##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364349_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1312035 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29089010582797 33.0 31.0 34.0 30.0 34.0 2 32.292505916381806 33.0 31.0 34.0 30.0 34.0 3 32.40054571714931 34.0 31.0 34.0 30.0 34.0 4 36.02362284542714 37.0 35.0 37.0 35.0 37.0 5 35.97432080699067 37.0 35.0 37.0 35.0 37.0 6 36.05049865285606 37.0 35.0 37.0 35.0 37.0 7 36.00179797032853 37.0 35.0 37.0 35.0 37.0 8 36.01653080901043 37.0 35.0 37.0 35.0 37.0 9 37.75849882053451 39.0 38.0 39.0 35.0 39.0 10-11 37.720538324053855 39.0 37.5 39.0 35.0 39.0 12-13 37.69045376076096 39.0 37.5 39.0 35.0 39.0 14-15 39.056437518816196 41.0 38.0 41.0 36.0 41.0 16-17 39.073320833666784 41.0 38.0 41.0 36.0 41.0 18-19 39.16231617296795 41.0 39.0 41.0 36.0 41.0 20-21 39.183212719172886 41.0 39.0 41.0 36.0 41.0 22-23 39.131416844825026 41.0 39.0 41.0 35.0 41.0 24-25 39.08737038264985 41.0 39.0 41.0 35.0 41.0 26-27 38.98236327536994 41.0 39.0 41.0 35.0 41.0 28-29 38.88273940862858 41.0 38.0 41.0 35.0 41.0 30-31 38.719253297358684 40.0 38.0 41.0 35.0 41.0 32-33 38.539250858399356 40.0 38.0 41.0 34.0 41.0 34-35 38.4187708407169 40.0 38.0 41.0 34.0 41.0 36-37 38.28247074201526 40.0 38.0 41.0 34.0 41.0 38-39 38.188131414177214 40.0 38.0 41.0 34.0 41.0 40-41 38.06051744046462 40.0 38.0 41.0 33.0 41.0 42-43 37.880783287031214 40.0 37.5 41.0 33.0 41.0 44-45 37.649512779765786 40.0 37.0 41.0 33.0 41.0 46-47 37.303109673141336 40.0 37.0 41.0 31.5 41.0 48-49 37.01311779030285 40.0 37.0 41.0 31.0 41.0 50-51 36.86084631888631 40.0 37.0 41.0 31.0 41.0 52-53 36.731074628344516 40.0 36.0 41.0 31.0 41.0 54-55 36.56820168669281 40.0 36.0 41.0 30.5 41.0 56-57 36.39412744324656 39.5 35.5 41.0 30.0 41.0 58-59 36.19170067871665 39.0 35.0 41.0 30.0 41.0 60-61 35.955480227280525 39.0 35.0 41.0 29.0 41.0 62-63 35.73563853098431 39.0 35.0 41.0 29.0 41.0 64-65 35.46166756222205 38.0 35.0 40.5 29.0 41.0 66-67 35.14237234524994 37.5 35.0 40.0 28.5 41.0 68-69 34.811149854996245 37.0 34.5 40.0 28.0 41.0 70-71 34.54025959673332 36.5 34.0 39.0 28.5 41.0 72-73 34.32982123190311 36.0 34.0 39.0 28.0 41.0 74-75 33.989360039937964 36.0 34.0 39.0 28.0 40.5 76-77 33.64829901641343 35.0 34.0 37.5 28.0 39.5 78-79 33.313847953751235 35.0 34.0 37.0 27.5 39.0 80-81 32.99728437122485 35.0 34.0 36.5 27.0 39.0 82-83 32.705308547409174 35.0 34.0 36.0 26.5 37.5 84-85 32.444945066252046 35.0 34.0 36.0 26.0 37.0 86-87 32.24003589843259 35.0 34.0 35.5 26.0 37.0 88-89 32.06436223119047 35.0 33.0 35.0 26.0 36.0 90-91 31.904431284226412 35.0 33.0 35.0 25.5 36.0 92-93 31.76579092783348 35.0 33.0 35.0 25.0 36.0 94-95 31.669093050109183 35.0 33.0 35.0 25.0 36.0 96-97 31.582290106590143 35.0 33.0 35.0 25.0 35.0 98-99 31.503498382284008 35.0 33.0 35.0 25.0 35.0 100-101 31.44018147381739 35.0 33.0 35.0 25.0 35.0 102-103 30.482605265865622 34.0 31.5 35.0 21.5 35.0 104-105 31.17327319774244 35.0 32.5 35.0 24.0 35.0 106-107 31.442206953320607 35.0 33.0 35.0 25.0 35.0 108-109 31.44518934327209 35.0 33.0 35.0 24.5 35.0 110-111 31.39931556703899 35.0 33.0 35.0 24.0 35.0 112-113 31.3249379780265 35.0 33.0 35.0 24.0 35.0 114-115 31.23420106933123 35.0 33.0 35.0 23.5 35.0 116-117 31.161927082737883 35.0 33.0 35.0 23.0 35.0 118-119 31.048549009744406 35.0 33.0 35.0 20.0 35.0 120-121 30.904068107939196 35.0 33.0 35.0 18.5 35.0 122-123 30.744165742529734 35.0 33.0 35.0 9.5 35.0 124-125 30.06449561177865 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08061137846675592 1101 2 0.1025587856965231 1101 3 0.0809084522476482 1101 4 0.08134713073898325 1101 5 0.16612964773453598 1101 6 0.22834625006078824 1101 7 0.2496458145948779 1101 8 0.22450827990964228 1101 9 0.23316486771138045 1101 10-11 0.2567118487667557 1101 12-13 0.1705990734020375 1101 14-15 0.24598353044529375 1101 16-17 0.28889204589250284 1101 18-19 0.18925499205415264 1101 20-21 0.21991719108454788 1101 22-23 0.2893366481423598 1101 24-25 0.24889764266273318 1101 26-27 0.19280442173715784 1101 28-29 0.1905190716026155 1101 30-31 0.23165647977378967 1101 32-33 0.18799579817255818 1101 34-35 0.11214015862959315 1101 36-37 0.24566843794562487 1101 38-39 0.20288102878210879 1101 40-41 0.28771447262798233 1101 42-43 0.30124380484928537 1101 44-45 0.1951221299903949 1101 46-47 0.2174562451388269 1101 48-49 0.24199252662241122 1101 50-51 0.12202192271985268 1101 52-53 0.10127169941214476 1101 54-55 0.19815477515236068 1101 56-57 0.15046075489591004 1101 58-59 0.05787388446357511 1101 60-61 0.04651929195690485 1101 62-63 0.00711788949892167 1101 64-65 -0.05102323408930687 1101 66-67 -0.120136247559266 1101 68-69 -0.07222755565395289 1101 70-71 -0.2077456114926548 1101 72-73 -0.1022098396853508 1101 74-75 -0.07932678245324354 1101 76-77 -0.11371837417199515 1101 78-79 -0.4942043810394523 1101 80-81 -0.39617925299892676 1101 82-83 -0.33991517446856534 1101 84-85 -0.4019501622730459 1101 86-87 -0.3875010344539227 1101 88-89 -0.44563510542239015 1101 90-91 -0.8129462612773324 1101 92-93 -0.7518565409461502 1101 94-95 -0.4710140891625585 1101 96-97 -0.3315561648032279 1101 98-99 -0.31640791450811534 1101 100-101 -0.4556917179252622 1101 102-103 -0.39631911644035966 1101 104-105 -0.43086152078630846 1101 106-107 -0.36684597366091154 1101 108-109 -0.2474154052964721 1101 110-111 -0.2741089737299234 1101 112-113 -0.33060673182211886 1101 114-115 -0.5252567399751555 1101 116-117 -0.4544223100258513 1101 118-119 -0.3919259594394049 1101 120-121 -0.32837457250280266 1101 122-123 -0.272496441957113 1101 124-125 -0.4637376694854396 1102 1 0.08009574918621354 1102 2 0.0768470244640227 1102 3 0.07251440749445237 1102 4 0.12093489012836756 1102 5 0.08827276871144818 1102 6 0.06790212899160508 1102 7 0.1756005929071378 1102 8 0.19954410971713088 1102 9 0.3289376503126462 1102 10-11 0.291735360604946 1102 12-13 0.30078146904709513 1102 14-15 0.36098485736939523 1102 16-17 0.3660933187112434 1102 18-19 0.36716737621253515 1102 20-21 0.31572811025587555 1102 22-23 0.3559530945167708 1102 24-25 0.3426234770037624 1102 26-27 0.2158160566761964 1102 28-29 0.1975754797319027 1102 30-31 0.3444073205637608 1102 32-33 0.28878803899457495 1102 34-35 0.27085270801788397 1102 36-37 0.3233840545425437 1102 38-39 0.3198028962342434 1102 40-41 0.2555914826180441 1102 42-43 0.3104045803443043 1102 44-45 0.34321214486508467 1102 46-47 0.3057028399144883 1102 48-49 0.27067294130833375 1102 50-51 0.24551142173672247 1102 52-53 0.15554989412118658 1102 54-55 0.1098316215693913 1102 56-57 0.07428888380150767 1102 58-59 0.13747290485363806 1102 60-61 0.13166411236793607 1102 62-63 0.05335069626192279 1102 64-65 0.08374326297674628 1102 66-67 -0.12553135813674743 1102 68-69 -0.0945477331561193 1102 70-71 -0.034271067153255785 1102 72-73 0.07096361143427998 1102 74-75 -0.015122224176131738 1102 76-77 -0.026145668300927127 1102 78-79 0.05026014580855076 1102 80-81 0.012785897603386331 1102 82-83 -0.002630064042485003 1102 84-85 0.07488194283419602 1102 86-87 0.012715986911409516 1102 88-89 -0.06521339208770627 1102 90-91 -0.008664138644409292 1102 92-93 -0.005978789935390694 1102 94-95 -0.03622976646375875 1102 96-97 0.09320980949186719 1102 98-99 0.0727147929769636 1102 100-101 0.09407464916221642 1102 102-103 0.09296807821355202 1102 104-105 -0.017494381597540354 1102 106-107 -0.04753432875208574 1102 108-109 -0.03901961797341258 1102 110-111 0.02075098792782626 1102 112-113 -0.05968139579894327 1102 114-115 -0.1771168476088434 1102 116-117 -0.17521348245256974 1102 118-119 -0.1970907936938744 1102 120-121 -0.15413029791607258 1102 122-123 -0.297492464041472 1102 124-125 -0.364238786872658 1103 1 0.1672679086344857 1103 2 0.16361233835086608 1103 3 0.2185832347676211 1103 4 0.15863710336822123 1103 5 0.04295704555995883 1103 6 0.06387573241174493 1103 7 0.05912538189723904 1103 8 0.22562978259570343 1103 9 0.2749025120511348 1103 10-11 0.33905204918995935 1103 12-13 0.2445346578366312 1103 14-15 0.3129188032435408 1103 16-17 0.32293346469182893 1103 18-19 0.35530961491872404 1103 20-21 0.2410818459777957 1103 22-23 0.4119340816340653 1103 24-25 0.33407944531818856 1103 26-27 0.38671057261793607 1103 28-29 0.39730517483964434 1103 30-31 0.3617796260365935 1103 32-33 0.3891957367325105 1103 34-35 0.29614145840822914 1103 36-37 0.5139250876663795 1103 38-39 0.4883866393453644 1103 40-41 0.3920786845761839 1103 42-43 0.35304378200334696 1103 44-45 0.34160854109307337 1103 46-47 0.31981034720472223 1103 48-49 0.19201856101109627 1103 50-51 0.027212525497738227 1103 52-53 0.17313950460504657 1103 54-55 0.01966103782297779 1103 56-57 0.028115486223235564 1103 58-59 -0.017253348560565485 1103 60-61 0.025569510878661106 1103 62-63 0.2051781750509747 1103 64-65 0.15939825684107234 1103 66-67 0.07039735482380394 1103 68-69 0.1280858551337687 1103 70-71 0.17706788358104575 1103 72-73 0.2720374088207933 1103 74-75 0.30083032773516294 1103 76-77 0.19797730461854712 1103 78-79 0.15270179645796134 1103 80-81 0.19184124069514752 1103 82-83 0.1883934109464107 1103 84-85 0.1164230738208758 1103 86-87 0.1819420237078404 1103 88-89 0.21851795349351022 1103 90-91 0.24530741625334684 1103 92-93 0.1327093640413608 1103 94-95 0.14501319620761066 1103 96-97 0.17354050346043692 1103 98-99 0.19988839287843874 1103 100-101 0.2312046945969719 1103 102-103 0.3298416734967802 1103 104-105 0.3285596562896771 1103 106-107 0.28365830052583973 1103 108-109 0.3242053643233085 1103 110-111 0.2687970296503899 1103 112-113 0.29326386368056134 1103 114-115 0.3813743491328765 1103 116-117 0.3609908243925517 1103 118-119 0.3054027847437517 1103 120-121 0.3348506474170776 1103 122-123 0.33929561120516283 1103 124-125 0.17493301851001775 1104 1 0.1175188506565874 1104 2 0.16835128627163698 1104 3 0.16875077447277675 1104 4 0.119460349261999 1104 5 0.2324456960595782 1104 6 0.24425628446157788 1104 7 0.276079687605467 1104 8 0.25121450351741714 1104 9 0.3207930113115367 1104 10-11 0.2746671568270145 1104 12-13 0.26925609409803286 1104 14-15 0.28706351955127474 1104 16-17 0.34355122423983886 1104 18-19 0.265243452850207 1104 20-21 0.21639249840443853 1104 22-23 0.19894930213282436 1104 24-25 0.28782791297731336 1104 26-27 0.186078948494945 1104 28-29 0.2588235437051907 1104 30-31 0.3211844671028814 1104 32-33 0.26195617248276193 1104 34-35 0.30131805645316945 1104 36-37 0.35955065027862076 1104 38-39 0.29219880375706 1104 40-41 0.14872047756202278 1104 42-43 0.13144114004412444 1104 44-45 0.17940644286041163 1104 46-47 0.13831856530149622 1104 48-49 0.1080277629724975 1104 50-51 0.2190620270063306 1104 52-53 0.09458060051714057 1104 54-55 0.14336999822865693 1104 56-57 0.1484961287433677 1104 58-59 0.18313400572569094 1104 60-61 0.21785425939381753 1104 62-63 0.2529658649820803 1104 64-65 0.0801294435401303 1104 66-67 0.03356335766458329 1104 68-69 -0.03816125547071181 1104 70-71 0.0015280585506332045 1104 72-73 0.14306434861732953 1104 74-75 0.19095738763103043 1104 76-77 0.17036180795916778 1104 78-79 0.15611860561536872 1104 80-81 0.25707612989163664 1104 82-83 0.20218546833172724 1104 84-85 0.15015605974821966 1104 86-87 0.2941396266969818 1104 88-89 0.21626806997403492 1104 90-91 0.14207358329773712 1104 92-93 0.16658370982793258 1104 94-95 0.1268734749918714 1104 96-97 0.06619733939705341 1104 98-99 0.07378719957505453 1104 100-101 0.2177347963308378 1104 102-103 0.19483636402046756 1104 104-105 0.1638358395282431 1104 106-107 0.11633968218841417 1104 108-109 0.1724421262552127 1104 110-111 0.262785277557132 1104 112-113 0.1931181782096374 1104 114-115 0.14908730493360878 1104 116-117 0.13565698894899825 1104 118-119 0.11842468534402428 1104 120-121 -0.016681212833432824 1104 122-123 0.01748819535385948 1104 124-125 -0.11044341714071138 1105 1 0.08423415141694335 1105 2 0.10896261852383304 1105 3 0.06933302794283236 1105 4 0.10272564570120579 1105 5 0.06306009547935787 1105 6 0.06891682018221701 1105 7 0.12613501524165827 1105 8 0.08657369256508929 1105 9 0.11076998213616207 1105 10-11 0.12433273388663224 1105 12-13 0.0944398276035372 1105 14-15 0.14354422435588532 1105 16-17 0.13624077434905502 1105 18-19 0.20925959530234906 1105 20-21 0.08893982344455509 1105 22-23 0.1066716167089865 1105 24-25 0.13059956489276914 1105 26-27 0.1414923298184334 1105 28-29 0.21434919014041043 1105 30-31 0.2347657495856268 1105 32-33 0.13333721128666554 1105 34-35 0.2061502857756281 1105 36-37 0.17202851498771565 1105 38-39 0.16596198113931848 1105 40-41 0.2154127322013295 1105 42-43 0.2648332748774962 1105 44-45 0.24669221984513 1105 46-47 0.3024069471757258 1105 48-49 0.16626327738394053 1105 50-51 0.08926449395802649 1105 52-53 0.13378442481758412 1105 54-55 0.06422351810000038 1105 56-57 0.11256663292810742 1105 58-59 0.06461382088224354 1105 60-61 -0.034265221187283146 1105 62-63 0.006597323615139317 1105 64-65 -0.020532115699417375 1105 66-67 0.04361807525639705 1105 68-69 0.027554080875567877 1105 70-71 0.055086566043158314 1105 72-73 0.12668169150123987 1105 74-75 0.1970866390896262 1105 76-77 0.1563468855957879 1105 78-79 0.2469160861320745 1105 80-81 0.3387089645136925 1105 82-83 0.22308160658133858 1105 84-85 0.18577121325063928 1105 86-87 0.21015388478981123 1105 88-89 0.17334805865432656 1105 90-91 0.20543474143309481 1105 92-93 0.2729216473017715 1105 94-95 0.19990892026496354 1105 96-97 0.17405996418078473 1105 98-99 0.10964721811362566 1105 100-101 0.07547202310297152 1105 102-103 0.2712279006714269 1105 104-105 0.2467785750011373 1105 106-107 0.26795774017990936 1105 108-109 0.21816368166503608 1105 110-111 0.24669662794120484 1105 112-113 0.24618483742020558 1105 114-115 0.133834924489598 1105 116-117 -0.04847855274499224 1105 118-119 -0.12632850933977835 1105 120-121 -0.13829864383487234 1105 122-123 -0.019918539875018126 1105 124-125 -0.17076875719808982 1106 1 0.04535478388045533 1106 2 0.10337112754147881 1106 3 0.07727567868733587 1106 4 0.031525893870650634 1106 5 0.04166162380999339 1106 6 0.05285644866518879 1106 7 0.1447339021648517 1106 8 0.1295840984818426 1106 9 0.17889372286052208 1106 10-11 0.10044028216910306 1106 12-13 0.14242590800061095 1106 14-15 0.23359471823764721 1106 16-17 0.1922700939716364 1106 18-19 0.19023147967013188 1106 20-21 0.1862457414257861 1106 22-23 0.05903414066593626 1106 24-25 0.12536896388042607 1106 26-27 0.1132392522716188 1106 28-29 0.14817425525417605 1106 30-31 0.1375158847274065 1106 32-33 0.24824688129502448 1106 34-35 0.23432811392443398 1106 36-37 0.2771580668084468 1106 38-39 0.12263291705754398 1106 40-41 0.11001289682857873 1106 42-43 0.16004848815578754 1106 44-45 0.17275277564639424 1106 46-47 0.1287827825976109 1106 48-49 0.13048602788765606 1106 50-51 0.06678028097458366 1106 52-53 0.03236282547609193 1106 54-55 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-0.5411719505807326 2216 66-67 -0.6313389250876043 2216 68-69 -0.8353515492127244 2216 70-71 -0.9489726014835043 2216 72-73 -0.9019446128216586 2216 74-75 -0.81776257544999 2216 76-77 -0.8427701881342884 2216 78-79 -0.7949369123772669 2216 80-81 -0.6959487582245245 2216 82-83 -0.7407929490888847 2216 84-85 -0.714750515908861 2216 86-87 -0.7185641521325579 2216 88-89 -0.5929402110395188 2216 90-91 -0.6943645935055542 2216 92-93 -0.5683637557483365 2216 94-95 -0.7013352031153843 2216 96-97 -0.6550192698520121 2216 98-99 -0.6966421982377113 2216 100-101 -0.7864990811281949 2216 102-103 -0.7767081158404423 2216 104-105 -0.8256959118696336 2216 106-107 -0.7863938250075684 2216 108-109 -0.5837799027862864 2216 110-111 -0.4405009411449079 2216 112-113 -0.5145478767180798 2216 114-115 -0.6419534025371192 2216 116-117 -0.6173410685115321 2216 118-119 -0.6361209643334718 2216 120-121 -0.5935446018672081 2216 122-123 -0.6191732125043039 2216 124-125 -0.5178218474400325 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 201.0 3 10.0 4 45.0 5 149.0 6 380.0 7 633.0 8 1146.0 9 1903.0 10 3238.0 11 4328.0 12 5463.0 13 8312.0 14 13444.0 15 11321.0 16 4951.0 17 4165.0 18 4181.0 19 3880.0 20 3673.0 21 3866.0 22 4014.0 23 4241.0 24 5140.0 25 6657.0 26 8584.0 27 11027.0 28 13908.0 29 17725.0 30 22518.0 31 28169.0 32 35703.0 33 46510.0 34 64036.0 35 97561.0 36 186036.0 37 430649.0 38 252770.0 39 1498.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.66890449046307 19.531048089832133 13.413677459096693 22.386369960608103 2 15.544249962843978 21.703994176984608 39.42676834078359 23.324987519387822 3 17.758977466302348 28.07623272245024 30.880273773184403 23.284516038063007 4 11.555941724115591 17.63466675812764 38.614061362692304 32.19533015506446 5 11.557263155647004 40.583880261915624 34.491123818123306 13.367732764314072 6 29.310180544492002 38.780133626554566 18.02449184938999 13.885193979563445 7 26.66262714028208 34.82658618100889 21.93302770124273 16.577758977466303 8 27.06795169336184 34.650904892018886 20.356392931591003 17.924750483028273 9 27.939803435121775 14.602887880277585 20.11104886683663 37.34625981776401 10-11 24.06681224205147 27.701852465825986 27.978674349388545 20.252660942733996 12-13 25.438536319534162 25.594896477609208 28.673129908882007 20.293437293974627 14-15 22.349518114989312 25.867259638652936 27.388446192365297 24.394776053992462 16-17 20.690111163193055 29.811857153200943 27.90051332472076 21.597518358885242 18-19 20.921354994340852 26.932627559478217 31.379726912772902 20.76629053340803 20-21 22.0395920221458 26.280951988975882 31.359029352942613 20.32042663593571 22-23 21.534219742613573 26.64955584264139 31.22454812562165 20.591676289123384 24-25 20.9970770596821 26.832020487258347 31.486240839611746 20.684661613447812 26-27 20.94865245026884 27.094055430918285 31.158217098014944 20.799075020797932 28-29 20.83164860626547 26.765762281041056 31.57143592098519 20.831153191708285 30-31 20.79557389518869 26.541499686745922 31.71106846316483 20.95185795490056 32-33 20.7520457731866 26.791711650875033 31.25950575232587 21.19673682361249 34-35 21.19634993797791 26.68310426761532 31.164624351912014 20.955921442494756 36-37 21.518252180772617 26.254253888044143 30.840145270514885 21.38734866066835 38-39 21.177636267325187 26.400400903939303 30.98674959128377 21.435213237451745 40-41 21.133125259615788 26.417930924098826 31.211514936720437 21.237428879564952 42-43 21.38113693613356 26.302918748356564 30.840030944296455 21.47591337121342 44-45 21.091777277283 26.60454942131879 30.745864249048232 21.557809052349974 46-47 21.769922296280207 26.894061515127266 29.755837306169425 21.580178882423105 48-49 21.645743570825907 26.967013636152302 29.420202480589197 21.967040312432594 50-51 21.740807219319606 26.9661251414787 29.332944624190667 21.960123015011032 52-53 21.89148917521255 27.079231880247097 29.228145590628294 21.801133353912054 54-55 21.250690720903027 27.23376281882724 29.424291272717575 22.09125518755216 56-57 21.50861066968488 27.230409249753247 29.358858567035178 21.902121513526698 58-59 21.489199780340975 26.607361778491175 29.7123129859108 22.191125455257048 60-61 21.2234210393938 26.932419434252463 29.870414943515183 21.973744582838556 62-63 21.237505097043904 27.04085637959353 29.830759087981644 21.890879435380917 64-65 20.90862667535546 26.799094536350022 29.99272123075985 22.29955755753467 66-67 21.03945397798077 27.13189815820462 29.536978815351723 22.291669048462882 68-69 21.119977744496147 26.796274489628708 29.657897845712956 22.425849920162193 70-71 21.02947750230653 26.914764817541005 29.50273030168033 22.553027378472137 72-73 21.113553459742658 26.944881001559413 29.223175618924508 22.718389919773422 74-75 20.901386014854786 27.213870056820134 29.03828785055277 22.846456077772316 76-77 21.06693840748852 26.97974024311098 29.244734037100393 22.708587312300097 78-79 21.248602837806477 26.988962561578983 28.96059516727647 22.801839433338074 80-81 21.15924001853613 27.49094534279652 28.689772810419257 22.660041828248094 82-83 21.137965069529397 27.123742887956492 28.80734888932079 22.930943153193322 84-85 21.02790702991917 26.85416166489461 28.942368153288594 23.175563151897624 86-87 21.11288951895338 26.671887563975044 28.887301024744005 23.32792189232757 88-89 21.04932414150537 26.822417084910082 28.829375740738623 23.29888303284592 90-91 20.92371773618844 27.060672924121686 28.76706795169336 23.24854138799651 92-93 21.034194971932912 26.532028490093634 28.796564116048735 23.63721242192472 94-95 20.677306626728708 26.32349746767426 29.027388751062283 23.971807154534748 96-97 20.98812150590495 26.28817066617887 28.923656762205276 23.8000510657109 98-99 20.823593344974316 26.43790428732248 28.658469197755238 24.080033169947967 100-101 20.439584309869783 26.36964715118118 28.97636114890228 24.21440739004676 102-103 20.59887884088458 25.88951514250763 29.00684814048406 24.50475787612373 104-105 20.320407067430832 26.060760576165652 28.904737242653212 24.7140951137503 106-107 20.4083203668199 26.054936859462813 28.9304731929482 24.606269580769087 108-109 20.6090026478376 26.027923482523814 28.52846844622085 24.834605423417738 110-111 20.239003983653795 26.389448367037318 28.696751071752647 24.67479657755625 112-113 20.06657596786671 26.271326603329943 28.78471229807132 24.87738513073203 114-115 20.094585891382472 26.126322849619104 28.628047270080447 25.151043988917976 116-117 19.76986885634486 26.300108724275162 28.752978675484524 25.177043743895457 118-119 19.61712149447233 26.511830858170704 28.38556136078687 25.485486286570097 120-121 19.60200574834855 26.63809260313606 28.53050504219038 25.22939660632501 122-123 19.530779294473092 26.613982263315567 28.504378531988493 25.35085991022285 124-125 19.416555198603696 26.625509228031262 28.428852888832996 25.529082684532046 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 534.0 1 393.5 2 497.0 3 733.5 4 479.0 5 349.5 6 791.5 7 1954.0 8 3468.0 9 3996.5 10 2869.0 11 1678.5 12 1475.0 13 1560.5 14 1773.5 15 1935.0 16 2009.5 17 2151.0 18 2325.5 19 2389.0 20 2542.0 21 2918.5 22 3417.5 23 4266.5 24 5331.5 25 6419.5 26 8123.0 27 10241.0 28 11994.0 29 14956.0 30 18839.5 31 21033.0 32 23903.0 33 26810.5 34 29530.0 35 31895.5 36 35383.5 37 40213.5 38 41769.0 39 42933.0 40 44281.5 41 45762.0 42 46369.0 43 46433.0 44 47793.5 45 49764.0 46 48482.5 47 45905.5 48 46001.0 49 45124.0 50 43031.0 51 40379.0 52 39952.0 53 40461.0 54 38687.0 55 35883.0 56 31665.0 57 27617.0 58 26107.5 59 23961.0 60 20010.5 61 16157.0 62 12906.5 63 9807.5 64 7002.5 65 5119.0 66 3656.0 67 2955.0 68 1998.0 69 900.5 70 577.5 71 453.0 72 356.5 73 263.0 74 202.5 75 186.5 76 121.0 77 48.0 78 23.5 79 7.0 80 2.0 81 1.0 82 1.0 83 0.5 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026218812760330324 2 0.0 3 0.0 4 0.0 5 0.05891611123178879 6 0.012956971422256266 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.2865243686334585E-4 22-23 0.0 24-25 0.0 26-27 4.1919613424946743E-4 28-29 1.9054369738612156E-4 30-31 7.621747895444862E-5 32-33 3.810873947722431E-5 34-35 1.9054369738612156E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 6.097398316355889E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.1432621843167293E-4 60-61 7.621747895444862E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 3.810873947722431E-5 72-73 7.621747895444862E-5 74-75 0.0 76-77 3.810873947722431E-5 78-79 3.810873947722431E-5 80-81 2.2865243686334585E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 3.0486991581779447E-4 100-101 0.0 102-103 0.0 104-105 0.0012575884027484024 106-107 1.1432621843167293E-4 108-109 0.0016005670580434212 110-111 0.0036965477292907585 112-113 0.0 114-115 0.0 116-117 3.810873947722431E-5 118-119 0.0 120-121 4.573048737266917E-4 122-123 6.478485711128133E-4 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1312035.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.47057407262163 #Duplication Level Percentage of deduplicated Percentage of total 1 76.59551092599412 38.658194078206854 2 12.048055731394093 12.161445784447983 3 4.385922117114998 6.6408002126580605 4 2.1264641527356063 4.2929546613366805 5 1.1929531756650282 3.010451580878551 6 0.7685612680712001 2.327383704572131 7 0.5130486873162156 1.8125703243238045 8 0.3731974128730946 1.5068390136097822 9 0.25666142491677546 1.165846451206206 >10 1.5416513776445524 14.380960807554896 >50 0.1307604364754597 4.500957438851713 >100 0.0638526253089115 5.7337466890178455 >500 0.0018330897217865713 0.593342940953022 >1k 9.165448608932856E-4 1.0622503950689623 >5k 6.110299072621904E-4 2.1522559173134823 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7441 0.5671342609000523 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6517 0.4967093103461417 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4146 0.315997667745144 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1393 0.10617094818354694 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.10289359658850564 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.810873947722431E-5 0.0 20-21 0.0 0.0 0.0 1.1432621843167293E-4 0.0 22-23 0.0 0.0 0.0 5.335223526811404E-4 0.0 24-25 0.0 0.0 0.0 0.0016386757975206455 0.0 26-27 0.0 0.0 0.0 0.003201134116086842 0.0 28-29 0.0 0.0 0.0 0.008117161508648777 0.0 30-31 0.0 0.0 0.0 0.02172198150201786 0.0 32-33 0.0 0.0 0.0 0.049770013757254955 0.0 34-35 0.0 0.0 0.0 0.08978419020834048 0.0 36-37 0.0 0.0 0.0 0.15807505135152644 0.0 38-39 0.0 0.0 0.0 0.2636362597034378 0.0 40-41 0.0 0.0 0.0 0.3914148631705709 0.0 42-43 0.0 0.0 0.0 0.5077608447945368 0.0 44-45 0.0 0.0 0.0 0.6411033242253446 0.0 46-47 0.0 0.0 0.0 0.8074098633039515 0.0 48-49 0.0 0.0 0.0 0.9943332304397368 0.0 50-51 0.0 0.0 0.0 1.210333565796644 0.0 52-53 0.0 0.0 0.0 1.4116620364548202 0.0 54-55 0.0 0.0 0.0 1.608036370980957 0.0 56-57 0.0 0.0 0.0 1.8441962295213161 0.0 58-59 0.0 0.0 0.0 2.1152636934228126 0.0 60-61 0.0 0.0 0.0 2.4208576752906743 0.0 62-63 0.0 0.0 0.0 2.71829638691041 0.0 64-65 0.0 0.0 0.0 3.012152877019287 0.0 66-67 0.0 0.0 0.0 3.3380969257679864 0.0 68-69 0.0 0.0 0.0 3.717698079700618 0.0 70-71 0.0 0.0 0.0 4.141581588905784 0.0 72-73 0.0 0.0 0.0 4.580098854070204 0.0 74-75 0.0 0.0 0.0 4.980088183623151 0.0 76-77 0.0 0.0 0.0 5.404619541399429 0.0 78-79 0.0 0.0 0.0 5.899995045863868 0.0 80-81 0.0 0.0 0.0 6.461603539539722 0.0 82-83 0.0 0.0 0.0 7.051526826647155 0.0 84-85 0.0 0.0 0.0 7.594423929239692 0.0 86-87 0.0 0.0 0.0 8.13610155216896 0.0 88-89 0.0 0.0 0.0 8.735056610532492 0.0 90-91 0.0 0.0 0.0 9.40512257676053 0.0 92-93 0.0 0.0 0.0 10.118327636076781 0.0 94-95 0.0 0.0 0.0 10.824215817413407 0.0 96-97 0.0 0.0 0.0 11.492566890364968 0.0 98-99 0.0 0.0 0.0 12.190909541285102 0.0 100-101 0.0 0.0 0.0 12.970804894686498 0.0 102-103 0.0 0.0 0.0 13.800660805542535 0.0 104-105 0.0 0.0 0.0 14.622399554889924 0.0 106-107 0.0 0.0 0.0 15.391395808800832 0.0 108-109 0.0 0.0 0.0 16.181046999508396 0.0 110-111 0.0 0.0 0.0 17.041923424298894 0.0 112-113 0.0 0.0 0.0 17.97394886569337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3455 0.0 82.72228 1 GTATCAA 7070 0.0 60.806095 1 GCGTATC 35 0.007774361 51.036694 1 ATCAACG 8925 0.0 47.626984 3 TCAACGC 9025 0.0 47.09926 4 CAACGCA 9155 0.0 46.36543 5 TATCAAC 9300 0.0 45.898582 2 AACGCAG 9565 0.0 44.35938 6 GTGGTAT 1265 0.0 43.77456 1 TGGTATC 1250 0.0 43.340553 2 GGTGCGT 90 5.150827E-6 39.672512 6 ACGCAGA 11105 0.0 38.039776 7 CGCAGAG 11205 0.0 37.64719 8 GTACATG 10600 0.0 37.63555 1 TATAACG 95 7.4440577E-6 37.60025 2 TACATGG 10915 0.0 36.162037 2 ACATGGG 10925 0.0 35.420525 3 GCAGAGT 12240 0.0 34.17213 9 CATGGGA 9410 0.0 31.823023 4 ATGGGAT 3455 0.0 30.671501 5 >>END_MODULE