FastQCFastQC Report
Thu 26 May 2016
SRR1364351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741464
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15200.046.2273671
GTATCAA22950.043.849511
ATCAACG22200.040.755773
CAACGCA22300.040.307245
TCAACGC23450.038.583294
GTACATG58750.035.5762371
AACGCAG25450.035.548146
TACATGG59600.033.957142
ACATGGG58800.033.912983
TATCAAC27200.033.4827422
ACGCAGA28150.032.1265537
CGCAGAG29450.030.706648
CATGGGG39750.030.2491194
TAAGGTA6400.025.1120594
TAAGGTG11750.024.8238545
AGGTAAG11550.023.1915112
ATGGGGA23600.022.4485875
GAGTACA43150.022.2180421
GGTAAGG12500.021.9051553
GTAAGGT11700.021.8766654
Produced by FastQC (version 0.11.5)