FastQCFastQC Report
Thu 26 May 2016
SRR1364353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648743
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31670.1920857283397109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29620.17965201368557743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.073.354891
GTATCAA49800.051.057291
ATCAACG59750.041.9505353
TCAACGC61450.040.789984
CAACGCA62400.040.2643975
TATCAAC63200.039.754722
AACGCAG63750.039.5805056
TGGTATC12650.037.1817552
GTACATG70850.035.9718781
TACATGG72150.034.5757032
ACATGGG70800.034.1417733
ACGCAGA75550.033.3126647
CGCAGAG76500.032.82128
CATGGGA44950.030.9941334
GTGGTAT16550.030.9428041
GCAGAGT87550.027.9991289
GAGTACT51850.026.85162712-13
CAGAGTA83900.025.88417610-11
GTACTTT55100.024.94386914-15
CATGGGG36200.024.1769814
Produced by FastQC (version 0.11.5)