FastQCFastQC Report
Thu 26 May 2016
SRR1364356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158333
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23030.10670271918188712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22550.073.9369661
GTATCAA43100.054.710171
ATCAACG55100.042.245913
TCAACGC55100.042.1378634
TATCAAC56750.041.4372332
CAACGCA56350.041.2031335
AACGCAG59550.039.0699776
TAAGGTA10050.037.3193444
GTACATG76400.034.7609521
AGGTAAG16100.034.7585372
TACATGG78000.034.193452
ACATGGG75000.033.9736373
GTGTAAG9650.033.9379431
ACGCAGA69600.033.339057
CGCAGAG71250.032.399988
CATGGGA39800.029.6170394
TAAGGTG20250.029.1051395
GGTAAGG20600.028.0326423
TGTAAGG12800.027.9062082
GTGGTAT12550.027.5191121
Produced by FastQC (version 0.11.5)