FastQCFastQC Report
Thu 26 May 2016
SRR1364348_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364348_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191700
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.10836337089930191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.10074371492448783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.063.7329751
GTATCAA43950.051.0878871
ATCAACG55100.040.2001463
CAACGCA57000.038.860145
TATCAAC57400.038.6930732
TCAACGC58150.038.091634
AACGCAG59250.037.666156
GTACATG73350.032.6408461
TACATGG73200.032.0495152
ACATGGG74400.030.7322673
CGCAGAG72400.030.652768
ACGCAGA72850.030.5450887
TATTCCG2502.1827873E-1128.581015
CATGGGG52500.026.3126754
GCAGAGT83500.026.2929449
CAGAGTA81850.023.18842710-11
ATTCCGT3103.3651304E-1023.0371046
GAGTACT49500.023.01775212-13
AGAGTAC80850.022.996910-11
GTGGTAT18500.022.5352171
Produced by FastQC (version 0.11.5)