FastQCFastQC Report
Thu 26 May 2016
SRR1364349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312035
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.067.9766541
GTATCAA34250.054.0475771
TCAACGC41950.043.1288954
ATCAACG42400.043.092263
GTACATG107650.041.303131
CAACGCA43850.041.1228525
TATCAAC45300.040.333592
AACGCAG44900.040.159656
TACATGG110900.038.639162
ACATGGG112750.036.6327633
CATGGGA94850.034.6360554
ATGGGAG36000.034.054535
ACGCAGA55600.032.641427
CGCAGAG55600.032.6376888
ATGGGAT36250.029.7153425
GCAGAGT62400.028.5089289
CATGGGG22050.027.2608594
TATAGGG2006.102164E-826.7817692
GTATAGA5850.025.4366861
TGGTATC8200.025.4027612
Produced by FastQC (version 0.11.5)