##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364356_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2158333 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.705037174523117 31.0 31.0 34.0 27.0 34.0 2 30.767052628116236 31.0 31.0 34.0 27.0 34.0 3 30.91874747779884 33.0 31.0 34.0 27.0 34.0 4 34.35676932150878 37.0 35.0 37.0 30.0 37.0 5 34.42986925557826 37.0 35.0 37.0 30.0 37.0 6 34.4974857911175 37.0 35.0 37.0 31.0 37.0 7 34.41245535327496 37.0 35.0 37.0 30.0 37.0 8 34.33583232985828 37.0 35.0 37.0 30.0 37.0 9 35.85103827815263 39.0 37.0 39.0 30.0 39.0 10-11 35.74495038532052 39.0 37.0 39.0 30.0 39.0 12-13 35.749475636984656 39.0 37.0 39.0 30.0 39.0 14-15 36.98897412957129 40.0 37.0 41.0 30.0 41.0 16-17 36.973649802880274 40.0 37.0 41.0 30.0 41.0 18-19 36.95793119041409 40.0 37.0 41.0 30.0 41.0 20-21 36.96606316078196 40.0 37.0 41.0 30.0 41.0 22-23 36.91968848180517 40.0 37.0 41.0 30.0 41.0 24-25 36.90599828664066 40.0 37.0 41.0 30.0 41.0 26-27 36.83109696233157 40.0 37.0 41.0 30.0 41.0 28-29 36.81293224910151 40.0 37.0 41.0 30.0 41.0 30-31 36.69651578324568 40.0 37.0 41.0 30.0 41.0 32-33 36.638101951830414 40.0 36.5 41.0 30.0 41.0 34-35 36.61803901436896 40.0 36.0 41.0 30.0 41.0 36-37 36.553043251435255 40.0 36.0 41.0 30.0 41.0 38-39 36.446346787080586 40.0 36.0 41.0 29.0 41.0 40-41 36.37455364857971 40.0 36.0 41.0 29.0 41.0 42-43 36.21977493741698 40.0 36.0 41.0 29.0 41.0 44-45 36.08597491675288 40.0 35.0 41.0 28.0 41.0 46-47 35.95544014755832 40.0 35.0 41.0 28.0 41.0 48-49 35.78142761103129 39.0 35.0 41.0 28.0 41.0 50-51 35.634319866304224 39.0 35.0 41.0 27.5 41.0 52-53 35.44009126487896 39.0 35.0 41.0 27.0 41.0 54-55 35.234776793015726 39.0 34.5 41.0 27.0 41.0 56-57 35.01903181761109 38.5 34.0 40.0 26.0 41.0 58-59 34.771193555396685 38.0 34.0 40.0 26.0 41.0 60-61 34.50157135159403 37.5 34.0 40.0 26.0 41.0 62-63 34.22285764059578 37.0 34.0 40.0 26.0 41.0 64-65 33.85668963037678 36.5 33.0 40.0 25.0 41.0 66-67 33.5370383068785 36.0 33.0 39.0 24.5 41.0 68-69 33.204955166788444 35.5 33.0 39.0 24.0 41.0 70-71 32.82805271475718 35.0 32.5 38.5 23.0 40.0 72-73 32.49385891797049 35.0 32.0 37.5 23.0 40.0 74-75 32.16015253438649 35.0 32.0 37.0 22.0 39.0 76-77 31.94999196138872 35.0 32.0 36.5 22.5 39.0 78-79 31.795815335261054 35.0 32.0 36.0 22.0 39.0 80-81 31.567231979495286 35.0 32.0 36.0 21.5 37.5 82-83 31.288388770407533 35.0 32.0 35.5 20.0 37.0 84-85 31.067478234359573 35.0 32.0 35.0 20.0 37.0 86-87 30.887219210381346 35.0 32.0 35.0 20.0 36.0 88-89 30.636238476639146 35.0 31.5 35.0 18.0 36.0 90-91 30.518892358130095 35.0 31.0 35.0 18.0 36.0 92-93 30.384978128954153 35.0 31.0 35.0 17.5 35.5 94-95 30.279614174457787 35.0 31.0 35.0 16.5 35.0 96-97 30.175360335962985 35.0 31.0 35.0 14.0 35.0 98-99 30.036204561575992 34.0 31.0 35.0 10.0 35.0 100-101 29.93442323311556 34.0 31.0 35.0 8.5 35.0 102-103 28.911344078972057 33.5 29.0 34.5 7.0 35.0 104-105 29.627880637510522 34.0 30.5 35.0 7.0 35.0 106-107 29.994467489493047 34.5 31.0 35.0 7.0 35.0 108-109 30.05001545173984 35.0 31.0 35.0 2.0 35.0 110-111 29.969338836963527 35.0 31.0 35.0 2.0 35.0 112-113 29.87081164027979 35.0 31.0 35.0 2.0 35.0 114-115 29.765321430937675 35.0 31.0 35.0 2.0 35.0 116-117 29.626988977141153 35.0 31.0 35.0 2.0 35.0 118-119 29.432397827397345 35.0 31.0 35.0 2.0 35.0 120-121 29.23030134830909 35.0 31.0 35.0 2.0 35.0 122-123 29.043474292428463 35.0 31.0 35.0 2.0 35.0 124-125 28.18290991241852 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36954401465917286 1101 2 0.2639531330980951 1101 3 0.3940895210275528 1101 4 0.4460989512952551 1101 5 0.38172497339661504 1101 6 0.3980760996321706 1101 7 0.40503009041506743 1101 8 0.2038498860949005 1101 9 0.40628877989737333 1101 10-11 0.5478887746960766 1101 12-13 0.6282929263463117 1101 14-15 0.6389975530706593 1101 16-17 0.624493519141069 1101 18-19 0.503001789603637 1101 20-21 0.6296091376052146 1101 22-23 0.76581780285305 1101 24-25 0.7106818542365474 1101 26-27 0.9293701910436667 1101 28-29 0.7839299285361463 1101 30-31 0.8696571120429439 1101 32-33 0.6803278502535335 1101 34-35 0.7401869217632893 1101 36-37 0.711539239944635 1101 38-39 0.788167970178641 1101 40-41 0.7095656013023017 1101 42-43 0.8283227104507063 1101 44-45 0.8088427762119821 1101 46-47 0.8285392695943017 1101 48-49 0.6892441788613866 1101 50-51 0.7278211753384554 1101 52-53 0.7318771017778047 1101 54-55 0.7575550970492273 1101 56-57 0.684658395070521 1101 58-59 0.7617528303767003 1101 60-61 0.6428381083972141 1101 62-63 0.5898419518350764 1101 64-65 0.7201732986307405 1101 66-67 0.6676574609736861 1101 68-69 0.6113654045777537 1101 70-71 0.679330997085458 1101 72-73 0.6027193374061852 1101 74-75 0.6404327354895827 1101 76-77 0.7281729447174321 1101 78-79 0.6731357360038679 1101 80-81 0.6567039994931179 1101 82-83 0.565695113486786 1101 84-85 0.5650343243286855 1101 86-87 0.585899929603297 1101 88-89 0.6429146642905152 1101 90-91 0.53885627308442 1101 92-93 0.4914191071394711 1101 94-95 0.5924505127125208 1101 96-97 0.5171056230859215 1101 98-99 0.4809786232718949 1101 100-101 0.3869510806352281 1101 102-103 0.18083494703518 1101 104-105 0.38316843863330874 1101 106-107 0.431555280907709 1101 108-109 0.497693964480181 1101 110-111 0.47057506470130406 1101 112-113 0.5107791953423124 1101 114-115 0.3915531346943517 1101 116-117 0.3685980930113537 1101 118-119 0.5138787898980759 1101 120-121 0.4619433410136402 1101 122-123 0.3777761184688444 1101 124-125 0.33202070211873647 1102 1 0.3436336908265254 1102 2 0.3072320089458991 1102 3 0.4479112572835291 1102 4 0.5215837741043714 1102 5 0.5523459703832501 1102 6 0.5169473948412175 1102 7 0.45876166038349453 1102 8 0.4952237046880512 1102 9 0.6567095794603546 1102 10-11 0.6548539272631828 1102 12-13 0.545143498007171 1102 14-15 0.6295985419979218 1102 16-17 0.6803105496024813 1102 18-19 0.7440829823198953 1102 20-21 0.6956069029510701 1102 22-23 0.6625388214814834 1102 24-25 0.6544215768579065 1102 26-27 0.602763730709249 1102 28-29 0.56904072330628 1102 30-31 0.6750181997592932 1102 32-33 0.6946672578299342 1102 34-35 0.5506494239408966 1102 36-37 0.4699538414939681 1102 38-39 0.5463520682561978 1102 40-41 0.5401959559855811 1102 42-43 0.3669134104341225 1102 44-45 0.38737733001558183 1102 46-47 0.5194105595271523 1102 48-49 0.44831406414104435 1102 50-51 0.43437405032869947 1102 52-53 0.47066329395561723 1102 54-55 0.48645536135799006 1102 56-57 0.4460869249087196 1102 58-59 0.49426473972274465 1102 60-61 0.5069191193676303 1102 62-63 0.48499650787540105 1102 64-65 0.39102701320383915 1102 66-67 0.403170061029293 1102 68-69 0.4270579754126942 1102 70-71 0.2656763822062089 1102 72-73 0.4063853554471706 1102 74-75 0.3588258007693099 1102 76-77 0.38922505621497194 1102 78-79 0.5295169040445238 1102 80-81 0.41104693834689954 1102 82-83 0.3168648491205488 1102 84-85 0.2914135599654166 1102 86-87 0.29794734218415897 1102 88-89 0.35632074244047374 1102 90-91 0.3081116041308398 1102 92-93 0.23232708071293118 1102 94-95 0.20555780786239097 1102 96-97 0.2622524485683684 1102 98-99 0.18861844013917306 1102 100-101 0.12582682668588419 1102 102-103 -0.18176369059649744 1102 104-105 -0.01813754042794713 1102 106-107 0.0977505923926607 1102 108-109 0.11064217313420599 1102 110-111 0.19462943280569078 1102 112-113 0.18655125948983553 1102 114-115 0.18821746256497818 1102 116-117 0.24215457267452223 1102 118-119 0.15835914801558104 1102 120-121 0.13218704343549348 1102 122-123 0.2340843620512274 1102 124-125 0.21048135160054215 1103 1 0.48445493854146093 1103 2 0.4231020621448245 1103 3 0.5398656763659275 1103 4 0.5151146858455959 1103 5 0.55116065144977 1103 6 0.4865433636851577 1103 7 0.3457266720412875 1103 8 0.4278136099281298 1103 9 0.5805064137626559 1103 10-11 0.6632695993043711 1103 12-13 0.5904803304487984 1103 14-15 0.701385632479699 1103 16-17 0.6038285614152059 1103 18-19 0.6186063025691411 1103 20-21 0.6538759622189616 1103 22-23 0.7488089295210401 1103 24-25 0.7785407292190669 1103 26-27 0.7450369248278079 1103 28-29 0.6817915769345433 1103 30-31 0.7768489678117732 1103 32-33 0.7862293575942445 1103 34-35 0.7862741059580713 1103 36-37 0.6575750468219681 1103 38-39 0.704116493924829 1103 40-41 0.6475377179866513 1103 42-43 0.5931333766188089 1103 44-45 0.6171569484611439 1103 46-47 0.6236124735724076 1103 48-49 0.630854981433771 1103 50-51 0.5762149217700028 1103 52-53 0.5713535967256078 1103 54-55 0.5724460925889048 1103 56-57 0.43780350415773484 1103 58-59 0.43973258547533334 1103 60-61 0.342195125454424 1103 62-63 0.40706067936102386 1103 64-65 0.4777633497701146 1103 66-67 0.42774057692757594 1103 68-69 0.48513952701827634 1103 70-71 0.5292860007875788 1103 72-73 0.462163157019603 1103 74-75 0.4801340473463256 1103 76-77 0.5005176811568681 1103 78-79 0.5603740448427708 1103 80-81 0.5160221736807458 1103 82-83 0.5031333311575175 1103 84-85 0.31384814592741606 1103 86-87 0.4110305100917486 1103 88-89 0.5433869819981396 1103 90-91 0.4497838701899468 1103 92-93 0.4024808608317407 1103 94-95 0.3867223509173243 1103 96-97 0.4655073916393384 1103 98-99 0.3661623096428599 1103 100-101 0.3909711699845495 1103 102-103 0.19923264433823462 1103 104-105 0.3302822945494164 1103 106-107 0.32306064383254807 1103 108-109 0.4234386934147558 1103 110-111 0.3791160100157285 1103 112-113 0.355438281965899 1103 114-115 0.30127637901348336 1103 116-117 0.22221825654414218 1103 118-119 0.20129328447101003 1103 120-121 0.0820037994106606 1103 122-123 0.056979928986134354 1103 124-125 0.09252631962603175 1104 1 0.4849125101290319 1104 2 0.4838375036460185 1104 3 0.5124362829668776 1104 4 0.6040337173721682 1104 5 0.5636464599100108 1104 6 0.5161628386203887 1104 7 0.5943899139094313 1104 8 0.596246553581004 1104 9 0.7915648332400806 1104 10-11 0.8246049191709872 1104 12-13 0.723006309629568 1104 14-15 0.8392682570363519 1104 16-17 0.8374477411902106 1104 18-19 0.8803586735782218 1104 20-21 0.8298848833085515 1104 22-23 0.8259205908125935 1104 24-25 0.8146934621839321 1104 26-27 0.8656603665736284 1104 28-29 0.8509046982804591 1104 30-31 0.8927280568543097 1104 32-33 0.822062486234941 1104 34-35 0.7122594297934199 1104 36-37 0.7227977202173861 1104 38-39 0.6200907684774819 1104 40-41 0.7104950549749418 1104 42-43 0.7047824919025842 1104 44-45 0.5884088095772668 1104 46-47 0.5411725476957088 1104 48-49 0.5377129797951952 1104 50-51 0.5716697839075664 1104 52-53 0.5028809108106032 1104 54-55 0.5210762204828185 1104 56-57 0.49480970975495353 1104 58-59 0.681369979492537 1104 60-61 0.5079198550381321 1104 62-63 0.6127585167722316 1104 64-65 0.6895237818392843 1104 66-67 0.6803945241783111 1104 68-69 0.5696002567469947 1104 70-71 0.39420588776611254 1104 72-73 0.4952854166139389 1104 74-75 0.4951960991987647 1104 76-77 0.5310499189428519 1104 78-79 0.6404794675787677 1104 80-81 0.6249200917300186 1104 82-83 0.5795752973384154 1104 84-85 0.503769742619717 1104 86-87 0.5810086504743097 1104 88-89 0.5871153261959776 1104 90-91 0.5047151195553852 1104 92-93 0.5002143251073257 1104 94-95 0.5118207567316873 1104 96-97 0.4285450737307208 1104 98-99 0.39023430127217296 1104 100-101 0.36718998990943774 1104 102-103 0.18154793276809045 1104 104-105 0.42178786894870157 1104 106-107 0.3977897835164832 1104 108-109 0.38157817145172856 1104 110-111 0.4074939169067804 1104 112-113 0.3029892531794509 1104 114-115 0.285448412014663 1104 116-117 0.28443631970898053 1104 118-119 0.31510034691177324 1104 120-121 0.3012352457662537 1104 122-123 0.236941012513455 1104 124-125 0.1267823460351849 1105 1 0.27138713439865114 1105 2 0.19312067668014166 1105 3 0.17268052497886544 1105 4 0.328014952887024 1105 5 0.31573583619255174 1105 6 0.35439120401935753 1105 7 0.3433057142486291 1105 8 0.48614801071504843 1105 9 0.45452442005139915 1105 10-11 0.45396859723251026 1105 12-13 0.4906673016385099 1105 14-15 0.6428466127351129 1105 16-17 0.5274335192710637 1105 18-19 0.518066043890208 1105 20-21 0.5338511224918392 1105 22-23 0.550626155914884 1105 24-25 0.5595619551034048 1105 26-27 0.5396806836264503 1105 28-29 0.5998172459092714 1105 30-31 0.6338593192240722 1105 32-33 0.6808404509668264 1105 34-35 0.47263974203757186 1105 36-37 0.483960955393421 1105 38-39 0.4670709744426702 1105 40-41 0.5205751331398005 1105 42-43 0.5507968211166201 1105 44-45 0.504766788088169 1105 46-47 0.5839067011813981 1105 48-49 0.45488097230522584 1105 50-51 0.43387503599217325 1105 52-53 0.5035248583498202 1105 54-55 0.49966246957395555 1105 56-57 0.4394546835465789 1105 58-59 0.45770247457715385 1105 60-61 0.47854850414473304 1105 62-63 0.4680639235521866 1105 64-65 0.40284022662505237 1105 66-67 0.39077613305084924 1105 68-69 0.3696239792539089 1105 70-71 0.4369928420983271 1105 72-73 0.2626729136051864 1105 74-75 0.14274211636184475 1105 76-77 0.2128389559899766 1105 78-79 0.44358756156088575 1105 80-81 0.45994885950263154 1105 82-83 0.46144474316835726 1105 84-85 0.35852365903896555 1105 86-87 0.37350925489919007 1105 88-89 0.3835587057317582 1105 90-91 0.3159237099902228 1105 92-93 0.42123112058875023 1105 94-95 0.34469391323580467 1105 96-97 0.34815148508130633 1105 98-99 0.3632951757023122 1105 100-101 0.1840370423879527 1105 102-103 0.09489456983538958 1105 104-105 0.1710567443156208 1105 106-107 0.10045115114663261 1105 108-109 0.12087659836078757 1105 110-111 0.11370385788478998 1105 112-113 0.16817095804704607 1105 114-115 0.10274545246706523 1105 116-117 0.01546092080415562 1105 118-119 0.007343416878644149 1105 120-121 -0.13711675827316938 1105 122-123 -0.2742746002111822 1105 124-125 -0.2680331989974327 1106 1 0.3219499667817658 1106 2 0.4489917741829892 1106 3 0.44312832211768693 1106 4 0.5039593083494864 1106 5 0.4550861097523935 1106 6 0.39488115333619334 1106 7 0.33645164467775146 1106 8 0.4221429197671327 1106 9 0.5728162263168883 1106 10-11 0.6262268614279662 1106 12-13 0.47139108938115726 1106 14-15 0.5715579229216914 1106 16-17 0.6479488576779531 1106 18-19 0.5268471861677568 1106 20-21 0.508085065956891 1106 22-23 0.5901302825922059 1106 24-25 0.5934333525209254 1106 26-27 0.7051531623953053 1106 28-29 0.5536236053787746 1106 30-31 0.6747686912639068 1106 32-33 0.6871613772885397 1106 34-35 0.7296446759449182 1106 36-37 0.6965243136637937 1106 38-39 0.5679507558228423 1106 40-41 0.5972443198268422 1106 42-43 0.6227441657056261 1106 44-45 0.5671541797167805 1106 46-47 0.5052610318819006 1106 48-49 0.4498228059621141 1106 50-51 0.44097684613961974 1106 52-53 0.4085347895681224 1106 54-55 0.4357593513424476 1106 56-57 0.3534541510943754 1106 58-59 0.3988750148319724 1106 60-61 0.309355554489116 1106 62-63 0.3195346802303405 1106 64-65 0.42434437645246703 1106 66-67 0.31177349445822244 1106 68-69 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0.6872414404190899 2203 80-81 0.7150570909687914 2203 82-83 0.7218199150126381 2203 84-85 0.7287006100696871 2203 86-87 0.6923223716252878 2203 88-89 0.6416094814290361 2203 90-91 0.5825646415121639 2203 92-93 0.6300735463425937 2203 94-95 0.6100647308210796 2203 96-97 0.6214200352560297 2203 98-99 0.6622084390576539 2203 100-101 0.6136104330096508 2203 102-103 0.6607185101064843 2203 104-105 0.7485101016896714 2203 106-107 0.7411164688207457 2203 108-109 0.7932330160392631 2203 110-111 0.8642782066470822 2203 112-113 0.859184691690686 2203 114-115 0.8411453160628284 2203 116-117 1.0366631566367026 2203 118-119 0.9923348787773953 2203 120-121 0.9512856770665046 2203 122-123 1.0039927827193615 2203 124-125 1.152522488907767 2204 1 0.19720344252012012 2204 2 0.21393745808163445 2204 3 0.24172426858935836 2204 4 0.24996880545003108 2204 5 0.23349775399226047 2204 6 0.2525536057456179 2204 7 0.16519472472273833 2204 8 0.22982663377357682 2204 9 0.33376970101349457 2204 10-11 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9 0.354974372187435 2205 10-11 0.42147333593742786 2205 12-13 0.42218159242430175 2205 14-15 0.4204184899817207 2205 16-17 0.32680825367557276 2205 18-19 0.3814741578901888 2205 20-21 0.47097873241317245 2205 22-23 0.4766964546227115 2205 24-25 0.47618432179059056 2205 26-27 0.4291985938689393 2205 28-29 0.4561528666718999 2205 30-31 0.2718043228131961 2205 32-33 0.4495606291017964 2205 34-35 0.5650881368007177 2205 36-37 0.547365808212426 2205 38-39 0.5381773923196818 2205 40-41 0.5900975163781652 2205 42-43 0.4609721327592524 2205 44-45 0.5350713805759 2205 46-47 0.4512627518348893 2205 48-49 0.5337966014651627 2205 50-51 0.40911254353422066 2205 52-53 0.37919051626084155 2205 54-55 0.5217438897265296 2205 56-57 0.5336023443714026 2205 58-59 0.5046086584848695 2205 60-61 0.6294294499635242 2205 62-63 0.5562272264181516 2205 64-65 0.569599490241302 2205 66-67 0.5983885924700374 2205 68-69 0.4825453688040682 2205 70-71 0.5950815429510143 2205 72-73 0.6008476623988628 2205 74-75 0.717054347825389 2205 76-77 0.6559962987978771 2205 78-79 0.5540654272260319 2205 80-81 0.6021935349206444 2205 82-83 0.5470118643594759 2205 84-85 0.5795745914187833 2205 86-87 0.6157544117728939 2205 88-89 0.6545536257383517 2205 90-91 0.5858782769244684 2205 92-93 0.6116324929373143 2205 94-95 0.5663716918392225 2205 96-97 0.5853157855597999 2205 98-99 0.6467973606935757 2205 100-101 0.5949688753479023 2205 102-103 0.6473957684783755 2205 104-105 0.6126535348048172 2205 106-107 0.5813237341709403 2205 108-109 0.6703489410932839 2205 110-111 0.6604934023386839 2205 112-113 0.6284543610339952 2205 114-115 0.6330033982573333 2205 116-117 0.6746458417573074 2205 118-119 0.748063630986529 2205 120-121 0.7801261412092408 2205 122-123 0.9018400287563537 2205 124-125 0.9155651136184701 2206 1 0.2625377008334304 2206 2 0.32501651098681705 2206 3 0.3249674871513939 2206 4 0.3161849828161962 2206 5 0.24349302449346766 2206 6 0.2104115543006202 2206 7 0.1909326308193613 2206 8 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0.4328899509200852 2206 74-75 0.4449111520558944 2206 76-77 0.44085117346051916 2206 78-79 0.3607713097116054 2206 80-81 0.38384143547978766 2206 82-83 0.31324140157322944 2206 84-85 0.28794706321794905 2206 86-87 0.30206810287329944 2206 88-89 0.4454297799512048 2206 90-91 0.4116768566467712 2206 92-93 0.44382904917823396 2206 94-95 0.3725710839003895 2206 96-97 0.3997182367817942 2206 98-99 0.43979385049227204 2206 100-101 0.5330455589940399 2206 102-103 0.5029435908138211 2206 104-105 0.48896812219879493 2206 106-107 0.5457717892608649 2206 108-109 0.4367084596652582 2206 110-111 0.4199820884643799 2206 112-113 0.5053977162197292 2206 114-115 0.51722754155006 2206 116-117 0.5893676891668029 2206 118-119 0.6180496717308692 2206 120-121 0.6214897419568643 2206 122-123 0.5734443115739936 2206 124-125 0.4908143142354646 2207 1 0.23384649000018953 2207 2 0.28712008548889045 2207 3 0.1954413881180912 2207 4 0.1335130315154629 2207 5 0.1649820673483262 2207 6 0.1649144082759335 2207 7 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32-33 -2.012555153227744 2216 34-35 -1.6547751634921752 2216 36-37 -1.608740432365444 2216 38-39 -1.875849943617645 2216 40-41 -1.797175576722978 2216 42-43 -1.5631620323574964 2216 44-45 -1.626773024405992 2216 46-47 -1.5576094155201403 2216 48-49 -1.7066137979630511 2216 50-51 -1.6046754477929142 2216 52-53 -1.7351118007071022 2216 54-55 -1.5994752962815753 2216 56-57 -1.748718988030312 2216 58-59 -1.8171553843669273 2216 60-61 -1.9023025801712663 2216 62-63 -2.021900219831913 2216 64-65 -1.8846048065500156 2216 66-67 -1.9717009390311553 2216 68-69 -1.8031794419525227 2216 70-71 -1.8559264872558572 2216 72-73 -1.9194972300812552 2216 74-75 -1.8468239357804563 2216 76-77 -1.8916161611361773 2216 78-79 -2.0133944167344993 2216 80-81 -1.7803650399428612 2216 82-83 -1.8830221303831536 2216 84-85 -1.9381374102469167 2216 86-87 -1.9327032119185183 2216 88-89 -1.9234284719655008 2216 90-91 -1.9565903259877118 2216 92-93 -2.0374697895954306 2216 94-95 -1.9661707665532546 2216 96-97 -2.054040736806055 2216 98-99 -2.253155491120978 2216 100-101 -2.1582996651004365 2216 102-103 -2.116657393853526 2216 104-105 -2.125748512087103 2216 106-107 -1.9971887055424453 2216 108-109 -1.9552346052911211 2216 110-111 -2.0098821214466227 2216 112-113 -1.988745016730082 2216 114-115 -2.0308245338893194 2216 116-117 -2.1276275816814483 2216 118-119 -2.084524574159129 2216 120-121 -2.054621170543374 2216 122-123 -2.1525083152512217 2216 124-125 -2.1597996091281786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43707.0 3 8425.0 4 5347.0 5 4520.0 6 4772.0 7 5598.0 8 6148.0 9 6931.0 10 7659.0 11 8499.0 12 9056.0 13 9236.0 14 9175.0 15 8603.0 16 7997.0 17 7308.0 18 7074.0 19 7180.0 20 7482.0 21 8013.0 22 9465.0 23 11959.0 24 16360.0 25 21008.0 26 24304.0 27 27073.0 28 31515.0 29 38499.0 30 45731.0 31 56425.0 32 71217.0 33 92920.0 34 128558.0 35 197442.0 36 379092.0 37 605457.0 38 218318.0 39 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11615559970372 18.15923188382957 12.577188462683011 25.147424053783705 2 15.60321479789356 20.08159785371233 38.66074800349374 25.654439344900364 3 18.212254834050956 24.45939665928889 29.93272893313805 27.3956195735221 4 12.485161369240735 16.25507710527852 37.31708288968028 33.94267863580046 5 13.205196827404992 38.491881984656054 34.891686420079196 13.411234767859751 6 32.92147865407868 36.5449347204154 17.14764526798902 13.385941357516895 7 28.702784324972747 32.610745410309505 21.40269411345523 17.28377615126252 8 27.156100853947663 34.090863811772905 20.584628200797933 18.168407133481505 9 27.09025901007861 15.4351066309045 19.400296432478214 38.074337926538675 10-11 24.81829135144439 26.138060812252427 28.273413977417277 20.770233858885902 12-13 25.719149298943528 23.669401208535923 27.683112837149736 22.928336655370813 14-15 22.394817643142385 25.412461434178297 24.63373183717173 27.55898908550759 16-17 22.89237317034297 28.464950646982295 26.075323120489564 22.567353062185173 18-19 23.20520420760398 27.203062737611315 26.988915738344986 22.602817316439722 20-21 23.268235253781505 26.755092935149488 26.956289877419287 23.02038193364972 22-23 23.439826013872743 26.630876699749294 26.891077512135524 23.038219774242435 24-25 23.074034451588332 26.989116137315232 26.81314699816942 23.123702412927013 26-27 22.55615565916442 27.216122086927353 26.55623720365412 23.67148505025411 28-29 23.20081544716389 27.46087822732906 26.309823590052474 23.028482735454578 30-31 22.80080497440266 26.967198474561 26.25822424240599 23.973772308630355 32-33 22.51790099704424 27.22095954301695 26.1625483968041 24.098591063134705 34-35 23.72817511430208 27.066588025108462 25.944857008890267 23.260379851699195 36-37 23.913860332886692 26.35071898113914 25.999892509586108 23.735528176388062 38-39 23.11931344988191 26.837609213615636 26.070580113536984 23.972497222965465 40-41 23.43289608749025 26.589262345420895 26.51865188696489 23.459189680123966 42-43 23.17784938713765 26.489784855315374 26.132515411327816 24.199850346219158 44-45 23.020935241348685 26.722415828950446 26.716716895276534 23.53993203442434 46-47 23.85615373353126 27.11765381785564 25.743954127539233 23.282238321073866 48-49 23.186482345402677 27.15236712777871 25.738915172033234 23.922235354785382 50-51 23.391084693603815 27.053332363449012 26.182034931588404 23.373548011358768 52-53 23.874985231466358 26.536749903280583 26.24999710420909 23.33826776104397 54-55 22.756822951006527 26.98053577245077 26.472767271148566 23.789874005394143 56-57 23.07026728998809 27.209231211167896 26.24468918098715 23.47581231785686 58-59 22.831019868813843 26.45330344570999 26.492037509029064 24.223639176447094 60-61 22.21637657751325 26.95324843325911 26.856366717910653 23.974008271316986 62-63 22.74939333391249 26.81083022036892 27.080740161584572 23.359036284134017 64-65 22.503896026874997 26.96666826515547 26.4428041014906 24.08663160647893 66-67 22.38968206825677 27.11206980431477 26.57260795955264 23.92564016787582 68-69 23.184337481142112 26.27941737928171 27.000349357441777 23.535895782134396 70-71 22.943284485237037 26.341855436909643 26.462228537923206 24.252631539930118 72-73 22.657699910831298 26.65015960711098 26.51442580012635 24.177714681931374 74-75 22.709915661910944 27.048305882816276 26.48178929725209 23.759989158020687 76-77 23.02259605605716 26.533095306160366 26.78994132874206 23.654367309040413 78-79 23.026498074846984 26.59885000486501 26.732846214792406 23.641805705495607 80-81 23.394896574634938 26.651123537068948 26.473135861145984 23.480844027150127 82-83 23.00425076280447 26.72409099954432 26.696152583596906 23.5755056540543 84-85 22.71944782992146 26.996384457266657 26.811935484095155 23.472232228716727 86-87 23.292935442604552 27.01895628975096 26.25255932846557 23.43554893917891 88-89 22.85182892473756 27.004116378032144 26.200579369551036 23.94347532767926 90-91 22.54691148671189 27.06151080469995 26.574720152711368 23.816857555876794 92-93 23.02138457037073 26.409130112771184 26.96041830906008 23.609067007798004 94-95 22.456920936348485 26.10224533301456 27.45108532228389 23.98974840835306 96-97 22.97879674719904 25.54930919794498 27.34127792183242 24.130616133023555 98-99 22.59344639867452 26.215355119365412 26.800998651954227 24.390199830005844 100-101 22.497248985889325 25.8631697705828 27.41439423248444 24.225187011043438 102-103 22.162820717161008 25.588058683549182 27.57896536514126 24.670155234148545 104-105 21.907235183123813 26.243202138712856 27.053049669706468 24.79651300845686 106-107 22.386278889435392 26.04478298330965 26.563997584057002 25.004940543197957 108-109 22.352140430277615 25.775350846344846 26.876968290968602 24.99554043240894 110-111 22.31701700607148 26.057365838108726 27.240270992564476 24.38534616325532 112-113 22.387830800477325 26.25155303892912 27.38621260515084 23.97440355544271 114-115 22.142667340357978 26.702454780092378 26.984279223271912 24.170598656277736 116-117 22.156848890613507 26.464685543515404 27.214006475040975 24.164459090830114 118-119 21.885347631756126 26.751299918070686 26.84013586128336 24.52321658888983 120-121 21.7575992356048 27.02463246844599 27.091568020329504 24.126200275619706 122-123 21.827691761658162 26.964750341658945 27.091915349666706 24.115642547016186 124-125 21.847257014274696 26.938535426347016 27.151507962782045 24.06269959659624 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 425.0 1 408.0 2 338.0 3 251.5 4 221.5 5 209.0 6 183.5 7 171.0 8 173.0 9 163.5 10 143.0 11 132.0 12 140.5 13 144.5 14 149.0 15 189.0 16 208.5 17 222.5 18 266.5 19 353.5 20 501.5 21 677.5 22 1046.5 23 1606.5 24 2407.5 25 3788.0 26 5802.0 27 8731.0 28 11737.5 29 15198.5 30 19701.0 31 26480.5 32 38431.0 33 42784.5 34 39382.0 35 40958.5 36 46607.0 37 52675.0 38 56438.5 39 60060.0 40 63182.0 41 65832.5 42 68387.0 43 70820.0 44 72008.0 45 76677.5 46 80302.5 47 80636.5 48 80373.0 49 76633.5 50 74856.5 51 74842.0 52 75962.5 53 80611.5 54 85816.0 55 83759.0 56 77163.5 57 70948.0 58 67168.0 59 58646.0 60 43748.0 61 35115.0 62 31649.0 63 24153.0 64 15476.0 65 10954.0 66 8184.0 67 6626.0 68 5333.0 69 4024.0 70 2911.5 71 2004.0 72 1343.5 73 834.0 74 724.5 75 559.0 76 303.5 77 160.0 78 85.5 79 49.0 80 31.5 81 22.5 82 21.5 83 19.0 84 13.5 85 13.0 86 12.5 87 6.0 88 4.5 89 5.0 90 4.5 91 3.5 92 3.0 93 3.0 94 2.5 95 1.0 96 1.5 97 1.5 98 1.0 99 1.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044061782866684614 2 0.008710426055664256 3 0.0023166026743787916 4 0.00532818615107122 5 0.022146721567061246 6 0.028447880841371558 7 0.0033359078511054595 8 1.8532821395030331E-4 9 0.0 10-11 6.949808023136374E-5 12-13 8.803090162639407E-4 14-15 4.633205348757583E-4 16-17 2.3166026743787915E-4 18-19 1.1583013371893957E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 9.266410697515166E-5 28-29 3.7065642790060663E-4 30-31 1.621621872065154E-4 32-33 1.621621872065154E-4 34-35 7.876449092887891E-4 36-37 4.633205348757583E-5 38-39 2.548262941816671E-4 40-41 5.791506685946979E-4 42-43 8.339769627763649E-4 44-45 0.001598455845321366 46-47 4.633205348757583E-5 48-49 0.0 50-51 0.0 52-53 0.0012972974976521233 54-55 0.0030579155301800045 56-57 1.3899616046272748E-4 58-59 0.0012046333906769716 60-61 0.0014362936581148508 62-63 9.498070964953045E-4 64-65 0.0020617763801971246 66-67 0.003289575797617884 68-69 0.003938224546443945 70-71 0.0020154443267095482 72-73 0.002988417449948641 74-75 0.002571428968560458 76-77 0.0016679539255527297 78-79 0.002918919369717277 80-81 0.0017374520057840935 82-83 6.718147755698495E-4 84-85 5.791506685946979E-4 86-87 0.002409266781353943 88-89 0.005791506685946979 90-91 6.023166953384858E-4 92-93 0.0011351353104456078 94-95 0.0015752898185775782 96-97 3.9382245464439456E-4 98-99 0.001088803256958032 100-101 0.0017606180325278815 102-103 0.002084942406940912 104-105 0.0011814673639331836 106-107 0.007853283066144103 108-109 0.002409266781353943 110-111 0.002084942406940912 112-113 0.002988417449948641 114-115 0.0034517379848243994 116-117 0.0021081084336847 118-119 0.045660238712005974 120-121 0.013297299350934265 122-123 0.005258688070839857 124-125 0.003405405931336823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2158333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.38166948800639 #Duplication Level Percentage of deduplicated Percentage of total 1 80.9365982511531 50.48960121586988 2 10.524439300017155 13.130641879205108 3 3.6156688641134194 6.766543801775963 4 1.6699473535927531 4.166964154967761 5 0.9050640734574942 2.8229703947947065 6 0.543330338629132 2.0336312164301464 7 0.36382229156827545 1.5887089361486875 8 0.24596315926290307 1.2274874005891436 9 0.19624403269819535 1.1017827348095104 >10 0.9077125918036855 9.989897780171912 >50 0.061169211205655466 2.6299371527043114 >100 0.02896123197768723 3.113048661014003 >500 8.994171005945829E-4 0.34090698446980117 >1k 0.0 0.0 >5k 1.7988342011891658E-4 0.5978776870490785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 5328 0.246857180981804 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.633205348757583E-5 0.0 0.0 0.0 0.0 8 4.633205348757583E-5 0.0 0.0 0.0 0.0 9 4.633205348757583E-5 0.0 0.0 0.0 0.0 10-11 4.633205348757583E-5 0.0 0.0 0.0 0.0 12-13 4.633205348757583E-5 0.0 0.0 0.0 4.633205348757583E-5 14-15 4.633205348757583E-5 0.0 0.0 2.3166026743787914E-5 6.949808023136374E-5 16-17 4.633205348757583E-5 0.0 0.0 1.1583013371893957E-4 9.266410697515166E-5 18-19 4.633205348757583E-5 0.0 0.0 1.3899616046272748E-4 9.266410697515166E-5 20-21 4.633205348757583E-5 0.0 0.0 1.3899616046272748E-4 2.3166026743787915E-4 22-23 4.633205348757583E-5 0.0 0.0 2.7799232092545496E-4 2.3166026743787915E-4 24-25 4.633205348757583E-5 0.0 0.0 6.486487488260616E-4 2.3166026743787915E-4 26-27 4.633205348757583E-5 0.0 0.0 0.001343629551139699 2.3166026743787915E-4 28-29 4.633205348757583E-5 0.0 0.0 0.004517375215038643 2.3166026743787915E-4 30-31 4.633205348757583E-5 0.0 0.0 0.01700386362994033 2.3166026743787915E-4 32-33 4.633205348757583E-5 0.0 0.0 0.04457143545504795 2.3166026743787915E-4 34-35 4.633205348757583E-5 0.0 0.0 0.08464866172180105 2.3166026743787915E-4 36-37 4.633205348757583E-5 0.0 0.0 0.15115832450321615 2.3166026743787915E-4 38-39 4.633205348757583E-5 0.0 0.0 0.25936683542344946 2.3166026743787915E-4 40-41 4.633205348757583E-5 0.0 0.0 0.3736680113772991 2.3166026743787915E-4 42-43 4.633205348757583E-5 0.0 0.0 0.48803868541137996 2.3166026743787915E-4 44-45 4.633205348757583E-5 0.0 0.0 0.6317375493030964 2.548262941816671E-4 46-47 4.633205348757583E-5 0.0 0.0 0.8049267652396548 2.7799232092545496E-4 48-49 4.633205348757583E-5 0.0 0.0 0.9835368314342597 2.7799232092545496E-4 50-51 4.633205348757583E-5 0.0 0.0 1.1881623456621384 3.243243744130308E-4 52-53 4.633205348757583E-5 0.0 0.0 1.378911409870488 3.243243744130308E-4 54-55 4.633205348757583E-5 0.0 0.0 1.5770967686635937 3.243243744130308E-4 56-57 4.633205348757583E-5 0.0 0.0 1.8081547194061343 3.243243744130308E-4 58-59 4.633205348757583E-5 0.0 0.0 2.052440471419378 3.243243744130308E-4 60-61 4.633205348757583E-5 0.0 0.0 2.3277455332425534 3.243243744130308E-4 62-63 4.633205348757583E-5 0.0 0.0 2.5935061920472884 3.243243744130308E-4 64-65 4.633205348757583E-5 0.0 0.0 2.8683479333355884 3.243243744130308E-4 66-67 4.633205348757583E-5 0.0 0.0 3.197768833632252 3.243243744130308E-4 68-69 4.633205348757583E-5 0.0 0.0 3.553228347988934 3.243243744130308E-4 70-71 4.633205348757583E-5 0.0 0.0 3.9350276347533026 3.243243744130308E-4 72-73 4.633205348757583E-5 0.0 0.0 4.33566090126037 3.243243744130308E-4 74-75 4.633205348757583E-5 0.0 0.0 4.725174474930421 3.243243744130308E-4 76-77 4.633205348757583E-5 0.0 0.0 5.1552285954021 3.243243744130308E-4 78-79 4.633205348757583E-5 0.0 0.0 5.64479623857857 3.474904011568187E-4 80-81 4.633205348757583E-5 0.0 0.0 6.150950756903592 3.7065642790060663E-4 82-83 4.633205348757583E-5 0.0 0.0 6.690788678114082 3.7065642790060663E-4 84-85 4.633205348757583E-5 0.0 0.0 7.236024283555874 3.7065642790060663E-4 86-87 4.633205348757583E-5 0.0 0.0 7.7873525540312825 3.9382245464439456E-4 88-89 4.633205348757583E-5 0.0 0.0 8.367777354096887 4.1698848138818244E-4 90-91 4.633205348757583E-5 0.0 0.0 9.001854672101107 4.1698848138818244E-4 92-93 4.633205348757583E-5 0.0 0.0 9.639267897956433 4.1698848138818244E-4 94-95 4.633205348757583E-5 0.0 0.0 10.284603904958132 4.1698848138818244E-4 96-97 4.633205348757583E-5 0.0 0.0 10.967237215017331 4.8648656161954623E-4 98-99 4.633205348757583E-5 0.0 0.0 11.649453536595141 5.096525883633342E-4 100-101 4.633205348757583E-5 0.0 0.0 12.390164075701016 5.096525883633342E-4 102-103 4.633205348757583E-5 0.0 0.0 13.16682828831325 5.096525883633342E-4 104-105 4.633205348757583E-5 0.0 0.0 13.948450030648655 5.096525883633342E-4 106-107 4.633205348757583E-5 0.0 0.0 14.725762892009714 5.096525883633342E-4 108-109 4.633205348757583E-5 0.0 0.0 15.548064177307209 5.096525883633342E-4 110-111 4.633205348757583E-5 0.0 0.0 16.40196392308323 5.096525883633342E-4 112-113 4.633205348757583E-5 0.0 0.0 17.283732399032033 5.096525883633342E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2585 0.0 54.582115 1 GGTATCA 1555 0.0 50.53661 1 ATCAACG 2960 0.0 46.64962 3 TCAACGC 2995 0.0 46.10447 4 CAACGCA 3115 0.0 44.519444 5 AACGCAG 3325 0.0 41.88669 6 TATCAAC 3395 0.0 40.3218 2 GTACATG 7745 0.0 35.973854 1 ACGCAGA 3925 0.0 35.622887 7 TACATGG 7925 0.0 34.4719 2 ACATGGG 7765 0.0 34.18576 3 CGCAGAG 4040 0.0 34.01978 8 TAAGGTA 895 0.0 33.25055 4 GTGTAAG 960 0.0 31.627258 1 TAAGGTG 1770 0.0 30.93616 5 AGGTAAG 1540 0.0 30.145723 2 CATGGGG 4320 0.0 28.243723 4 CATGGGA 3940 0.0 27.342247 4 GGTAAGG 1825 0.0 26.416422 3 TGTAAGG 1180 0.0 25.72409 2 >>END_MODULE