FastQCFastQC Report
Thu 26 May 2016
SRR1364357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1626674
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32560.2001630320519047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27490.16899513977600922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.10149544407791604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.076.674681
GTATCAA50950.056.3378071
ATCAACG63050.044.7620773
TCAACGC64600.043.688064
CAACGCA64650.043.654275
TATCAAC66300.042.7474632
AACGCAG66750.042.2548836
ACGCAGA82550.034.2352077
CGCAGAG82200.034.0190738
GTACATG88700.032.8308641
TACATGG90650.031.6589262
TGGTATC12950.030.8050542
ACATGGG90250.030.3478243
GCAGAGT93300.029.461639
GAGTACT46000.027.54978612-13
CAGAGTA90550.026.31543210-11
TATCACG1608.697494E-626.0492382
CATGGGG33550.025.9105874
CATGGGA65250.025.732714
GTACTTT49400.025.17188614-15
Produced by FastQC (version 0.11.5)