FastQCFastQC Report
Thu 26 May 2016
SRR1364349_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364349_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312035
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74410.5671342609000523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65170.4967093103461417No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41460.315997667745144No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.10617094818354694No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13500.10289359658850564No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.082.722281
GTATCAA70700.060.8060951
GCGTATC350.00777436151.0366941
ATCAACG89250.047.6269843
TCAACGC90250.047.099264
CAACGCA91550.046.365435
TATCAAC93000.045.8985822
AACGCAG95650.044.359386
GTGGTAT12650.043.774561
TGGTATC12500.043.3405532
GGTGCGT905.150827E-639.6725126
ACGCAGA111050.038.0397767
CGCAGAG112050.037.647198
GTACATG106000.037.635551
TATAACG957.4440577E-637.600252
TACATGG109150.036.1620372
ACATGGG109250.035.4205253
GCAGAGT122400.034.172139
CATGGGA94100.031.8230234
ATGGGAT34550.030.6715015
Produced by FastQC (version 0.11.5)