FastQCFastQC Report
Thu 26 May 2016
SRR1364354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878313
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23840.12692240324163223No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21610.1150500475692816No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.066.900261
GTATCAA43100.050.8337251
ATCAACG51150.041.777043
TCAACGC52000.041.2086144
CAACGCA52100.040.9010245
AACGCAG53900.039.3006556
TATCAAC55150.038.9628372
ACGCAGA64450.032.8647967
CGCAGAG65750.032.2149968
GTACATG58350.030.8139571
TACATGG59200.030.0634172
ACATGGG58500.029.3039043
GCAGAGT78650.026.8555159
GGCGCGT450.00953306326.44364522-23
GTGGTAT14450.026.368991
CATGGGG36300.024.7604894
TGGTATC13950.024.7481442
CAGAGTA72850.024.37915410-11
AGAGTAC71400.024.29093410-11
GTGTAAG12250.023.8144951
Produced by FastQC (version 0.11.5)