FastQCFastQC Report
Thu 26 May 2016
SRR1364356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158333
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG53280.246857180981804No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25850.054.5821151
GGTATCA15550.050.536611
ATCAACG29600.046.649623
TCAACGC29950.046.104474
CAACGCA31150.044.5194445
AACGCAG33250.041.886696
TATCAAC33950.040.32182
GTACATG77450.035.9738541
ACGCAGA39250.035.6228877
TACATGG79250.034.47192
ACATGGG77650.034.185763
CGCAGAG40400.034.019788
TAAGGTA8950.033.250554
GTGTAAG9600.031.6272581
TAAGGTG17700.030.936165
AGGTAAG15400.030.1457232
CATGGGG43200.028.2437234
CATGGGA39400.027.3422474
GGTAAGG18250.026.4164223
TGTAAGG11800.025.724092
Produced by FastQC (version 0.11.5)