595614198136 1105 26-27 0.10279627083635035 1105 28-29 0.06161799718537253 1105 30-31 0.17326995796268818 1105 32-33 0.16560333262703608 1105 34-35 0.06005436529641628 1105 36-37 -0.026557220899462664 1105 38-39 -0.14059324772940585 1105 40-41 -0.005245146481293261 1105 42-43 0.048244701027769565 1105 44-45 0.007513899755977604 1105 46-47 0.038824863275543464 1105 48-49 -0.18139502631856885 1105 50-51 -0.07521788620323377 1105 52-53 -0.0767120186359449 1105 54-55 -0.11928544232677041 1105 56-57 -0.12279662488576548 1105 58-59 -0.10593457795295791 1105 60-61 -0.10787424173380344 1105 62-63 -0.2157129199770793 1105 64-65 -0.09917922535197476 1105 66-67 -0.13139827075907817 1105 68-69 -0.18061523311119743 1105 70-71 -0.09903123166943573 1105 72-73 -0.2714058242664592 1105 74-75 -0.21481945009572456 1105 76-77 -0.16701189767334057 1105 78-79 -0.006713277277050622 1105 80-81 -0.050047330201870466 1105 82-83 -0.15170165278403047 1105 84-85 -0.18005339446089152 1105 86-87 -0.21716842879376586 1105 88-89 -0.1544811665497292 1105 90-91 -0.07824382983661238 1105 92-93 -0.10874096217038698 1105 94-95 -0.3089538636944198 1105 96-97 -0.2986269114284532 1105 98-99 -0.31460115375613995 1105 100-101 -0.3389721634856855 1105 102-103 -0.5184623324249991 1105 104-105 -0.36075875677246927 1105 106-107 -0.2759767445014276 1105 108-109 -0.2433263257445617 1105 110-111 -0.27306113543626864 1105 112-113 -0.2851465239390727 1105 114-115 -0.29765295524334334 1105 116-117 -0.343067056114954 1105 118-119 -0.3274482737966906 1105 120-121 -0.5016803253116322 1105 122-123 -0.6897943568009666 1105 124-125 -0.7492074676244798 1106 1 0.17272407105890508 1106 2 0.1272357689190251 1106 3 0.24121873361563217 1106 4 0.20803253134668154 1106 5 0.1823355139984244 1106 6 0.03004626564646884 1106 7 0.026740121642774284 1106 8 0.08550840084532751 1106 9 0.12673287355959673 1106 10-11 0.32047371557131754 1106 12-13 0.11300015568473754 1106 14-15 0.273218116077679 1106 16-17 0.30535317920388394 1106 18-19 0.17204069161983426 1106 20-21 0.09437347738541746 1106 22-23 0.1945369234823744 1106 24-25 0.20644169898861264 1106 26-27 0.42938024996844604 1106 28-29 0.21152983841572137 1106 30-31 0.35107170290063294 1106 32-33 0.363488404075909 1106 34-35 0.22557020891493806 1106 36-37 0.17965118323711238 1106 38-39 0.2089229107180941 1106 40-41 0.17323750764013823 1106 42-43 0.14999652793440532 1106 44-45 0.1619356827263232 1106 46-47 0.15871979160036886 1106 48-49 0.17986831314494367 1106 50-51 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-0.6062092916907176 2216 96-97 -0.7118342670991531 2216 98-99 -0.7437754651934263 2216 100-101 -0.6695552879735089 2216 102-103 -0.6288076679436223 2216 104-105 -0.7371413255659931 2216 106-107 -0.5810000333382206 2216 108-109 -0.582233648535432 2216 110-111 -0.5635169791993313 2216 112-113 -0.5145850191972237 2216 114-115 -0.5675147438561261 2216 116-117 -0.6332531628790363 2216 118-119 -0.5646424725138743 2216 120-121 -0.38441453765763356 2216 122-123 -0.4830696934114087 2216 124-125 -0.5041688557809607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26260.0 3 5152.0 4 3363.0 5 2733.0 6 3059.0 7 3524.0 8 3904.0 9 4073.0 10 4620.0 11 5102.0 12 5640.0 13 5813.0 14 5708.0 15 5450.0 16 5135.0 17 4819.0 18 4523.0 19 4321.0 20 4268.0 21 4574.0 22 5359.0 23 7128.0 24 10179.0 25 14873.0 26 17778.0 27 17944.0 28 20709.0 29 25397.0 30 30714.0 31 37803.0 32 48494.0 33 64280.0 34 89994.0 35 138171.0 36 268791.0 37 501551.0 38 215059.0 39 409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49570314590115 18.82669713897723 12.817928149391406 23.859671565730213 2 15.279544500193973 20.295342021406427 40.10098855163874 24.32412492676086 3 18.562672519257607 25.889231109717635 29.131455831996213 26.416640539028545 4 12.27047574314309 16.52482430831485 37.68787221707506 33.51682773146699 5 13.337621258928396 39.01407636606915 34.17054465673639 13.47775771826607 6 31.726843174584435 36.66837615667291 18.09470574634612 13.510074922396534 7 28.622041943493766 32.72085561628828 21.267698542435003 17.389403897782945 8 26.397638860200455 35.49922787138403 20.085548899839672 18.01758436857584 9 26.925923694606297 16.366893427939463 19.57903058633752 37.12815229111672 10-11 24.608180196075118 27.133335075130365 27.859602962489376 20.39888176630514 12-13 25.19593818257154 24.6729035736395 27.741705247743692 22.38945299604527 14-15 22.630394576399553 25.865226996158096 25.10671350677781 26.39766492066454 16-17 22.699495687663852 29.241170845601488 25.70585130793237 22.35348215880229 18-19 22.69435836212933 28.162943627883415 27.04077586583167 22.101922144155587 20-21 23.667356622996756 27.355836114572508 26.709117321059612 22.26768994137113 22-23 23.417568609321844 27.388431855430163 26.516376360598375 22.67762317464962 24-25 23.02194539286913 27.555306103128224 26.633732388911362 22.789016115091286 26-27 22.92686450407196 27.78518724574246 26.41398314657683 22.873965103608747 28-29 22.82490522819465 27.61136935845336 26.695572336253715 22.86815307709828 30-31 22.885113215886925 27.40173028227589 26.760727891503567 22.95242861033362 32-33 22.78458718168137 27.50628276628601 26.61424675656801 23.094883295464605 34-35 23.154800583032667 27.476982074388918 26.49983063528061 22.868386707297805 36-37 23.26375782732127 27.152059970221448 26.319871098941768 23.264311103515517 38-39 22.881725044054686 27.359887967545998 26.55308708611419 23.205299902285127 40-41 23.143886417916413 27.147046257219525 26.735222064776703 22.973845260087355 42-43 23.068649904574784 27.115853188099713 26.600873627138693 23.21462328018681 44-45 22.83497555721677 27.26842676017548 26.659816211909586 23.23678147069817 46-47 23.05156692573336 27.387412032846193 26.43085808606219 23.130162955358255 48-49 22.864200202376136 27.305532638992204 26.529439826295864 23.300827332335796 50-51 22.879691935815043 27.265020526546806 26.56681670697386 23.288470830664288 52-53 23.098557780974513 27.184537641547713 26.54565182644191 23.171252751035865 54-55 22.82570539968807 27.1073156727002 26.69274715654626 23.374231771065475 56-57 22.745581162028977 27.368516824535 26.6559269477805 23.229975065655523 58-59 23.016473622961392 26.846989016150264 26.699724158875142 23.436813202013205 60-61 22.85023122568826 27.06430210416145 26.79359361143941 23.291873058710884 62-63 22.817285587689877 27.152022716465595 26.814091315249055 23.21660038059547 64-65 22.66653018981655 27.10282700729321 26.733417371105457 23.49722543178478 66-67 22.642774346863444 27.346298162798398 26.42688279428992 23.584044696048245 68-69 22.617262593083726 27.054973266766137 26.84650506846376 23.48125907168638 70-71 22.682682873449874 26.893284578555605 26.77730960185511 23.646722946139416 72-73 22.7427997763479 27.0124462578694 26.562129699160874 23.68262426662183 74-75 22.678837752867565 27.10534954833331 26.502512538099964 23.71330016069916 76-77 22.778315862403478 26.992240823112713 26.693560310440844 23.535883004042958 78-79 22.86098163446725 26.98237587558111 26.707114200244185 23.44952828970746 80-81 22.845213003522883 27.291340523769225 26.574592037692156 23.288854435015736 82-83 22.775709811178086 26.986472322205245 26.73073435526061 23.507083511356054 84-85 22.88452399907295 26.938273168386644 26.57126504687192 23.605937785668488 86-87 22.811373734548308 26.740755882131445 26.82181241422132 23.626057969098927 88-89 22.9512238156687 26.732009789787607 26.73794243550784 23.578823959035848 90-91 22.901131976183223 26.899426650236357 26.764918605112225 23.43452276846819 92-93 22.92084317714183 26.481550812494582 26.825599988196654 23.772006022166934 94-95 22.739317761757906 26.432774386542246 26.960173926888864 23.867733924810985 96-97 22.856032788477556 26.434880113901478 26.91804540444307 23.7910416931779 98-99 22.83302067826733 26.386530186557174 26.754646562479735 24.025802572695763 100-101 22.512153827994748 26.523378137126162 26.99846555616212 23.96600247871697 102-103 22.770718110620567 26.22972789791046 26.973587749796284 24.025966241672688 104-105 22.70557461301071 26.445077643760833 26.882415501702877 23.96693224152558 106-107 22.665934654377796 26.433810114741696 26.906124034891903 23.994131195988604 108-109 22.695288341778113 26.488830390877915 26.601178129722143 24.21470313762183 110-111 22.495537612082245 26.684787737262717 26.801100297144163 24.01857435351088 112-113 22.46844946612227 26.840139146023095 26.9277432296644 23.763668158190228 114-115 22.644256749072163 26.742481853598022 26.6760254784275 23.93723591890232 116-117 22.471792340229854 26.69890691956402 26.85450358914364 23.97479715106248 118-119 22.473063819305487 26.852843594098502 26.561317501129356 24.112775085466655 120-121 22.417948475171507 26.933011082842807 26.72065038690963 23.92839005507606 122-123 22.508805181854903 27.000139554985587 26.617562164983504 23.87349309817601 124-125 22.523634637823537 26.99712535917641 26.516717302804928 23.962522700195127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 677.0 1 534.0 2 349.0 3 273.5 4 214.0 5 180.0 6 163.5 7 162.0 8 156.5 9 153.5 10 138.5 11 121.0 12 135.0 13 149.5 14 165.5 15 197.0 16 219.5 17 269.5 18 357.0 19 438.0 20 576.0 21 840.0 22 1187.5 23 1634.5 24 2276.5 25 3260.0 26 4812.5 27 6873.0 28 8808.5 29 11495.0 30 15428.0 31 18955.0 32 22869.0 33 26709.5 34 30466.0 35 33750.0 36 38498.5 37 44682.5 38 48141.0 39 49647.0 40 50577.5 41 52880.5 42 54864.5 43 56824.5 44 60394.0 45 63280.5 46 63119.5 47 62616.0 48 63530.0 49 63806.5 50 62406.0 51 59622.5 52 59247.5 53 59306.0 54 57751.5 55 53743.5 56 48042.0 57 43492.5 58 41027.0 59 37704.0 60 31293.5 61 25084.5 62 19685.0 63 14899.5 64 11611.5 65 9066.0 66 6533.0 67 5161.0 68 3939.5 69 2593.5 70 1815.5 71 1313.0 72 1029.0 73 773.5 74 613.0 75 510.5 76 353.0 77 186.5 78 93.0 79 63.5 80 39.5 81 27.5 82 25.0 83 19.0 84 15.0 85 13.5 86 10.5 87 6.5 88 6.5 89 7.5 90 5.0 91 2.5 92 3.0 93 4.0 94 3.5 95 1.5 96 0.5 97 0.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044077670141651 2 0.009405695302193309 3 0.0027049058385392524 4 0.006331938667489614 5 0.024713003343017716 6 0.028155610773885856 7 0.0035040825635622138 8 1.2295026538814785E-4 9 0.0 10-11 9.221269904111088E-5 12-13 7.991767250229609E-4 14-15 3.3811322981740655E-4 16-17 2.151629644292587E-4 18-19 6.147513269407393E-5 20-21 6.147513269407393E-5 22-23 0.0 24-25 0.0 26-27 9.221269904111088E-5 28-29 2.151629644292587E-4 30-31 1.8442539808222175E-4 32-33 1.2295026538814785E-4 34-35 4.303259288585174E-4 36-37 0.0 38-39 1.536878317351848E-4 40-41 5.532761942466652E-4 42-43 4.610634952055544E-4 44-45 0.0011065523884933304 46-47 9.221269904111088E-5 48-49 0.0 50-51 0.0 52-53 8.606518577170348E-4 54-55 0.0026434307058451787 56-57 1.2295026538814785E-4 58-59 7.991767250229609E-4 60-61 0.0010143396894522197 62-63 7.684391586759241E-4 64-65 0.0015061407510048109 66-67 0.0026126931394981415 68-69 0.0028893312366214743 70-71 0.0018442539808222176 72-73 0.0024897428741099937 74-75 0.0019672042462103656 76-77 0.0011987650875344414 78-79 0.002459005307762957 80-81 0.0013831904856166633 82-83 5.532761942466652E-4 84-85 4.303259288585174E-4 86-87 0.0019364666798633286 88-89 0.004979485748219987 90-91 3.3811322981740655E-4 92-93 0.0010450772557992567 94-95 8.29914291369998E-4 96-97 3.073756634703696E-4 98-99 8.913894240640719E-4 100-101 0.0015983534500459218 102-103 0.0014446656183107372 104-105 8.606518577170348E-4 106-107 0.0066085767646129456 108-109 0.0017213037154340696 110-111 0.0013831904856166633 112-113 0.0023053174760277718 114-115 0.0026434307058451787 116-117 0.0019979418125574025 118-119 0.04595266168882026 120-121 0.012049126008038487 122-123 0.0046106349520555435 124-125 0.002213104776986661 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1626674.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.0193684840996 #Duplication Level Percentage of deduplicated Percentage of total 1 83.55080075980298 55.99521903262825 2 9.7901921951107 13.122649965085598 3 2.990367207960093 6.012375654391368 4 1.2545263611981736 3.3631025789662807 5 0.6597225287201784 2.210709362477981 6 0.38689461816173665 1.5557659787457867 7 0.23930350846194567 1.122657900920429 8 0.18388416042140204 0.9859040244536982 9 0.1281882807991786 0.7731987863601044 >10 0.7298385095283828 9.168422487132876 >50 0.06266236064687879 2.9010984374434585 >100 0.023424306633827257 2.6626510952217726 >500 1.9520255458800988E-4 0.12624469617247402 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.147513269407393E-5 0.0 16-17 0.0 0.0 0.0 6.147513269407393E-5 0.0 18-19 0.0 0.0 0.0 1.2295026538814785E-4 0.0 20-21 0.0 0.0 0.0 1.2295026538814785E-4 0.0 22-23 0.0 0.0 0.0 2.1516296442925873E-4 0.0 24-25 0.0 0.0 0.0 4.3032592885851746E-4 0.0 26-27 0.0 0.0 0.0 0.0010143396894522197 0.0 28-29 0.0 0.0 0.0 0.00313523176739777 0.0 30-31 0.0 0.0 0.0 0.011157736583974415 0.0 32-33 0.0 0.0 0.0 0.03113715470954844 0.0 34-35 0.0 0.0 0.0 0.059630878713251706 0.0 36-37 0.0 0.0 0.0 0.10893393513389898 0.0 38-39 0.0 0.0 0.0 0.18411802241875141 0.0 40-41 0.0 0.0 0.0 0.27027542088949597 0.0 42-43 0.0 0.0 0.0 0.35409676431786574 0.0 44-45 0.0 0.0 0.0 0.45503893220153513 0.0 46-47 0.0 0.0 0.0 0.5782965732531533 0.0 48-49 0.0 0.0 0.0 0.7108062217752298 0.0 50-51 0.0 0.0 0.0 0.8543506566158923 0.0 52-53 0.0 0.0 0.0 0.9984791052171487 0.0 54-55 0.0 0.0 0.0 1.1524743126158037 0.0 56-57 0.0 0.0 0.0 1.3370226609634137 0.0 58-59 0.0 0.0 0.0 1.5448700846020778 0.0 60-61 0.0 0.0 0.0 1.7662727749997846 0.0 62-63 0.0 0.0 0.0 1.9920094622524243 0.0 64-65 0.0 0.0 0.0 2.219713353751274 0.0 66-67 0.0 0.0 0.0 2.4781240740308137 0.0 68-69 0.0 0.0 0.0 2.769393252735336 0.0 70-71 0.0 0.0 0.0 3.0959798951726034 0.0 72-73 0.0 0.0 0.0 3.4296976530023837 0.0 74-75 0.0 0.0 0.0 3.762247383310977 0.0 76-77 0.0 0.0 0.0 4.112901540197974 0.0 78-79 0.0 0.0 0.0 4.5053587873169425 0.0 80-81 0.0 0.0 0.0 4.940940840020803 0.0 82-83 0.0 0.0 0.0 5.398254352132019 0.0 84-85 0.0 0.0 0.0 5.854676474819171 0.0 86-87 0.0 0.0 0.0 6.320657980640251 0.0 88-89 0.0 0.0 0.0 6.8207889226728895 0.0 90-91 0.0 0.0 0.0 7.3773847740850345 0.0 92-93 0.0 0.0 0.0 7.9578329769824805 0.0 94-95 0.0 0.0 0.0 8.538188967180886 0.0 96-97 0.0 0.0 0.0 9.111260154155042 0.0 98-99 0.0 0.0 0.0 9.712732852433863 0.0 100-101 0.0 0.0 0.0 10.378754440041458 0.0 102-103 0.0 0.0 0.0 11.082337333725135 0.0 104-105 0.0 0.0 0.0 11.786534978735752 0.0 106-107 0.0 0.0 0.0 12.476070804598832 0.0 108-109 0.0 0.0 0.0 13.177194692974744 0.0 110-111 0.0 0.0 0.0 13.917570453575824 0.0 112-113 0.0 0.0 0.0 14.716193902404537 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1740 0.0 63.64279 1 GTATCAA 3055 0.0 53.98263 1 ATCAACG 3600 0.0 44.308216 3 TCAACGC 3655 0.0 43.315784 4 CAACGCA 3735 0.0 42.388004 5 AACGCAG 3925 0.0 40.331142 6 TATCAAC 4090 0.0 39.728725 2 GTACATG 9465 0.0 37.17512 1 TACATGG 9685 0.0 35.521683 2 ACATGGG 9775 0.0 34.341106 3 ACGCAGA 4845 0.0 32.78656 7 CGCAGAG 5075 0.0 31.066206 8 CATGGGA 6800 0.0 30.196882 4 CATGGGG 3585 0.0 28.223555 4 GCAGAGT 5920 0.0 26.129435 9 ATGGGAG 3025 0.0 24.397665 5 ATGGGAT 2795 0.0 24.062935 5 GAGTACA 6915 0.0 24.021358 1 GGACCGA 345 5.2750693E-11 22.4245 6 CAGAGTA 5615 0.0 22.303894 10-11 >>END_MODULE