##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364352_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1496588 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.280421866271816 33.0 31.0 34.0 30.0 34.0 2 32.300212884240686 33.0 31.0 34.0 30.0 34.0 3 32.41241544098977 34.0 31.0 34.0 30.0 34.0 4 36.022170430338875 37.0 35.0 37.0 35.0 37.0 5 35.957527388967435 37.0 35.0 37.0 35.0 37.0 6 36.020141147730705 37.0 35.0 37.0 35.0 37.0 7 35.974939662752874 37.0 35.0 37.0 35.0 37.0 8 35.982388606617185 37.0 35.0 37.0 35.0 37.0 9 37.73543687374214 39.0 38.0 39.0 35.0 39.0 10-11 37.69460866985436 39.0 37.5 39.0 35.0 39.0 12-13 37.658966261923794 39.0 37.0 39.0 35.0 39.0 14-15 39.05367275429177 41.0 38.0 41.0 35.5 41.0 16-17 39.061222260234615 41.0 38.0 41.0 35.5 41.0 18-19 39.12000898042748 41.0 38.5 41.0 35.5 41.0 20-21 39.12789592058736 41.0 39.0 41.0 35.0 41.0 22-23 39.08970605136484 41.0 39.0 41.0 35.0 41.0 24-25 39.05411843473287 41.0 39.0 41.0 35.0 41.0 26-27 38.9572089980676 41.0 38.5 41.0 35.0 41.0 28-29 38.86500125619075 40.5 38.0 41.0 35.0 41.0 30-31 38.732595744453384 40.0 38.0 41.0 35.0 41.0 32-33 38.5977877679094 40.0 38.0 41.0 34.0 41.0 34-35 38.50022317431384 40.0 38.0 41.0 34.0 41.0 36-37 38.37799614857262 40.0 38.0 41.0 34.0 41.0 38-39 38.28637808134236 40.0 38.0 41.0 34.0 41.0 40-41 38.16855874829946 40.0 38.0 41.0 33.0 41.0 42-43 38.00937365527453 40.0 38.0 41.0 33.0 41.0 44-45 37.828004768179355 40.0 37.0 41.0 33.0 41.0 46-47 37.57025948357196 40.0 37.0 41.0 32.5 41.0 48-49 37.328556356191555 40.0 37.0 41.0 32.0 41.0 50-51 37.16043393372124 40.0 37.0 41.0 31.5 41.0 52-53 36.99809199325399 40.0 36.0 41.0 31.0 41.0 54-55 36.81284595359578 40.0 36.0 41.0 31.0 41.0 56-57 36.61040880990627 39.0 35.5 41.0 31.0 41.0 58-59 36.388160936744114 39.0 35.0 41.0 30.5 41.0 60-61 36.12824371169621 39.0 35.0 41.0 30.0 41.0 62-63 35.887811475168846 38.0 35.0 41.0 30.0 41.0 64-65 35.59290399228111 37.5 35.0 40.0 29.5 41.0 66-67 35.27146215257639 37.0 35.0 40.0 29.0 41.0 68-69 34.933172322643244 36.5 34.0 39.5 29.0 41.0 70-71 34.66369167733538 36.0 34.0 39.0 29.0 41.0 72-73 34.4608713286489 36.0 34.0 39.0 29.0 41.0 74-75 34.12859083461848 35.0 34.0 38.0 29.0 40.0 76-77 33.79807168038231 35.0 34.0 37.0 29.0 39.0 78-79 33.48233047438573 35.0 34.0 37.0 29.0 39.0 80-81 33.195047668429794 35.0 34.0 36.0 29.0 38.5 82-83 32.926183759324545 35.0 34.0 36.0 28.0 37.0 84-85 32.700180343554806 35.0 34.0 35.5 27.5 37.0 86-87 32.52206251820809 35.0 34.0 35.0 27.0 36.5 88-89 32.36517832563137 35.0 33.0 35.0 27.0 36.0 90-91 32.22849074027054 35.0 33.0 35.0 27.0 36.0 92-93 32.10511877684439 35.0 33.0 35.0 26.5 36.0 94-95 32.02291245152306 35.0 33.0 35.0 26.5 35.5 96-97 31.943082865825467 35.0 33.0 35.0 26.0 35.0 98-99 31.873321181246943 35.0 33.0 35.0 26.0 35.0 100-101 31.814937711648096 35.0 33.0 35.0 25.0 35.0 102-103 30.83438695218724 34.0 31.5 35.0 24.5 35.0 104-105 31.55673705789436 35.0 32.5 35.0 25.0 35.0 106-107 31.853869936148094 35.0 33.0 35.0 26.5 35.0 108-109 31.87161530093787 35.0 33.0 35.0 26.5 35.0 110-111 31.837736571454535 35.0 33.0 35.0 26.0 35.0 112-113 31.77210762080145 35.0 33.0 35.0 25.0 35.0 114-115 31.694000620077134 35.0 33.0 35.0 25.0 35.0 116-117 31.637137943107923 35.0 33.0 35.0 25.0 35.0 118-119 31.529070458937262 35.0 33.0 35.0 25.0 35.0 120-121 31.401206945398464 35.0 33.0 35.0 25.0 35.0 122-123 31.258645331914998 35.0 33.0 35.0 24.0 35.0 124-125 30.566678671752012 34.5 32.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15407636653958434 1101 2 0.16605549075707415 1101 3 0.16478854573660584 1101 4 0.11995041516353666 1101 5 0.25882692522242223 1101 6 0.2749450570807568 1101 7 0.28140248891272535 1101 8 0.30540035777318764 1101 9 0.31271980351873196 1101 10-11 0.3048432210236669 1101 12-13 0.2691250504876237 1101 14-15 0.3917726464041493 1101 16-17 0.4286874570742114 1101 18-19 0.3358426915773265 1101 20-21 0.3611718187218713 1101 22-23 0.2855548666515162 1101 24-25 0.34069734675495056 1101 26-27 0.3604801514008571 1101 28-29 0.2576154625200857 1101 30-31 0.3232242100878153 1101 32-33 0.2526764750557291 1101 34-35 0.2720537643281702 1101 36-37 0.4407118697414134 1101 38-39 0.3664169148810146 1101 40-41 0.297722066622093 1101 42-43 0.23893597774235786 1101 44-45 0.4263434601210747 1101 46-47 0.5806631032714122 1101 48-49 0.38225224962066306 1101 50-51 0.3447879599438437 1101 52-53 0.3275464931507983 1101 54-55 0.39013667508144323 1101 56-57 0.37805203708524715 1101 58-59 0.26029278430711855 1101 60-61 0.34981972521539717 1101 62-63 0.24420005137385914 1101 64-65 0.07732314077009761 1101 66-67 0.12005776118195399 1101 68-69 0.2081153243469629 1101 70-71 0.14968541901174603 1101 72-73 0.1683900680941548 1101 74-75 0.21047773553491567 1101 76-77 0.05920185566547076 1101 78-79 -0.12197012261717077 1101 80-81 -0.01681094886762935 1101 82-83 -0.10365306289754983 1101 84-85 -0.24251980458654288 1101 86-87 -0.12697865847535184 1101 88-89 -0.16573183088499377 1101 90-91 -0.4905604486892514 1101 92-93 -0.2857249320331654 1101 94-95 -0.09584025727922096 1101 96-97 -0.009553282003281538 1101 98-99 0.028400641614538813 1101 100-101 -0.24234334076812658 1101 102-103 -0.014150011096116799 1101 104-105 0.10793125333565357 1101 106-107 0.09454580494629994 1101 108-109 0.1556743745086635 1101 110-111 0.16459000216895703 1101 112-113 0.14286653087545176 1101 114-115 0.014285370183404922 1101 116-117 0.017958363233919528 1101 118-119 -0.03204127900735898 1101 120-121 0.06809827573803062 1101 122-123 0.19759906920249293 1101 124-125 0.1760254655031126 1102 1 0.02218465470001263 1102 2 0.09380683832710446 1102 3 0.039356605871901706 1102 4 0.07087628218792474 1102 5 0.027645195396033273 1102 6 0.1532934981445493 1102 7 0.10374311352478571 1102 8 0.2036436118941296 1102 9 0.23319016583045737 1102 10-11 0.1899146381693413 1102 12-13 0.28022738469734776 1102 14-15 0.3090632519424048 1102 16-17 0.3008092733287171 1102 18-19 0.30853338691584753 1102 20-21 0.3683941311835426 1102 22-23 0.3753061289915891 1102 24-25 0.375414658132712 1102 26-27 0.3587905807272094 1102 28-29 0.3765932918201287 1102 30-31 0.3935228669748625 1102 32-33 0.3656753405091351 1102 34-35 0.2774612345486318 1102 36-37 0.3745126914487784 1102 38-39 0.3321241063336444 1102 40-41 0.2634327578555755 1102 42-43 0.22871157385282714 1102 44-45 0.12399348080065664 1102 46-47 0.2660097473006644 1102 48-49 0.26935868639701965 1102 50-51 0.0016183895913499668 1102 52-53 0.13310175705795046 1102 54-55 0.1570249190499311 1102 56-57 0.22632119987796528 1102 58-59 0.2930325136340741 1102 60-61 0.18470160864099228 1102 62-63 0.21436555189296058 1102 64-65 0.18777509217584054 1102 66-67 0.11432687003687647 1102 68-69 0.10063159884259676 1102 70-71 0.07602697250682411 1102 72-73 0.22849921596545641 1102 74-75 0.20320683788024496 1102 76-77 0.19453621958133027 1102 78-79 0.2451261112686467 1102 80-81 0.19668216798984872 1102 82-83 0.19655638869296155 1102 84-85 0.20817404755430857 1102 86-87 0.2010942983680195 1102 88-89 0.13280822523914537 1102 90-91 0.14265150400935767 1102 92-93 0.24452171811041978 1102 94-95 0.176245778862814 1102 96-97 0.16409997659119568 1102 98-99 0.1554407307807253 1102 100-101 0.2202811613623119 1102 102-103 0.275751968276996 1102 104-105 0.21944394714373416 1102 106-107 0.17155518868223396 1102 108-109 0.31037868728861184 1102 110-111 0.1605418880052838 1102 112-113 0.11215945368037339 1102 114-115 0.022313352784141927 1102 116-117 -0.004972303842841086 1102 118-119 -0.12512021532925388 1102 120-121 -0.07743547892774316 1102 122-123 -0.05370629758792589 1102 124-125 -0.07681301116992145 1103 1 0.13160452810223688 1103 2 0.17162648184736184 1103 3 0.21002627743596491 1103 4 0.12583706762612934 1103 5 -0.04177838735436268 1103 6 0.03869721310555008 1103 7 0.12264004611348156 1103 8 0.15080756711676457 1103 9 0.25619686909440276 1103 10-11 0.2598440873433461 1103 12-13 0.23291041886280084 1103 14-15 0.34225815581864083 1103 16-17 0.30808053120853884 1103 18-19 0.3387696585654041 1103 20-21 0.23403580571020655 1103 22-23 0.230366908206868 1103 24-25 0.24334081526021833 1103 26-27 0.25471723243446576 1103 28-29 0.24392318749877973 1103 30-31 0.30193003138561636 1103 32-33 0.293097489954377 1103 34-35 0.28859995562883256 1103 36-37 0.21163132917438787 1103 38-39 0.26285457134843426 1103 40-41 0.20552147472615445 1103 42-43 0.17336195761987483 1103 44-45 0.17607697936674072 1103 46-47 0.3458737012624482 1103 48-49 0.3405446162537302 1103 50-51 0.14175009992217014 1103 52-53 0.2191455176048862 1103 54-55 0.29454056277842966 1103 56-57 0.2717026546826844 1103 58-59 0.16648456237783904 1103 60-61 0.31981512404963297 1103 62-63 0.35904080700215957 1103 64-65 0.2771400818673513 1103 66-67 0.17524355897183597 1103 68-69 0.26495155371219425 1103 70-71 0.3198296766704374 1103 72-73 0.3212837609988526 1103 74-75 0.42915657539912644 1103 76-77 0.3410649285939513 1103 78-79 0.4174713954547542 1103 80-81 0.3703012187464978 1103 82-83 0.41222641207568955 1103 84-85 0.3747997364986162 1103 86-87 0.3279030631672839 1103 88-89 0.2592665949236803 1103 90-91 0.352574986501601 1103 92-93 0.28967276219837856 1103 94-95 0.39076059652923334 1103 96-97 0.3051656332739441 1103 98-99 0.3337344956999715 1103 100-101 0.3262083702852472 1103 102-103 0.35188908482007974 1103 104-105 0.3317995743916633 1103 106-107 0.2378090480109023 1103 108-109 0.31727951876015226 1103 110-111 0.2504813792633058 1103 112-113 0.3033698440851964 1103 114-115 0.26725143473389323 1103 116-117 0.24811881963005433 1103 118-119 0.17360809240673092 1103 120-121 0.2679837404578329 1103 122-123 0.11773869688669336 1103 124-125 -0.006938368428549779 1104 1 0.10592719866618694 1104 2 0.041006035793380136 1104 3 0.09615912012046124 1104 4 0.12864422308384604 1104 5 0.20853181741932758 1104 6 0.18970943998033363 1104 7 0.20292457051551338 1104 8 0.2026800114005809 1104 9 0.23641697961207342 1104 10-11 0.26891648684571834 1104 12-13 0.22683369089342165 1104 14-15 0.23428042728421872 1104 16-17 0.29148219274603093 1104 18-19 0.3042078372587653 1104 20-21 0.1848401071623158 1104 22-23 0.17924173393851817 1104 24-25 0.29078186418435337 1104 26-27 0.2238341618878792 1104 28-29 0.2548777055364937 1104 30-31 0.3377795219358788 1104 32-33 0.31445355230280114 1104 34-35 0.36731013249524835 1104 36-37 0.39267500852142945 1104 38-39 0.3371448118742535 1104 40-41 0.2505297637546633 1104 42-43 0.24441436610287326 1104 44-45 0.25855362426329975 1104 46-47 0.31721510012271636 1104 48-49 0.32304395609254044 1104 50-51 0.16710323038422104 1104 52-53 0.20758235326064778 1104 54-55 0.2434208645015019 1104 56-57 0.19210455594691211 1104 58-59 0.1654571041342976 1104 60-61 0.19891243322857122 1104 62-63 0.1528893013276118 1104 64-65 0.1370640183182701 1104 66-67 0.04423208656697142 1104 68-69 0.021805669649886283 1104 70-71 0.012386120959121172 1104 72-73 0.13618105370925093 1104 74-75 0.19823117746693697 1104 76-77 0.27101128010311726 1104 78-79 0.1792561346373347 1104 80-81 0.23492882901978618 1104 82-83 0.18469704149425326 1104 84-85 0.2106277895565114 1104 86-87 0.21732058804349919 1104 88-89 0.27761613516349826 1104 90-91 0.2817255953304425 1104 92-93 0.2635815897805003 1104 94-95 0.2600348897511324 1104 96-97 0.2501556487162375 1104 98-99 0.2565353882460073 1104 100-101 0.25769874856267805 1104 102-103 0.07725117968290718 1104 104-105 0.08931740077190753 1104 106-107 0.11631738388955881 1104 108-109 0.27836621100578185 1104 110-111 0.2902831159232804 1104 112-113 0.1805930459014462 1104 114-115 0.14743075925615656 1104 116-117 0.04479677900793533 1104 118-119 -0.007946882288109691 1104 120-121 0.04484925862656297 1104 122-123 -0.13148857987843243 1104 124-125 -0.326299405410964 1105 1 0.04560084713579471 1105 2 0.0612464858824211 1105 3 0.039026034508644614 1105 4 -0.022905268840553106 1105 5 0.0013897965786711097 1105 6 0.023025519078679224 1105 7 0.07467384183220105 1105 8 0.08818214044993056 1105 9 0.039683576029361234 1105 10-11 0.03704021203505192 1105 12-13 0.03827478924872452 1105 14-15 0.10339657909683808 1105 16-17 0.10220354301316092 1105 18-19 0.07372545814624942 1105 20-21 0.019619098898772336 1105 22-23 0.05627881205706586 1105 24-25 0.12764029934847798 1105 26-27 0.03985749207847533 1105 28-29 0.026630674189242143 1105 30-31 0.13604731998738373 1105 32-33 0.12202107490276859 1105 34-35 0.1299482434029997 1105 36-37 0.07899363261943648 1105 38-39 0.11056034931286263 1105 40-41 0.16049901424301538 1105 42-43 0.12650477829421902 1105 44-45 0.14975951042232793 1105 46-47 0.11544684914753844 1105 48-49 0.11523056064396542 1105 50-51 0.2130669718418332 1105 52-53 0.1740530611156359 1105 54-55 0.09937111068246196 1105 56-57 0.06846101611736088 1105 58-59 -0.061556249411133024 1105 60-61 -0.023247242391811085 1105 62-63 0.1093325256511477 1105 64-65 0.1280893512325676 1105 66-67 0.11818219717894607 1105 68-69 -0.11327927664630977 1105 70-71 -0.012269256508076865 1105 72-73 0.012747953924318267 1105 74-75 0.015432417306968205 1105 76-77 0.1639342707550142 1105 78-79 0.2220349291825272 1105 80-81 0.14652213437661032 1105 82-83 0.09833035202735374 1105 84-85 0.07687450046796585 1105 86-87 0.10768348157870378 1105 88-89 0.18436716271481401 1105 90-91 0.18238772659820057 1105 92-93 0.2372544001872683 1105 94-95 0.14385565522294286 1105 96-97 0.2123383710647886 1105 98-99 0.22607981481999673 1105 100-101 0.1346387716094064 1105 102-103 0.2252737211084792 1105 104-105 0.07719321957779357 1105 106-107 0.1783229422181165 1105 108-109 0.21642289116771707 1105 110-111 0.16916454851776308 1105 112-113 0.1478555998979587 1105 114-115 0.007079573951834561 1105 116-117 0.08173316634085737 1105 118-119 -0.08751447883479813 1105 120-121 0.022667090878425 1105 122-123 0.06357455213494845 1105 124-125 -0.14188737427939202 1106 1 0.09346920366612466 1106 2 0.054410182276214414 1106 3 0.002764715552977748 1106 4 0.014414857858554342 1106 5 0.10045744398431111 1106 6 -0.018595302513844558 1106 7 0.08437022749194512 1106 8 0.04045569018014561 1106 9 0.15163948343654 1106 10-11 0.1618656754667427 1106 12-13 0.1441980541498964 1106 14-15 0.21741703962128156 1106 16-17 0.14796058648364152 1106 18-19 0.14417785042557085 1106 20-21 0.026365542093572003 1106 22-23 0.051272042030596765 1106 24-25 0.10726963531663358 1106 26-27 0.06706933901488554 1106 28-29 0.09916388391368258 1106 30-31 0.1724924258478211 1106 32-33 0.15046941736445518 1106 34-35 0.1886209929282856 1106 36-37 0.29821928797019126 1106 38-39 0.1853943515166776 1106 40-41 0.0884685755534278 1106 42-43 0.20613899356072807 1106 44-45 0.32017103857791085 1106 46-47 0.18832018662828176 1106 48-49 0.2011580542703797 1106 50-51 0.06909473504948238 1106 52-53 -0.009815978516463986 1106 54-55 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-0.8312340176995221 2216 42-43 -0.619051974917646 2216 44-45 -0.5564873718922456 2216 46-47 -0.7143423517133769 2216 48-49 -0.6009440785766103 2216 50-51 -0.4559196016169409 2216 52-53 -0.5455195499960581 2216 54-55 -0.4795721727330218 2216 56-57 -0.4489718598972985 2216 58-59 -0.4145785293069082 2216 60-61 -0.44902923638492354 2216 62-63 -0.3888521529525448 2216 64-65 -0.29911169107725755 2216 66-67 -0.24781994464914447 2216 68-69 -0.48392698778555854 2216 70-71 -0.6155263002969491 2216 72-73 -0.6562431543496245 2216 74-75 -0.66090177764125 2216 76-77 -0.6451233248859793 2216 78-79 -0.5545308901713639 2216 80-81 -0.5273786089842432 2216 82-83 -0.5728092121420261 2216 84-85 -0.5642144133042706 2216 86-87 -0.4793877603676364 2216 88-89 -0.45147357457636517 2216 90-91 -0.43461207456759965 2216 92-93 -0.43991245659280054 2216 94-95 -0.4911185835385652 2216 96-97 -0.52675784027155 2216 98-99 -0.5041529258478796 2216 100-101 -0.4401213192264315 2216 102-103 -0.5037660143373799 2216 104-105 -0.49594288417877763 2216 106-107 -0.3850242397430854 2216 108-109 -0.3646695458070681 2216 110-111 -0.3211989692430173 2216 112-113 -0.28911517845074286 2216 114-115 -0.24837458778758403 2216 116-117 -0.32934172438753606 2216 118-119 -0.2739613116152313 2216 120-121 -0.25731658378695954 2216 122-123 -0.23943429773126823 2216 124-125 -0.17984395167997036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 258.0 3 9.0 4 22.0 5 123.0 6 272.0 7 604.0 8 975.0 9 1697.0 10 2906.0 11 3771.0 12 5023.0 13 7034.0 14 10909.0 15 9403.0 16 4639.0 17 4140.0 18 4237.0 19 3899.0 20 3674.0 21 3743.0 22 3879.0 23 4434.0 24 5504.0 25 7401.0 26 9826.0 27 12973.0 28 16687.0 29 21333.0 30 26563.0 31 33922.0 32 43019.0 33 56129.0 34 76451.0 35 117652.0 36 238701.0 37 506587.0 38 247113.0 39 1076.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07864000775305 18.64248070927058 13.206054064169873 24.0728252188065 2 16.211943433997867 20.59604914645848 38.22889131811828 24.963116101425374 3 18.597569939088114 25.07276551729668 30.221477119955527 26.108187423659686 4 12.785349074027053 16.815649998529988 36.82015357600088 33.57884735144208 5 13.028581208512255 38.594960598967894 34.40415089722839 13.972307295291465 6 32.104528391916645 37.00321914727682 16.90015564446767 13.992096816338858 7 28.250861292486647 33.14532790587656 20.912168211959468 17.69164258967732 8 27.134722448663222 34.48464106353919 19.705155994836254 18.67548049296132 9 27.20588431819579 14.917064683132567 19.341796138950734 38.535254859720915 10-11 25.043331898959497 26.31308683485368 27.722459354211043 20.921121911975774 12-13 25.922966106904504 23.902704017404925 28.002262479720535 22.172067395970032 14-15 22.784594023204782 25.01249508882872 25.90041481022165 26.30249607774484 16-17 22.243997680056236 28.537279465023108 26.73300868375264 22.485714171168016 18-19 22.374694972831534 26.545148030052356 29.117900183617667 21.96225681349844 20-21 22.946608445226968 25.766829147692917 29.247353140650212 22.039209266429904 22-23 22.860165924088662 25.89824320387441 29.08292061676293 22.158670255273996 24-25 22.59888666679585 26.097146373715663 29.085913481808944 22.218053477679547 26-27 22.241447511730062 26.36865203510399 28.91846220253885 22.471438250627095 28-29 22.26455576562704 26.43269105804866 29.062920437624307 22.23983273869999 30-31 22.29568958086521 25.94109381170306 29.15414647901252 22.609070128419205 32-33 22.11010400344785 26.179234724389193 29.01669467487647 22.69396659728649 34-35 22.814245121802507 26.153787044817108 28.6541169631477 22.37785087023268 36-37 22.91428903612751 25.80285957123804 28.57149061732421 22.711360775310236 38-39 22.45597986887507 26.017347459688306 28.737367932924762 22.789304738511866 40-41 22.70124984548155 25.87347193777834 28.803542732287173 22.621735484452937 42-43 22.70193934469607 25.775898243203876 28.61642616404782 22.905736248052236 44-45 22.613123415350085 26.04573948213415 28.609276656279683 22.73186044623608 46-47 23.161852159712627 26.429351297751953 27.68460658511227 22.724189957423153 48-49 23.036644142022194 26.463984957733715 27.487850657130274 23.011520243113814 50-51 22.99082980753554 26.52623835016718 27.60926854952733 22.873663292769955 52-53 23.32452217978495 26.26567899782706 27.575859221108278 22.83393960127971 54-55 22.784393567234268 26.43185699738338 27.675719703752804 23.108029731629546 56-57 22.90410124126051 26.71868960201686 27.556230574505197 22.82097858221743 58-59 22.915997782953262 26.111906068848057 27.59702001656437 23.37507613163431 60-61 22.56583727981157 26.277481269888998 28.141455143785443 23.015226306513988 62-63 22.621823775147202 26.360093759939275 28.00704001368446 23.011042451229063 64-65 22.37232291051378 26.326083063608692 27.879316151138454 23.422277874739073 66-67 22.34302292949028 26.551662849094072 27.773441989378505 23.33187223203714 68-69 22.54098826195871 26.178297686669737 27.955775374234843 23.32493867713671 70-71 22.48989785094423 26.321080458042047 27.690763152839377 23.498258538174348 72-73 22.541051794585673 26.372341029744383 27.418856930946124 23.66775024472382 74-75 22.58103098514755 26.64861672016614 27.350179207637638 23.420173087048674 76-77 22.768865836444576 26.388968236070394 27.40872150809759 23.43344441938744 78-79 22.79622046949461 26.497773602354158 27.39197427748986 23.31403165066137 80-81 22.798913125186047 26.732536652624695 27.092355961190297 23.37619426099896 82-83 22.796721609420896 26.61951719511315 27.16769745581282 23.416063739653133 84-85 22.629448662373566 26.482480977557277 27.21942419003232 23.668646170036833 86-87 22.839385321812014 26.338945655050022 27.139900894568175 23.681768128569786 88-89 22.763947058241815 26.578724405113498 26.97816633569159 23.6791622009531 90-91 22.650964277063654 26.7522279853333 26.987997694755567 23.608810042847477 92-93 22.744703285072447 26.165517831226765 27.228502433535485 23.86127645016531 94-95 22.56559587541795 26.00608851601109 27.323652200872917 24.104663407698045 96-97 22.828861383360017 25.959048181597073 27.165559258794005 24.04653117624891 98-99 22.67221708088749 26.041955518730976 26.944543744591854 24.34128365578968 100-101 22.40917339975999 25.926273630417988 27.274005938842222 24.390547030979803 102-103 22.38128408796679 25.63648968069024 27.31236897274633 24.669857258596643 104-105 22.324036314967596 25.76078814732058 27.223296157287347 24.691879380424474 106-107 22.555755194986993 25.840094829101147 26.907991316241553 24.696158659670303 108-109 22.540264612010745 25.693645162249506 26.849389119174965 24.916701106564783 110-111 22.48205266644905 25.908776393948273 27.076476505840336 24.532694433762337 112-113 22.417392094551072 25.977556949541224 27.166494719989736 24.438556235917968 114-115 22.23397488153052 26.090614116911265 26.750414943858964 24.924996057699246 116-117 22.130714041110192 25.96814419470963 27.04405856657229 24.857083197607892 118-119 22.00171323036133 26.226723720890455 26.6394291548509 25.13213389389732 120-121 22.002513732987033 26.33005006752066 26.827217273528493 24.840218925963814 122-123 21.926766115964785 26.363763927634764 26.73294021348746 24.976529742912984 124-125 21.93962727847672 26.362392016766158 26.6198445596028 25.07813614515432 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 74.0 1 74.5 2 140.0 3 226.5 4 162.0 5 123.0 6 299.0 7 737.5 8 1330.0 9 1620.0 10 1295.5 11 897.0 12 850.0 13 963.5 14 1083.0 15 1163.5 16 1300.5 17 1421.0 18 1539.5 19 1707.0 20 1890.0 21 2181.5 22 2614.0 23 3204.0 24 3987.0 25 4954.0 26 6417.0 27 8523.5 28 10512.0 29 12926.0 30 15841.5 31 18982.0 32 23327.5 33 25764.0 34 26816.0 35 28338.0 36 31190.5 37 34934.0 38 37395.5 39 39487.0 40 41203.5 41 44474.0 42 47735.0 43 49169.0 44 52078.5 45 56514.5 46 58287.5 47 58258.5 48 57170.5 49 55403.5 50 55253.0 51 54264.0 52 53525.0 53 54293.5 54 55388.0 55 52623.0 56 47120.5 57 43096.5 58 39841.0 59 34968.0 60 28064.0 61 22987.0 62 19303.0 63 14799.0 64 11205.5 65 8922.0 66 6678.5 67 5486.5 68 4054.5 69 2408.5 70 1630.5 71 1172.5 72 894.0 73 656.0 74 532.0 75 468.0 76 261.5 77 84.0 78 31.5 79 13.5 80 5.5 81 2.5 82 2.5 83 1.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026927918705749343 2 0.0 3 0.0 4 0.0 5 0.05445720532304148 6 0.015301472415922085 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.004559705142631E-4 22-23 0.0 24-25 6.68186568380877E-5 26-27 4.009119410285262E-4 28-29 5.011399262856577E-4 30-31 3.3409328419043853E-4 32-33 2.004559705142631E-4 34-35 3.6750261260948237E-4 36-37 0.0 38-39 0.0 40-41 2.004559705142631E-4 42-43 0.0 44-45 2.3386529893330697E-4 46-47 0.0 48-49 3.3409328419043853E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 3.006839557713947E-4 58-59 1.6704664209521927E-4 60-61 3.340932841904385E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 6.68186568380877E-5 70-71 3.340932841904385E-5 72-73 2.004559705142631E-4 74-75 0.0 76-77 3.340932841904385E-5 78-79 0.0 80-81 2.3386529893330697E-4 82-83 0.0 84-85 3.340932841904385E-5 86-87 0.0 88-89 0.0 90-91 3.340932841904385E-5 92-93 0.0 94-95 0.0 96-97 0.0 98-99 2.004559705142631E-4 100-101 0.0 102-103 3.340932841904385E-5 104-105 0.0010022798525713154 106-107 6.68186568380877E-5 108-109 0.0013029638083427103 110-111 0.003775254111351955 112-113 0.0 114-115 0.0 116-117 3.340932841904385E-5 118-119 0.0 120-121 6.013679115427894E-4 122-123 8.686425388951401E-4 124-125 1.0022798525713155E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1496588.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.66766705675214 #Duplication Level Percentage of deduplicated Percentage of total 1 83.29470190812233 58.86242262034607 2 10.519529205247146 14.867811749403714 3 3.0111286460961195 6.3836830988210815 4 1.2230843771945465 3.4573007819959707 5 0.6120640994764002 2.1626570999594534 6 0.34467692243506426 1.4614508398072261 7 0.21681106560458213 1.0725072538854978 8 0.1485202550879434 0.8396463950190944 9 0.09976102386774889 0.6344890937934105 >10 0.48542228356129935 6.045305736337085 >50 0.031890738828045224 1.4606901196958555 >100 0.011547705440480917 1.4150540100207525 >500 3.4470761530296394E-4 0.18208863035142248 >1k 5.17061422954446E-4 1.1548925705633364 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3816 0.2549799944941427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3596 0.24027988998976338 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2070 0.13831461965484154 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.68186568380877E-5 0.0 12-13 0.0 0.0 0.0 6.68186568380877E-5 0.0 14-15 0.0 0.0 0.0 6.68186568380877E-5 0.0 16-17 0.0 0.0 0.0 6.68186568380877E-5 0.0 18-19 0.0 0.0 0.0 6.68186568380877E-5 0.0 20-21 0.0 0.0 0.0 6.68186568380877E-5 0.0 22-23 0.0 0.0 0.0 2.3386529893330697E-4 0.0 24-25 0.0 0.0 0.0 4.3432126944757004E-4 0.0 26-27 0.0 0.0 0.0 9.354611957332279E-4 0.0 28-29 0.0 0.0 0.0 0.0030402488861329905 0.0 30-31 0.0 0.0 0.0 0.010457119795160726 0.0 32-33 0.0 0.0 0.0 0.02836451982776823 0.0 34-35 0.0 0.0 0.0 0.05372220009782251 0.0 36-37 0.0 0.0 0.0 0.09802296958147466 0.0 38-39 0.0 0.0 0.0 0.16758119134992394 0.0 40-41 0.0 0.0 0.0 0.24592606649258178 0.0 42-43 0.0 0.0 0.0 0.3215647860332971 0.0 44-45 0.0 0.0 0.0 0.41106837686791553 0.0 46-47 0.0 0.0 0.0 0.5261969225999407 0.0 48-49 0.0 0.0 0.0 0.6494439351377934 0.0 50-51 0.0 0.0 0.0 0.7912665342766345 0.0 52-53 0.0 0.0 0.0 0.9231999722034387 0.0 54-55 0.0 0.0 0.0 1.0610134519319947 0.0 56-57 0.0 0.0 0.0 1.2220130055833671 0.0 58-59 0.0 0.0 0.0 1.4037597521829654 0.0 60-61 0.0 0.0 0.0 1.6061534637455332 0.0 62-63 0.0 0.0 0.0 1.8051060144809394 0.0 64-65 0.0 0.0 0.0 2.004760161113145 0.0 66-67 0.0 0.0 0.0 2.2434698126672137 0.0 68-69 0.0 0.0 0.0 2.5116130825584597 0.0 70-71 0.0 0.0 0.0 2.795458736806656 0.0 72-73 0.0 0.0 0.0 3.095842008622279 0.0 74-75 0.0 0.0 0.0 3.383997466236533 0.0 76-77 0.0 0.0 0.0 3.69333443806846 0.0 78-79 0.0 0.0 0.0 4.051916759990058 0.0 80-81 0.0 0.0 0.0 4.440934980101404 0.0 82-83 0.0 0.0 0.0 4.855244061825967 0.0 84-85 0.0 0.0 0.0 5.271424065941996 0.0 86-87 0.0 0.0 0.0 5.674641250631437 0.0 88-89 0.0 0.0 0.0 6.116579846958548 0.0 90-91 0.0 0.0 0.0 6.611605866143521 0.0 92-93 0.0 0.0 0.0 7.123904508121139 0.0 94-95 0.0 0.0 0.0 7.6317597094190255 0.0 96-97 0.0 0.0 0.0 8.1371426204139 0.0 98-99 0.0 0.0 0.0 8.668150486306185 0.0 100-101 0.0 0.0 0.0 9.25739081163286 0.0 102-103 0.0 0.0 0.0 9.894439885927188 0.0 104-105 0.0 0.0 0.0 10.529150307232184 0.0 106-107 0.0 0.0 0.0 11.14184398110903 0.0 108-109 0.0 0.0 0.0 11.769839127401795 0.0 110-111 0.0 0.0 0.0 12.445943706617987 0.0 112-113 0.0 0.0 0.0 13.162339935907543 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2885 0.0 73.08056 1 GTATCAA 4950 0.0 56.309944 1 ATCAACG 6465 0.0 43.00783 3 TCAACGC 6515 0.0 42.769154 4 CAACGCA 6640 0.0 41.874344 5 TATCAAC 6670 0.0 41.77527 2 AACGCAG 6925 0.0 40.216805 6 GTACATG 7070 0.0 36.560722 1 ACGCAGA 7980 0.0 34.967484 7 CGCAGAG 8085 0.0 34.51336 8 TACATGG 7490 0.0 34.101597 2 ACATGGG 7370 0.0 33.848995 3 GTGGTAT 1065 0.0 31.876432 1 GCAGAGT 9120 0.0 30.270357 9 CATGGGA 4860 0.0 29.891848 4 ACGTGCG 80 0.0053457045 29.769339 5 CAGAGTA 8645 0.0 27.666578 10-11 GAGTACT 5530 0.0 27.650463 12-13 CATGGGG 3170 0.0 27.609423 4 TAAGGTG 805 0.0 27.365604 5 >>END_MODULE