FastQCFastQC Report
Thu 26 May 2016
SRR1364355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380813
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26150.045.993251
ATCAACG28300.041.859093
GGTATCA18650.040.864441
CAACGCA29200.040.5689135
GTACATG72450.038.707661
TCAACGC30600.038.5182844
AACGCAG31250.037.7068026
TACATGG77100.036.28832
TATCAAC34150.034.1655542
ACATGGG79750.033.813543
ACGCAGA37750.031.5209267
CATGGGG50550.030.7356574
CGCAGAG38550.030.7105278
CGTATAG2205.124093E-927.0639861
ATGGGGA32000.023.06725
CATGGGA36700.021.8973144
GCAGAGT55500.020.79549
GAGTACA58450.020.7806951
AGTACAT57350.019.825452
GGACCGA7050.019.415266
Produced by FastQC (version 0.11.5)