FastQCFastQC Report
Thu 26 May 2016
SRR1364351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741464
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21370.12271284390604686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.11811900791517942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24050.064.624771
GTATCAA39950.049.487431
ATCAACG48100.040.2287943
CAACGCA48450.039.8152965
TCAACGC48750.039.692414
AACGCAG49850.038.679336
TATCAAC51550.037.5364722
CCGTTGC2850.033.4021539
ACGCAGA57850.033.2199137
CGCAGAG59350.032.380328
GTACATG55700.031.4315451
TCCGTTG2700.030.8506018
TACATGG57700.030.2336462
ACATGGG56350.029.5844043
TATTCCG3450.029.337875
GTGGTAT14350.027.803311
TAAGGTG11250.027.5200715
ATTCCGT3800.026.6234596
TGGTATC13350.026.3129442
GCAGAGT73750.026.0579269
Produced by FastQC (version 0.11.5)