##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364353_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1648743 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.098489576604724 31.0 31.0 34.0 28.0 34.0 2 31.170995722195638 33.0 31.0 34.0 28.0 34.0 3 31.30366042494191 33.0 31.0 34.0 28.0 34.0 4 34.72683310861669 37.0 35.0 37.0 32.0 37.0 5 34.815842735950966 37.0 35.0 37.0 32.0 37.0 6 34.861197287873246 37.0 35.0 37.0 32.0 37.0 7 34.79747722962281 37.0 35.0 37.0 32.0 37.0 8 34.70710777847123 37.0 35.0 37.0 32.0 37.0 9 36.28454707616651 39.0 37.0 39.0 32.0 39.0 10-11 36.19895399101012 39.0 37.0 39.0 32.0 39.0 12-13 36.20468229432968 39.0 37.0 39.0 32.0 39.0 14-15 37.52796706339314 40.0 38.0 41.0 32.0 41.0 16-17 37.53421636968284 40.0 38.0 41.0 32.0 41.0 18-19 37.54218031554948 40.0 38.0 41.0 32.0 41.0 20-21 37.57095769322447 40.0 38.0 41.0 32.0 41.0 22-23 37.53221969706619 40.0 38.0 41.0 31.5 41.0 24-25 37.54610785307352 40.0 38.0 41.0 31.0 41.0 26-27 37.50930951640128 40.0 38.0 41.0 31.0 41.0 28-29 37.51007676757384 40.0 38.0 41.0 31.0 41.0 30-31 37.4189179271724 40.0 38.0 41.0 31.0 41.0 32-33 37.37587816900512 40.0 38.0 41.0 30.5 41.0 34-35 37.36288706002088 40.0 38.0 41.0 31.0 41.0 36-37 37.30316853505974 40.0 38.0 41.0 30.0 41.0 38-39 37.21701835883458 40.0 37.0 41.0 30.0 41.0 40-41 37.15695017355646 40.0 37.0 41.0 30.0 41.0 42-43 37.0365087220992 40.0 37.0 41.0 30.0 41.0 44-45 36.92683577731641 40.0 37.0 41.0 30.0 41.0 46-47 36.81863425652148 40.0 37.0 41.0 30.0 41.0 48-49 36.6808550513937 40.0 36.0 41.0 30.0 41.0 50-51 36.56754933910258 40.0 36.0 41.0 30.0 41.0 52-53 36.41627591443906 40.0 36.0 41.0 30.0 41.0 54-55 36.23684376521993 39.5 35.0 41.0 29.0 41.0 56-57 36.04557744900206 39.0 35.0 41.0 29.0 41.0 58-59 35.826707982990676 39.0 35.0 41.0 29.0 41.0 60-61 35.57996061241806 38.5 35.0 41.0 28.0 41.0 62-63 35.31549459194065 38.0 35.0 40.0 28.0 41.0 64-65 34.97813607093404 37.5 34.0 40.0 28.0 41.0 66-67 34.663093641640934 37.0 34.0 40.0 28.0 41.0 68-69 34.338422058501536 36.5 34.0 39.0 27.5 41.0 70-71 33.97921962367695 36.0 34.0 39.0 26.5 41.0 72-73 33.610141180280976 35.5 33.5 39.0 26.0 40.0 74-75 33.255454609966506 35.0 33.0 37.5 26.0 40.0 76-77 33.02194217048988 35.0 33.0 37.0 26.0 39.0 78-79 32.85174099298678 35.0 33.0 37.0 26.0 39.0 80-81 32.588677252913286 35.0 33.0 36.0 26.0 39.0 82-83 32.30124828429901 35.0 33.0 36.0 26.0 37.0 84-85 32.059834067529025 35.0 33.0 35.5 26.0 37.0 86-87 31.857759820663375 35.0 33.0 35.0 25.5 36.5 88-89 31.602189668128993 35.0 33.0 35.0 25.0 36.0 90-91 31.494056987656656 35.0 33.0 35.0 25.0 36.0 92-93 31.356560422091256 35.0 33.0 35.0 24.0 36.0 94-95 31.26484085148504 35.0 33.0 35.0 24.0 35.5 96-97 31.166879859383783 35.0 32.0 35.0 24.0 35.0 98-99 31.04816821057011 35.0 32.0 35.0 23.0 35.0 100-101 30.96988584636902 35.0 32.0 35.0 23.0 35.0 102-103 29.97468162108952 34.0 30.5 35.0 19.5 35.0 104-105 30.680763466471124 34.5 32.0 35.0 20.0 35.0 106-107 31.013092095008137 35.0 32.5 35.0 23.0 35.0 108-109 31.057456498678086 35.0 33.0 35.0 23.0 35.0 110-111 30.999290065219384 35.0 33.0 35.0 23.0 35.0 112-113 30.920347804357622 35.0 33.0 35.0 20.0 35.0 114-115 30.83020185680849 35.0 33.0 35.0 19.0 35.0 116-117 30.714414557029205 35.0 33.0 35.0 18.5 35.0 118-119 30.561099880333078 35.0 32.5 35.0 15.0 35.0 120-121 30.394904481777935 35.0 32.0 35.0 4.5 35.0 122-123 30.23083797778065 35.0 32.0 35.0 2.0 35.0 124-125 29.443785356480664 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37224337413201525 1101 2 0.20594251353174897 1101 3 0.197234226921843 1101 4 0.3665711411479933 1101 5 0.2170423537387265 1101 6 0.2695172335402276 1101 7 0.27099675035169923 1101 8 0.16301196076246072 1101 9 0.3359935428414431 1101 10-11 0.4662104995288914 1101 12-13 0.3751776041563488 1101 14-15 0.5133175471057498 1101 16-17 0.5431828770652842 1101 18-19 0.3394753136847086 1101 20-21 0.49621264241145724 1101 22-23 0.599081880729706 1101 24-25 0.6163901025160854 1101 26-27 0.6700085282450416 1101 28-29 0.6127215492215825 1101 30-31 0.6285656314135863 1101 32-33 0.554184435977696 1101 34-35 0.621559421807909 1101 36-37 0.5521017886491535 1101 38-39 0.5883213483331673 1101 40-41 0.5119254016876482 1101 42-43 0.506746558486256 1101 44-45 0.44681737019186585 1101 46-47 0.5024352881645271 1101 48-49 0.4296327375798299 1101 50-51 0.41990474855457904 1101 52-53 0.3627138733034059 1101 54-55 0.5543297579028419 1101 56-57 0.4796477443483127 1101 58-59 0.48800340596293523 1101 60-61 0.40356960053247093 1101 62-63 0.32210610947363705 1101 64-65 0.4137415102672577 1101 66-67 0.37774991416407744 1101 68-69 0.31008039134790266 1101 70-71 0.36402653711544986 1101 72-73 0.34469677645962804 1101 74-75 0.3430773093582786 1101 76-77 0.3012906663974704 1101 78-79 0.3537074271048084 1101 80-81 0.4404238218113079 1101 82-83 0.2522235800802193 1101 84-85 0.2761513675414875 1101 86-87 0.286474851680687 1101 88-89 0.4533894069370099 1101 90-91 0.2819179674696848 1101 92-93 0.2667477243136318 1101 94-95 0.290300237285976 1101 96-97 0.16835735313443223 1101 98-99 0.22212175513362098 1101 100-101 0.17550833426991375 1101 102-103 -0.020646234898404003 1101 104-105 0.2090887510295012 1101 106-107 0.2675021620197384 1101 108-109 0.2802683779082322 1101 110-111 0.2755746418773768 1101 112-113 0.2650145184010988 1101 114-115 0.08809825879097133 1101 116-117 0.013683744650364815 1101 118-119 -0.047582858765689906 1101 120-121 0.00146905972793121 1101 122-123 -0.14879166632956853 1101 124-125 -0.28984294199851846 1102 1 0.3240551800629312 1102 2 0.28156021696379696 1102 3 0.3404442138944823 1102 4 0.3691588635450671 1102 5 0.29393477583644767 1102 6 0.34274333852552985 1102 7 0.2931712863976088 1102 8 0.28864040141521485 1102 9 0.45292274644594954 1102 10-11 0.4204851663357019 1102 12-13 0.2915713737143619 1102 14-15 0.4410369092208697 1102 16-17 0.5813146285061066 1102 18-19 0.5221980118212315 1102 20-21 0.5651968491257477 1102 22-23 0.5034959921872755 1102 24-25 0.579300226932105 1102 26-27 0.5672572416058017 1102 28-29 0.5679174069166848 1102 30-31 0.5824528359529495 1102 32-33 0.5693234821157205 1102 34-35 0.5120264578442502 1102 36-37 0.45511310049388953 1102 38-39 0.41079097522431596 1102 40-41 0.40192988452079703 1102 42-43 0.2477543536736846 1102 44-45 0.29498114883040216 1102 46-47 0.35505536144214034 1102 48-49 0.34441322667503016 1102 50-51 0.37490979000164515 1102 52-53 0.3683874590095755 1102 54-55 0.44504373261311514 1102 56-57 0.5145848680887752 1102 58-59 0.4205013533613382 1102 60-61 0.36716006796875433 1102 62-63 0.4317705478334659 1102 64-65 0.3127334992912765 1102 66-67 0.32592925046113663 1102 68-69 0.3951358855506655 1102 70-71 0.3688881313821568 1102 72-73 0.455769583169122 1102 74-75 0.42621028418097495 1102 76-77 0.4246688153006204 1102 78-79 0.44987342140450437 1102 80-81 0.5331811400886295 1102 82-83 0.5122198107414775 1102 84-85 0.4454758588808829 1102 86-87 0.4529840855164764 1102 88-89 0.5437448594090633 1102 90-91 0.5126612755012303 1102 92-93 0.5407186130457475 1102 94-95 0.4387225619342878 1102 96-97 0.4706897315454128 1102 98-99 0.4519383035400395 1102 100-101 0.47581760799177886 1102 102-103 0.2954908680985149 1102 104-105 0.4153272676850719 1102 106-107 0.40331947682356173 1102 108-109 0.520976940502397 1102 110-111 0.45825801081002027 1102 112-113 0.43590452088266574 1102 114-115 0.45415099542487525 1102 116-117 0.41302978352084097 1102 118-119 0.39074251069389376 1102 120-121 0.26033927439925364 1102 122-123 0.30656559772218017 1102 124-125 0.2362400642005298 1103 1 0.2565917208253339 1103 2 0.22538467140742213 1103 3 0.22631139438935932 1103 4 0.2759972938253199 1103 5 0.3333811522846588 1103 6 0.3140128830299034 1103 7 0.262691807275516 1103 8 0.3923369938511456 1103 9 0.4245755499257342 1103 10-11 0.513189210007468 1103 12-13 0.48416394864234036 1103 14-15 0.5876878049226448 1103 16-17 0.3914726606603409 1103 18-19 0.4051226150715479 1103 20-21 0.528921812946102 1103 22-23 0.5374793100312729 1103 24-25 0.5233018321910308 1103 26-27 0.4746853796962185 1103 28-29 0.46279764615204755 1103 30-31 0.5375466373096458 1103 32-33 0.5468701272880949 1103 34-35 0.4731312929713454 1103 36-37 0.4304503002573199 1103 38-39 0.36219356680447845 1103 40-41 0.40013946892498353 1103 42-43 0.27120929195706367 1103 44-45 0.27820496662124583 1103 46-47 0.2224225460251077 1103 48-49 0.22911274482480337 1103 50-51 0.14407478282021913 1103 52-53 0.2340631939810507 1103 54-55 0.1765924531754237 1103 56-57 0.0440242675202569 1103 58-59 0.09849636043374943 1103 60-61 0.06127648058298263 1103 62-63 0.0031561942567890355 1103 64-65 0.059292174366696315 1103 66-67 0.07633550497507713 1103 68-69 0.036758593832338704 1103 70-71 0.09676914871840836 1103 72-73 0.10904745239911051 1103 74-75 0.09559252522055317 1103 76-77 0.11269141020576257 1103 78-79 0.11925276670960017 1103 80-81 0.2076568077428007 1103 82-83 0.22551944226777465 1103 84-85 0.14299680355804867 1103 86-87 0.1179569746282354 1103 88-89 0.14953915597043022 1103 90-91 0.03232382363855635 1103 92-93 0.06898098157940069 1103 94-95 0.06716303517224986 1103 96-97 0.14960762623061186 1103 98-99 0.0961370231484544 1103 100-101 0.02669293742415846 1103 102-103 -0.20011562134465777 1103 104-105 -0.032463150636139915 1103 106-107 0.05870101198527422 1103 108-109 0.1417241285738804 1103 110-111 0.06442653846871238 1103 112-113 0.10772565170445603 1103 114-115 0.053074906424303236 1103 116-117 0.034798484514794836 1103 118-119 0.013645658255075688 1103 120-121 -0.19650320990927383 1103 122-123 -0.3215301127752319 1103 124-125 -0.30700738959717455 1104 1 0.36068602768126823 1104 2 0.3571424485140362 1104 3 0.4015322491160269 1104 4 0.4827229809289122 1104 5 0.4504058427105093 1104 6 0.4539819256152242 1104 7 0.47169210929907024 1104 8 0.47467616630660103 1104 9 0.47089841310906166 1104 10-11 0.5799411354076511 1104 12-13 0.49747186165955526 1104 14-15 0.5743091051340627 1104 16-17 0.6859361913157329 1104 18-19 0.678378429025031 1104 20-21 0.6910235193792076 1104 22-23 0.6739420478748173 1104 24-25 0.7043130448156134 1104 26-27 0.7177813099287818 1104 28-29 0.7130383211747002 1104 30-31 0.6889123500852463 1104 32-33 0.6182515075357529 1104 34-35 0.5988250033307594 1104 36-37 0.641437350997279 1104 38-39 0.53485194040762 1104 40-41 0.6089793209334431 1104 42-43 0.5817036667682984 1104 44-45 0.5699314087837379 1104 46-47 0.611046639954445 1104 48-49 0.46330841833734837 1104 50-51 0.518296128335173 1104 52-53 0.4868097207101343 1104 54-55 0.44154261928838423 1104 56-57 0.42146877069151145 1104 58-59 0.3511437856829147 1104 60-61 0.3288315998822853 1104 62-63 0.36167703388228034 1104 64-65 0.41121096778258703 1104 66-67 0.3375575677959759 1104 68-69 0.3836137735363536 1104 70-71 0.3014750532789634 1104 72-73 0.2674116173039778 1104 74-75 0.2801241999411772 1104 76-77 0.3194642884902734 1104 78-79 0.3951182529675279 1104 80-81 0.3603108397572967 1104 82-83 0.31651819642059564 1104 84-85 0.2679543314952326 1104 86-87 0.325215922076147 1104 88-89 0.33859646494052953 1104 90-91 0.2727392947953078 1104 92-93 0.25284726064916896 1104 94-95 0.22065707020698255 1104 96-97 0.18695472680445846 1104 98-99 0.1343382840968097 1104 100-101 0.15496800537945887 1104 102-103 -0.09221298618911788 1104 104-105 0.13888330813764327 1104 106-107 0.22501689087382815 1104 108-109 0.23820177883059657 1104 110-111 0.14651559766532785 1104 112-113 0.1545817752320886 1104 114-115 0.0903465011086908 1104 116-117 0.03271735343656701 1104 118-119 0.0790715114716285 1104 120-121 0.058404672905588484 1104 122-123 -0.07802833838682943 1104 124-125 -0.33070208153314695 1105 1 0.12183720646950391 1105 2 0.11127650489458674 1105 3 0.09315124944792785 1105 4 0.1776777324840353 1105 5 0.06705709644440816 1105 6 0.18970410739058252 1105 7 0.17175420713528666 1105 8 0.18735456375416248 1105 9 0.22419066908062746 1105 10-11 0.2452553785635061 1105 12-13 0.2570722674367971 1105 14-15 0.3507871741590165 1105 16-17 0.36962634184945387 1105 18-19 0.23354713706883956 1105 20-21 0.28513084960051316 1105 22-23 0.3651747769141309 1105 24-25 0.39527811076779784 1105 26-27 0.40420480245027335 1105 28-29 0.48170497758094655 1105 30-31 0.49405479368863325 1105 32-33 0.5301532136202596 1105 34-35 0.4527256240414559 1105 36-37 0.42590650148817844 1105 38-39 0.3433923669019805 1105 40-41 0.3718703097879512 1105 42-43 0.40315080732354147 1105 44-45 0.2645776786309568 1105 46-47 0.34584187100121966 1105 48-49 0.2168132991737366 1105 50-51 0.26355765524601793 1105 52-53 0.31996160077566316 1105 54-55 0.35181526090892135 1105 56-57 0.31378356121837214 1105 58-59 0.29009937100855154 1105 60-61 0.17856688108913232 1105 62-63 0.20714509632271927 1105 64-65 0.20745127168725475 1105 66-67 0.1731639183703848 1105 68-69 0.08759713559886251 1105 70-71 0.00796273810033199 1105 72-73 0.04127545441492941 1105 74-75 0.0672857065096295 1105 76-77 0.13039979774605825 1105 78-79 0.28307465198895443 1105 80-81 0.26815845380853887 1105 82-83 0.18709623493196403 1105 84-85 0.2316061861327583 1105 86-87 0.28019013942927273 1105 88-89 0.2648087779778656 1105 90-91 0.1621002558663882 1105 92-93 0.18343185021458908 1105 94-95 0.17817592707147156 1105 96-97 0.13294600366445053 1105 98-99 0.25593344233922366 1105 100-101 0.1165031468661688 1105 102-103 0.06294009702198267 1105 104-105 0.023195474227943436 1105 106-107 0.06241407479048888 1105 108-109 0.05189354872599594 1105 110-111 0.17654388296206136 1105 112-113 0.15713210258404686 1105 114-115 0.046023835933795 1105 116-117 0.06838329250574304 1105 118-119 0.02882178181134165 1105 120-121 0.029329100909571082 1105 122-123 0.027801464024282296 1105 124-125 -0.091565355213298 1106 1 0.2577802876714763 1106 2 0.3711774311737166 1106 3 0.32414619791782684 1106 4 0.44901253039308386 1106 5 0.2905497855759833 1106 6 0.26105721800527704 1106 7 0.2784438980075592 1106 8 0.30856451132164864 1106 9 0.44472390598026834 1106 10-11 0.472677696661151 1106 12-13 0.3569586559398559 1106 14-15 0.4096605394855999 1106 16-17 0.43533507330783294 1106 18-19 0.4607489754793761 1106 20-21 0.3323930009401508 1106 22-23 0.4319832623740325 1106 24-25 0.4194669141018963 1106 26-27 0.5651739809105649 1106 28-29 0.4729720647567035 1106 30-31 0.637364772262103 1106 32-33 0.5918280262455866 1106 34-35 0.5216620525085176 1106 36-37 0.5499370685011087 1106 38-39 0.5002892822357978 1106 40-41 0.6080169462992515 1106 42-43 0.5149442558704536 1106 44-45 0.5685875992252107 1106 46-47 0.5024660790758659 1106 48-49 0.4825853648271021 1106 50-51 0.4850359467565184 1106 52-53 0.47674563183189633 1106 54-55 0.502904172730922 1106 56-57 0.47074840545128893 1106 58-59 0.4561833807109821 1106 60-61 0.5089508991876315 1106 62-63 0.5335720494521112 1106 64-65 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-1.4341871846902876 2216 78-79 -1.5030289166910684 2216 80-81 -1.4209605204614277 2216 82-83 -1.4300872151346056 2216 84-85 -1.473830415959533 2216 86-87 -1.5147516328758321 2216 88-89 -1.421556384287804 2216 90-91 -1.6025434470473385 2216 92-93 -1.550637602825308 2216 94-95 -1.3629636892274917 2216 96-97 -1.4107210565534558 2216 98-99 -1.5769598101219948 2216 100-101 -1.5339181603938243 2216 102-103 -1.5769792154584614 2216 104-105 -1.5589837053889966 2216 106-107 -1.409312465711416 2216 108-109 -1.4256603285910643 2216 110-111 -1.4403940733423326 2216 112-113 -1.3887766582319507 2216 114-115 -1.3663585839724348 2216 116-117 -1.3967989837580888 2216 118-119 -1.3711209553413397 2216 120-121 -1.2124271614650297 2216 122-123 -1.3804783554750024 2216 124-125 -1.231177515033167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25680.0 3 4919.0 4 3065.0 5 2572.0 6 2731.0 7 3184.0 8 3586.0 9 3921.0 10 4243.0 11 4979.0 12 5722.0 13 5725.0 14 5506.0 15 5050.0 16 4797.0 17 4305.0 18 4024.0 19 3887.0 20 3896.0 21 4119.0 22 5051.0 23 6586.0 24 9707.0 25 14147.0 26 16951.0 27 17068.0 28 20193.0 29 24767.0 30 29166.0 31 36539.0 32 46583.0 33 62383.0 34 87590.0 35 134456.0 36 266487.0 37 527511.0 38 236974.0 39 673.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97226941747573 18.897148058252426 12.803519417475728 24.327063106796114 2 15.474799677299997 20.23456408203374 39.570420783821326 24.72021545684494 3 18.42849873020475 25.16881574533979 29.451728674445366 26.9509568500101 4 12.1964386338533 16.373586180584326 37.52815204317264 33.901823142389745 5 13.098020848942538 38.77578135000634 34.78587154416007 13.340326256891052 6 32.241378368917175 36.902395060994365 17.560565126887816 13.295661443200647 7 28.5089804959592 32.49221803571818 21.55367553758028 17.445125930742336 8 26.303298276259447 35.63078472045319 20.052524958453123 18.013392044834237 9 26.405146223516944 16.072608041398812 19.593957335982623 37.92828839910162 10-11 24.610991525058786 26.99295766532443 28.07035420317175 20.32569660644503 12-13 25.11014538462938 24.393169287876123 27.978716951835654 22.51796837565884 14-15 22.33663636570406 25.91670899703561 25.070607055398447 26.676047581861877 16-17 22.575181562670206 29.133727411565907 25.90132355456463 22.389767471199253 18-19 22.61477090701329 28.144020194803186 27.070413121284666 22.170795776898856 20-21 23.2410387792397 27.47614394723738 26.96014478909084 22.32267248443208 22-23 23.12037810634468 27.304566965429718 26.743472676903764 22.83158225132184 24-25 22.852045467365137 27.42446821608947 26.952836190964874 22.77065012558052 26-27 22.65445412320424 27.733659966204538 26.69222959080317 22.919656319788057 28-29 22.73598875746518 27.71464365028172 26.79836735783146 22.75100023442165 30-31 22.768962965658623 27.362046168589348 26.78251270667297 23.086478159079054 32-33 22.51570595206758 27.516985718773995 26.735175631301033 23.23213269785739 34-35 23.14647317614756 27.473080012870525 26.45211143011114 22.928335380870774 36-37 23.16893728673134 27.12143561576038 26.52992402692477 23.179703070583514 38-39 22.77593798900979 27.370385870422258 26.63634047818334 23.21733566238461 40-41 22.987994731716963 27.166896792661284 26.764375950160563 23.080732525461194 42-43 22.7545912583678 27.188910744081358 26.742385507399323 23.314112490151516 44-45 22.742298130128592 27.284480170410674 26.850689897575275 23.122531801885458 46-47 23.04061797475103 27.55688357264227 26.43041764304328 22.972080809563423 48-49 22.85768612815945 27.3567499604244 26.513107258074786 23.272456653341365 50-51 22.797670710353284 27.42461984675598 26.66655749258678 23.11115195030396 52-53 23.06584461979827 27.246880932596607 26.547251520867576 23.14002292673755 54-55 22.781850361558906 27.28553630934546 26.72967209197098 23.20294123712466 56-57 22.685916102043922 27.57419145881615 26.722844885885657 23.017047553254272 58-59 22.76034364642593 27.131497313537484 26.650490574712226 23.45766846532436 60-61 22.50648228176318 27.316320186811023 26.97335820103413 23.203839330391666 62-63 22.60265622469645 27.32310486064737 27.03251773636815 23.04172117828803 64-65 22.495788393099485 27.28490478205029 26.807260795133185 23.412046029717036 66-67 22.439685959395987 27.53938851364466 26.651054403943945 23.36987112301541 68-69 22.559644178968323 27.21302436959193 26.962007504121445 23.2653239473183 70-71 22.576652808411875 27.12494840683647 26.881001069619913 23.41739771513174 72-73 22.657912294535087 27.25598956753806 26.656335294474438 23.429762843452416 74-75 22.689500714953986 27.482467326678627 26.586159158007106 23.241872800360284 76-77 22.849614079758165 27.20947716154608 26.737414414745004 23.203494343950755 78-79 22.818456111343448 27.30853784541219 26.771357199200217 23.101648844044142 80-81 22.87866689431781 27.468496043752687 26.625721809292834 23.027115252636666 82-83 22.740541845389437 27.445994992527613 26.641772849018885 23.17169031306407 84-85 22.849839406840957 27.345750354135888 26.691732401546282 23.112677837476873 86-87 22.850179852351545 27.192868864117386 26.84414241770932 23.112808865821748 88-89 22.874727770268283 27.250996040101818 26.734604936695312 23.13967125293459 90-91 22.842687623333155 27.42547243023517 26.740250331238695 22.991589615192975 92-93 22.85487010249751 27.003383831001326 26.847232676441102 23.294513390060064 94-95 22.706572189764533 26.96485623003195 26.91785028150498 23.41072129869854 96-97 22.90571510192347 26.848187430742442 26.94874925473256 23.297348212601523 98-99 22.785617804702156 26.962097562188664 26.74432427809718 23.507960355012 100-101 22.615686061629077 26.979347899595957 26.9664894280012 23.438476610773762 102-103 22.61978701027101 26.72973789332075 27.023329641576062 23.62714545483218 104-105 22.62545190099407 27.00540021877452 26.826595872761423 23.542552007469993 106-107 22.636877255458213 27.026890960698204 26.72988849290582 23.60634329093777 108-109 22.793667429988638 27.004857420735974 26.540071067489247 23.661404081786145 110-111 22.708557055477428 27.204208367059085 26.721620558549976 23.36561401891351 112-113 22.672401688481322 27.32794707104171 26.908525835953835 23.09112540452314 114-115 22.572980271110737 27.464139906677932 26.510084618235346 23.452795203975985 116-117 22.558429471131667 27.335806567343063 26.80057268721131 23.30519127431396 118-119 22.566023035055625 27.529200381197068 26.421744616774674 23.48303196697263 120-121 22.49615946303758 27.72126118229395 26.58679765185096 23.195781702817506 122-123 22.6416622161001 27.734111174091304 26.517188285198976 23.107038324609615 124-125 22.601298781251526 27.81559040928926 26.431408513194793 23.15170229626442 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 619.0 1 516.5 2 368.0 3 285.5 4 225.5 5 210.5 6 195.5 7 164.0 8 162.5 9 170.0 10 151.5 11 132.0 12 138.5 13 163.0 14 210.0 15 254.0 16 278.0 17 309.0 18 428.5 19 560.5 20 733.5 21 1073.0 22 1519.5 23 2166.0 24 2979.5 25 4165.0 26 5969.5 27 8366.5 28 10959.0 29 13899.5 30 17683.0 31 21491.5 32 25623.5 33 28950.0 34 31492.5 35 34373.5 36 38740.5 37 43945.0 38 47081.5 39 49348.0 40 51172.0 41 53712.0 42 56154.5 43 58071.0 44 60212.0 45 62529.5 46 62744.5 47 61701.0 48 62120.0 49 61634.5 50 60241.0 51 58814.5 52 58602.5 53 58934.5 54 59421.5 55 56631.0 56 50163.0 57 45037.5 58 42153.0 59 37654.0 60 30851.0 61 25365.5 62 20435.0 63 15145.5 64 11070.0 65 8302.5 66 6151.5 67 5188.0 68 4021.0 69 2538.5 70 1784.0 71 1272.5 72 923.5 73 683.5 74 487.0 75 362.5 76 254.5 77 140.5 78 84.0 79 50.0 80 27.5 81 22.5 82 17.0 83 12.5 84 7.5 85 5.5 86 7.0 87 4.5 88 6.0 89 5.0 90 2.5 91 2.0 92 2.5 93 1.5 94 1.5 95 2.0 96 1.5 97 2.0 98 2.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04506463408790818 2 0.009279796790645965 3 0.003153917863487517 4 0.006429140260186093 5 0.024867429308266964 6 0.028870478904231892 7 0.003578483729726222 8 1.8195679981658754E-4 9 0.0 10-11 0.0 12-13 7.884794658718793E-4 14-15 3.335874663304105E-4 16-17 6.0652266605529186E-5 18-19 1.2130453321105837E-4 20-21 0.0 22-23 3.0326133302764593E-5 24-25 0.0 26-27 6.0652266605529186E-5 28-29 2.7293519972488136E-4 30-31 1.8195679981658754E-4 32-33 3.032613330276459E-4 34-35 8.794578657801732E-4 36-37 6.0652266605529186E-5 38-39 1.8195679981658754E-4 40-41 5.15544266146998E-4 42-43 7.278271992663502E-4 44-45 0.0012130453321105837 46-47 1.5163066651382296E-4 48-49 0.0 50-51 0.0 52-53 7.884794658718793E-4 54-55 0.00297196106367093 56-57 1.2130453321105837E-4 58-59 8.18805599174644E-4 60-61 0.0010917407988995254 62-63 7.278271992663502E-4 64-65 0.001546632798440994 66-67 0.002365438397615638 68-69 0.00297196106367093 70-71 0.0016072850650465233 72-73 0.002608047464037755 74-75 0.002153155464496286 76-77 0.0013040237320188774 78-79 0.0025777213307349904 80-81 0.0013040237320188774 82-83 4.2456586623870426E-4 84-85 6.975010659635856E-4 86-87 0.001789241864863111 88-89 0.005428377861194862 90-91 4.548919995414688E-4 92-93 0.0012130453321105837 94-95 9.401101323857023E-4 96-97 3.639135996331751E-4 98-99 6.065226660552918E-4 100-101 0.001485980531835465 102-103 0.0015163066651382295 104-105 8.18805599174644E-4 106-107 0.00627750959367227 108-109 0.0017285895982575817 110-111 0.0016679373316520526 112-113 0.0025777213307349904 114-115 0.002668699730643284 116-117 0.001485980531835465 118-119 0.04709648501919341 120-121 0.012767302120463892 122-123 0.004639898395322983 124-125 0.0026383735973405193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1648743.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.47896392976163 #Duplication Level Percentage of deduplicated Percentage of total 1 85.25307847890963 61.79054799974028 2 9.346572519331643 13.548597849910788 3 2.5870897683859524 5.625287580177025 4 1.0295600642720646 2.984857870475921 5 0.5156362187166188 1.8686389448620246 6 0.3001473169333886 1.305261993457788 7 0.18443100137040397 0.9357157527098728 8 0.1336386064388924 0.7748790188566472 9 0.09762482707194274 0.63681716879969 >10 0.49845708774401376 6.7211716050486965 >50 0.03766950814586697 1.9019860497794903 >100 0.015928678949073828 1.8123152616801574 >500 1.659237306632312E-4 0.09392290450158651 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 5 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 6 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 7 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 8 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 9 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 10-11 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 12-13 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 14-15 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 16-17 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 18-19 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 20-21 0.0 0.0 0.0 6.065226660552918E-5 6.065226660552918E-5 22-23 0.0 0.0 0.0 6.065226660552918E-5 9.097839990829377E-5 24-25 0.0 0.0 0.0 2.1228293311935213E-4 1.2130453321105836E-4 26-27 0.0 0.0 0.0 5.458703994497626E-4 1.2130453321105836E-4 28-29 0.0 0.0 0.0 0.002729351997248813 1.2130453321105836E-4 30-31 0.0 0.0 0.0 0.010462515989453783 1.2130453321105836E-4 32-33 0.0 0.0 0.0 0.02653536663991902 1.2130453321105836E-4 34-35 0.0 0.0 0.0 0.05373790821249885 1.2130453321105836E-4 36-37 0.0 0.0 0.0 0.09740754016847987 1.2130453321105836E-4 38-39 0.0 0.0 0.0 0.1636701414350205 1.2130453321105836E-4 40-41 0.0 0.0 0.0 0.23636188296174723 1.2130453321105836E-4 42-43 0.0 0.0 0.0 0.30714307809039976 1.2130453321105836E-4 44-45 0.0 0.0 0.0 0.3955437566679586 1.2130453321105836E-4 46-47 0.0 0.0 0.0 0.5055972944236913 1.2130453321105836E-4 48-49 0.0 0.0 0.0 0.6263256311019971 1.2130453321105836E-4 50-51 0.0 0.0 0.0 0.7581836587024176 1.2130453321105836E-4 52-53 0.0 0.0 0.0 0.8810651508452196 1.2130453321105836E-4 54-55 0.0 0.0 0.0 1.0145304635106867 1.5163066651382296E-4 56-57 0.0 0.0 0.0 1.1707100500199243 1.8195679981658754E-4 58-59 0.0 0.0 0.0 1.3465712970426562 1.8195679981658754E-4 60-61 0.0 0.0 0.0 1.5368981096508068 1.8195679981658754E-4 62-63 0.0 0.0 0.0 1.727770792658407 1.8195679981658754E-4 64-65 0.0 0.0 0.0 1.918704127932613 1.8195679981658754E-4 66-67 0.0 0.0 0.0 2.1431478405063737 1.8195679981658754E-4 68-69 0.0 0.0 0.0 2.397917686382899 1.8195679981658754E-4 70-71 0.0 0.0 0.0 2.6753714799698924 1.8195679981658754E-4 72-73 0.0 0.0 0.0 2.9631361588798253 1.8195679981658754E-4 74-75 0.0 0.0 0.0 3.240377669533699 1.8195679981658754E-4 76-77 0.0 0.0 0.0 3.542274326562721 1.8195679981658754E-4 78-79 0.0 0.0 0.0 3.8858694168830437 1.8195679981658754E-4 80-81 0.0 0.0 0.0 4.258698899707232 1.8195679981658754E-4 82-83 0.0 0.0 0.0 4.6488749307806 2.4260906642211672E-4 84-85 0.0 0.0 0.0 5.036048674656996 2.4260906642211672E-4 86-87 0.0 0.0 0.0 5.431895692657982 2.4260906642211672E-4 88-89 0.0 0.0 0.0 5.867348640752379 2.4260906642211672E-4 90-91 0.0 0.0 0.0 6.349473507999731 2.4260906642211672E-4 92-93 0.0 0.0 0.0 6.84297067523562 2.4260906642211672E-4 94-95 0.0 0.0 0.0 7.343746114464171 2.4260906642211672E-4 96-97 0.0 0.0 0.0 7.833513167303819 2.4260906642211672E-4 98-99 0.0 0.0 0.0 8.340171876393107 2.4260906642211672E-4 100-101 0.0 0.0 0.0 8.90896883261976 2.4260906642211672E-4 102-103 0.0 0.0 0.0 9.525074556798725 2.4260906642211672E-4 104-105 0.0 0.0 0.0 10.14303017510916 2.4260906642211672E-4 106-107 0.0 0.0 0.0 10.733601295047196 2.4260906642211672E-4 108-109 0.0 0.0 0.0 11.343611466432307 2.729351997248813E-4 110-111 0.0 0.0 0.0 12.008693895895238 3.032613330276459E-4 112-113 0.0 0.0 0.0 12.705982678925704 3.032613330276459E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1610 0.0 48.813663 1 GTATCAA 2680 0.0 48.430065 1 ATCAACG 2925 0.0 43.345264 3 CAACGCA 3055 0.0 41.89046 5 TCAACGC 3085 0.0 41.483097 4 AACGCAG 3135 0.0 41.00638 6 TATCAAC 3365 0.0 38.208202 2 GTACATG 7280 0.0 37.783653 1 TACATGG 7500 0.0 35.47596 2 ACATGGG 7260 0.0 35.336906 3 ACGCAGA 3835 0.0 33.50931 7 CGCAGAG 3955 0.0 32.341183 8 CATGGGG 3430 0.0 29.327868 4 CATGGGA 4640 0.0 29.248579 4 TAAGGTG 750 0.0 26.190306 5 ATGGGAG 2400 0.0 25.793482 5 GCAGAGT 5030 0.0 25.074472 9 GGACCGA 310 3.3651304E-10 23.038527 6 AGAGTAC 4340 0.0 23.029446 10-11 GTAAGGT 675 0.0 22.045713 4 >>END_MODULE