##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364357_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1626674 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28759358052074 33.0 31.0 34.0 30.0 34.0 2 32.31720615193948 33.0 31.0 34.0 30.0 34.0 3 32.42359071332056 34.0 31.0 34.0 30.0 34.0 4 36.02668143709188 37.0 35.0 37.0 35.0 37.0 5 35.96754297419151 37.0 35.0 37.0 35.0 37.0 6 36.02420214499033 37.0 35.0 37.0 35.0 37.0 7 35.97925214271575 37.0 35.0 37.0 35.0 37.0 8 35.996267844694145 37.0 35.0 37.0 35.0 37.0 9 37.747206262594716 39.0 38.0 39.0 35.0 39.0 10-11 37.70247173066023 39.0 37.5 39.0 35.0 39.0 12-13 37.67853669512146 39.0 37.0 39.0 35.0 39.0 14-15 39.06595513298915 41.0 38.0 41.0 35.5 41.0 16-17 39.06892069339032 41.0 38.0 41.0 35.5 41.0 18-19 39.128199319593236 41.0 38.5 41.0 36.0 41.0 20-21 39.1392039830968 41.0 39.0 41.0 35.5 41.0 22-23 39.0979446404135 41.0 39.0 41.0 35.0 41.0 24-25 39.06535544306973 41.0 39.0 41.0 35.0 41.0 26-27 38.975436073853764 41.0 39.0 41.0 35.0 41.0 28-29 38.89004188915542 40.5 38.0 41.0 35.0 41.0 30-31 38.78616274680729 40.0 38.0 41.0 35.0 41.0 32-33 38.675024006039315 40.0 38.0 41.0 34.5 41.0 34-35 38.582826061030055 40.0 38.0 41.0 34.0 41.0 36-37 38.478142823946285 40.0 38.0 41.0 34.0 41.0 38-39 38.39814861490379 40.0 38.0 41.0 34.0 41.0 40-41 38.29592223149814 40.0 38.0 41.0 34.0 41.0 42-43 38.154281066765684 40.0 38.0 41.0 33.0 41.0 44-45 37.9901298600703 40.0 38.0 41.0 33.0 41.0 46-47 37.761477099898315 40.0 37.5 41.0 33.0 41.0 48-49 37.556586937517906 40.0 37.0 41.0 33.0 41.0 50-51 37.403676151459976 40.0 37.0 41.0 32.0 41.0 52-53 37.25719566428184 40.0 37.0 41.0 32.0 41.0 54-55 37.077925878203004 40.0 36.0 41.0 31.5 41.0 56-57 36.88975387815874 39.5 36.0 41.0 31.0 41.0 58-59 36.67492257207037 39.0 35.5 41.0 31.0 41.0 60-61 36.423101063888645 39.0 35.0 41.0 31.0 41.0 62-63 36.18639045069879 38.5 35.0 41.0 31.0 41.0 64-65 35.8933320382572 38.0 35.0 40.0 30.0 41.0 66-67 35.56381887212804 37.0 35.0 40.0 30.0 41.0 68-69 35.231897417675576 37.0 35.0 39.5 29.0 41.0 70-71 34.96915823330305 36.0 34.5 39.0 29.5 41.0 72-73 34.768575018719176 36.0 34.5 39.0 30.0 41.0 74-75 34.42982582865405 35.5 34.0 38.5 29.5 40.0 76-77 34.10054350164815 35.0 34.0 37.0 29.0 39.0 78-79 33.78266020112204 35.0 34.0 37.0 29.0 39.0 80-81 33.48439638181959 35.0 34.0 36.0 29.0 39.0 82-83 33.21139945680572 35.0 34.0 36.0 29.0 37.0 84-85 32.981381026560946 35.0 34.0 36.0 29.0 37.0 86-87 32.79820295892109 35.0 34.0 35.0 29.0 36.5 88-89 32.648448306175666 35.0 34.0 35.0 29.0 36.0 90-91 32.50678470301978 35.0 34.0 35.0 29.0 36.0 92-93 32.3885560351982 35.0 33.0 35.0 27.5 36.0 94-95 32.30818621309494 35.0 33.0 35.0 27.0 35.5 96-97 32.225312816212714 35.0 33.0 35.0 27.0 35.0 98-99 32.16336955038317 35.0 33.0 35.0 27.0 35.0 100-101 32.10695535798814 35.0 33.0 35.0 27.0 35.0 102-103 31.125682527660736 34.0 31.5 35.0 24.5 35.0 104-105 31.849783054256722 35.0 33.0 35.0 26.0 35.0 106-107 32.13940992479132 35.0 33.5 35.0 27.0 35.0 108-109 32.15829969618989 35.0 34.0 35.0 27.0 35.0 110-111 32.12852790417748 35.0 34.0 35.0 27.0 35.0 112-113 32.06318967414491 35.0 34.0 35.0 27.0 35.0 114-115 31.989513571865047 35.0 34.0 35.0 27.0 35.0 116-117 31.930244166932034 35.0 33.5 35.0 27.0 35.0 118-119 31.82690846475692 35.0 33.0 35.0 27.0 35.0 120-121 31.694262341440265 35.0 33.0 35.0 25.0 35.0 122-123 31.54910602862036 35.0 33.0 35.0 25.0 35.0 124-125 30.867056644416767 34.5 32.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.127890813001315 1101 2 0.1287106994192797 1101 3 0.10764218154643856 1101 4 0.1145383043341397 1101 5 0.21770754140440118 1101 6 0.22845580704100854 1101 7 0.26074870603081024 1101 8 0.26747671545266627 1101 9 0.2234751836998612 1101 10-11 0.25319173356783153 1101 12-13 0.2518274579577664 1101 14-15 0.22512434000113757 1101 16-17 0.26900539314149796 1101 18-19 0.2769162747332672 1101 20-21 0.22750481588349203 1101 22-23 0.2693436820586257 1101 24-25 0.17617815156362582 1101 26-27 0.17850801471926303 1101 28-29 0.2070344226702261 1101 30-31 0.22223043072818882 1101 32-33 0.1983030433921087 1101 34-35 0.17435068670762632 1101 36-37 0.2677644876696945 1101 38-39 0.33960208630180233 1101 40-41 0.3042503469016822 1101 42-43 0.26317846538213274 1101 44-45 0.2236262856450324 1101 46-47 0.2791812431413092 1101 48-49 0.14524535770271996 1101 50-51 0.12318966302792944 1101 52-53 0.16196437638373595 1101 54-55 0.1646137551307092 1101 56-57 0.1259188490000227 1101 58-59 -0.015760331571222252 1101 60-61 0.1104537949347062 1101 62-63 -0.12068382800544697 1101 64-65 -0.11445249522876821 1101 66-67 -0.11890410008105334 1101 68-69 -0.03400652667465209 1101 70-71 0.010981930089336345 1101 72-73 -0.00845885549240677 1101 74-75 0.02035398396481014 1101 76-77 -0.06012508049960985 1101 78-79 -0.17784012927491233 1101 80-81 -0.12263611215085746 1101 82-83 -0.10438416750049839 1101 84-85 -0.38138274690642504 1101 86-87 -0.2885246243238697 1101 88-89 -0.32161003361837004 1101 90-91 -0.7925142750546996 1101 92-93 -0.6626232357666808 1101 94-95 -0.4897642863152356 1101 96-97 -0.2828736045547622 1101 98-99 -0.06362323567454808 1101 100-101 -0.26769885616992894 1101 102-103 -0.11787086313021078 1101 104-105 -0.07354903401679991 1101 106-107 -0.021400086701952148 1101 108-109 0.0550689344982942 1101 110-111 -0.021227522044178215 1101 112-113 -0.046175403725754904 1101 114-115 -0.10193678049535748 1101 116-117 -0.08478765058953286 1101 118-119 -0.02273440068670496 1101 120-121 -0.035805931552289394 1101 122-123 0.06126248853061611 1101 124-125 0.18115008709822078 1102 1 0.06638516305534381 1102 2 0.08455163183097625 1102 3 0.07403723514421756 1102 4 0.07475807425920777 1102 5 0.04311104443932834 1102 6 0.013738737662222889 1102 7 0.13112336154384252 1102 8 0.18772485659579274 1102 9 0.2900282508772989 1102 10-11 0.2147218214972071 1102 12-13 0.23477893829782914 1102 14-15 0.23073867283454774 1102 16-17 0.28308723284271764 1102 18-19 0.32715440033818766 1102 20-21 0.2562230427770942 1102 22-23 0.29217100411002406 1102 24-25 0.2561815830564882 1102 26-27 0.10423970250323578 1102 28-29 0.19013528522753376 1102 30-31 0.15340329779485273 1102 32-33 0.1625038537928276 1102 34-35 0.19459114383300147 1102 36-37 0.27764730990766395 1102 38-39 0.19107818084890482 1102 40-41 0.22039297700763427 1102 42-43 0.1906451973570995 1102 44-45 0.1658144903970893 1102 46-47 0.09565312814489602 1102 48-49 0.1163963446165539 1102 50-51 0.0797806643414063 1102 52-53 0.0017461151927449237 1102 54-55 -0.0011570860569563024 1102 56-57 0.05373504589191924 1102 58-59 0.036220820480586724 1102 60-61 0.0053705540405744046 1102 62-63 -0.07641362285445297 1102 64-65 -0.07594047895127432 1102 66-67 -0.09458339444261554 1102 68-69 -0.18740636243315834 1102 70-71 -0.11282847256196504 1102 72-73 -0.05403788745704219 1102 74-75 -0.1665685195583393 1102 76-77 -0.11256902795847168 1102 78-79 -0.017084951729373188 1102 80-81 0.021254137620061897 1102 82-83 5.017099239807976E-4 1102 84-85 0.021671965931481907 1102 86-87 0.002452900275081049 1102 88-89 -0.06960372430297213 1102 90-91 -0.0587719812210139 1102 92-93 -0.012348497045216789 1102 94-95 -0.12526746579686687 1102 96-97 -0.09827209543473003 1102 98-99 -0.039022449343399046 1102 100-101 -0.08842247661242908 1102 102-103 -3.680177420406494E-5 1102 104-105 -0.10430826233791635 1102 106-107 -0.21167197988077646 1102 108-109 -0.22336486970626623 1102 110-111 -0.2026106031353052 1102 112-113 -0.2773339027318329 1102 114-115 -0.27382676641325787 1102 116-117 -0.4007247244489065 1102 118-119 -0.40502445902383855 1102 120-121 -0.34455265948525593 1102 122-123 -0.40366216335969796 1102 124-125 -0.47635640001973556 1103 1 0.1394769590340914 1103 2 0.13358698298718963 1103 3 0.13019854487586002 1103 4 0.07764910381298051 1103 5 0.04727178644876773 1103 6 0.07866578274650493 1103 7 0.053672294931388365 1103 8 0.1327068279327861 1103 9 0.20409774808614145 1103 10-11 0.23964029850401403 1103 12-13 0.2356413187779296 1103 14-15 0.2785830263931288 1103 16-17 0.28381534527957797 1103 18-19 0.2218594329517387 1103 20-21 0.20158065116893198 1103 22-23 0.2619365748928786 1103 24-25 0.2786499730328842 1103 26-27 0.2996927874900308 1103 28-29 0.29814164859238446 1103 30-31 0.36807090602125925 1103 32-33 0.40986303125739454 1103 34-35 0.3195929506176327 1103 36-37 0.3128948481091811 1103 38-39 0.37229319344415046 1103 40-41 0.3954950494549081 1103 42-43 0.38220999474906137 1103 44-45 0.46900636047578104 1103 46-47 0.44131558246372293 1103 48-49 0.4091033781896556 1103 50-51 0.2661989554453186 1103 52-53 0.40373880272175455 1103 54-55 0.3550758855421279 1103 56-57 0.25208403657413925 1103 58-59 0.28806979244189534 1103 60-61 0.277643680666138 1103 62-63 0.3212054268377855 1103 64-65 0.3128286348709324 1103 66-67 0.15929553270172647 1103 68-69 0.2195262048815394 1103 70-71 0.36444078362617205 1103 72-73 0.30958000066858915 1103 74-75 0.31379847975986763 1103 76-77 0.39642754045684114 1103 78-79 0.4753993466591453 1103 80-81 0.4173237062295527 1103 82-83 0.38263979354807276 1103 84-85 0.41057282265215633 1103 86-87 0.3973944257585771 1103 88-89 0.25320616973672827 1103 90-91 0.4145058918059945 1103 92-93 0.3318409271259952 1103 94-95 0.3010040534434282 1103 96-97 0.20738745061390063 1103 98-99 0.3245153307408728 1103 100-101 0.3154014633274329 1103 102-103 0.3754162657977993 1103 104-105 0.30716930436291534 1103 106-107 0.3311200814123296 1103 108-109 0.36473525489656566 1103 110-111 0.3546384344797744 1103 112-113 0.3610744201453713 1103 114-115 0.3034620814154252 1103 116-117 0.30529748463482065 1103 118-119 0.2769961618308976 1103 120-121 0.29563284287488756 1103 122-123 0.339625087532589 1103 124-125 0.07580629941228167 1104 1 0.11065308766626458 1104 2 0.0852111767924768 1104 3 0.09460397198017034 1104 4 0.11585794270663996 1104 5 0.15912120136384544 1104 6 0.13573317039669774 1104 7 0.20758492149496277 1104 8 0.18564252074077103 1104 9 0.21551630311679304 1104 10-11 0.19079838098753044 1104 12-13 0.15546135857324828 1104 14-15 0.19278717264131728 1104 16-17 0.20629072384848257 1104 18-19 0.18892957765242357 1104 20-21 0.15733160784928657 1104 22-23 0.1693232872031487 1104 24-25 0.2588741592380899 1104 26-27 0.16436454082052165 1104 28-29 0.16858873545726993 1104 30-31 0.21206647857009386 1104 32-33 0.20785580147866511 1104 34-35 0.2444340563808325 1104 36-37 0.2949841264168569 1104 38-39 0.27317464108161715 1104 40-41 0.2129105531340656 1104 42-43 0.2129874102203999 1104 44-45 0.17811578465649092 1104 46-47 0.19983458549486244 1104 48-49 0.20707011409251663 1104 50-51 0.13225853199146798 1104 52-53 0.15156804993377904 1104 54-55 0.11503348679501357 1104 56-57 0.16205121877960238 1104 58-59 0.15478887810369457 1104 60-61 0.21197106141157462 1104 62-63 0.09564376875893998 1104 64-65 0.07603235988060675 1104 66-67 0.017930800794573543 1104 68-69 0.0534989691035932 1104 70-71 0.08613966035043319 1104 72-73 0.20660448828975575 1104 74-75 0.19397472077621103 1104 76-77 0.20394814354748547 1104 78-79 0.255877812154381 1104 80-81 0.1868409960084989 1104 82-83 0.13887359789774223 1104 84-85 0.1883886727009454 1104 86-87 0.11298752714930771 1104 88-89 0.15285624262473618 1104 90-91 0.19262598763169336 1104 92-93 0.1913219063074365 1104 94-95 0.18388430608951012 1104 96-97 0.1141854800908888 1104 98-99 0.0826026653683769 1104 100-101 0.040931670009200616 1104 102-103 0.0972196032033743 1104 104-105 0.13891201091329464 1104 106-107 0.10735107211516492 1104 108-109 0.13468445563490405 1104 110-111 0.15572928859138102 1104 112-113 0.06271838224522241 1104 114-115 0.11262907216353568 1104 116-117 0.06650780554417679 1104 118-119 0.020818145306382263 1104 120-121 -0.1028312131910738 1104 122-123 -0.21172558422559007 1104 124-125 -0.31273544726409597 1105 1 0.07698146866302835 1105 2 0.10950469763520232 1105 3 0.10154562160435887 1105 4 0.06821822104847541 1105 5 0.023260994710653904 1105 6 0.04568691794290913 1105 7 0.09484370045786505 1105 8 0.15920751273593936 1105 9 0.10094336068156906 1105 10-11 0.1691701330532709 1105 12-13 0.16289557599147741 1105 14-15 0.17232139113769307 1105 16-17 0.22573991043577735 1105 18-19 0.2017476849659019 1105 20-21 0.22970463727638446 1105 22-23 0.14808679238701217 1105 24-25 0.2323653706603821 1105 26-27 0.18013523884589233 1105 28-29 0.19221766793364026 1105 30-31 0.22272925983538983 1105 32-33 0.187817818563893 1105 34-35 0.18885531979711345 1105 36-37 0.21715986321282799 1105 38-39 0.2905253858791994 1105 40-41 0.3229679153168661 1105 42-43 0.1673281779857092 1105 44-45 0.1266178298346503 1105 46-47 0.15794073715328238 1105 48-49 0.18028889255979408 1105 50-51 0.20789971091651438 1105 52-53 0.14362646033174542 1105 54-55 0.13980935718450382 1105 56-57 0.19836550181346269 1105 58-59 0.0949023859346525 1105 60-61 0.05076670499460789 1105 62-63 0.17815289719393945 1105 64-65 0.08611173554731266 1105 66-67 0.07092166568150304 1105 68-69 0.01005701606987941 1105 70-71 0.053774448284791276 1105 72-73 0.17731698012119068 1105 74-75 0.21479138240884765 1105 76-77 0.13426532920724554 1105 78-79 0.24744220650277526 1105 80-81 0.25961044515485554 1105 82-83 0.19398350198621728 1105 84-85 0.25775447999960477 1105 86-87 0.2512208613891431 1105 88-89 0.32673058199895877 1105 90-91 0.2889169569700343 1105 92-93 0.24773314843918826 1105 94-95 0.19131683387630005 1105 96-97 0.25522320022773926 1105 98-99 0.23348538515362094 1105 100-101 0.20400178182900675 1105 102-103 0.3321493378684792 1105 104-105 0.20780721562662308 1105 106-107 0.21541724387537897 1105 108-109 0.28262657304562566 1105 110-111 0.18945409075565323 1105 112-113 0.11468010066798229 1105 114-115 0.11003066038490772 1105 116-117 0.043680804332630885 1105 118-119 -0.034260862664336145 1105 120-121 0.007892346530880445 1105 122-123 -0.006444456105342056 1105 124-125 -0.18746158616821518 1106 1 0.04267725734289485 1106 2 0.11124191461360766 1106 3 0.09267283580720687 1106 4 0.07067228963060757 1106 5 0.07410737214733132 1106 6 0.06887576940287232 1106 7 0.06257256548276047 1106 8 0.09804732469051913 1106 9 0.1951402905332813 1106 10-11 0.16265781578552918 1106 12-13 0.1423979964472437 1106 14-15 0.11440683692021736 1106 16-17 0.23281540882069152 1106 18-19 0.17281037586840853 1106 20-21 0.16115387226859923 1106 22-23 0.08640910005014035 1106 24-25 0.19023652773341837 1106 26-27 0.13690956970119572 1106 28-29 0.1440630616129681 1106 30-31 0.135623553177453 1106 32-33 0.18142930296588133 1106 34-35 0.21654030178930128 1106 36-37 0.2908602951209076 1106 38-39 0.2551339995467785 1106 40-41 0.16821208415838385 1106 42-43 0.13055617007571385 1106 44-45 0.14428610858542612 1106 46-47 0.2718139788866907 1106 48-49 0.13569483224441825 1106 50-51 0.06697072914531788 1106 52-53 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-0.6561024848990655 2216 58-59 -0.6047215823350456 2216 60-61 -0.7084569826670517 2216 62-63 -0.6116172702547757 2216 64-65 -0.5398083037076944 2216 66-67 -0.5065121479651324 2216 68-69 -0.6927900701116769 2216 70-71 -0.7862747271466972 2216 72-73 -0.7259843842664324 2216 74-75 -0.7457769743900684 2216 76-77 -0.6468125458016942 2216 78-79 -0.6307269483223479 2216 80-81 -0.6287657334052312 2216 82-83 -0.5597797174013408 2216 84-85 -0.4989785131571267 2216 86-87 -0.5672183724103235 2216 88-89 -0.4791812937704947 2216 90-91 -0.5360649089229135 2216 92-93 -0.5236870596073402 2216 94-95 -0.5636176511715334 2216 96-97 -0.5068188037605026 2216 98-99 -0.5656789086013454 2216 100-101 -0.541302253103936 2216 102-103 -0.6470584808808049 2216 104-105 -0.6227290833338941 2216 106-107 -0.5327898168807863 2216 108-109 -0.47467758173556973 2216 110-111 -0.5116948053227368 2216 112-113 -0.4735091522695569 2216 114-115 -0.38025488591604883 2216 116-117 -0.3710820686872047 2216 118-119 -0.5365638628864637 2216 120-121 -0.4673121641556932 2216 122-123 -0.43479473238379995 2216 124-125 -0.4648013408997116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 246.0 3 10.0 4 42.0 5 103.0 6 253.0 7 508.0 8 912.0 9 1621.0 10 2614.0 11 3754.0 12 4564.0 13 6871.0 14 10410.0 15 8815.0 16 4107.0 17 3471.0 18 3713.0 19 3436.0 20 3424.0 21 3462.0 22 3673.0 23 4364.0 24 5583.0 25 7558.0 26 10326.0 27 13590.0 28 17498.0 29 22540.0 30 28704.0 31 36106.0 32 46281.0 33 59792.0 34 82538.0 35 125057.0 36 253429.0 37 562108.0 38 284190.0 39 1001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62856656198828 19.081925237863036 13.298316188583433 23.99119201156525 2 15.769785464081924 20.815418455080735 38.969639890967706 24.445156189869635 3 18.755017907706154 25.801297617100904 29.736997087308215 25.706687387884724 4 12.662709307458039 16.93787446040202 37.19104135186276 33.208374880277184 5 13.091886538869632 38.788132283154596 34.236769993018626 13.883211184957142 6 31.719336603505372 37.01526913745421 17.51640722126784 13.748987037772578 7 28.212167895964402 33.0037856386713 21.203633918043813 17.580412547320485 8 26.693424742757305 35.05385836375328 19.801939417486235 18.450777476003182 9 27.09006229889947 15.481590042012105 19.6725957382979 37.75575192079052 10-11 24.855656388434312 26.62607873489095 27.75814330345232 20.760121573222413 12-13 25.75506831731496 24.160034524434522 28.077813993461504 22.007083164789012 14-15 22.828360200015492 25.18230450600428 25.96515958329696 26.024175710683274 16-17 22.335145210410936 28.7112230231749 26.478753579389604 22.474878187024565 18-19 22.282491759258463 27.07776727236066 28.722011908962706 21.917729059418175 20-21 23.120393195915582 26.319597705742403 28.673732225958553 21.88627687238346 22-23 22.88500953479308 26.416448532404157 28.479185134821112 22.21935679798165 24-25 22.609969791119795 26.569736775776832 28.595035022383097 22.22525841072028 26-27 22.426214290544486 26.871307640763032 28.359009510226414 22.343468558466068 28-29 22.356818816829335 26.619541806689305 28.700724147438493 22.322915229042863 30-31 22.4535601193356 26.457683873771803 28.649242964013048 22.439513042879547 32-33 22.382849913381538 26.548589330130067 28.5732113502767 22.49534940621169 34-35 22.697961636408063 26.52998682894068 28.380021178202743 22.392030356448515 36-37 22.82298112590476 26.230486256004582 28.22609201351961 22.720440604571046 38-39 22.544222136703482 26.448415601405074 28.326819018438854 22.680543243452593 40-41 22.747182287292965 26.211367489736727 28.458713915633986 22.582736307336322 42-43 22.749395392069953 26.24308865820687 28.224862510865727 22.782653438857448 44-45 22.590516892931365 26.4087432527096 28.100633717379324 22.900106136979716 46-47 22.940275679085055 26.626416848120765 27.578144114924076 22.855163357870108 48-49 22.857297423903425 26.705689211648487 27.441088877160745 22.995924487287343 50-51 22.84996870915746 26.755237988681195 27.36691555898723 23.02787774317411 52-53 23.139024783084995 26.704367316376853 27.25235664921183 22.904251251326325 54-55 22.693914084813553 26.69508457134005 27.432755426102588 23.178245917743816 56-57 22.72936987468559 26.924754490543094 27.323821663725585 23.02205397104573 58-59 22.904518272731604 26.42670851077891 27.49579279172667 23.17298042476282 60-61 22.792643147920234 26.5633126244103 27.64091022540472 23.003134002264744 62-63 22.671844512176378 26.629521342321816 27.717815616405005 22.980818529096794 64-65 22.450841410141184 26.500454301230608 27.707856644908567 23.340847643719638 66-67 22.466609781677214 26.80128286306906 27.3662393325276 23.365868022726126 68-69 22.47124113796723 26.557865827325415 27.65547459614643 23.31541843856093 70-71 22.478672726479136 26.500993131379204 27.52569201062537 23.49464213151629 72-73 22.513017391357803 26.595222140938233 27.25525152612392 23.636508941580036 74-75 22.460769644071277 26.779366978263624 27.12371993404948 23.636143443615623 76-77 22.63241517120676 26.64041062942256 27.214834445879887 23.512339753490792 78-79 22.573914625794718 26.77358831579038 27.16192672901884 23.490570329396057 80-81 22.638051160330257 26.989762216748876 26.918666072815604 23.45352055010526 82-83 22.55507249762399 26.79618042705545 27.081179142225174 23.567567933095386 84-85 22.664344545987703 26.631088958205517 26.930780230089123 23.773786265717657 86-87 22.573453562299512 26.536355778723948 27.12743917957747 23.762751479399068 88-89 22.493351464399137 26.59352765212944 27.04958092402042 23.863539959451003 90-91 22.549300766257026 26.716055803454104 27.073103483889057 23.661539946399817 92-93 22.50647640522932 26.38620276711867 27.0653800331228 24.041940794529204 94-95 22.38764497373168 26.266234045666188 27.116865456754084 24.229255523848046 96-97 22.490216232631738 26.291254424672676 27.039929328187455 24.17860001450813 98-99 22.402795900207018 26.218111568590384 26.89965835058486 24.479434180617734 100-101 22.024910953270293 26.390321601009177 27.144006727838523 24.44076071788201 102-103 22.255811551669236 26.13000515161612 27.065287820423762 24.548895476290884 104-105 22.299104816922874 26.263232329255732 26.94383335572085 24.493829498100546 106-107 22.17380372465534 26.297494150641125 26.96778825997096 24.560913864732576 108-109 22.18870406772211 26.25196915742347 26.65709688177678 24.902229893077635 110-111 22.018499215699897 26.4459601397877 26.828319888701007 24.7072207558114 112-113 21.887022230637484 26.649131909651224 26.901641017192134 24.562204842519154 114-115 21.9812636090575 26.595218218278525 26.62998240581702 24.79353576684695 116-117 21.806786553531825 26.563091218422883 26.816830062597113 24.81329216544818 118-119 21.750516698490294 26.699018979832466 26.529562776561256 25.020901545115986 120-121 21.68653293526001 26.829867302403652 26.631732279809935 24.8518674825264 122-123 21.698086239558016 26.96612981210346 26.484623615227832 24.851160333110688 124-125 21.619471651484858 26.986163471749848 26.405222932089895 24.989141944675403 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 52.0 1 47.5 2 109.5 3 162.0 4 105.0 5 88.5 6 221.0 7 556.5 8 992.0 9 1179.0 10 921.5 11 627.0 12 605.5 13 733.5 14 890.5 15 998.5 16 1103.0 17 1207.0 18 1309.0 19 1431.5 20 1573.0 21 1826.5 22 2199.0 23 2676.0 24 3364.0 25 4322.0 26 5749.0 27 7647.0 28 9640.0 29 12290.5 30 15898.0 31 19014.0 32 22753.5 33 26476.0 34 29832.0 35 33085.5 36 37974.0 37 43980.5 38 47564.5 39 49475.5 40 50177.0 41 52094.5 42 53864.0 43 56190.5 44 59976.0 45 62634.5 46 62685.0 47 62404.0 48 63277.0 49 63424.0 50 62047.0 51 59455.0 52 58950.5 53 58764.5 54 57441.5 55 53184.5 56 47061.0 57 42451.5 58 40264.5 59 37167.0 60 30814.0 61 24777.5 62 19387.0 63 14445.0 64 11110.0 65 8633.0 66 6071.5 67 4863.0 68 3779.0 69 2376.5 70 1587.5 71 1191.0 72 977.5 73 731.5 74 622.0 75 540.5 76 328.5 77 141.0 78 63.5 79 29.5 80 15.0 81 10.0 82 4.5 83 2.0 84 2.0 85 2.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026003981129593266 2 0.0 3 0.0 4 0.0 5 0.05649564694585393 6 0.015737633969682925 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.459005307762957E-4 22-23 0.0 24-25 0.0 26-27 2.459005307762957E-4 28-29 1.536878317351848E-4 30-31 6.147513269407393E-5 32-33 0.0 34-35 9.221269904111088E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.536878317351848E-4 46-47 0.0 48-49 4.610634952055544E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.3811322981740655E-4 58-59 9.221269904111088E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.221269904111088E-5 70-71 6.147513269407393E-5 72-73 2.459005307762957E-4 74-75 0.0 76-77 3.073756634703696E-5 78-79 0.0 80-81 2.151629644292587E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.073756634703696E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.9958836251148045E-4 100-101 0.0 102-103 0.0 104-105 0.0014446656183107372 106-107 0.0 108-109 0.0016290910163929587 110-111 0.0039651460587677686 112-113 0.0 114-115 0.0 116-117 9.221269904111088E-5 118-119 0.0 120-121 3.3811322981740655E-4 122-123 4.610634952055544E-4 124-125 9.221269904111088E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1626674.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.32139639851779 #Duplication Level Percentage of deduplicated Percentage of total 1 81.93463746516179 51.88215657700357 2 10.691815007208854 13.540413125821866 3 3.2565808363626143 6.1863373812940115 4 1.4158234506556429 3.58607671797133 5 0.7144633129199692 2.2620407324801817 6 0.43159657616388136 1.6397578730109725 7 0.2741457724427769 1.215150518947885 8 0.18950407482465675 0.9599730112885172 9 0.15018712331000178 0.8559052532159125 >10 0.8325842767794855 9.920649508632925 >50 0.07723493461198408 3.432390546176642 >100 0.030605544212159337 3.421520424938038 >500 2.0540633654273386E-4 0.10184180374189014 >1k 6.162190096282015E-4 0.9957865254762592 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3256 0.2001630320519047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2749 0.16899513977600922 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1651 0.10149544407791604 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 6.147513269407393E-5 0.0 16-17 0.0 0.0 0.0 6.147513269407393E-5 0.0 18-19 0.0 0.0 0.0 1.2295026538814785E-4 0.0 20-21 0.0 0.0 0.0 1.2295026538814785E-4 0.0 22-23 0.0 0.0 0.0 2.1516296442925873E-4 0.0 24-25 0.0 0.0 0.0 4.3032592885851746E-4 0.0 26-27 0.0 0.0 0.0 0.0010143396894522197 0.0 28-29 0.0 0.0 0.0 0.00313523176739777 0.0 30-31 0.0 0.0 0.0 0.011219211716668492 0.0 32-33 0.0 0.0 0.0 0.03144453037301881 0.0 34-35 0.0 0.0 0.0 0.06006120464211022 0.0 36-37 0.0 0.0 0.0 0.10967163672622787 0.0 38-39 0.0 0.0 0.0 0.1852860499399388 0.0 40-41 0.0 0.0 0.0 0.27175082407415374 0.0 42-43 0.0 0.0 0.0 0.3557873304669528 0.0 44-45 0.0 0.0 0.0 0.4572212994121748 0.0 46-47 0.0 0.0 0.0 0.5806941034282223 0.0 48-49 0.0 0.0 0.0 0.7133574397820337 0.0 50-51 0.0 0.0 0.0 0.85831580267466 0.0 52-53 0.0 0.0 0.0 1.0030590026028572 0.0 54-55 0.0 0.0 0.0 1.15739232323133 0.0 56-57 0.0 0.0 0.0 1.3424324726404921 0.0 58-59 0.0 0.0 0.0 1.551109810570526 0.0 60-61 0.0 0.0 0.0 1.772973564463439 0.0 62-63 0.0 0.0 0.0 1.9994172157420602 0.0 64-65 0.0 0.0 0.0 2.228965361221732 0.0 66-67 0.0 0.0 0.0 2.4891588603494004 0.0 68-69 0.0 0.0 0.0 2.780981315248169 0.0 70-71 0.0 0.0 0.0 3.109104836002788 0.0 72-73 0.0 0.0 0.0 3.444420947282615 0.0 74-75 0.0 0.0 0.0 3.7784460807758657 0.0 76-77 0.0 0.0 0.0 4.130544903281174 0.0 78-79 0.0 0.0 0.0 4.525553368406946 0.0 80-81 0.0 0.0 0.0 4.964024752347428 0.0 82-83 0.0 0.0 0.0 5.4251189851193296 0.0 84-85 0.0 0.0 0.0 5.886059530059496 0.0 86-87 0.0 0.0 0.0 6.3551147925152796 0.0 88-89 0.0 0.0 0.0 6.859118667907644 0.0 90-91 0.0 0.0 0.0 7.418450162724676 0.0 92-93 0.0 0.0 0.0 8.001172945531803 0.0 94-95 0.0 0.0 0.0 8.583834253206236 0.0 96-97 0.0 0.0 0.0 9.158934119559298 0.0 98-99 0.0 0.0 0.0 9.762343284517979 0.0 100-101 0.0 0.0 0.0 10.432053380087222 0.0 102-103 0.0 0.0 0.0 11.139078881201765 0.0 104-105 0.0 0.0 0.0 11.845397418290327 0.0 106-107 0.0 0.0 0.0 12.537576674859253 0.0 108-109 0.0 0.0 0.0 13.242512021462197 0.0 110-111 0.0 0.0 0.0 13.987344729183597 0.0 112-113 0.0 0.0 0.0 14.789380047876833 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2765 0.0 76.67468 1 GTATCAA 5095 0.0 56.337807 1 ATCAACG 6305 0.0 44.762077 3 TCAACGC 6460 0.0 43.68806 4 CAACGCA 6465 0.0 43.65427 5 TATCAAC 6630 0.0 42.747463 2 AACGCAG 6675 0.0 42.254883 6 ACGCAGA 8255 0.0 34.235207 7 CGCAGAG 8220 0.0 34.019073 8 GTACATG 8870 0.0 32.830864 1 TACATGG 9065 0.0 31.658926 2 TGGTATC 1295 0.0 30.805054 2 ACATGGG 9025 0.0 30.347824 3 GCAGAGT 9330 0.0 29.46163 9 GAGTACT 4600 0.0 27.549786 12-13 CAGAGTA 9055 0.0 26.315432 10-11 TATCACG 160 8.697494E-6 26.049238 2 CATGGGG 3355 0.0 25.910587 4 CATGGGA 6525 0.0 25.73271 4 GTACTTT 4940 0.0 25.171886 14-15 >>END_MODULE