FastQCFastQC Report
Thu 26 May 2016
SRR1364350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699977
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15700.047.405871
GTATCAA22700.046.6891441
ATCAACG24300.041.63883
CAACGCA24400.041.468155
TCAACGC24450.041.1399154
AACGCAG26000.038.682796
GTACATG64100.035.483551
TATCAAC29550.034.4424672
TACATGG66850.032.5863152
ACATGGG65300.032.3571853
ACGCAGA32950.030.515527
CGCAGAG33900.029.4831248
GGACCGT2154.0290615E-927.6799956
CATGGGG37350.027.0903054
CATGGGA36350.025.8707054
TAAGGTG9150.024.7182715
GTAAGGT8850.024.2111174
GAGTACA51450.023.8398531
GGTAAGG8700.023.2602983
GCAGAGT43650.022.7612519
Produced by FastQC (version 0.11.5)