##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364355_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2380813 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.902154852145046 31.0 31.0 34.0 27.0 34.0 2 30.98043945492569 33.0 31.0 34.0 27.0 34.0 3 31.052634541226045 33.0 31.0 34.0 27.0 34.0 4 34.52524746798677 37.0 35.0 37.0 32.0 37.0 5 34.58511021235183 37.0 35.0 37.0 32.0 37.0 6 34.595386533927694 37.0 35.0 37.0 31.0 37.0 7 34.56496289292775 37.0 35.0 37.0 31.0 37.0 8 34.50499430236646 37.0 35.0 37.0 31.0 37.0 9 36.03971164471968 39.0 37.0 39.0 31.0 39.0 10-11 35.95310593482142 39.0 37.0 39.0 30.5 39.0 12-13 35.96870039772128 39.0 37.0 39.0 31.0 39.0 14-15 37.23737626600661 40.0 38.0 41.0 31.0 41.0 16-17 37.23123676660032 40.0 38.0 41.0 31.0 41.0 18-19 37.21495451343722 40.0 37.5 41.0 31.0 41.0 20-21 37.228580321092 40.0 38.0 41.0 31.0 41.0 22-23 37.162879654975 40.0 37.5 41.0 30.5 41.0 24-25 37.145419862878775 40.0 37.0 41.0 30.5 41.0 26-27 37.07889468849507 40.0 37.0 41.0 30.0 41.0 28-29 37.070105253961565 40.0 37.0 41.0 30.0 41.0 30-31 36.965622457538664 40.0 37.0 41.0 30.0 41.0 32-33 36.919482756520566 40.0 37.0 41.0 30.0 41.0 34-35 36.9052600939259 40.0 37.0 41.0 30.0 41.0 36-37 36.84676536964474 40.0 37.0 41.0 30.0 41.0 38-39 36.752537683556 40.0 37.0 41.0 30.0 41.0 40-41 36.69660027898033 40.0 37.0 41.0 30.0 41.0 42-43 36.56494168168605 40.0 36.5 41.0 30.0 41.0 44-45 36.42595050514257 40.0 36.0 41.0 29.5 41.0 46-47 36.315683130090434 40.0 36.0 41.0 29.0 41.0 48-49 36.17276745380674 40.0 36.0 41.0 29.0 41.0 50-51 36.04553276548809 40.0 35.0 41.0 28.5 41.0 52-53 35.889324571060385 39.0 35.0 41.0 28.0 41.0 54-55 35.713418693530315 39.0 35.0 41.0 28.0 41.0 56-57 35.518898586323246 39.0 35.0 41.0 28.0 41.0 58-59 35.29808683000303 39.0 35.0 40.5 27.5 41.0 60-61 35.051686965754975 38.0 34.0 40.0 27.0 41.0 62-63 34.788427734559576 38.0 34.0 40.0 26.0 41.0 64-65 34.453215561238956 37.0 34.0 40.0 26.0 41.0 66-67 34.141251538865085 37.0 34.0 40.0 26.0 41.0 68-69 33.82015198169701 36.0 33.0 39.0 26.0 41.0 70-71 33.46837088843181 36.0 33.0 39.0 26.0 41.0 72-73 33.10581217424468 35.0 33.0 38.5 25.0 40.0 74-75 32.7415838623193 35.0 32.5 37.5 24.5 39.5 76-77 32.513260806287605 35.0 32.5 37.0 24.5 39.0 78-79 32.35766647779561 35.0 33.0 37.0 25.0 39.0 80-81 32.090301086225594 35.0 33.0 36.0 24.5 38.5 82-83 31.793327951418277 35.0 32.0 36.0 24.0 37.0 84-85 31.546642260437928 35.0 32.0 35.5 23.5 37.0 86-87 31.339588619517787 35.0 32.0 35.0 23.0 36.5 88-89 31.08094734865779 35.0 32.0 35.0 21.0 36.0 90-91 30.954308255205262 35.0 32.0 35.0 20.5 36.0 92-93 30.81440541529301 35.0 32.0 35.0 20.0 36.0 94-95 30.717554885663006 35.0 32.0 35.0 20.0 35.0 96-97 30.616264906147606 35.0 32.0 35.0 19.5 35.0 98-99 30.48895167323095 35.0 31.5 35.0 19.0 35.0 100-101 30.40511539545525 35.0 31.0 35.0 18.0 35.0 102-103 29.372934581590407 33.5 29.5 34.5 16.5 35.0 104-105 30.10102032372975 34.0 31.0 35.0 17.0 35.0 106-107 30.469187206219054 35.0 32.0 35.0 18.0 35.0 108-109 30.52594260868031 35.0 32.0 35.0 18.0 35.0 110-111 30.466721031849204 35.0 32.0 35.0 17.0 35.0 112-113 30.37358918991118 35.0 32.0 35.0 12.5 35.0 114-115 30.283661505544536 35.0 32.0 35.0 8.5 35.0 116-117 30.16060081157151 35.0 32.0 35.0 2.0 35.0 118-119 29.991986140868686 35.0 32.0 35.0 2.0 35.0 120-121 29.816211100997855 35.0 32.0 35.0 2.0 35.0 122-123 29.63527626907279 35.0 31.0 35.0 2.0 35.0 124-125 28.786323621384796 34.5 30.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2901392743535851 1101 2 0.24963220118323548 1101 3 0.30972819420257736 1101 4 0.34795195765903486 1101 5 0.3158523258890398 1101 6 0.27950262143536975 1101 7 0.34372119609370344 1101 8 0.1515677652619587 1101 9 0.36733261371844605 1101 10-11 0.5022549478210436 1101 12-13 0.5110980771984757 1101 14-15 0.6335849460945724 1101 16-17 0.5578356732030727 1101 18-19 0.3708491338323441 1101 20-21 0.49283368491076374 1101 22-23 0.6984828640748191 1101 24-25 0.6358476604617778 1101 26-27 0.7045054179148593 1101 28-29 0.6719930042306572 1101 30-31 0.7232839226858871 1101 32-33 0.5279699506402267 1101 34-35 0.5671606357532397 1101 36-37 0.5411013691828401 1101 38-39 0.653892160028029 1101 40-41 0.5333849603373366 1101 42-43 0.619900221272701 1101 44-45 0.5838674652752331 1101 46-47 0.5912520714475917 1101 48-49 0.463664957978942 1101 50-51 0.4278569045986984 1101 52-53 0.44935558908002804 1101 54-55 0.49871620529329164 1101 56-57 0.4063182262469809 1101 58-59 0.4697254819489274 1101 60-61 0.3891279151658438 1101 62-63 0.47179408814213275 1101 64-65 0.5332785749677811 1101 66-67 0.5576271420225964 1101 68-69 0.489524223333035 1101 70-71 0.4851300375665062 1101 72-73 0.46038811585518147 1101 74-75 0.46522214538551765 1101 76-77 0.5071312464505979 1101 78-79 0.48279186873854485 1101 80-81 0.43094805621819887 1101 82-83 0.4008873338299246 1101 84-85 0.4324549641626909 1101 86-87 0.3846952472897698 1101 88-89 0.5072860026682307 1101 90-91 0.35276643338805513 1101 92-93 0.37531839604393724 1101 94-95 0.3293791907619301 1101 96-97 0.38410620577640486 1101 98-99 0.40669900385222135 1101 100-101 0.3510930217897865 1101 102-103 0.16013280974538802 1101 104-105 0.335427366034029 1101 106-107 0.37507441384531504 1101 108-109 0.3179779373639384 1101 110-111 0.44246561822517094 1101 112-113 0.43683821496569664 1101 114-115 0.37970043170142276 1101 116-117 0.2621899655032216 1101 118-119 0.2690406390945057 1101 120-121 0.08396166530544491 1101 122-123 -0.028639016685694685 1101 124-125 -0.034304009776388256 1102 1 0.31367963345591576 1102 2 0.3479652404458733 1102 3 0.40167989939250504 1102 4 0.44633820971402827 1102 5 0.380652903948004 1102 6 0.40225893205244745 1102 7 0.3843295583439854 1102 8 0.4230419276505941 1102 9 0.44144683077622204 1102 10-11 0.492105307847595 1102 12-13 0.4183940411482112 1102 14-15 0.4834968649198572 1102 16-17 0.6058080557638448 1102 18-19 0.5717491301914635 1102 20-21 0.5757931283609707 1102 22-23 0.6072918917650796 1102 24-25 0.6225668677592111 1102 26-27 0.6162971626502909 1102 28-29 0.6533482220250164 1102 30-31 0.7496946218179588 1102 32-33 0.7455350032788743 1102 34-35 0.6384654274251602 1102 36-37 0.5784199905794196 1102 38-39 0.6413045339627246 1102 40-41 0.5981599822526036 1102 42-43 0.5239256989975445 1102 44-45 0.5596202862533914 1102 46-47 0.5082408155416687 1102 48-49 0.5279148536538685 1102 50-51 0.5252968791685859 1102 52-53 0.5426085570935371 1102 54-55 0.5504124330664482 1102 56-57 0.5537659726852269 1102 58-59 0.5172956178095802 1102 60-61 0.46449158259149215 1102 62-63 0.5822620946508934 1102 64-65 0.5707162249788382 1102 66-67 0.5153572803478568 1102 68-69 0.4624896742274345 1102 70-71 0.3971671912982657 1102 72-73 0.5381880189518853 1102 74-75 0.4422468221178093 1102 76-77 0.49386025610451867 1102 78-79 0.5866649898368621 1102 80-81 0.541175204824718 1102 82-83 0.4834077717431988 1102 84-85 0.4347931762688866 1102 86-87 0.46415875031589593 1102 88-89 0.5566552210283255 1102 90-91 0.47557385629252025 1102 92-93 0.42635506525921585 1102 94-95 0.3580017216183471 1102 96-97 0.2649820183217422 1102 98-99 0.2483485864118684 1102 100-101 0.22934880447994743 1102 102-103 0.03137710687441597 1102 104-105 0.16581859208477212 1102 106-107 0.17526527972947648 1102 108-109 0.27462911630735576 1102 110-111 0.3063091362068917 1102 112-113 0.308302014863191 1102 114-115 0.30206331773922557 1102 116-117 0.23269082390958573 1102 118-119 0.20666159317186583 1102 120-121 0.048111997045197086 1102 122-123 0.022629408243663107 1102 124-125 0.09216249277082511 1103 1 0.3987175067262996 1103 2 0.44441045133228485 1103 3 0.4476446274299519 1103 4 0.4350864816076623 1103 5 0.38395717833044074 1103 6 0.3350387029109285 1103 7 0.37156863115762917 1103 8 0.38149797814860165 1103 9 0.4812260215925619 1103 10-11 0.6702738147092191 1103 12-13 0.5534013964805098 1103 14-15 0.6767689681611984 1103 16-17 0.5934059541591239 1103 18-19 0.5913658760484495 1103 20-21 0.6271876098366818 1103 22-23 0.6781328249768634 1103 24-25 0.7520609805327609 1103 26-27 0.7596633776704209 1103 28-29 0.7595816737115797 1103 30-31 0.8828319392758033 1103 32-33 0.8271051766543991 1103 34-35 0.8601008573183435 1103 36-37 0.7604046015805181 1103 38-39 0.6382230156591859 1103 40-41 0.7740822172883313 1103 42-43 0.7287362355377098 1103 44-45 0.6596382393748996 1103 46-47 0.5783225306663695 1103 48-49 0.6356177138608672 1103 50-51 0.6496870237101646 1103 52-53 0.6115985187406565 1103 54-55 0.631690216883861 1103 56-57 0.5636860197138773 1103 58-59 0.5787571108508587 1103 60-61 0.5138792461443131 1103 62-63 0.49233187734470363 1103 64-65 0.552340328773461 1103 66-67 0.5423682016722111 1103 68-69 0.45251136245087054 1103 70-71 0.6291955363875701 1103 72-73 0.6163090850451951 1103 74-75 0.5914346565331883 1103 76-77 0.5525128435448678 1103 78-79 0.6301785720364563 1103 80-81 0.641563352259162 1103 82-83 0.5699163562011513 1103 84-85 0.5346755335847924 1103 86-87 0.4306370986855619 1103 88-89 0.5439061362704081 1103 90-91 0.4897863099323736 1103 92-93 0.5190555818496314 1103 94-95 0.5006813126377132 1103 96-97 0.48800703876769447 1103 98-99 0.5872647957831596 1103 100-101 0.5960171527714486 1103 102-103 0.27772342164095676 1103 104-105 0.48838727639334323 1103 106-107 0.49300320063010616 1103 108-109 0.5438473052353032 1103 110-111 0.5404772896904504 1103 112-113 0.40745357592026465 1103 114-115 0.44523508008442647 1103 116-117 0.5116143566386206 1103 118-119 0.460144155314417 1103 120-121 0.18461007726912015 1103 122-123 0.13640056563024672 1103 124-125 0.10067708039259671 1104 1 0.35186555998217983 1104 2 0.39714581766276424 1104 3 0.42181567150844756 1104 4 0.48470257267401706 1104 5 0.372681305458741 1104 6 0.37319006300496227 1104 7 0.46434134816433215 1104 8 0.484242370487749 1104 9 0.6361080399214316 1104 10-11 0.5858559841731363 1104 12-13 0.511480726596865 1104 14-15 0.5785386325151052 1104 16-17 0.6461231875215177 1104 18-19 0.6485256056405646 1104 20-21 0.6876364493672042 1104 22-23 0.702125332175406 1104 24-25 0.6660610890067744 1104 26-27 0.6872638598130933 1104 28-29 0.6673314937985424 1104 30-31 0.7155829938617018 1104 32-33 0.6508833667413327 1104 34-35 0.6500258078776682 1104 36-37 0.6422019349008963 1104 38-39 0.44903617404175833 1104 40-41 0.5272734982449094 1104 42-43 0.4431185125042276 1104 44-45 0.4215332640608338 1104 46-47 0.5085800188131628 1104 48-49 0.37514802951212545 1104 50-51 0.38380420315935737 1104 52-53 0.23595105440148245 1104 54-55 0.37930646013762725 1104 56-57 0.2732538722280822 1104 58-59 0.31242618742136585 1104 60-61 0.18086206596656496 1104 62-63 0.3076741897352733 1104 64-65 0.41290626356439475 1104 66-67 0.37612672882187326 1104 68-69 0.25333111022451504 1104 70-71 0.20934774808350198 1104 72-73 0.24391066002299056 1104 74-75 0.20738648759868994 1104 76-77 0.2487447075411282 1104 78-79 0.3824152817446347 1104 80-81 0.3385302895441953 1104 82-83 0.2245962809817108 1104 84-85 0.21756274733282055 1104 86-87 0.27852659119721324 1104 88-89 0.2661861708201876 1104 90-91 0.19395345218469728 1104 92-93 0.15990648756709902 1104 94-95 0.24759886996500668 1104 96-97 0.19653325750147843 1104 98-99 0.196826617466467 1104 100-101 0.13566986595727215 1104 102-103 -0.029135697339647493 1104 104-105 0.053031631414189206 1104 106-107 0.09204579610544528 1104 108-109 0.12748027777114146 1104 110-111 0.19157643897901266 1104 112-113 0.23326362984155935 1104 114-115 0.09108028282153313 1104 116-117 0.11771241713959668 1104 118-119 0.14552736402406907 1104 120-121 -0.00819222723684021 1104 122-123 0.011978614700971946 1104 124-125 -0.06418304885336923 1105 1 -0.05010039312767489 1105 2 0.0198931710400565 1105 3 -0.12049849017405023 1105 4 -0.0730943797346697 1105 5 0.04741702634014189 1105 6 0.13192190638773127 1105 7 0.029749232666979708 1105 8 0.12739563513469676 1105 9 0.14438182504210317 1105 10-11 0.06219308899969889 1105 12-13 0.15630185206765645 1105 14-15 0.18988529698503953 1105 16-17 0.1322304777553569 1105 18-19 0.05908642527940344 1105 20-21 0.12927894552611008 1105 22-23 0.22885210262518996 1105 24-25 0.2381710689679153 1105 26-27 0.1665698182104407 1105 28-29 0.17720570498428145 1105 30-31 0.10612948361430341 1105 32-33 0.192700771805832 1105 34-35 0.10002983097407281 1105 36-37 0.027173562492443182 1105 38-39 0.03672090121834515 1105 40-41 0.04614373614401046 1105 42-43 0.034795300770575466 1105 44-45 0.04844931161765942 1105 46-47 0.04566396359328451 1105 48-49 -0.0463929696717571 1105 50-51 0.11809401169828249 1105 52-53 0.14653582104273255 1105 54-55 0.06038737035361663 1105 56-57 0.05074809621110887 1105 58-59 0.014524519560538351 1105 60-61 -0.02879432328525411 1105 62-63 0.02141306395795084 1105 64-65 0.008338133384654611 1105 66-67 -0.1145126753000767 1105 68-69 -0.14694226875499083 1105 70-71 -0.22819521283882693 1105 72-73 -0.22711863961340129 1105 74-75 -0.1833282058988388 1105 76-77 -0.04870177569788581 1105 78-79 -3.7582759546239686E-4 1105 80-81 -0.06265441655904169 1105 82-83 -0.09325419776676114 1105 84-85 -0.06016558254576054 1105 86-87 -0.14850746718749264 1105 88-89 -0.10271433608996006 1105 90-91 -0.11411822023563545 1105 92-93 -0.13530679401516466 1105 94-95 -0.24010558954156735 1105 96-97 -0.2270827015472321 1105 98-99 -0.26667125412395976 1105 100-101 -0.43016006051585265 1105 102-103 -0.5097918835208901 1105 104-105 -0.3760806436276667 1105 106-107 -0.35204963940432066 1105 108-109 -0.3339570021559055 1105 110-111 -0.33833604909538195 1105 112-113 -0.3264511900961402 1105 114-115 -0.4334650114967076 1105 116-117 -0.39603488642793394 1105 118-119 -0.4303011064713047 1105 120-121 -0.5440765229842341 1105 122-123 -0.5041972980629694 1105 124-125 -0.6037605471004532 1106 1 0.3920602666724733 1106 2 0.422277448174615 1106 3 0.3900763516453587 1106 4 0.4582720417951194 1106 5 0.3996429644147099 1106 6 0.36293756951947387 1106 7 0.391738163661131 1106 8 0.4890395126351095 1106 9 0.5748083542640785 1106 10-11 0.5284875448394573 1106 12-13 0.45220573076871773 1106 14-15 0.5844858474150811 1106 16-17 0.46515273482431496 1106 18-19 0.543146065020288 1106 20-21 0.4533764986441753 1106 22-23 0.48725246236973874 1106 24-25 0.5126762990228428 1106 26-27 0.6224792974123403 1106 28-29 0.6030767003185673 1106 30-31 0.7131162762015038 1106 32-33 0.5801633310408221 1106 34-35 0.5315064727467274 1106 36-37 0.5436706542962852 1106 38-39 0.458771810591287 1106 40-41 0.48577317091476147 1106 42-43 0.47487217901711176 1106 44-45 0.40831765883231697 1106 46-47 0.4187075076737443 1106 48-49 0.48502513157948357 1106 50-51 0.3566194915534453 1106 52-53 0.41883161250125767 1106 54-55 0.4429833479113441 1106 56-57 0.518448046582165 1106 58-59 0.5427271801340225 1106 60-61 0.36505232217507455 1106 62-63 0.4009975232020011 1106 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-1.7129593024219218 2216 90-91 -1.9081447460951395 2216 92-93 -1.7974845838122206 2216 94-95 -1.6588332276816828 2216 96-97 -1.6322178308982487 2216 98-99 -1.6369793735584217 2216 100-101 -1.488478818310277 2216 102-103 -1.5789223512935422 2216 104-105 -1.694019938048367 2216 106-107 -1.5003899735359845 2216 108-109 -1.506243763156629 2216 110-111 -1.5451184899647359 2216 112-113 -1.5791457030137295 2216 114-115 -1.6052439795290958 2216 116-117 -1.6296179008504232 2216 118-119 -1.7325332554083488 2216 120-121 -1.449325857113955 2216 122-123 -1.4647526795983765 2216 124-125 -1.3042111404010868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45419.0 3 8351.0 4 5218.0 5 4444.0 6 4704.0 7 5375.0 8 6023.0 9 6804.0 10 7530.0 11 8310.0 12 8651.0 13 8722.0 14 8625.0 15 8238.0 16 7780.0 17 7358.0 18 6705.0 19 6753.0 20 7006.0 21 7423.0 22 8720.0 23 11318.0 24 15197.0 25 20443.0 26 24621.0 27 27558.0 28 32571.0 29 39223.0 30 46693.0 31 58527.0 32 74117.0 33 97680.0 34 135472.0 35 203858.0 36 393052.0 37 719517.0 38 292380.0 39 427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.419041111663 18.737125553743013 12.831625985307479 25.01220734928651 2 15.495207734834015 20.19963815951142 39.481905245174865 24.8232488604797 3 18.066056439309932 24.87797973077414 29.802222155016565 27.253741674899363 4 12.208749914940483 16.414470450954052 38.2611986629064 33.11558097119906 5 12.965060442418167 38.413305650338906 35.65123796256074 12.970395944682183 6 32.20386835802121 37.3139376258044 17.582597640601584 12.899596375572802 7 28.278211677575065 32.24879385937278 22.163248813181145 17.309745649871005 8 25.972525340094048 35.443369741079934 20.605238884884653 17.97886603394136 9 26.582432135577218 15.69598284283562 19.76665113975772 37.95493388182945 10-11 24.502979550048565 26.417599033943244 28.861365605229306 20.218055810778885 12-13 25.2521001344086 23.82986811155914 28.360152049731184 22.557879704301076 14-15 22.299954952258844 25.491015128270554 25.456699040136595 26.752330879334007 16-17 22.642712054058958 28.459770800501342 26.384844976999407 22.512672168440297 18-19 22.737676938168747 27.746370406212506 27.236208005635053 22.279744649983694 20-21 23.2400444722034 27.167379378388812 27.147050188318023 22.445525961089764 22-23 23.253380252880003 26.936008833957143 26.92481517868056 22.885795734482297 24-25 22.91332834624139 27.122793768347197 26.996849395563615 22.967028489847795 26-27 22.739253931168456 27.449116635602344 26.77991231984879 23.031717113380413 28-29 22.898762624666315 27.474566906032432 26.820943584009697 22.805726885291552 30-31 23.008261894061267 27.17759922043338 26.767171676866276 23.046967208639078 32-33 22.720029200158436 27.294986704099323 26.804607173443983 23.180376922298258 34-35 23.38924602916253 27.178988531141908 26.55835550729903 22.873409932396534 36-37 23.282497736066517 26.97573348924653 26.63225823794571 23.109510536741247 38-39 22.842725197994547 27.21327463903574 26.7137178704479 23.23028229252181 40-41 23.22185143260368 26.848019892431342 26.750048828022454 23.180079846942526 42-43 22.80190389864915 26.869361924295166 26.782668339494432 23.546065837561255 44-45 22.880557698289074 27.069217281439258 26.787881562618725 23.26234345765295 46-47 23.23860026776573 27.297067702727535 26.47465938623894 22.98967264326779 48-49 22.997585278642212 27.071928790711407 26.701194087901907 23.229291842744473 50-51 22.91526045934729 27.21960943593638 26.772325251920247 23.092804852796085 52-53 23.117880656176187 26.986947839029295 26.579562654215916 23.3156088505786 54-55 22.890443810798715 27.014260809226315 26.6927452231025 23.402550156872472 56-57 22.855190341612513 27.13658767189255 26.826273310591787 23.18194867590315 58-59 22.836349095775297 26.83767806278277 26.786308699630563 23.539664141811365 60-61 22.69344516171306 27.020536451960712 26.899799268394414 23.386219117931812 62-63 22.83746696483025 27.006219768514395 27.027242149264364 23.12907111739099 64-65 22.82154399755206 26.789433438572196 26.86596315610739 23.523059407768354 66-67 22.627295575320115 27.014844281115792 26.78844489453632 23.569415249027774 68-69 22.72178455475704 26.737070561478905 27.18336046425911 23.35778441950495 70-71 22.720676318437885 26.573544211709475 26.953147970490242 23.75263149936239 72-73 22.755463763888198 26.868321310879097 26.611595866602737 23.764619058629968 74-75 22.683656149467797 26.973492156099272 26.69383438194498 23.64901731248795 76-77 22.786907349085553 26.70105586978418 26.950091272378113 23.561945508752153 78-79 22.748676315824294 26.637223693857486 27.05263717202969 23.561462818288526 80-81 22.91289996515856 26.75163302195851 26.87465964489451 23.460807367988423 82-83 22.667455196801413 26.801295026337772 26.983124587061248 23.548125189799567 84-85 22.75205046364609 26.674613565255083 27.007967479879685 23.56536849121914 86-87 22.823945244490744 26.56146238708986 27.091838127660644 23.522754240758747 88-89 22.738402021258498 26.62041966736604 27.060627253806135 23.580551057569323 90-91 22.703170861608097 26.720931248435402 27.023917130515574 23.55198075944093 92-93 22.75827945237471 26.27446747159607 27.153671987242884 23.813581088786343 94-95 22.483916073341952 26.33448183021371 27.236744227658598 23.94485786878574 96-97 22.63997515127752 26.069821297950924 27.376332347882954 23.9138712028886 98-99 22.58593413156927 26.145212632017547 27.11728431490116 24.151568921512027 100-101 22.480131419721946 26.03320607753209 27.387369375189273 24.099293127556685 102-103 22.264052592858768 25.86678018690937 27.521301321688966 24.347865898542892 104-105 22.164046043443474 26.16751967305039 27.30201466316923 24.366419620336906 106-107 22.2785233369941 25.990280391733105 27.227882787547863 24.503313483724938 108-109 22.262708571788597 26.01728428767943 27.13171131249278 24.58829582803919 110-111 22.28819270671337 26.166818962788852 27.258646698968427 24.28634163152935 112-113 22.298089829047964 26.24540823280677 27.498581857733818 23.957920080411448 114-115 22.137829747580742 26.38646404912083 27.258440280055606 24.217265923242824 116-117 22.09691686947288 26.217152323499786 27.42636601222634 24.25956479480099 118-119 21.90680115256821 26.286522541012026 27.282980541061608 24.523695765358156 120-121 21.88343698737458 26.398237356202337 27.46002440648347 24.258301249939613 122-123 21.953251095529776 26.50168700559395 27.405308738300434 24.13975316057584 124-125 21.825120051580438 26.469522702428545 27.441360329056312 24.2639969169347 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 230.0 1 219.0 2 179.0 3 125.5 4 113.0 5 109.0 6 90.5 7 93.0 8 96.5 9 88.5 10 79.0 11 89.5 12 107.0 13 116.5 14 130.0 15 147.5 16 173.5 17 204.0 18 255.0 19 343.0 20 500.5 21 732.0 22 1050.5 23 1623.5 24 2549.0 25 3858.5 26 5739.0 27 8855.5 28 12779.0 29 17400.0 30 22548.0 31 28395.5 32 35735.5 33 42031.0 34 46410.0 35 51484.5 36 58891.0 37 65458.0 38 69933.5 39 74631.0 40 77527.0 41 80757.0 42 83204.5 43 83839.0 44 86069.5 45 89977.5 46 91202.0 47 90587.5 48 89406.5 49 88148.0 50 87431.0 51 85441.5 52 85107.0 53 86350.5 54 88849.5 55 83674.0 56 73273.5 57 66752.5 58 63129.0 59 56135.5 60 44843.5 61 36507.5 62 29506.0 63 22066.5 64 16292.0 65 12068.0 66 8358.5 67 6210.5 68 4605.5 69 3036.5 70 2095.5 71 1369.5 72 1008.5 73 818.5 74 599.5 75 391.0 76 241.0 77 138.0 78 74.5 79 46.0 80 35.0 81 24.5 82 18.0 83 13.5 84 11.0 85 9.0 86 11.5 87 12.0 88 10.0 89 7.0 90 4.0 91 5.5 92 7.5 93 7.5 94 5.0 95 3.5 96 2.0 97 1.0 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046160702247509566 2 0.008652506517731548 3 0.0025621499882603127 4 0.005334312270640323 5 0.022303305635511902 6 0.026461549059081917 7 0.0033181942470912247 8 4.200245882393955E-5 9 0.0 10-11 2.1001229411969775E-5 12-13 5.460319647112142E-4 14-15 2.3101352353166753E-4 16-17 2.1001229411969776E-4 18-19 1.0500614705984888E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.1001229411969775E-5 28-29 3.1501844117954664E-4 30-31 1.2600737647181867E-4 32-33 1.680098352957582E-4 34-35 7.56044258830912E-4 36-37 4.200245882393955E-5 38-39 1.0500614705984888E-4 40-41 3.360196705915164E-4 42-43 5.880344235351538E-4 44-45 0.0011550676176583377 46-47 2.1001229411969775E-5 48-49 0.0 50-51 0.0 52-53 8.190479470668213E-4 54-55 0.002415141382376524 56-57 6.300368823590934E-5 58-59 6.930405705950027E-4 60-61 9.240540941266701E-4 62-63 7.140418000069723E-4 64-65 0.0016170946647216726 66-67 0.0027091585941441012 68-69 0.003087180723559557 70-71 0.0017221008117815216 72-73 0.0024991463000244036 74-75 0.0019321131059012195 76-77 0.001134066388246368 78-79 0.0022051290882568264 80-81 0.0011550676176583377 82-83 5.040295058872747E-4 84-85 4.200245882393955E-4 86-87 0.0020161180235490988 88-89 0.004956290141224867 90-91 3.78022129415456E-4 92-93 0.0010290602411865192 94-95 8.40049176478791E-4 96-97 2.730159823556071E-4 98-99 9.030528647147005E-4 100-101 0.0018481081882533402 102-103 0.001407082370601975 104-105 7.56044258830912E-4 106-107 0.0067833971000662375 108-109 0.0015960934353097028 110-111 0.0014490848294259147 112-113 0.002415141382376524 114-115 0.0030031758059116784 116-117 0.0021421254000209174 118-119 0.04553066536515048 120-121 0.012516732729533986 122-123 0.004851283994165019 124-125 0.0027511610529680408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2380813.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.89377410648913 #Duplication Level Percentage of deduplicated Percentage of total 1 81.70998614686822 52.20759397112354 2 10.31034820939991 13.17534118901285 3 3.4349232906820166 6.584106384238653 4 1.5735388661401377 4.0215734744375595 5 0.8381062347256205 2.677488521939948 6 0.4875353270802663 1.86902832344399 7 0.3357352773734192 1.50159757804537 8 0.22253553544734103 1.137490818603121 9 0.15334439684874987 0.8817977027474864 >10 0.8354451421392289 9.716811149989468 >50 0.07038686461467737 3.1612870948160774 >100 0.027950295207345405 3.005690157015032 >500 1.644134730966096E-4 0.060193634586862124 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.40049176478791E-5 2 0.0 0.0 0.0 0.0 8.40049176478791E-5 3 0.0 0.0 0.0 0.0 8.40049176478791E-5 4 0.0 0.0 0.0 0.0 8.40049176478791E-5 5 4.200245882393955E-5 0.0 0.0 0.0 8.40049176478791E-5 6 4.200245882393955E-5 0.0 0.0 0.0 8.40049176478791E-5 7 8.40049176478791E-5 0.0 0.0 0.0 8.40049176478791E-5 8 1.2600737647181867E-4 0.0 0.0 0.0 8.40049176478791E-5 9 1.2600737647181867E-4 0.0 0.0 0.0 8.40049176478791E-5 10-11 1.2600737647181867E-4 0.0 0.0 0.0 8.40049176478791E-5 12-13 1.680098352957582E-4 0.0 0.0 0.0 1.2600737647181867E-4 14-15 1.680098352957582E-4 0.0 0.0 0.0 1.4700860588378844E-4 16-17 1.680098352957582E-4 0.0 0.0 0.0 2.1001229411969776E-4 18-19 1.680098352957582E-4 0.0 0.0 2.1001229411969775E-5 2.1001229411969776E-4 20-21 1.680098352957582E-4 0.0 0.0 8.40049176478791E-5 2.3101352353166755E-4 22-23 1.680098352957582E-4 0.0 0.0 1.2600737647181867E-4 2.5201475294363735E-4 24-25 1.680098352957582E-4 0.0 0.0 1.680098352957582E-4 2.940172117675769E-4 26-27 1.680098352957582E-4 0.0 0.0 5.040295058872747E-4 3.360196705915164E-4 28-29 1.680098352957582E-4 0.0 0.0 0.002520147529436373 3.360196705915164E-4 30-31 1.680098352957582E-4 0.0 0.0 0.012474730270710046 3.360196705915164E-4 32-33 1.89011064707728E-4 0.0 0.0 0.036206119506235895 4.200245882393955E-4 34-35 2.3101352353166755E-4 0.0 0.0 0.06985008902421147 4.200245882393955E-4 36-37 3.1501844117954664E-4 0.0 0.0 0.12079907157765016 4.200245882393955E-4 38-39 3.360196705915164E-4 0.0 0.0 0.20249385399021258 4.200245882393955E-4 40-41 3.360196705915164E-4 0.0 0.0 0.299477531414689 4.200245882393955E-4 42-43 3.360196705915164E-4 0.0 0.0 0.39534814368033105 4.200245882393955E-4 44-45 3.9902335882742576E-4 0.0 0.0 0.5133960542050131 4.620270470633351E-4 46-47 5.880344235351538E-4 0.0 0.0 0.6522351818475454 5.040295058872747E-4 48-49 5.880344235351538E-4 0.0 0.0 0.79771069798426 5.460319647112142E-4 50-51 5.880344235351538E-4 0.0 0.0 0.9593151583093675 5.460319647112142E-4 52-53 5.880344235351538E-4 0.0 0.0 1.1182104600403306 6.720393411830328E-4 54-55 5.880344235351538E-4 0.0 0.0 1.295334828900884 6.720393411830328E-4 56-57 6.300368823590933E-4 0.0 0.0 1.4996557898499376 6.720393411830328E-4 58-59 6.300368823590933E-4 0.0 0.0 1.7213027650638668 6.720393411830328E-4 60-61 6.300368823590933E-4 0.0 0.0 1.9629009082191673 6.720393411830328E-4 62-63 6.300368823590933E-4 0.0 0.0 2.2004458140979573 6.720393411830328E-4 64-65 6.300368823590933E-4 0.0 0.0 2.448134313782729 6.720393411830328E-4 66-67 6.720393411830328E-4 0.0 0.0 2.731713914532557 6.720393411830328E-4 68-69 6.720393411830328E-4 0.0 0.0 3.054775826576888 6.720393411830328E-4 70-71 6.720393411830328E-4 0.0 0.0 3.4015481266273326 6.720393411830328E-4 72-73 6.720393411830328E-4 0.0 0.0 3.753423725424886 6.720393411830328E-4 74-75 6.720393411830328E-4 0.0 0.0 4.110150608216605 6.930405705950027E-4 76-77 6.720393411830328E-4 0.0 0.0 4.492078966302687 7.140418000069725E-4 78-79 6.720393411830328E-4 0.0 0.0 4.9156737635421175 7.140418000069725E-4 80-81 7.140418000069725E-4 0.0 0.0 5.376860761428974 7.770454882428818E-4 82-83 7.140418000069725E-4 0.0 0.0 5.856654848574836 8.40049176478791E-4 84-85 7.140418000069725E-4 0.0 0.0 6.33285772549125 8.40049176478791E-4 86-87 7.140418000069725E-4 0.0 0.0 6.824118483896047 8.610504058907608E-4 88-89 7.140418000069725E-4 0.0 0.0 7.3600908597189285 8.820516353027306E-4 90-91 7.140418000069725E-4 0.0 0.0 7.9380026906775125 8.820516353027306E-4 92-93 7.56044258830912E-4 0.0 0.0 8.53481562810687 8.820516353027306E-4 94-95 7.770454882428818E-4 0.0 0.0 9.141121121230437 8.820516353027306E-4 96-97 7.980467176548515E-4 0.0 0.0 9.74492746805398 9.4505532353864E-4 98-99 8.40049176478791E-4 0.0 0.0 10.380613681124892 9.660565529506097E-4 100-101 9.030528647147003E-4 0.0 0.0 11.071218109108107 9.660565529506097E-4 102-103 9.870577823625796E-4 0.0 0.0 11.798658693479917 9.660565529506097E-4 104-105 0.001134066388246368 0.0 0.0 12.522067041804627 9.660565529506097E-4 106-107 0.001218071305894247 0.0 0.0 13.248835586835252 9.660565529506097E-4 108-109 0.001218071305894247 0.0 0.0 14.006412935413238 0.0010080590117745494 110-111 0.0012810749941301563 0.0 0.0 14.795135107209177 0.0010080590117745494 112-113 0.001302076223542126 0.0 0.0 15.618761322287806 0.0010080590117745494 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2615 0.0 45.99325 1 ATCAACG 2830 0.0 41.85909 3 GGTATCA 1865 0.0 40.86444 1 CAACGCA 2920 0.0 40.568913 5 GTACATG 7245 0.0 38.70766 1 TCAACGC 3060 0.0 38.518284 4 AACGCAG 3125 0.0 37.706802 6 TACATGG 7710 0.0 36.2883 2 TATCAAC 3415 0.0 34.165554 2 ACATGGG 7975 0.0 33.81354 3 ACGCAGA 3775 0.0 31.520926 7 CATGGGG 5055 0.0 30.735657 4 CGCAGAG 3855 0.0 30.710527 8 CGTATAG 220 5.124093E-9 27.063986 1 ATGGGGA 3200 0.0 23.0672 5 CATGGGA 3670 0.0 21.897314 4 GCAGAGT 5550 0.0 20.7954 9 GAGTACA 5845 0.0 20.780695 1 AGTACAT 5735 0.0 19.82545 2 GGACCGA 705 0.0 19.41526 6 >>END_MODULE