FastQCFastQC Report
Thu 26 May 2016
SRR1364355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2380813
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.059.4630931
GTATCAA40550.052.4552731
ATCAACG49150.042.0461273
CAACGCA51000.040.754475
TCAACGC51250.040.3232574
AACGCAG53500.039.0390476
TATCAAC56050.037.295082
ACGCAGA63900.032.5880477
GTACATG68450.032.554421
CGCAGAG65050.031.9204678
TACATGG71900.030.3160212
ACATGGG72450.029.2638633
GCAGAGT83750.024.5089879
CATGGGG47500.024.4489964
TATCACG2001.8125265E-623.8220982
GAGTACT43300.022.60315912-13
CAGAGTA77500.022.493510-11
AGAGTAC74650.021.95750210-11
GTACTTT48200.019.93501714-15
CCCTATA5650.018.9733522
Produced by FastQC (version 0.11.5)