FastQCFastQC Report
Thu 26 May 2016
SRR1364352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496588
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38160.2549799944941427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35960.24027988998976338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.13831461965484154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.073.080561
GTATCAA49500.056.3099441
ATCAACG64650.043.007833
TCAACGC65150.042.7691544
CAACGCA66400.041.8743445
TATCAAC66700.041.775272
AACGCAG69250.040.2168056
GTACATG70700.036.5607221
ACGCAGA79800.034.9674847
CGCAGAG80850.034.513368
TACATGG74900.034.1015972
ACATGGG73700.033.8489953
GTGGTAT10650.031.8764321
GCAGAGT91200.030.2703579
CATGGGA48600.029.8918484
ACGTGCG800.005345704529.7693395
CAGAGTA86450.027.66657810-11
GAGTACT55300.027.65046312-13
CATGGGG31700.027.6094234
TAAGGTG8050.027.3656045
Produced by FastQC (version 0.11.5)