FastQCFastQC Report
Thu 26 May 2016
SRR1364350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699977
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24780.14576667801976145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.1445313671890855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25550.067.84771
GTATCAA43450.052.3729061
ATCAACG51300.043.298323
CAACGCA52650.042.414325
TCAACGC52700.042.374084
AACGCAG54800.040.9410746
TATCAAC57000.038.968492
ACGCAGA65400.034.3882687
GTACATG63900.033.4677661
CGCAGAG67550.033.0295148
ACATGGG64850.030.9456443
TACATGG67550.030.9429262
GCAGAGT78150.028.6256339
CATGGGG36700.025.4749434
GAGTACT45000.025.18713212-13
TATCACG1200.001281862124.8123782
TATAACG1200.001281862124.8123782
CAGAGTA78600.024.56339610-11
CATGGGA37600.024.0732884
GTACTTT47300.023.83660114-15
Produced by FastQC (version 0.11.5)