FastQCFastQC Report
Thu 26 May 2016
SRR1364352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496588
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.052.9938471
GTATCAA25900.047.354761
ATCAACG29150.040.631753
TCAACGC29600.040.0140344
CAACGCA30050.039.8109555
GTACATG75100.037.4195251
AACGCAG32150.037.2080656
TACATGG77400.035.9890562
TATCAAC33050.035.838282
ACATGGG77500.034.4054573
CATGGGG33150.030.8813254
ACGCAGA38650.030.6333837
CGCAGAG40250.029.413698
CATGGGA51850.027.5494864
ATGGGGA19950.023.8670165
CGTTTAA2802.590241E-923.3900341
TAAGGTA5900.023.2020764
GTGGTAT9850.022.9690781
GCAGAGT50350.022.6863239
GAGTACA56600.022.5109331
Produced by FastQC (version 0.11.5)