FastQCFastQC Report
Thu 26 May 2016
SRR1364353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648743
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16100.048.8136631
GTATCAA26800.048.4300651
ATCAACG29250.043.3452643
CAACGCA30550.041.890465
TCAACGC30850.041.4830974
AACGCAG31350.041.006386
TATCAAC33650.038.2082022
GTACATG72800.037.7836531
TACATGG75000.035.475962
ACATGGG72600.035.3369063
ACGCAGA38350.033.509317
CGCAGAG39550.032.3411838
CATGGGG34300.029.3278684
CATGGGA46400.029.2485794
TAAGGTG7500.026.1903065
ATGGGAG24000.025.7934825
GCAGAGT50300.025.0744729
GGACCGA3103.3651304E-1023.0385276
AGAGTAC43400.023.02944610-11
GTAAGGT6750.022.0457134
Produced by FastQC (version 0.11.5)