##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364349_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1312035 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.017227436768074 33.0 31.0 34.0 27.0 34.0 2 30.98519018166436 33.0 31.0 34.0 27.0 34.0 3 31.062380195650267 33.0 31.0 34.0 27.0 34.0 4 34.36290876386682 37.0 35.0 37.0 30.0 37.0 5 34.50770901690885 37.0 35.0 37.0 31.0 37.0 6 34.52725498938672 37.0 35.0 37.0 31.0 37.0 7 34.43640527882259 37.0 35.0 37.0 31.0 37.0 8 34.364703685496195 37.0 35.0 37.0 30.0 37.0 9 35.81979291710968 39.0 37.0 39.0 30.0 39.0 10-11 35.778420926271025 39.0 37.0 39.0 30.0 39.0 12-13 35.818309724969225 39.0 37.0 39.0 30.0 39.0 14-15 37.13263251361435 40.0 38.0 41.0 31.0 41.0 16-17 37.18473097135366 40.0 38.0 41.0 31.0 41.0 18-19 37.20562713647121 40.0 38.0 41.0 31.0 41.0 20-21 37.24300609358744 40.0 38.0 41.0 31.0 41.0 22-23 37.23799441325879 40.0 38.0 41.0 30.5 41.0 24-25 37.294212425735594 40.0 38.0 41.0 30.0 41.0 26-27 37.29910977984581 40.0 38.0 41.0 30.0 41.0 28-29 37.32087558639823 40.0 38.0 41.0 30.0 41.0 30-31 37.241254234833676 40.0 38.0 41.0 30.0 41.0 32-33 37.2055836925082 40.0 38.0 41.0 30.0 41.0 34-35 37.19492772677558 40.0 38.0 41.0 30.0 41.0 36-37 37.13466218507891 40.0 37.0 41.0 30.0 41.0 38-39 37.05525119375626 40.0 37.0 41.0 30.0 41.0 40-41 36.98754263415229 40.0 37.0 41.0 30.0 41.0 42-43 36.8668976056279 40.0 37.0 41.0 30.0 41.0 44-45 36.764639281726474 40.0 37.0 41.0 30.0 41.0 46-47 36.667472285419215 40.0 36.5 41.0 30.0 41.0 48-49 36.53750471595651 40.0 36.0 41.0 29.0 41.0 50-51 36.44016851684597 40.0 36.0 41.0 29.0 41.0 52-53 36.30052590060478 40.0 36.0 41.0 29.0 41.0 54-55 36.131893585155886 40.0 35.0 41.0 29.0 41.0 56-57 35.963040239018014 39.0 35.0 41.0 29.0 41.0 58-59 35.765744054083925 39.0 35.0 41.0 28.5 41.0 60-61 35.53299835751333 39.0 35.0 41.0 28.0 41.0 62-63 35.287482041256524 38.5 35.0 40.5 28.0 41.0 64-65 34.961735014690916 38.0 34.0 40.0 28.0 41.0 66-67 34.65510790489583 37.0 34.0 40.0 27.5 41.0 68-69 34.32883535881284 37.0 34.0 40.0 27.0 41.0 70-71 33.96858353626237 36.0 34.0 39.0 26.0 41.0 72-73 33.591983064476175 36.0 33.5 39.0 26.0 40.5 74-75 33.237707835537925 35.0 33.0 38.5 26.0 40.0 76-77 32.9835766576349 35.0 33.0 37.0 26.0 39.0 78-79 32.79609575964056 35.0 33.0 37.0 26.0 39.0 80-81 32.500602880258526 35.0 33.0 36.5 26.0 39.0 82-83 32.190226251586274 35.0 33.0 36.0 25.5 37.5 84-85 31.9180856455811 35.0 33.0 36.0 25.0 37.0 86-87 31.688336439195602 35.0 33.0 35.0 25.0 37.0 88-89 31.414072795314148 35.0 33.0 35.0 24.0 36.0 90-91 31.281178474659594 35.0 33.0 35.0 24.0 36.0 92-93 31.125409383133835 35.0 32.0 35.0 23.0 36.0 94-95 31.004749111113654 35.0 32.0 35.0 22.5 36.0 96-97 30.898410484476408 35.0 32.0 35.0 20.0 35.0 98-99 30.757491987637525 35.0 32.0 35.0 20.0 35.0 100-101 30.663432377947235 35.0 32.0 35.0 19.0 35.0 102-103 29.68981772589908 34.0 30.0 35.0 17.5 35.0 104-105 30.348677436196443 34.5 31.5 35.0 18.0 35.0 106-107 30.661112698975256 35.0 32.0 35.0 19.0 35.0 108-109 30.68509414764088 35.0 33.0 35.0 18.0 35.0 110-111 30.613519456416938 35.0 33.0 35.0 17.0 35.0 112-113 30.515975564676246 35.0 32.0 35.0 12.5 35.0 114-115 30.40401551787871 35.0 32.0 35.0 6.0 35.0 116-117 30.264784476023884 35.0 32.0 35.0 2.0 35.0 118-119 30.07907563441524 35.0 32.0 35.0 2.0 35.0 120-121 29.878432358892866 35.0 32.0 35.0 2.0 35.0 122-123 29.677559287671443 35.0 32.0 35.0 2.0 35.0 124-125 28.874755627708105 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23549718743191406 1101 2 0.2428167436262605 1101 3 0.27959380877369 1101 4 0.33017457103440506 1101 5 0.23703685981005407 1101 6 0.2315676753446425 1101 7 0.21049641765461047 1101 8 0.1986440335925863 1101 9 0.3331694990103671 1101 10-11 0.4285967608261103 1101 12-13 0.3595595259498836 1101 14-15 0.4506560859145452 1101 16-17 0.4846851444021638 1101 18-19 0.31104206827815517 1101 20-21 0.4092383997523612 1101 22-23 0.42189441053481147 1101 24-25 0.48505320944462227 1101 26-27 0.563934933408369 1101 28-29 0.5011286520404568 1101 30-31 0.4058509725370456 1101 32-33 0.4241898211501578 1101 34-35 0.4143270020801779 1101 36-37 0.4254171243559597 1101 38-39 0.4830117257430615 1101 40-41 0.36647518335487916 1101 42-43 0.44139343084465565 1101 44-45 0.4456436431323212 1101 46-47 0.3262581029842764 1101 48-49 0.24084833790962534 1101 50-51 0.24788448529677964 1101 52-53 0.18308738087754506 1101 54-55 0.26766262656673234 1101 56-57 0.07100204999565563 1101 58-59 0.24199702391878475 1101 60-61 0.15031008776799837 1101 62-63 0.17343488520911166 1101 64-65 0.22212284160002582 1101 66-67 0.20071465761969876 1101 68-69 0.22867587216129692 1101 70-71 0.2059061801158535 1101 72-73 0.142224816635391 1101 74-75 0.1974989563097438 1101 76-77 0.2332031300971522 1101 78-79 0.15353429320460066 1101 80-81 0.21318799300748026 1101 82-83 0.01897925151510549 1101 84-85 0.10390367015033775 1101 86-87 0.03366282001404031 1101 88-89 0.12897105028997302 1101 90-91 0.005010729255896962 1101 92-93 -0.026244899141790512 1101 94-95 -0.1450584678060558 1101 96-97 -0.2739274730767143 1101 98-99 -0.2522306757339834 1101 100-101 -0.19864442255981984 1101 102-103 -0.3555210820890622 1101 104-105 -0.18727893426149578 1101 106-107 -0.21817134786903125 1101 108-109 -0.22364256055472254 1101 110-111 -0.1949003223346928 1101 112-113 -0.29955736513647935 1101 114-115 -0.36153244320309597 1101 116-117 -0.3395041033964361 1101 118-119 -0.1882735068799768 1101 120-121 -0.39601355901078605 1101 122-123 -0.38247260229292124 1101 124-125 -0.5153776609423097 1102 1 0.24400207105538385 1102 2 0.3281638164088321 1102 3 0.299694780699177 1102 4 0.34509811078228125 1102 5 0.15311526631275285 1102 6 0.24319490137052213 1102 7 0.24928916525021805 1102 8 0.25085856851068655 1102 9 0.32019963718473576 1102 10-11 0.3271276315939815 1102 12-13 0.2469338073897731 1102 14-15 0.31063059868772314 1102 16-17 0.38867640070076703 1102 18-19 0.3083947904151074 1102 20-21 0.3751474484606163 1102 22-23 0.43953964444910554 1102 24-25 0.428747491844355 1102 26-27 0.28758663425654873 1102 28-29 0.2967837683255752 1102 30-31 0.31036300012564055 1102 32-33 0.3753902946721013 1102 34-35 0.3284975802448642 1102 36-37 0.19895517251202222 1102 38-39 0.2963630675361486 1102 40-41 0.27293003351243783 1102 42-43 0.22343636262418443 1102 44-45 0.1740869826160676 1102 46-47 0.13930706605521692 1102 48-49 0.1648170150734245 1102 50-51 0.12845088906392732 1102 52-53 0.17695696294930485 1102 54-55 0.23292406775200192 1102 56-57 0.25463877616965647 1102 58-59 0.19609216309155642 1102 60-61 0.25897817305793325 1102 62-63 0.24092147384865115 1102 64-65 0.23767684883940632 1102 66-67 0.2057175471433439 1102 68-69 0.13964897745222515 1102 70-71 0.17892319988146 1102 72-73 0.2110760374484073 1102 74-75 0.21218953446300048 1102 76-77 0.2926233342520703 1102 78-79 0.2886762997938064 1102 80-81 0.3181362629641171 1102 82-83 0.30139726431700353 1102 84-85 0.24500797952562436 1102 86-87 0.23887556065650628 1102 88-89 0.26570807964256105 1102 90-91 0.21423295176378687 1102 92-93 0.22770337852786326 1102 94-95 0.18222229190869754 1102 96-97 0.11269321831216317 1102 98-99 0.0646795079300233 1102 100-101 -0.029899812742637977 1102 102-103 -0.3061994988186427 1102 104-105 -0.09838087545576002 1102 106-107 -0.11870488848247973 1102 108-109 -0.14791147393671622 1102 110-111 -0.09086547322267435 1102 112-113 -0.1334268957881406 1102 114-115 -0.20564314266778538 1102 116-117 -0.3172416306669419 1102 118-119 -0.381322681344642 1102 120-121 -0.39525968329258276 1102 122-123 -0.4911256900553127 1102 124-125 -0.48251664974329955 1103 1 0.42127292903769487 1103 2 0.46204459526517994 1103 3 0.4377223604299125 1103 4 0.5362701668697909 1103 5 0.2973005085947804 1103 6 0.5123292879573782 1103 7 0.2631205398604166 1103 8 0.4478405441527329 1103 9 0.5486577144985887 1103 10-11 0.5414997809261024 1103 12-13 0.4745023223814755 1103 14-15 0.6086856569875962 1103 16-17 0.5619310332422529 1103 18-19 0.5308858453106708 1103 20-21 0.6321802413124544 1103 22-23 0.5134764030555417 1103 24-25 0.5746133086802843 1103 26-27 0.586521221187347 1103 28-29 0.5756904203604662 1103 30-31 0.6550503942908108 1103 32-33 0.6530061741842346 1103 34-35 0.6410359281879892 1103 36-37 0.5206325637742992 1103 38-39 0.4591210845928657 1103 40-41 0.5488968140225552 1103 42-43 0.4641201490588571 1103 44-45 0.44494592706075764 1103 46-47 0.5067845466036047 1103 48-49 0.5575293664648342 1103 50-51 0.5364676434039808 1103 52-53 0.5111836155119889 1103 54-55 0.5287039522078629 1103 56-57 0.463802889972051 1103 58-59 0.4432453437441808 1103 60-61 0.4024625688812833 1103 62-63 0.401318824514334 1103 64-65 0.43097797173314945 1103 66-67 0.4176319919201035 1103 68-69 0.4306047181530559 1103 70-71 0.4226812818135599 1103 72-73 0.4229297302866115 1103 74-75 0.4291803397533087 1103 76-77 0.44380137206891135 1103 78-79 0.45583360937908424 1103 80-81 0.5070587105030384 1103 82-83 0.44060991919320713 1103 84-85 0.4302645561291385 1103 86-87 0.4437679335117757 1103 88-89 0.5243193233144652 1103 90-91 0.4704318486625212 1103 92-93 0.389781166299624 1103 94-95 0.4498414536748889 1103 96-97 0.4968337735573911 1103 98-99 0.3954358778120195 1103 100-101 0.31329036226692963 1103 102-103 0.19675466727990454 1103 104-105 0.37432940872036724 1103 106-107 0.3681379184879958 1103 108-109 0.2852128745605924 1103 110-111 0.289995989266167 1103 112-113 0.271340042182203 1103 114-115 0.26570695783406606 1103 116-117 0.09777561628611409 1103 118-119 0.20198100907447625 1103 120-121 0.03781930742254147 1103 122-123 -0.014320422834746438 1103 124-125 -0.14825645991135872 1104 1 0.32473828117075954 1104 2 0.26042698612405957 1104 3 0.29041379560567293 1104 4 0.33819701483896125 1104 5 0.09653303764706322 1104 6 0.002417700067731232 1104 7 0.14248544959366427 1104 8 0.22382192083943409 1104 9 0.4291373880817062 1104 10-11 0.29318959668331246 1104 12-13 0.16465168886313108 1104 14-15 0.2990374507876652 1104 16-17 0.3242587438458244 1104 18-19 0.2518970376188818 1104 20-21 0.3066812127374021 1104 22-23 0.2748092229698713 1104 24-25 0.3603523323699349 1104 26-27 0.42871682817527557 1104 28-29 0.3238812968820568 1104 30-31 0.3549200887725803 1104 32-33 0.3363065141264272 1104 34-35 0.25723359111371025 1104 36-37 0.1716128095821361 1104 38-39 0.11689451979577115 1104 40-41 0.27916566351161975 1104 42-43 0.3052921693061137 1104 44-45 0.2183987999454473 1104 46-47 0.17574209114891914 1104 48-49 0.08612279063035544 1104 50-51 0.031989787143210435 1104 52-53 0.04155365038829473 1104 54-55 0.11693243610640991 1104 56-57 0.1306653400437483 1104 58-59 0.18805574059219055 1104 60-61 0.06729339505614718 1104 62-63 0.1302950794506259 1104 64-65 0.20364530788011592 1104 66-67 0.19967417257122122 1104 68-69 0.09745444583742113 1104 70-71 -0.0151790444676152 1104 72-73 0.06212862134678687 1104 74-75 0.0571141222717344 1104 76-77 -0.02074529251693491 1104 78-79 0.08670527385184812 1104 80-81 0.0869865434677024 1104 82-83 0.06167615703060392 1104 84-85 0.06877573147417593 1104 86-87 0.10709568030346972 1104 88-89 0.17045163275006558 1104 90-91 0.006015517866881481 1104 92-93 -0.050604412506800145 1104 94-95 -0.015631117988903043 1104 96-97 -0.14908381025204065 1104 98-99 -0.05652297402927786 1104 100-101 -0.04280699735707927 1104 102-103 -0.23867342126338897 1104 104-105 -0.25321422058658527 1104 106-107 -0.1716575677220682 1104 108-109 -0.08313741630145444 1104 110-111 -0.010353706753591041 1104 112-113 -0.08526239621882326 1104 114-115 -0.08166708487696894 1104 116-117 -0.17693988286120188 1104 118-119 -0.27035415967527143 1104 120-121 -0.2837733828025968 1104 122-123 -0.3039377956221827 1104 124-125 -0.39663280056826267 1105 1 0.016797197901393446 1105 2 -0.07453567407613804 1105 3 -0.1453375164133881 1105 4 0.051862517337596614 1105 5 0.05345034782582303 1105 6 0.23416593475180747 1105 7 0.0802677010302375 1105 8 0.2519636070359965 1105 9 0.3233082445670732 1105 10-11 0.2408698181972042 1105 12-13 0.2595601906788616 1105 14-15 0.31742944158277453 1105 16-17 0.30266656190347874 1105 18-19 0.305686429963842 1105 20-21 0.3467297790483741 1105 22-23 0.2621986736079549 1105 24-25 0.261292139629802 1105 26-27 0.278076959402604 1105 28-29 0.1880383934775054 1105 30-31 0.21655651587820302 1105 32-33 0.35773686485111256 1105 34-35 0.09734392441823303 1105 36-37 0.05813608192797659 1105 38-39 -0.023614375837496482 1105 40-41 0.09812990982720748 1105 42-43 0.1288902110636485 1105 44-45 0.12610925254754335 1105 46-47 0.18721092550662632 1105 48-49 0.1291605744069102 1105 50-51 0.13866494359417914 1105 52-53 0.1499369311053016 1105 54-55 0.057441670653219035 1105 56-57 0.0025844041001050755 1105 58-59 -0.05256059063479057 1105 60-61 -0.10958897313613392 1105 62-63 -0.06584054358261682 1105 64-65 -0.08954032693037561 1105 66-67 -0.05921722427790144 1105 68-69 -0.07223405872350241 1105 70-71 -0.03850095641448803 1105 72-73 0.0078033219590309955 1105 74-75 0.00357383138223355 1105 76-77 -0.08294778997084506 1105 78-79 -0.06299410983387332 1105 80-81 -0.05047509575543785 1105 82-83 -0.06803834219921612 1105 84-85 -0.059259874782849664 1105 86-87 -0.05661693117790634 1105 88-89 -0.005795272545629615 1105 90-91 -0.08141400929788745 1105 92-93 -0.11795797220048954 1105 94-95 -0.260108098133653 1105 96-97 -0.23319537381273747 1105 98-99 -0.23010517157859667 1105 100-101 -0.3796675734084296 1105 102-103 -0.3991019604951731 1105 104-105 -0.3191333287016995 1105 106-107 -0.3122338224223782 1105 108-109 -0.2555084215698642 1105 110-111 -0.2976121673065961 1105 112-113 -0.5070703813608546 1105 114-115 -0.5463815247969173 1105 116-117 -0.5127549106266933 1105 118-119 -0.5103824759019453 1105 120-121 -0.7009916312635021 1105 122-123 -0.7678388430344931 1105 124-125 -0.9088103878766312 1106 1 0.3379600928839501 1106 2 0.2985016987677369 1106 3 0.3723445564157437 1106 4 0.38632919874700633 1106 5 0.3561971044231953 1106 6 0.15011542858442795 1106 7 0.23794590389032777 1106 8 0.3444773669259149 1106 9 0.3169657953066647 1106 10-11 0.37342700773969284 1106 12-13 0.27642987714994405 1106 14-15 0.43920062812756555 1106 16-17 0.3383785440647671 1106 18-19 0.26999364407755166 1106 20-21 0.25513667250751837 1106 22-23 0.280564901678126 1106 24-25 0.3731465305089614 1106 26-27 0.3969879009722135 1106 28-29 0.4006977993451173 1106 30-31 0.5074584029488207 1106 32-33 0.48074582720797565 1106 34-35 0.5314108625970064 1106 36-37 0.5768194324611713 1106 38-39 0.5212972291553797 1106 40-41 0.5404888709555209 1106 42-43 0.5295840412272952 1106 44-45 0.42871626026290954 1106 46-47 0.4540330052156989 1106 48-49 0.3786361321738738 1106 50-51 0.19858803563801075 1106 52-53 0.2642376952706158 1106 54-55 0.26121780830126795 1106 56-57 0.30251468322929753 1106 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-0.7373839824654098 2216 112-113 -0.7397329200605434 2216 114-115 -0.6782070035264205 2216 116-117 -0.7604443957774762 2216 118-119 -0.7747500942943297 2216 120-121 -0.5789127412279562 2216 122-123 -0.5834735179570956 2216 124-125 -0.5120496952955023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22577.0 3 4643.0 4 3433.0 5 2837.0 6 3049.0 7 3216.0 8 3120.0 9 3196.0 10 3617.0 11 4289.0 12 5001.0 13 5140.0 14 5189.0 15 4688.0 16 4178.0 17 3800.0 18 3525.0 19 3301.0 20 3132.0 21 3282.0 22 4002.0 23 5624.0 24 9304.0 25 13818.0 26 15763.0 27 13669.0 28 15601.0 29 18596.0 30 21885.0 31 27087.0 32 35366.0 33 47342.0 34 67628.0 35 103877.0 36 199102.0 37 408222.0 38 209158.0 39 778.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.674123067209784 19.85609777299256 12.78863178506778 22.68114737472988 2 14.872880774560604 21.68245271427025 40.16844040557413 23.276226105595015 3 17.623547453289085 28.713588200053962 29.54460574113228 24.11825860552467 4 11.33614286378503 17.809419930287667 38.616409151782484 32.23802805414482 5 11.887496130992767 41.98356948889462 33.7727356587528 12.356198721359817 6 29.456367938912674 38.95315682902136 18.598821678193726 12.99165355387224 7 27.663837902191922 34.96392524988605 21.53273099501676 15.839505852905264 8 26.577187346374142 36.310540496252 20.293208641537763 16.819063515836085 9 27.61686997679178 16.959456112070182 19.387287686685188 36.036386224452855 10-11 23.663684903906653 29.44917862109256 27.65924446173739 19.2278920132634 12-13 24.281908812365664 27.09493757717108 27.758913736071094 20.864239874392158 14-15 21.717575709997377 27.79144828679538 25.50274212584397 24.988233877363275 16-17 21.33550147233508 31.40606761346812 26.129326925458724 21.129103988738077 18-19 21.66691437347327 29.66052734873689 27.737179267321377 20.935379010468473 20-21 22.91680481084727 28.94842744286547 27.09074071956922 21.044027026718037 22-23 22.4260023551201 29.26610189514761 26.964448356941695 21.343447392790587 24-25 21.87037693354217 29.319530347894684 27.329491972394027 21.480600746169117 26-27 21.76075467556481 29.594088262956603 26.96637434700625 21.678782714472337 28-29 21.69463919120844 29.188933344969232 27.470567589269006 21.645859874553317 30-31 21.64331994698304 28.958722837001815 27.59831498140672 21.79964223460843 32-33 21.556263023671644 29.23960049815782 27.152992986462245 22.051143491708295 34-35 22.054660668799762 29.090327753650474 27.06890585676155 21.786105720788218 36-37 22.289543453316206 28.642272745114557 26.944146666874996 22.124037134694248 38-39 21.817874868905637 28.80802449940245 27.21004518182483 22.164055449867075 40-41 21.82583477511947 28.67575436537274 27.508212464654008 21.990198394853778 42-43 21.914343955705164 28.584178749438372 27.3272300521369 22.174247242719563 44-45 21.59096842003371 28.794944583479577 27.477623219489665 22.136463776997047 46-47 22.07589736554284 28.965309614453883 26.938648740315617 22.020144279687663 48-49 21.767140358298366 28.82655569401731 27.035330612369336 22.370973335314986 50-51 21.894461656891774 28.59931328051462 27.181020323390765 22.325204739202842 52-53 22.099303288590654 28.64401560645752 27.12593777081312 22.130743334138707 54-55 21.462276734334246 28.695535525098286 27.416345401912494 22.425842338654974 56-57 21.694695644552166 28.84244703837931 27.279455197460432 22.18340211960809 58-59 21.863383909020165 28.141951347037832 27.492231486089846 22.502433257852164 60-61 21.54276552843496 28.588920676163216 27.56260287091551 22.305710924486316 62-63 21.546004180552618 28.69295803632165 27.554986461792915 22.206051321332822 64-65 21.424420233160532 28.55235212720591 27.471990591567398 22.55123704806616 66-67 21.486449303696116 28.889313177830168 27.14457099433765 22.47966652413607 68-69 21.621140613233578 28.53907328985759 27.382400556099256 22.457385540809575 70-71 21.58335261031994 28.504813403215746 27.371555246954394 22.540278739509922 72-73 21.6807031410478 28.65849313089916 27.12750814882791 22.53329557922513 74-75 21.530696591223926 28.96123925837484 27.02017247575381 22.487891674647422 76-77 21.742381311877367 28.67077915322207 27.25814878654716 22.3286907483534 78-79 21.92076615707668 28.632224405060203 27.135009270180504 22.31200016768261 80-81 21.860032621453943 29.142581667962382 26.877715278730506 22.119670431853173 82-83 22.048826664654516 28.839796986194667 26.937057031513785 22.174319317637035 84-85 21.973307785146762 28.590493894768453 27.15405625097892 22.282142069105863 86-87 22.1266943494247 28.41872915409052 27.134857409406038 22.319719087078738 88-89 22.13786627255106 28.483833815942017 27.10258718397643 22.275712727530493 90-91 21.99461064357879 28.721664625936665 27.079096103329913 22.204628627154626 92-93 22.16783198128383 28.340144075492724 27.063677739791416 22.428346203432035 94-95 21.88304222380672 28.11526191854472 27.35269761409978 22.648998243548785 96-97 22.266026184564787 28.041873962725774 27.256146475030153 22.435953377679287 98-99 22.132416381357714 28.31322959570985 26.93380019328856 22.62055382964388 100-101 21.721610102315825 28.369547322593625 27.301039011719368 22.607803563371178 102-103 22.007688165507062 27.99648480030548 27.221646983860342 22.77418005032711 104-105 21.76218642425231 28.40322600929023 27.008281482763074 22.826306083694384 106-107 21.898293147512817 28.381977320730183 27.041336273492757 22.678393258264244 108-109 22.095186193676483 28.532382627385893 26.61646370161756 22.75596747732006 110-111 21.726703477743577 28.95036602110724 26.873978908007494 22.448951593141693 112-113 21.74245094807365 28.924830554819824 26.95303553156339 22.37968296554313 114-115 21.84091192316443 28.946342463566022 26.797670573054123 22.415075040215427 116-117 21.688585877448048 29.135871160009604 26.778962130768324 22.396580831774028 118-119 21.663508500360297 29.28828784175871 26.349096205243956 22.699107452637037 120-121 21.64878554468502 29.433991028313788 26.59327466546739 22.3239487615338 122-123 21.796872588329315 29.439484382187374 26.564591901016747 22.199051128466564 124-125 21.87845489152617 29.653616219835616 26.301668790017672 22.166260098620537 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2979.0 1 2591.0 2 1960.0 3 1394.0 4 924.0 5 709.0 6 619.0 7 607.0 8 637.5 9 651.0 10 562.5 11 455.0 12 413.0 13 425.0 14 511.0 15 560.0 16 574.5 17 630.0 18 772.5 19 970.0 20 1238.0 21 1706.5 22 2265.5 23 3208.0 24 4354.5 25 5577.0 26 7494.0 27 9715.0 28 11636.5 29 14738.0 30 18800.0 31 21226.5 32 24266.5 33 27353.0 34 30159.0 35 32576.5 36 36392.0 37 41294.5 38 42901.0 39 43860.0 40 44972.5 41 46916.5 42 47621.5 43 47632.5 44 48902.0 45 50501.5 46 49125.5 47 46560.5 48 46677.0 49 45579.5 50 43328.0 51 41106.5 52 40611.0 53 40845.0 54 39238.5 55 36545.0 56 32498.5 57 28758.5 58 26929.5 59 24402.5 60 20181.0 61 16189.0 62 13222.5 63 10102.0 64 7117.0 65 5292.5 66 3910.0 67 3120.5 68 2171.0 69 1056.0 70 676.0 71 532.5 72 398.5 73 301.5 74 226.0 75 170.0 76 134.5 77 77.0 78 43.0 79 33.0 80 24.0 81 14.0 82 12.0 83 10.5 84 7.0 85 5.0 86 5.0 87 6.5 88 3.5 89 2.0 90 3.0 91 3.5 92 3.0 93 2.5 94 2.0 95 0.5 96 0.5 97 1.5 98 1.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044892095104170235 2 0.00891744503767049 3 0.00221030688967901 4 0.005487658484720301 5 0.0250755505760136 6 0.025837725365558083 7 0.002820046721314599 8 0.0 9 0.0 10-11 2.667611763405702E-4 12-13 0.0011432621843167293 14-15 4.1919613424946743E-4 16-17 2.667611763405702E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 7.621747895444862E-5 28-29 2.667611763405702E-4 30-31 1.5243495790889723E-4 32-33 7.621747895444862E-5 34-35 9.908272264078323E-4 36-37 3.810873947722431E-5 38-39 2.2865243686334585E-4 40-41 3.810873947722431E-4 42-43 6.478485711128133E-4 44-45 0.0012575884027484024 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0010670447053622808 54-55 0.0025151768054968047 56-57 0.0 58-59 6.097398316355889E-4 60-61 0.001105153444839505 62-63 5.716310921583647E-4 64-65 0.0015624583185661967 66-67 0.0027438292423601503 68-69 0.0029724816792234964 70-71 0.0016386757975206453 72-73 0.002781937981837375 74-75 0.0018673282343839913 76-77 0.001105153444839505 78-79 0.0024770680660195802 80-81 0.0011432621843167293 82-83 4.573048737266917E-4 84-85 3.429786552950188E-4 86-87 0.002057871931770113 88-89 0.0051827885689025065 90-91 3.429786552950188E-4 92-93 0.001105153444839505 94-95 0.001105153444839505 96-97 1.9054369738612156E-4 98-99 5.335223526811404E-4 100-101 0.0014481321001345237 102-103 0.0014100233606572994 104-105 8.765010079761591E-4 106-107 0.006707138147991479 108-109 0.002095980671247337 110-111 0.0015624583185661967 112-113 0.0024770680660195802 114-115 0.002934372939746272 116-117 0.0015243495790889725 118-119 0.047254836951758146 120-121 0.009908272264078321 122-123 0.004230070081971899 124-125 0.002781937981837375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1312035.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.91622967526503 #Duplication Level Percentage of deduplicated Percentage of total 1 78.64740830820836 43.976665463261256 2 11.12424995415031 12.440522308026503 3 3.967179801316308 6.6548921080042485 4 1.9068976324231903 4.265061039271769 5 1.1322185836626142 3.1654697183341005 6 0.6889099809310946 2.311274923159532 7 0.4546414803350517 1.7795286204022067 8 0.3081227696828538 1.3783250846212192 9 0.24844362722428862 1.2502827829106322 >10 1.3697562127995389 14.029496663137115 >50 0.10144777913062587 3.9077208267228754 >100 0.05072387013575967 4.84076046214867 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.810873947722431E-5 0.0 20-21 0.0 0.0 0.0 1.1432621843167293E-4 0.0 22-23 0.0 0.0 0.0 5.335223526811404E-4 0.0 24-25 0.0 0.0 0.0 0.001600567058043421 0.0 26-27 0.0 0.0 0.0 0.003163025376609618 0.0 28-29 0.0 0.0 0.0 0.00804094402969433 0.0 30-31 0.0 0.0 0.0 0.02156954654410896 0.0 32-33 0.0 0.0 0.0 0.04954136132039161 0.0 34-35 0.0 0.0 0.0 0.08913634163722767 0.0 36-37 0.0 0.0 0.0 0.15765585521727699 0.0 38-39 0.0 0.0 0.0 0.26336949852709723 0.0 40-41 0.0 0.0 0.0 0.3907289058599809 0.0 42-43 0.0 0.0 0.0 0.5068081263076061 0.0 44-45 0.0 0.0 0.0 0.6399600620410278 0.0 46-47 0.0 0.0 0.0 0.8072574283460425 0.0 48-49 0.0 0.0 0.0 0.9940664692633963 0.0 50-51 0.0 0.0 0.0 1.2097238259650085 0.0 52-53 0.0 0.0 0.0 1.410252013094163 0.0 54-55 0.0 0.0 0.0 1.6059022815702324 0.0 56-57 0.0 0.0 0.0 1.8413761828000017 0.0 58-59 0.0 0.0 0.0 2.111643363172476 0.0 60-61 0.0 0.0 0.0 2.4172754537798156 0.0 62-63 0.0 0.0 0.0 2.7137614469126206 0.0 64-65 0.0 0.0 0.0 3.0060173699634536 0.0 66-67 0.0 0.0 0.0 3.3314660050989495 0.0 68-69 0.0 0.0 0.0 3.708933069620856 0.0 70-71 0.0 0.0 0.0 4.1308349243732065 0.0 72-73 0.0 0.0 0.0 4.568056492395401 0.0 74-75 0.0 0.0 0.0 4.967016885982462 0.0 76-77 0.0 0.0 0.0 5.39032876409547 0.0 78-79 0.0 0.0 0.0 5.882693678141208 0.0 80-81 0.0 7.621747895444862E-5 0.0 6.441101037700976 0.0 82-83 0.0 7.621747895444862E-5 0.0 7.027899408171276 0.0 84-85 0.0 7.621747895444862E-5 0.0 7.566452114463409 0.0 86-87 0.0 7.621747895444862E-5 0.0 8.103823449831749 0.0 88-89 0.0 7.621747895444862E-5 0.0 8.697824372063245 0.0 90-91 0.0 7.621747895444862E-5 0.0 9.367090054762258 0.0 92-93 0.0 7.621747895444862E-5 0.0 10.07896130819681 0.0 94-95 0.0 7.621747895444862E-5 0.0 10.782105660291075 0.0 96-97 0.0 7.621747895444862E-5 0.0 11.447789121479229 0.0 98-99 0.0 7.621747895444862E-5 0.0 12.143235508199094 0.0 100-101 0.0 7.621747895444862E-5 0.0 12.919777292526495 0.0 102-103 0.0 7.621747895444862E-5 0.0 13.747422896492854 0.0 104-105 0.0 7.621747895444862E-5 0.0 14.5680564923954 0.0 106-107 0.0 7.621747895444862E-5 0.0 15.334690004458722 0.0 108-109 0.0 7.621747895444862E-5 0.0 16.1198062551685 0.0 110-111 0.0 1.1432621843167293E-4 0.0 16.977481545842906 0.0 112-113 0.0 1.5243495790889723E-4 0.0 17.90649639681868 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1760 0.0 67.976654 1 GTATCAA 3425 0.0 54.047577 1 TCAACGC 4195 0.0 43.128895 4 ATCAACG 4240 0.0 43.09226 3 GTACATG 10765 0.0 41.30313 1 CAACGCA 4385 0.0 41.122852 5 TATCAAC 4530 0.0 40.33359 2 AACGCAG 4490 0.0 40.15965 6 TACATGG 11090 0.0 38.63916 2 ACATGGG 11275 0.0 36.632763 3 CATGGGA 9485 0.0 34.636055 4 ATGGGAG 3600 0.0 34.05453 5 ACGCAGA 5560 0.0 32.64142 7 CGCAGAG 5560 0.0 32.637688 8 ATGGGAT 3625 0.0 29.715342 5 GCAGAGT 6240 0.0 28.508928 9 CATGGGG 2205 0.0 27.260859 4 TATAGGG 200 6.102164E-8 26.781769 2 GTATAGA 585 0.0 25.436686 1 TGGTATC 820 0.0 25.402761 2 >>END_MODULE