FastQCFastQC Report
Thu 26 May 2016
SRR1364354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878313
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13850.041.6846851
GTATCAA20800.040.9192121
ATCAACG21950.036.0570953
CAACGCA22300.035.4911775
GTACATG60550.035.3869321
TCAACGC22900.034.301424
TACATGG61600.034.2951622
ACATGGG60450.034.1591263
AACGCAG23750.033.323466
TATCAAC26550.030.0348472
GTATAGG6800.027.1335951
ACGCAGA29400.026.9144467
CATGGGG35050.025.9762844
CGCAGAG30750.025.9263158
CATGGGA32250.025.4637854
GAGTACA44150.024.6703281
TAAGGTG11350.023.0690885
TATAGGG6450.022.1430132
TAAGGTA7150.020.8069084
ATGGGAT17100.020.5319185
Produced by FastQC (version 0.11.5)