##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364354_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1878313 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.428209249470136 31.0 31.0 34.0 26.0 34.0 2 30.55098484650854 31.0 31.0 34.0 26.0 34.0 3 30.635930752755264 31.0 31.0 34.0 26.0 34.0 4 34.07624022194384 37.0 35.0 37.0 30.0 37.0 5 34.09665055824029 37.0 35.0 37.0 30.0 37.0 6 34.19533964786486 37.0 35.0 37.0 30.0 37.0 7 34.11413167028073 37.0 35.0 37.0 30.0 37.0 8 34.02412430729064 37.0 35.0 37.0 30.0 37.0 9 35.510911120776996 39.0 37.0 39.0 30.0 39.0 10-11 35.41669519403848 39.0 36.0 39.0 29.0 39.0 12-13 35.400994935348905 39.0 35.0 39.0 29.0 39.0 14-15 36.63047399448335 40.0 37.0 41.0 29.0 41.0 16-17 36.6160946019114 40.0 37.0 41.0 29.0 41.0 18-19 36.61128443448989 40.0 37.0 41.0 29.0 41.0 20-21 36.619135628619944 40.0 37.0 41.0 29.0 41.0 22-23 36.57551510318035 40.0 37.0 41.0 28.5 41.0 24-25 36.5676090726093 40.0 37.0 41.0 29.0 41.0 26-27 36.497642299233405 40.0 37.0 41.0 29.0 41.0 28-29 36.49214321574732 40.0 37.0 41.0 29.0 41.0 30-31 36.384418358388615 40.0 36.0 41.0 28.5 41.0 32-33 36.33410326180993 40.0 36.0 41.0 29.0 41.0 34-35 36.321905081847376 40.0 36.0 41.0 29.0 41.0 36-37 36.24946294893343 40.0 36.0 41.0 29.0 41.0 38-39 36.14441256595679 40.0 36.0 41.0 28.0 41.0 40-41 36.07962703766624 40.0 36.0 41.0 28.0 41.0 42-43 35.94002197716781 40.0 35.5 41.0 27.0 41.0 44-45 35.804633200110956 40.0 35.0 41.0 27.0 41.0 46-47 35.68425842764225 40.0 35.0 41.0 27.0 41.0 48-49 35.52767776190656 39.5 35.0 41.0 27.0 41.0 50-51 35.39537020720189 39.0 35.0 41.0 26.5 41.0 52-53 35.22578824721971 39.0 35.0 41.0 26.0 41.0 54-55 35.038838308631206 39.0 34.0 41.0 26.0 41.0 56-57 34.84274479280077 39.0 34.0 40.5 26.0 41.0 58-59 34.6150572348698 38.0 34.0 40.0 25.0 41.0 60-61 34.36313729394409 38.0 34.0 40.0 25.0 41.0 62-63 34.09702908940096 37.5 33.5 40.0 24.0 41.0 64-65 33.76112554190915 37.0 33.0 40.0 23.0 41.0 66-67 33.45675028602794 36.5 33.0 39.5 22.5 41.0 68-69 33.132805874207335 36.0 33.0 39.0 22.0 41.0 70-71 32.77673289808461 35.5 32.0 39.0 22.0 40.5 72-73 32.43604739998072 35.0 32.0 38.0 21.0 40.0 74-75 32.089279316067135 35.0 32.0 37.0 20.0 39.5 76-77 31.865571393053234 35.0 32.0 37.0 20.0 39.0 78-79 31.708287170455616 35.0 32.0 36.5 20.0 39.0 80-81 31.454921517340296 35.0 32.0 36.0 20.0 38.5 82-83 31.162532282958164 35.0 32.0 36.0 18.5 37.0 84-85 30.923021615673214 35.0 32.0 35.0 18.0 37.0 86-87 30.727525178178503 35.0 31.5 35.0 18.0 36.5 88-89 30.47363698169581 35.0 31.0 35.0 14.5 36.0 90-91 30.351317112749577 35.0 31.0 35.0 12.5 36.0 92-93 30.21766659763309 35.0 31.0 35.0 10.5 36.0 94-95 30.116389547428994 35.0 31.0 35.0 8.5 35.0 96-97 30.019432597229535 35.0 31.0 35.0 7.0 35.0 98-99 29.896099851302736 34.0 31.0 35.0 7.0 35.0 100-101 29.81224668093124 34.0 31.0 35.0 4.0 35.0 102-103 28.804976060965345 33.5 29.0 34.5 2.0 35.0 104-105 29.523215779265755 34.0 30.0 35.0 2.0 35.0 106-107 29.878758492327954 34.5 31.0 35.0 2.0 35.0 108-109 29.935021213184385 35.0 31.0 35.0 2.0 35.0 110-111 29.87326313559029 35.0 31.0 35.0 2.0 35.0 112-113 29.790647778085976 35.0 31.0 35.0 2.0 35.0 114-115 29.700149282893747 35.0 31.0 35.0 2.0 35.0 116-117 29.585895428504195 35.0 31.0 35.0 2.0 35.0 118-119 29.42255337635421 35.0 31.0 35.0 2.0 35.0 120-121 29.25003500481549 35.0 31.0 35.0 2.0 35.0 122-123 29.085784158444305 35.0 31.0 35.0 2.0 35.0 124-125 28.274213083761865 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3081887593432633 1101 2 0.34362073157964446 1101 3 0.33261423163746784 1101 4 0.42477766816428897 1101 5 0.3242886041663837 1101 6 0.38718141601997047 1101 7 0.4296629956532456 1101 8 0.309798753141024 1101 9 0.4831287975495897 1101 10-11 0.5969442266551326 1101 12-13 0.6615964988626075 1101 14-15 0.6729755028503988 1101 16-17 0.5895762501583945 1101 18-19 0.4675554167593958 1101 20-21 0.5112449774601728 1101 22-23 0.6748212165117167 1101 24-25 0.7288203793381314 1101 26-27 0.7786561811401924 1101 28-29 0.690004295651157 1101 30-31 0.7948792854625921 1101 32-33 0.6716572772941589 1101 34-35 0.6968173984726675 1101 36-37 0.5450932140677764 1101 38-39 0.7847333111222099 1101 40-41 0.667922659666587 1101 42-43 0.8619488526816141 1101 44-45 0.7088527019663644 1101 46-47 0.6664581632187492 1101 48-49 0.5129828989479606 1101 50-51 0.5177717347832171 1101 52-53 0.46777012763826065 1101 54-55 0.46672350662426965 1101 56-57 0.36931134592164483 1101 58-59 0.4542760656767939 1101 60-61 0.5133875033471895 1101 62-63 0.46620219199322577 1101 64-65 0.647676937238856 1101 66-67 0.4640574130989066 1101 68-69 0.49187743128099726 1101 70-71 0.4510827813423859 1101 72-73 0.3285321811720081 1101 74-75 0.21535181846090268 1101 76-77 0.43477578903922875 1101 78-79 0.38335914396054704 1101 80-81 0.44199852072944523 1101 82-83 0.3898990528279782 1101 84-85 0.34401021826278466 1101 86-87 0.36232811471092674 1101 88-89 0.39795897307541495 1101 90-91 0.344712456951239 1101 92-93 0.39699997643278806 1101 94-95 0.46449641851528867 1101 96-97 0.36135862326716506 1101 98-99 0.41831023932135025 1101 100-101 0.43480605863843635 1101 102-103 0.17209438068801575 1101 104-105 0.3027637079851928 1101 106-107 0.35695873718698934 1101 108-109 0.3598476322875612 1101 110-111 0.4212586749317069 1101 112-113 0.37112604379055014 1101 114-115 0.3274157996904563 1101 116-117 0.2557593282220587 1101 118-119 0.41613913434192185 1101 120-121 0.32990848362763003 1101 122-123 0.28683196717635795 1101 124-125 0.09298288646446906 1102 1 0.36806789868887435 1102 2 0.3650747498339548 1102 3 0.4328639927498621 1102 4 0.3969399140372971 1102 5 0.4133234982320886 1102 6 0.46800503459059684 1102 7 0.36674467873999816 1102 8 0.4483671408967993 1102 9 0.5820770330762954 1102 10-11 0.505183575562846 1102 12-13 0.5041979684332674 1102 14-15 0.5907181632345981 1102 16-17 0.573374134767441 1102 18-19 0.6420468332805314 1102 20-21 0.6416708234517969 1102 22-23 0.6401382945043963 1102 24-25 0.662843048948325 1102 26-27 0.6557074843132824 1102 28-29 0.5615147902308735 1102 30-31 0.6196156523939536 1102 32-33 0.656395818926498 1102 34-35 0.5992059450475082 1102 36-37 0.41949138204694236 1102 38-39 0.453185978425914 1102 40-41 0.461623539431983 1102 42-43 0.3554687709655795 1102 44-45 0.3433993427123525 1102 46-47 0.302477537827464 1102 48-49 0.39326139247328484 1102 50-51 0.2925364387273319 1102 52-53 0.3196294310749437 1102 54-55 0.3381478039651995 1102 56-57 0.33324953169383775 1102 58-59 0.23227632927690678 1102 60-61 0.27511559982482936 1102 62-63 0.28271173102251623 1102 64-65 0.2503953793621605 1102 66-67 0.27992192263793214 1102 68-69 0.1900597766630483 1102 70-71 0.15018897924017693 1102 72-73 0.21970539939995604 1102 74-75 0.20790392890935294 1102 76-77 0.3104134706761883 1102 78-79 0.45995231012747695 1102 80-81 0.4404823587471718 1102 82-83 0.420873384766427 1102 84-85 0.44384131648677894 1102 86-87 0.4476082227869753 1102 88-89 0.52143052073761 1102 90-91 0.42195454071017835 1102 92-93 0.4412532302467511 1102 94-95 0.448995874840044 1102 96-97 0.34936791517134935 1102 98-99 0.3732283585345044 1102 100-101 0.3211369426873354 1102 102-103 0.1589729598833749 1102 104-105 0.3480395325593122 1102 106-107 0.2968494749378223 1102 108-109 0.35287521144997314 1102 110-111 0.3251520486837265 1102 112-113 0.3120759927180252 1102 114-115 0.24865472024797342 1102 116-117 0.2989399272533291 1102 118-119 0.30985807071544613 1102 120-121 0.2758885818186769 1102 122-123 0.2482768253265455 1102 124-125 0.13166310657056712 1103 1 0.5117444299118574 1103 2 0.6344832754707177 1103 3 0.6135689205945098 1103 4 0.6458275911005416 1103 5 0.5414368187082488 1103 6 0.5534670283393481 1103 7 0.5415204983848767 1103 8 0.5309064772005812 1103 9 0.6500267570903944 1103 10-11 0.8352176249077914 1103 12-13 0.814601905388777 1103 14-15 0.8521109942514258 1103 16-17 0.7868697788453858 1103 18-19 0.6929998159233506 1103 20-21 0.8608254420592374 1103 22-23 0.9615418989467699 1103 24-25 0.8813871734412402 1103 26-27 0.9586641911032032 1103 28-29 0.9674710315024129 1103 30-31 1.011681090978783 1103 32-33 1.0144100504886495 1103 34-35 1.0348533481199311 1103 36-37 0.9292220464081922 1103 38-39 0.8704693461189379 1103 40-41 0.8901422599214399 1103 42-43 0.8269977993622035 1103 44-45 0.8191339972312122 1103 46-47 0.7984923014106258 1103 48-49 0.804584395731915 1103 50-51 0.5997059539231842 1103 52-53 0.7737250618523603 1103 54-55 0.7135299945617319 1103 56-57 0.7091370092491971 1103 58-59 0.7035046327949033 1103 60-61 0.75096397383561 1103 62-63 0.778241158434902 1103 64-65 0.7880042109110121 1103 66-67 0.6928709151231303 1103 68-69 0.5676937462048528 1103 70-71 0.7035232879453659 1103 72-73 0.7196017883073154 1103 74-75 0.5688614337307953 1103 76-77 0.6529376443810477 1103 78-79 0.7927422283292458 1103 80-81 0.7626830636750164 1103 82-83 0.6567236208507516 1103 84-85 0.5413965469183815 1103 86-87 0.5194039267172812 1103 88-89 0.5566872873798907 1103 90-91 0.5220835558062724 1103 92-93 0.508899968292905 1103 94-95 0.49066229766374647 1103 96-97 0.5261005081087688 1103 98-99 0.4799464538992737 1103 100-101 0.4462564479077926 1103 102-103 0.18265693940056948 1103 104-105 0.3640175866924551 1103 106-107 0.36508635729467187 1103 108-109 0.4121375643129106 1103 110-111 0.4092359749988148 1103 112-113 0.4155993018887365 1103 114-115 0.4004742321186896 1103 116-117 0.35895229674192564 1103 118-119 0.3164958309069732 1103 120-121 0.05786636994130845 1103 122-123 0.12196705609066072 1103 124-125 0.021100627352637957 1104 1 0.42377626952595904 1104 2 0.4768165525856567 1104 3 0.47773476554142746 1104 4 0.4530353235782556 1104 5 0.5125892819533178 1104 6 0.45742023325332326 1104 7 0.4586096652386473 1104 8 0.48395592834697254 1104 9 0.6384239534826222 1104 10-11 0.6536228194872322 1104 12-13 0.5503821011322501 1104 14-15 0.7020038775203119 1104 16-17 0.6810170562024425 1104 18-19 0.6719304645007966 1104 20-21 0.6864648314240256 1104 22-23 0.6655325339283422 1104 24-25 0.6591087040852699 1104 26-27 0.6765233516142999 1104 28-29 0.7314895877106267 1104 30-31 0.649832702670416 1104 32-33 0.663617112484431 1104 34-35 0.7015821826712738 1104 36-37 0.645024363916562 1104 38-39 0.5012430698493375 1104 40-41 0.5163337104490822 1104 42-43 0.5189343746688095 1104 44-45 0.4793530523690279 1104 46-47 0.5816248954203687 1104 48-49 0.40282086270602235 1104 50-51 0.36373265834928503 1104 52-53 0.37446497177187155 1104 54-55 0.4501526901681032 1104 56-57 0.2996401707577405 1104 58-59 0.3981095554566778 1104 60-61 0.24891295098852595 1104 62-63 0.2722219534733341 1104 64-65 0.44324017812775196 1104 66-67 0.3329364526623593 1104 68-69 0.3507254575168517 1104 70-71 0.1338424445867119 1104 72-73 0.24452628148816302 1104 74-75 0.12967041984416028 1104 76-77 0.18965752365291166 1104 78-79 0.3294909497057148 1104 80-81 0.2604093228721567 1104 82-83 0.23789215807232722 1104 84-85 0.2702458426536829 1104 86-87 0.3322754466522184 1104 88-89 0.38952245850281386 1104 90-91 0.2697994680600537 1104 92-93 0.2943339811789869 1104 94-95 0.2431868225062388 1104 96-97 0.28455667690466413 1104 98-99 0.23448526993993113 1104 100-101 0.26484749945729646 1104 102-103 0.06052317204744995 1104 104-105 0.12959115915054298 1104 106-107 0.16635416826429505 1104 108-109 0.2343768187535602 1104 110-111 0.27949112544854415 1104 112-113 0.3034004108741293 1104 114-115 0.2737428004845661 1104 116-117 0.2606894450622548 1104 118-119 0.20471495640401471 1104 120-121 0.10256982137120119 1104 122-123 0.046451571372578826 1104 124-125 -0.04262813683948963 1105 1 0.27819251708920945 1105 2 0.34884839294587877 1105 3 0.2394490372564313 1105 4 0.34397831501419773 1105 5 0.39042044864823566 1105 6 0.3737669163991413 1105 7 0.2986302791770328 1105 8 0.412724221779861 1105 9 0.44536377108201464 1105 10-11 0.4303728168623664 1105 12-13 0.5834462733273824 1105 14-15 0.6202296513929539 1105 16-17 0.572389463023498 1105 18-19 0.5253065781309445 1105 20-21 0.5511501126320439 1105 22-23 0.5496871874636824 1105 24-25 0.48953818696689666 1105 26-27 0.5610537357995184 1105 28-29 0.5603247776983409 1105 30-31 0.5702906740848874 1105 32-33 0.633219302971618 1105 34-35 0.532966991353625 1105 36-37 0.4451699064211283 1105 38-39 0.477689657850064 1105 40-41 0.517715701776531 1105 42-43 0.5594240394510663 1105 44-45 0.46741566714437255 1105 46-47 0.6119343969810345 1105 48-49 0.47107134780603843 1105 50-51 0.448127749502099 1105 52-53 0.5197156163636762 1105 54-55 0.5388857454157616 1105 56-57 0.4174791020527806 1105 58-59 0.40111433821035547 1105 60-61 0.36800206789899903 1105 62-63 0.4995635578807054 1105 64-65 0.44565030400627137 1105 66-67 0.35935146728910894 1105 68-69 0.32970385262949975 1105 70-71 0.3116757236728418 1105 72-73 0.22449681300579982 1105 74-75 0.205246902655297 1105 76-77 0.19812939985128963 1105 78-79 0.263560845106106 1105 80-81 0.3161928250217976 1105 82-83 0.4099077367700268 1105 84-85 0.301828591142133 1105 86-87 0.23099310019306074 1105 88-89 0.26939870737599847 1105 90-91 0.26605878570519437 1105 92-93 0.32721672521767076 1105 94-95 0.2814264156671946 1105 96-97 0.2551260050842643 1105 98-99 0.29065468971481323 1105 100-101 0.2201171155720658 1105 102-103 0.18748550848712497 1105 104-105 0.3064714873136438 1105 106-107 0.3667789713869354 1105 108-109 0.3511555546486598 1105 110-111 0.23173452241360337 1105 112-113 0.33943580505132687 1105 114-115 0.3319131797100354 1105 116-117 0.25563108056802264 1105 118-119 0.3856652624632524 1105 120-121 0.327270374292727 1105 122-123 0.2968616127554462 1105 124-125 0.27102214767085897 1106 1 0.3033741455146739 1106 2 0.41264432830018194 1106 3 0.3132893355634714 1106 4 0.3822245763024057 1106 5 0.4200045546058817 1106 6 0.24073433880593598 1106 7 0.26344884329053286 1106 8 0.22050553602122847 1106 9 0.42822101479290353 1106 10-11 0.4625707010072304 1106 12-13 0.35690708473595834 1106 14-15 0.5240310123966054 1106 16-17 0.4697500870274709 1106 18-19 0.3839545275415759 1106 20-21 0.31792704488399437 1106 22-23 0.3713099552458914 1106 24-25 0.384409072310433 1106 26-27 0.4639229490161796 1106 28-29 0.5587909456397142 1106 30-31 0.4833248956646514 1106 32-33 0.4986958443201033 1106 34-35 0.4996109729804701 1106 36-37 0.45404064563252433 1106 38-39 0.4114128702491726 1106 40-41 0.4249396897620983 1106 42-43 0.38420302693307207 1106 44-45 0.32388829649600126 1106 46-47 0.40454419602959746 1106 48-49 0.44049628328080104 1106 50-51 0.30424720561407526 1106 52-53 0.3232801420957685 1106 54-55 0.38239056678897043 1106 56-57 0.44233853626468544 1106 58-59 0.4312866136852378 1106 60-61 0.2529325119629746 1106 62-63 0.2882409336634595 1106 64-65 0.49577399795028043 1106 66-67 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-2.199705521507582 2216 98-99 -2.2397550886541424 2216 100-101 -2.057935968505518 2216 102-103 -2.02440709792009 2216 104-105 -2.1654474616603387 2216 106-107 -2.0522535203328047 2216 108-109 -1.9908842472993697 2216 110-111 -1.905044351736663 2216 112-113 -2.052866860671358 2216 114-115 -2.0368930989848195 2216 116-117 -2.1200523218270675 2216 118-119 -2.1675772944973772 2216 120-121 -2.051935905832398 2216 122-123 -2.076055807358692 2216 124-125 -1.9311755692045232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 49596.0 3 8673.0 4 5339.0 5 4296.0 6 4518.0 7 5007.0 8 5670.0 9 6409.0 10 7118.0 11 7874.0 12 8306.0 13 8279.0 14 8169.0 15 7678.0 16 6971.0 17 6380.0 18 6094.0 19 5968.0 20 5786.0 21 6335.0 22 7283.0 23 9601.0 24 13571.0 25 18167.0 26 21631.0 27 22972.0 28 26630.0 29 32074.0 30 38176.0 31 46637.0 32 59207.0 33 77200.0 34 106808.0 35 160911.0 36 308039.0 37 537620.0 38 216788.0 39 532.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.876995578223685 18.512073867419147 12.666402489233173 24.944528065123997 2 15.644089626637319 19.756600521684405 39.533540239395535 25.065769612282747 3 18.65222354259039 24.248880881494827 29.287469706069007 27.811425869845774 4 12.281102469946264 16.1726081995763 37.62953095459049 33.91675837588694 5 13.24353713385612 38.31763657141396 34.97408250358649 13.464743791143427 6 32.80870884112894 36.78543811550579 17.306963110659503 13.098889932705765 7 28.76187940902436 32.085611606548646 21.444642619459604 17.70786636496739 8 26.22699459993004 35.38076003469078 20.183633257360743 18.208612108018436 9 26.282360820587407 15.963473606369119 19.74937084500826 38.004794728035215 10-11 24.72920492687182 26.55653761370252 28.23972488056427 20.47453257886139 12-13 25.27879635680716 23.919267315214977 27.851551737677337 22.950384590300523 14-15 22.24864379090622 25.705882243330493 25.000139753623678 27.04533421213961 16-17 22.686824143292604 28.64946982940254 26.23421420473346 22.4294918225714 18-19 22.76226427952141 27.704897566190468 27.19712741849657 22.335710735791555 20-21 22.98899224410002 27.19745175455409 27.232909122659066 22.58064687868682 22-23 23.00063940354989 27.02829613594752 27.05472943007901 22.916335030423575 24-25 22.799022314172348 27.241040231313953 27.081562018683787 22.878375435829916 26-27 22.50133830375755 27.46292479331825 26.85730074979616 23.178436153128036 28-29 22.75358993306742 27.636202369366476 26.832473694410076 22.777734003156034 30-31 22.66031449581219 27.210127412543628 26.849377205442345 23.280180886201837 32-33 22.346062960370993 27.383281287392354 26.84740020076574 23.423255551470913 34-35 23.245866806651442 27.4083552702857 26.51975896282781 22.826018960235043 36-37 23.358405503667925 26.86269739143305 26.56705944862166 23.211837656277368 38-39 22.730809518675724 27.262043089323768 26.744529316951564 23.262618075048948 40-41 23.05289357397647 26.964994942235 26.989405313315235 22.9927061704733 42-43 22.85212463623983 26.913514255991327 26.782571242688856 23.451789865079984 44-45 22.80644778306214 27.21822893069171 27.065243953406533 22.910079332839622 46-47 23.289294667045375 27.498846703541986 26.28419194579813 22.92766668361451 48-49 22.91420545989939 27.367643198976953 26.4760186401308 23.242132700992858 50-51 22.92551880330914 27.42972017975705 26.684104299975566 22.96065671695825 52-53 23.224028587613454 27.098204968758914 26.682642139471096 22.995124304156533 54-55 22.604196559249473 27.250082922801134 26.930770242347545 23.21495027560185 56-57 22.72550251941838 27.637443330056456 26.68328798673063 22.953766163794537 58-59 22.72195359841729 27.037056952624134 26.80226992734912 23.438719521609457 60-61 22.28741430728019 27.251729027727535 27.199341104740977 23.261515560251304 62-63 22.51774677986486 27.230602590589303 27.308199415588096 22.94345121395774 64-65 22.40969545057907 27.332489300068204 26.84528902194185 23.412526227410886 66-67 22.23565659195418 27.536420540849623 26.821399726449968 23.40652314074623 68-69 22.758675215745697 26.97472065797313 27.25593942882029 23.010664697460886 70-71 22.537771299030283 26.888644811896857 26.949817865087063 23.6237660239858 72-73 22.39869432590262 27.070908847454046 26.912517865571044 23.61787896107229 74-75 22.491740402535417 27.382545476035254 26.817264508634143 23.308449612795183 76-77 22.693658963024326 27.039582300700264 27.01400050790906 23.25275822836635 78-79 22.661879983484774 27.095409517233044 27.020074094916772 23.222636404365407 80-81 22.868226649832202 27.157528165919043 26.831273879771995 23.14297130447676 82-83 22.609184993049578 27.23005819614833 27.016115078344654 23.144641732457437 84-85 22.570642982845396 27.295898606213438 27.036994346751225 23.09646406418994 86-87 22.745858436140377 27.13184856751617 26.95639511978005 23.165897876563402 88-89 22.58310718680103 27.294116143747733 26.811528228784155 23.311248440667082 90-91 22.513090259569132 27.400289090429936 27.01249264629546 23.074128003705468 92-93 22.686006953132903 26.81508180312944 27.254737021439713 23.244174222297943 94-95 22.254003434500518 26.738180161939546 27.49189623453417 23.515920169025765 96-97 22.649889128762368 26.39685780530851 27.517948363018785 23.435304702910337 98-99 22.30314359063365 26.753901670554935 27.11484013718803 23.828114601623383 100-101 22.271726601660557 26.628839606343018 27.524417221028756 23.575016570967662 102-103 22.0852799229084 26.30428822037124 27.700136028345007 23.910295828375354 104-105 21.953645812952523 26.634770777914156 27.38241552128163 24.029167887851692 106-107 22.1375187880653 26.64143666205761 27.126745100461243 24.094299449415846 108-109 22.21353517800366 26.465146762243673 27.152559048829882 24.168759010922784 110-111 22.155784582326653 26.740443148700464 27.289188121510783 23.8145841474621 112-113 22.18983717707941 26.742836150867756 27.582892213169853 23.484434458882987 114-115 22.060494351681186 27.096064544913425 27.19381469425393 23.64962640915146 116-117 21.894998392142583 26.949367398614477 27.42746539912132 23.728168810121623 118-119 21.822400312765154 27.125372913323254 27.119726948350603 23.93249982556099 120-121 21.701987633002727 27.298551752523743 27.27714671978523 23.722313894688305 122-123 21.821356476442006 27.288455359350962 27.28635231160885 23.60383585259818 124-125 21.680038972240265 27.225676956331924 27.36048257429749 23.733801497130322 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 259.0 1 240.5 2 217.0 3 186.5 4 167.0 5 146.0 6 122.0 7 111.5 8 106.0 9 105.5 10 104.0 11 110.5 12 112.0 13 108.5 14 135.5 15 175.0 16 198.0 17 238.5 18 328.0 19 431.5 20 585.5 21 848.5 22 1287.0 23 1920.0 24 2783.5 25 4171.0 26 6268.0 27 8894.5 28 11792.5 29 15370.0 30 19245.0 31 24152.5 32 31482.5 33 35027.0 34 35866.0 35 39759.5 36 46012.5 37 51577.5 38 55179.5 39 58612.0 40 60384.0 41 62538.0 42 64061.5 43 64608.5 44 66503.5 45 70925.0 46 72396.5 47 70609.0 48 68735.5 49 66442.0 50 66176.5 51 65493.0 52 65411.0 53 67246.0 54 69581.5 55 66459.0 56 59415.0 57 54481.5 58 51040.0 59 43982.5 60 34143.5 61 28005.5 62 23760.0 63 17957.0 64 12321.5 65 8752.5 66 6229.5 67 4772.0 68 3556.5 69 2441.5 70 1746.0 71 1163.0 72 806.0 73 549.0 74 401.0 75 315.5 76 215.0 77 122.5 78 66.0 79 40.5 80 33.5 81 24.0 82 15.5 83 11.0 84 11.0 85 9.5 86 7.5 87 4.5 88 4.5 89 6.0 90 3.0 91 3.5 92 4.0 93 3.5 94 2.5 95 1.0 96 1.5 97 1.0 98 1.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04189930006340797 2 0.008837717675382111 3 0.0026087238921308643 4 0.005004490731842882 5 0.022946122398130663 6 0.03135792596867508 7 0.003354073575596826 8 2.1295705241884607E-4 9 0.0 10-11 7.985889465706728E-5 12-13 8.252085781230285E-4 14-15 3.9929447328533634E-4 16-17 1.330981577617788E-4 18-19 1.330981577617788E-4 20-21 5.3239263104711516E-5 22-23 0.0 24-25 0.0 26-27 1.330981577617788E-4 28-29 2.661963155235576E-4 30-31 2.661963155235576E-4 32-33 2.395766839712018E-4 34-35 7.187300519136055E-4 36-37 1.0647852620942303E-4 38-39 2.9281594707591333E-4 40-41 6.921104203612497E-4 42-43 7.985889465706727E-4 44-45 0.0012777423145130765 46-47 7.985889465706728E-5 48-49 0.0 50-51 0.0 52-53 9.050674727800957E-4 54-55 0.0031943557862826907 56-57 1.330981577617788E-4 58-59 7.985889465706727E-4 60-61 9.316871043324515E-4 62-63 7.719693150183169E-4 64-65 0.0015971778931413454 66-67 0.0024490061028167296 68-69 0.0031943557862826907 70-71 0.001863374208664903 72-73 0.00263534352368322 74-75 0.002315907945054951 76-77 0.0012777423145130765 78-79 0.0024756257343690855 80-81 0.0012511226829607204 82-83 5.323926310471152E-4 84-85 6.65490788808894E-4 86-87 0.001969852734874326 88-89 0.00564336188909942 90-91 4.2591410483769213E-4 92-93 0.0012245030514083648 94-95 0.0010381656305418745 96-97 4.2591410483769213E-4 98-99 7.985889465706727E-4 100-101 0.0014906993669319224 102-103 0.0014906993669319224 104-105 7.719693150183169E-4 106-107 0.006708147151193652 108-109 0.001969852734874326 110-111 0.001437460103827211 112-113 0.0024223864712643738 114-115 0.002928159470759133 116-117 0.001969852734874326 118-119 0.04610520184868017 120-121 0.01330981577617788 122-123 0.004605196258557546 124-125 0.00282168094454971 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1878313.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.56492186402443 #Duplication Level Percentage of deduplicated Percentage of total 1 86.1760417867115 60.810056552301994 2 8.68052046805428 12.2508049713463 3 2.356122638400321 4.987788296423331 4 0.9858645779965635 2.782698276593477 5 0.4871711245132742 1.7188596167844052 6 0.28643449834303714 1.2127336796842467 7 0.18314161360178646 0.9046361557702991 8 0.11631979801857213 0.6566477966735716 9 0.08984259944013946 0.5705762408598857 >10 0.5507509618604997 7.511992507632048 >50 0.05776821180632672 2.815402747717933 >100 0.02953223669018604 3.4578435492198794 >500 3.263230423389237E-4 0.14069472550881976 >1k 1.6316152116946185E-4 0.17926488348382352 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.3239263104711516E-5 0.0 0.0 0.0 0.0 8 5.3239263104711516E-5 0.0 0.0 0.0 0.0 9 5.3239263104711516E-5 0.0 0.0 0.0 0.0 10-11 5.3239263104711516E-5 0.0 0.0 5.3239263104711516E-5 0.0 12-13 1.0647852620942303E-4 0.0 0.0 5.3239263104711516E-5 0.0 14-15 2.1295705241884607E-4 0.0 0.0 5.3239263104711516E-5 5.3239263104711516E-5 16-17 2.1295705241884607E-4 0.0 0.0 5.3239263104711516E-5 1.0647852620942303E-4 18-19 2.3957668397120183E-4 0.0 0.0 5.3239263104711516E-5 1.0647852620942303E-4 20-21 2.661963155235576E-4 0.0 0.0 5.3239263104711516E-5 1.5971778931413454E-4 22-23 2.661963155235576E-4 0.0 0.0 1.0647852620942303E-4 1.5971778931413454E-4 24-25 2.661963155235576E-4 0.0 0.0 2.3957668397120183E-4 1.5971778931413454E-4 26-27 2.661963155235576E-4 0.0 0.0 6.65490788808894E-4 1.863374208664903E-4 28-29 6.388711572565381E-4 0.0 0.0 0.002315907945054951 2.1295705241884607E-4 30-31 6.921104203612497E-4 0.0 0.0 0.009929122569028698 2.1295705241884607E-4 32-33 7.187300519136055E-4 0.0 0.0 0.02653977265769869 2.661963155235576E-4 34-35 7.719693150183169E-4 0.0 0.0 0.050390962528609445 3.726748417329806E-4 36-37 8.518282096753843E-4 0.0 0.0 0.0922370233289127 3.726748417329806E-4 38-39 8.518282096753843E-4 2.6619631552355758E-5 0.0 0.16075595494467643 3.992944732853364E-4 40-41 8.518282096753843E-4 5.3239263104711516E-5 0.0 0.2345988128709113 4.5253373639004787E-4 42-43 8.518282096753843E-4 5.3239263104711516E-5 0.0 0.3086280082180126 5.057729994947595E-4 44-45 8.518282096753843E-4 5.3239263104711516E-5 0.0 0.39575406228887305 5.590122625994709E-4 46-47 9.050674727800957E-4 5.3239263104711516E-5 0.0 0.5059859565471783 6.65490788808894E-4 48-49 9.583067358848073E-4 5.3239263104711516E-5 0.0 0.6224468445887348 6.921104203612497E-4 50-51 9.583067358848073E-4 5.3239263104711516E-5 0.0 0.7522707876695738 6.921104203612497E-4 52-53 9.583067358848073E-4 5.3239263104711516E-5 0.0 0.878367982333083 7.719693150183169E-4 54-55 9.583067358848073E-4 5.3239263104711516E-5 0.0 1.0130366983564507 7.985889465706727E-4 56-57 0.0010115459989895187 5.3239263104711516E-5 0.0 1.1707313956726062 7.985889465706727E-4 58-59 0.0010115459989895187 5.3239263104711516E-5 0.0 1.3485239148107904 7.985889465706727E-4 60-61 0.0010115459989895187 5.3239263104711516E-5 0.0 1.5416493417231312 8.252085781230284E-4 62-63 0.0010647852620942304 5.3239263104711516E-5 0.0 1.7299566153244958 9.050674727800957E-4 64-65 0.0010647852620942304 5.3239263104711516E-5 0.0 1.9218841588169808 9.050674727800957E-4 66-67 0.0010647852620942304 5.3239263104711516E-5 0.0 2.1553649471626932 9.050674727800957E-4 68-69 0.0011180245251989419 5.3239263104711516E-5 0.0 2.4174086001640838 9.583067358848073E-4 70-71 0.0011712637883036533 5.3239263104711516E-5 0.0 2.6923095352052613 9.583067358848073E-4 72-73 0.0012511226829607204 5.3239263104711516E-5 0.0 2.979242543708104 0.0011446441567512975 74-75 0.0012777423145130763 5.3239263104711516E-5 0.0 3.2644452761600435 0.0012245030514083648 76-77 0.0012777423145130763 5.3239263104711516E-5 0.0 3.5732596217989228 0.0012245030514083648 78-79 0.0013043619460654321 5.3239263104711516E-5 0.0 3.9262093165516077 0.0012511226829607204 80-81 0.0015439386300366339 5.3239263104711516E-5 0.0 4.307003145908057 0.0012777423145130763 82-83 0.0015971778931413454 5.3239263104711516E-5 0.0 4.6973001837286965 0.0012777423145130763 84-85 0.0016770367877984127 5.3239263104711516E-5 0.0 5.093879454595694 0.0012777423145130763 86-87 0.0017835153140078356 5.3239263104711516E-5 0.0 5.502464179292801 0.0012777423145130763 88-89 0.0018101349455601915 5.3239263104711516E-5 0.0 5.939452050856274 0.001330981577617788 90-91 0.0019166134717696144 5.3239263104711516E-5 0.0 6.430238197787057 0.001330981577617788 92-93 0.001969852734874326 5.3239263104711516E-5 0.0 6.923393491925999 0.001330981577617788 94-95 0.001969852734874326 5.3239263104711516E-5 0.0 7.41798624616877 0.001330981577617788 96-97 0.001969852734874326 5.3239263104711516E-5 0.0 7.917876307090458 0.0014906993669319224 98-99 0.002076331261083749 5.3239263104711516E-5 0.0 8.43996714072681 0.0015971778931413454 100-101 0.0021828097872931725 5.3239263104711516E-5 0.0 9.025785372299506 0.0015971778931413454 102-103 0.0022360490503978837 5.3239263104711516E-5 0.0 9.63928802068665 0.0017036564193507685 104-105 0.002661963155235576 5.3239263104711516E-5 0.0 10.247280405342455 0.0017036564193507685 106-107 0.0027152024183402872 5.3239263104711516E-5 0.0 10.846115636744248 0.0017302760509031244 108-109 0.0027152024183402872 5.3239263104711516E-5 0.0 11.468216426122803 0.00175689568245548 110-111 0.0027152024183402872 5.3239263104711516E-5 0.0 12.139856349820292 0.00175689568245548 112-113 0.0027152024183402872 5.3239263104711516E-5 0.0 12.828586076974393 0.0018101349455601915 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1385 0.0 41.684685 1 GTATCAA 2080 0.0 40.919212 1 ATCAACG 2195 0.0 36.057095 3 CAACGCA 2230 0.0 35.491177 5 GTACATG 6055 0.0 35.386932 1 TCAACGC 2290 0.0 34.30142 4 TACATGG 6160 0.0 34.295162 2 ACATGGG 6045 0.0 34.159126 3 AACGCAG 2375 0.0 33.32346 6 TATCAAC 2655 0.0 30.034847 2 GTATAGG 680 0.0 27.133595 1 ACGCAGA 2940 0.0 26.914446 7 CATGGGG 3505 0.0 25.976284 4 CGCAGAG 3075 0.0 25.926315 8 CATGGGA 3225 0.0 25.463785 4 GAGTACA 4415 0.0 24.670328 1 TAAGGTG 1135 0.0 23.069088 5 TATAGGG 645 0.0 22.143013 2 TAAGGTA 715 0.0 20.806908 4 ATGGGAT 1710 0.0 20.531918 5 >>END_MODULE