FastQCFastQC Report
Thu 26 May 2016
SRR1364348_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364348_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191700
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17550.046.475721
GTATCAA25550.045.2057341
ATCAACG27100.041.0710683
CAACGCA28800.038.440075
AACGCAG29500.037.927136
TCAACGC29550.037.464434
GTACATG71350.036.3810461
TATCAAC31950.035.395372
TACATGG72600.034.843042
ACATGGG73400.033.3280143
ACGCAGA37600.029.5902757
CGCAGAG38450.028.9348138
CATGGGG51750.028.8686894
TATTCCG2853.6379788E-1227.149525
TAAGGTG10200.026.2588715
ATTCCGT3051.0913936E-1125.366336
GGACCGA5350.023.3603696
TCCGTTG3103.3833203E-1023.0300568
CAATACA15050.022.1470174
GAGTACA55450.022.0107561
Produced by FastQC (version 0.11.5)